107 results about "Amylase inhibitors" patented technology

The invention relates to compositions comprising at least two inhibitors selected from the group consisting of an alpha amylase inhibitor, an alpha glucosidase inhibitor, and a sodium dependent glucose transporter inhibitor. The invention also provides methods of using the compositions for controlling glucose uptake.

The invention discloses a method for industrially extracting an alpha-amylase inhibitor in white kidney beans, belonging to the field of preparation of plant effective components. The method comprises the following steps: firstly adding water into soaked and peeled white kidney beans, grinding into slurry, sieving to remove residues, performing standing, centrifuging, enzyme deactivation, centrifuging and ethanol precipitation on an obtained filtrate to obtain protein, dissolving the obtained protein by using a phosphate buffer solution, performing moderate enzyme hydrolysis by using alkaline protease to change isoelectric points of impurity protein, then performing acid precipitation, collecting supernatant liquid, and performing ethanol precipitation and freeze drying to obtain an alpha-amylase inhibitor product. The prepared alpha-amylase inhibitor disclosed by the invention is high in purity and strong in activity; and moreover, the alpha-amylase inhibitor needs simple equipment, is low in cost, is pollution-free, has relatively good economic and environmental benefits, and can be applied to industrial large-scale production.

A white kidney bean extract extracted by microwave is disclosed, wherein the bioactivity of an alpha-amylase inhibitor contained in the extract is equal to or higher than 4100 U / mg, and the HPLC (high-performance liquid chromatography) content is equal to or higher than 20% w / w. Preparation method for the extract is characterized by comprising the following steps of: crushing white kidney beans and passing through a 60-mesh sieve; preparing a mixture which is uniformly mixed by the crushed and sieved white kidney bean powder and purified water in a weight ratio of 1:5, extracting for 1-10 minutes at a temperature below 40 DEG C in microwave extraction equipment, then filtering to obtain a filtrate, and removing the filter residue; heating the filtrate to 70 DEG C in a water bath with stirring, keeping for 20 minutes, and collecting an effluent through a silica gel column; and drying the effluent to prepare the white kidney bean extract. The extract can be used for preparing an appetite inhibitor. The preparation method disclosed by the invention shortens extraction time and effectively increases extraction rate. The purified water is used as an extraction solvent, as well as is safe and environment-friendly.

The present invention provides a plant-derived carbohydrase inhibitor, wherein the inhibitor is effective for preventing or alleviating diabetes, or preventing obesity, and foods, drinks, and medicines containing the same. The present invention is accomplished by use of an α-amylase inhibitor or an α-glucosidase inhibitor as a carbohydrase inhibitor that is extracted from astringent skins of a chestnut using ethanol or aqueous ethanol solution. The present invention can also be accomplished by adding the carbohydrase inhibitor to a food or medical composition as an active ingredient for delaying saccharide digestion or absorption, suppressing a rise in postprandial blood glucose levels or blood insulin levels, or preventing obesity.

The invention discloses an industrial preparation method of a safe and efficient white kidney bean alpha-amylase inhibitor and belongs to the field of preparation of active plant ingredients. The industrial preparation method of the safe and efficient white kidney bean alpha-amylase inhibitor comprises the following steps: firstly grinding white kidney beans into powder, extracting by alkaline water, then adjusting supernatant liquor prepared by centrifuging till the supernatant liquor is acidic, carrying out enzyme deactivation, alkali treatment and ultra-filtration to obtain a retention liquid, and drying the retention liquid to obtain the white kidney bean alpha-amylase inhibitor product free of phytolectins and trypsin inhibitors. The alpha-amylase inhibitor product prepared by the method is free of activity of phytolectins and trypsin inhibitors; the alpha-amylase inhibitor product is high in activity, so that the white kidney bean alpha-amylase inhibitor product is safe and efficient; meanwhile, the industrial preparation method of the safe and efficient white kidney bean alpha-amylase inhibitor is simple in process and low in cost, is applicable to industrial large-scale production, and has high economic and social benefits.

The invention discloses a method for industrially preparing a white kidney bean Alpha-amylase inhibitor, and belongs to the field of the plant active ingredient preparation. The method comprises the following steps: firstly peeling white kidney beans by a dry method, dry-smashing, sieving and preparing bean flour, and extracting the bean flour by using water, collecting slurry after centrifuging,after performing adequate enzymolysis on the slurry by using Beta-amylase, to obtain enzymatic hydrolysate, filtering the enzymatic hydrolysate by using a ceramic film, performing ultrafiltration on obtained filtrate by using 80-150 kDa of an ultrafiltration membrane, performing the ultrafiltration on penetrated liquid by using 15-30 kDa of the ultrafiltration membrane, spray-drying trapped fluid,to obtain the white kidney bean Alpha-amylase inhibitor. The prepared white kidney bean Alpha-amylase inhibitor product is high in Alpha-amylase inhibitor activity, no starch, and no phytolectin andtrypsin inhibitor activity, and has the reliable physiological efficacy and safety. In addition, the preparation technology is green and environmental, capable of adapting the requirements of the modern industrial production, and has the remarkable economic benefit and the social benefit.

The invention discloses a method for extracting an alpha-amylase inhibitor from white kidney beans by use of a microwave-compound enzyme coupling method. According to the method, the white kidney beans are used as raw materials; the alpha-amylase inhibitor in the white kidney beans can be dissolved and separated out from the white kidney beans in an acceleration manner through microwave-compound enzyme coupling treatment after the white kidney beans are smashed; meanwhile, impurities such as fat, proteins and saccharides can be removed from the white kidney beans by only dozens of minutes through enzyme hydrolysis by one step under microwave-compound enzyme coupling catalytic hydrolysis, so that the purity of the alpha-amylase inhibitor in a coarse extract is much higher than that of the alpha-amylase inhibitor extracted by the common coarse extraction method; a pure high-activity alpha-amylase inhibitor can be obtained through ultrafiltration and ion column chromatographic refining. According to the method disclosed by the invention, the extraction condition is mild; water is used as a solvent, so that the pure nature of the product can be guaranteed; furthermore, due to the microwave-compound enzyme coupling method, the time for extracting the pure product is greatly shortened, and the inhibition activity of the product is over 90 percent; basic raw materials can be supplied to promotion of the development of a sugar reduction and fat reduction market in China.

The invention belongs to the field of food and discloses a dietary supplement solid beverage suitable for diabetic population and a preparation method of the dietary supplement solid beverage. The solid beverage is prepared from, by weight, 35%-60% of water-soluble dietary fibers, 15%-40% of white kidney bean extract, 5%-10% of inulin, 3%-8% of hawthorn powder and 3%-8% of apple powder. The white kidney bean extract comprises an effective component alpha- amylase inhibitor which is capable of effectively inhibiting activity of amylopsin and saliva in oral cavity and gastrointestinal tract and hindering hydrolysis and digestion of saccharides in food to reduce blood glucose. The water-soluble dietary fibers are capable of improving an intestinal microenvironment to benefit growth of intestinal beneficial bacteria and slowing digestion of food in gastrointestinal tract to alleviate sharp rise of postprandial blood sugar. Due to addition of the apple powder and the hawthorn powder, taste improvement is realized. The dietary supplement solid beverage is provided for diabetic and potential diabetic population to drink in daily life.

The present invention provides a plant-derived carbohydrase inhibitor, wherein the inhibitor is effective for preventing or alleviating diabetes, or preventing obesity, and foods, drinks, and medicines containing the same. The present invention is accomplished by use of an alpha-amylase inhibitor or an alpha-glucosidase inhibitor as a carbohydrase inhibitor that is extracted from astringent skins of a chestnut using ethanol or aqueous ethanol solution. The present invention can also be accomplished by adding the carbohydrase inhibitor to a food or medical composition as an active ingredient for delaying saccharide digestion or absorption, suppressing a rise in postprandial blood glucose levels or blood insulin levels, or preventing obesity.

The invention relates to an extraction process for amylase inhibitor. A kidney bean as a raw material bean is pulverized to be ultrathin, the powdered kidney bean is subjected to primary extraction in de-ionized water, then the primary extract is separated, filtered and dried, and then the primary extract is extracted in a supercritical carbon dioxide device. By adopting the process to extract amylase inhibiting protein, the yield of a finished product can achieve 14 to 19 percent, the cost is lower, the process period is shorter, and the product is safe and environment-friendly.

Extract obtainable by extraction from Phaseolus sp. with mixtures of ethanol and water, characterised by an α-amylase inhibitor content in between 1,000 and 1,600 USP / mg (HPLC titre between 6 and 14% w / w) and a phytohaemagglutinin content in between 8,000 and 30,000 HAU / g, and a process for its preparation.

This invention relates to the combination of α-amylase inhibitors prepared from Phaseolus vulgaris with α-glucosidase inhibitors obtained from Salacia oblonga and other species. The α-amylase inhibitor is accompanied by a quantity of lectins that reduces the amount of glucose originating from the starches present in the diet, and considerably reduces the appetite after repeated administration. The combination with α-glucosidase inhibitors, such as extracts of Salacia or the thiosugars present in it, further reduce the blood glucose, acting synergically, and consequently reduce the synthesis of fats from carbohydrates.

The invention discloses a weight management combination for a lunch and a dinner respectively. The weight management combination is characterized by including a lunch fat-reducing composition and a dinner substitute composition, the lunch fat-reducing composition includes an amylase inhibitor, a lipase inhibitor and a fat burning promoter, the amylase inhibitor includes a white kidney bean extract, the lipase inhibitor includes EGCG, and a lipid absorption conversion inhibitor includes chitosan oligosaccharide; and the dinner substitute composition is prepared from the following components inparts by weight: 25-40 parts of casein, 12-35 parts of a whey protein, 10-15 parts of medium chain triglyceride powder, 20-25 parts of a dietary fiber, 2-5 parts of skim milk powder, 2-5 parts of complex gum and 1-3 parts of decavitamin and mineral substances. According to the weight management combination, the lunch and the dinner are organically combined, collocation is reasonable, pursuit of food can be realized, the purpose of weight loss can also be achieved on the basis of satisfying nutrition demands, and the fast diet weight management combination is provided for a great deal of lose weight people.

The present invention provides a phaseolus vulgaris alpha-amylase inhibitor extraction separation method, and relates to the field of food science biological active component extraction and separation, wherein the obtaining rate can be increased, and the yield can be improved. The method comprises: crushing phaseolus vulgaris to obtain phaseolus vulgaris crude powder, carrying out stirring extraction on the phaseolus vulgaris crude powder 3 times with water at a room temperature, filtering to obtain a filtrate, carrying out pressure reducing concentration on the filtrate to obtain a concentrated solution, carrying out boiling sterilization on the concentrated solution, adding a glucono delta-lactone coagulant, precipitating, filtering to obtain a precipitate, and sequentially carrying out decolorizing, dewatering, filtration draining, vacuum drying, and crushing so as to obtain the phaseolus vulgaris alpha-amylase inhibitor product.

The invention discloses a method for preparing an alpha-amylase inhibitor by an enzymatic method. In the method, white kidney beans are used as raw materials and are subjected to soaking, grinding and centrifugating; supernatant fluid is processed by steps by complex enzyme and is subjected to ion-exchange chromatography and hyperfiltration to prepare the alpha-amylase inhibitor. In the method, the white kidney beans are used as raw materials and the alpha-amylase inhibitor is prepared by the enzymatic method, therefore, the alpha-amylase inhibitor contained in the white kidney beans is effectively released to conduce to extraction and purification, thus reducing extraction cost. The method has mild reaction conditions, high purity of obtained products, good effect on inhibiting the amylase and inhibition activity as high as 70-95%, and can be widely applied in such fields as medicines, health-care food and the like.

The invention discloses an alpha-amylase inhibitor producing strain, i.e. Actinoplanes sp.ZJB-08196 preserved in the China Center for Type Culture Collection located in Wuhan university, Wuhan Province, China on Feb. 16th, 2009 with the preservation number of CCTCC No. M209022. The alpha-amylase inhibitor is acarbose. The invention also discloses a screening method of the alpha-amylase inhibitor producing strain. The screening method provided by the invention is simple and effective, can screen a great amount of samples in a short time, has high sensitivity, reduces the long processes for processing and analyzing samples through HPLC (High-Performance Liquid Chromatography), and the like and improves detection efficiency.

The invention features the treatment of gastrointestinal disorders associated with an innate immune response tiggered by alpha amylase inhibitor CM3, alpha amylase inhibitor 0.19 (0.19), CM1, CM2, CMa, CMd, CM16, CMb, CMX1 / CMX3, CMX2, and / or alpha amylase inhibitor 0.53 (0.53). To this end, the invention features pharmaceutical compositions including neutralizing antibodies to CM3, 0.19, CM1, CM2, CMa, CMd, CM16, CMb, CMX1 / CMX3, CMX2, and / or 0.53, food products containing reduced levels of CM3, 0.19, CM1, CM2, CMa, CMd, CM16, CMb, CMX1 / CMX3, CMX2, and / or 0.53 protein, the use of oral TLR4 inhibitors to block the effect of said alpha-amylase inhibitors, assays for identifying CM3, 0.19, CM1, CM2, CMa, CMd, CM16, CMb, CMX1 / CMX3, CMX2, and / or 0.53 content in food products, and assays for diagnosing subjects with a disorder related to CM3, 0.19, CM1, CM2, CMa, CMd, CM16, CMb, CMX1 / CMX3, CMX2, and / or 0.53 triggered innate immune responses.

The invention discloses a method for extracting an alpha-amylase inhibitor from hulless oats. The method comprises the following steps of: decladding and pulverizing a hulless oat seed; degreasing with acetone; and then carrying out ammonium sulfate precipitation, ion-exchange chromatography and gel chromatography to obtain an electrophoretic pure alpha-amylase inhibitor, wherein the inhibiting specific activity is 26.43U / mg. A material for extracting the alpha-amylase inhibitor is derived from the hulless oat, a purifying method is simple and convenient, a product has high purity and high inhibiting activity, and the extracted alpha-amylase inhibitor can be used for preparing a medicament for reducing weight and treating diabetes mellitus.

The invention discloses a double-water phase extracting system and a method of separating and purifying alpha-amylase inhibitor by it. And its phase forming reagents includes polyglycol and fructose-1, 6-diphosphoric metal salt that have stabilizing and protecting effect on the bioactivity of biochemical product. It applies the double-water phase system to the process of separate-extracting alpha-amylase inhibitor, uses the advantages of the double-water phase system: fast to separate phase, simple and convenient to operate and easy to amplify.

The invention discloses protein microcapsules for targeted inhibition of small intestinal amylase and a preparation method, and belongs to the field of nutrition and health. The microcapsules are prepared from protein alpha-amylase inhibitor extracts in cereals or legume crops as a core material, and microencapsulation of the core material is achieved by combination with a specific water-in-oil emulsion-electroporation technology. Microcapsule powder products which have high embedding efficiency, uniform morphology and small particle size, and can release in the small intestine in a targetedmanner are prepared. The cereal extracts prepared by the method have good slow-release effect, and have obvious inhibitory effect on small intestinal alpha-amylase, thereby having the functions of lowering blood sugar and losing weight. The products can be applied to the fields of common food, health food, formula food for special medical use and the like, and provides a new solution for food of diabetic and obese people.

The present invention relates to a dietary supplement and methods of use thereof. More particularly, the invention relates to a composition including a kiwifruit extract in combination with an amylase inhibitor and / or a bifunctional protease-amylase inhibitor, particularly, but not exclusively, for managing gut health or for the treatment or prevention of digestive dysfunction, gastrointestinal disorders or symptoms thereof.

Extracts obtainable by extraction from Phaseolus sp. with aqueous solutions, characterised by an α-amylase inhibitor content with an activity equal to or greater than 1,800 USP / mg (HPLC titre equal to or greater than 15% w / w) and a phytohaemagglutinin content of between 1,500 and 6,000 HAU / g, and a process for its preparation.

The invention discloses a method of extracting Alpha amylase inhibitor from kidney beans. The method is as follows: the crushed kidney beans are first dispersed in a buffer solution with a definite pH value, and then shifted into an ultrasonic device for ultrasonic extraction, and then conducted enzyme elimination, centrifugalization, dialysis and ethanol sediment, dried and finally the Alpha amylase inhibitor is obtained. The Alpha amylase inhibitor extracted from kidney beans in the invention is a white solid powder with a protein content of 80 to 95 percent and an inhibitory activity of 5,000 to 50,000 U / g. Compared with the prior method of lixiviation and extraction, the method of extracting Alpha amylase inhibitor from kidney beans has the characteristics of high speed, high efficiency, energy conservation and small pollution. The one-time extracted Alpha amylase inhibitor has high content and strong activity, thereby avoiding the fact that circulative extractions consume a large quantity of water and energy. The method of extracting Alpha amylase inhibitor from kidney beans is a novel green environment-friendly technology with a wide range of application prospects.

The present invention provides a purified amylase inhibitor obtained by a superior process that includes extracting ground beans with supercritical carbon dioxide. The invention also provides a method for inducing weight loss in a mammal in need thereof comprising administering to the mammal, an effective amount of an amylase inhibitor obtained by the superior process. A method for improving post-prandial glucose tolerance in a diabetic mammal comprising administering to the mammal, an effective amount of an amylase inhibitor obtained by the superior process, is also provided.

The invention provides a dietary substance supplementing composition, chewable tablets and a preparation method and application of the chewable tablets, and belongs to the technical field of dietary substance supplements. The dietary substance supplementing composition is prepared from, by mass, 20-40 parts of a white kidney bean extract, 15-35 parts of sorbitol, 10-25 parts of resistant dextrin and 1-3 parts of L-arabinose; the white kidney bean extract comprises an alpha-amylase inhibitor, and the mass percentage of the alpha-amylase inhibitor is greater than or equal to 55%. The dietary substance supplementing composition has the functions of reducing the postprandial blood glucose and managing the weight.

This invention relates to the combination of α-amylase inhibitors prepared from Phaseolus vulgaris with α-glucosidase inhibitors obtained from Salacia oblonga and other species. The α-amylase inhibitor is accompanied by a quantity of lectins that reduces the amount of glucose originating from the starches present in the diet, and considerably reduces the appetite after repeated administration. The combination with α-glucosidase inhibitors, such as extracts of Salacia or the thiosugars present in it, further reduce the blood glucose, acting synergically, and consequently reduce the synthesis of fats from carbohydrates.

The invention relates to a special medical food applicable to diabetics and a production technique thereof. The special medical food applicable to diabetics comprises the following components in parts by weight: 10-20 parts of dietary fiber block, 7-15 parts of algae, 5-15 parts of shredded orange peel, 10-20 parts of peanut kernel, 10-20 parts of walnut kernel, 5-10 parts of white kidney bean extract, 4-12 parts of tuckahoe, 4-10 parts of Lycium barbarum, 4-10 parts of semen dolichoris album, 6-12 parts of red bean, 20-40 parts of grain, 10-20 parts of pumpkin, 3-10 parts of chitin, 2-7 parts of concentrated whey protein, 4-12 parts of Polygonatum sibiricum, 4-12 parts of radix polygonati officinalis, 1-3 parts of Spirulina, 1-3 parts of cereus, 10-15 parts of balsam pear, 0.1-0.3 part of tea polyphenol, 0.05-0.1 part of zinc gluconate, 0.5-2 parts of milk calcium, 0.5-1 part of magnesium carbonate and 0.5-2 parts of sodium erythorbate. The special medical food applicable to diabetics has abundant nutrition, and is especially suitable for diabetics; and when the food is eaten before meals, the added alpha-amylase inhibitor can prevent calorie intake of most starch without influencing the absorption of other nutrient substances, thereby reducing the maximal fat source of the human body.

The invention provides a high-efficiency weight loss composition and its application in weight loss. In order to achieve the above object, the high-efficiency weight loss composition of the present invention comprises the following components: 0.1-5 parts of traditional Chinese medicine powder, 0.1-1 part of DHA, 0.1-5 parts of turmeric powder, 0.1-1 part of resveratrol, L-carnitine 0.1 to 3 parts, 0.1 to 3 parts of Baiyun bean extract, 0.1 to 3 parts of coconut oil, 0.1 to 1 part of α-lipoic acid, 0.1 to 1 part of vitamin D, 1 to 10 parts of dried meat floss, 0.1 to 1 part of aspartame 0.1-3 parts of stevia, 0.1-10 parts of amylase inhibitor, 1-10 parts of food thickener, 1-10 parts of dehydrated vegetables, 1-10 parts of nut pieces, 1-10 parts of whey protein powder , Konjac powder 1 to 10 parts. The present invention directly consumes human body fat through the natural ketone metabolism of the human body, does not require a lot of exercise and diet, is suitable for use by social groups, and can effectively alleviate the symptoms of obesity-related diseases such as hyperlipidemia, fatty liver, hyperglycemia, and hypertension. symptom.