54 results about "Alpha amylase inhibitor" patented technology

The invention relates to compositions comprising at least two inhibitors selected from the group consisting of an alpha amylase inhibitor, an alpha glucosidase inhibitor, and a sodium dependent glucose transporter inhibitor. The invention also provides methods of using the compositions for controlling glucose uptake.

The invention discloses a method for industrially extracting an alpha-amylase inhibitor in white kidney beans, belonging to the field of preparation of plant effective components. The method comprises the following steps: firstly adding water into soaked and peeled white kidney beans, grinding into slurry, sieving to remove residues, performing standing, centrifuging, enzyme deactivation, centrifuging and ethanol precipitation on an obtained filtrate to obtain protein, dissolving the obtained protein by using a phosphate buffer solution, performing moderate enzyme hydrolysis by using alkaline protease to change isoelectric points of impurity protein, then performing acid precipitation, collecting supernatant liquid, and performing ethanol precipitation and freeze drying to obtain an alpha-amylase inhibitor product. The prepared alpha-amylase inhibitor disclosed by the invention is high in purity and strong in activity; and moreover, the alpha-amylase inhibitor needs simple equipment, is low in cost, is pollution-free, has relatively good economic and environmental benefits, and can be applied to industrial large-scale production.

A white kidney bean extract extracted by microwave is disclosed, wherein the bioactivity of an alpha-amylase inhibitor contained in the extract is equal to or higher than 4100 U / mg, and the HPLC (high-performance liquid chromatography) content is equal to or higher than 20% w / w. Preparation method for the extract is characterized by comprising the following steps of: crushing white kidney beans and passing through a 60-mesh sieve; preparing a mixture which is uniformly mixed by the crushed and sieved white kidney bean powder and purified water in a weight ratio of 1:5, extracting for 1-10 minutes at a temperature below 40 DEG C in microwave extraction equipment, then filtering to obtain a filtrate, and removing the filter residue; heating the filtrate to 70 DEG C in a water bath with stirring, keeping for 20 minutes, and collecting an effluent through a silica gel column; and drying the effluent to prepare the white kidney bean extract. The extract can be used for preparing an appetite inhibitor. The preparation method disclosed by the invention shortens extraction time and effectively increases extraction rate. The purified water is used as an extraction solvent, as well as is safe and environment-friendly.

The invention discloses an industrial preparation method of a safe and efficient white kidney bean alpha-amylase inhibitor and belongs to the field of preparation of active plant ingredients. The industrial preparation method of the safe and efficient white kidney bean alpha-amylase inhibitor comprises the following steps: firstly grinding white kidney beans into powder, extracting by alkaline water, then adjusting supernatant liquor prepared by centrifuging till the supernatant liquor is acidic, carrying out enzyme deactivation, alkali treatment and ultra-filtration to obtain a retention liquid, and drying the retention liquid to obtain the white kidney bean alpha-amylase inhibitor product free of phytolectins and trypsin inhibitors. The alpha-amylase inhibitor product prepared by the method is free of activity of phytolectins and trypsin inhibitors; the alpha-amylase inhibitor product is high in activity, so that the white kidney bean alpha-amylase inhibitor product is safe and efficient; meanwhile, the industrial preparation method of the safe and efficient white kidney bean alpha-amylase inhibitor is simple in process and low in cost, is applicable to industrial large-scale production, and has high economic and social benefits.

The invention discloses a method for industrially preparing a white kidney bean Alpha-amylase inhibitor, and belongs to the field of the plant active ingredient preparation. The method comprises the following steps: firstly peeling white kidney beans by a dry method, dry-smashing, sieving and preparing bean flour, and extracting the bean flour by using water, collecting slurry after centrifuging,after performing adequate enzymolysis on the slurry by using Beta-amylase, to obtain enzymatic hydrolysate, filtering the enzymatic hydrolysate by using a ceramic film, performing ultrafiltration on obtained filtrate by using 80-150 kDa of an ultrafiltration membrane, performing the ultrafiltration on penetrated liquid by using 15-30 kDa of the ultrafiltration membrane, spray-drying trapped fluid,to obtain the white kidney bean Alpha-amylase inhibitor. The prepared white kidney bean Alpha-amylase inhibitor product is high in Alpha-amylase inhibitor activity, no starch, and no phytolectin andtrypsin inhibitor activity, and has the reliable physiological efficacy and safety. In addition, the preparation technology is green and environmental, capable of adapting the requirements of the modern industrial production, and has the remarkable economic benefit and the social benefit.

The present invention provides a plant-derived carbohydrase inhibitor, wherein the inhibitor is effective for preventing or alleviating diabetes, or preventing obesity, and foods, drinks, and medicines containing the same. The present invention is accomplished by use of an alpha-amylase inhibitor or an alpha-glucosidase inhibitor as a carbohydrase inhibitor that is extracted from astringent skins of a chestnut using ethanol or aqueous ethanol solution. The present invention can also be accomplished by adding the carbohydrase inhibitor to a food or medical composition as an active ingredient for delaying saccharide digestion or absorption, suppressing a rise in postprandial blood glucose levels or blood insulin levels, or preventing obesity.

This invention relates to the combination of α-amylase inhibitors prepared from Phaseolus vulgaris with α-glucosidase inhibitors obtained from Salacia oblonga and other species. The α-amylase inhibitor is accompanied by a quantity of lectins that reduces the amount of glucose originating from the starches present in the diet, and considerably reduces the appetite after repeated administration. The combination with α-glucosidase inhibitors, such as extracts of Salacia or the thiosugars present in it, further reduce the blood glucose, acting synergically, and consequently reduce the synthesis of fats from carbohydrates.

The present invention provides a phaseolus vulgaris alpha-amylase inhibitor extraction separation method, and relates to the field of food science biological active component extraction and separation, wherein the obtaining rate can be increased, and the yield can be improved. The method comprises: crushing phaseolus vulgaris to obtain phaseolus vulgaris crude powder, carrying out stirring extraction on the phaseolus vulgaris crude powder 3 times with water at a room temperature, filtering to obtain a filtrate, carrying out pressure reducing concentration on the filtrate to obtain a concentrated solution, carrying out boiling sterilization on the concentrated solution, adding a glucono delta-lactone coagulant, precipitating, filtering to obtain a precipitate, and sequentially carrying out decolorizing, dewatering, filtration draining, vacuum drying, and crushing so as to obtain the phaseolus vulgaris alpha-amylase inhibitor product.

The invention discloses a method for preparing an alpha-amylase inhibitor by an enzymatic method. In the method, white kidney beans are used as raw materials and are subjected to soaking, grinding and centrifugating; supernatant fluid is processed by steps by complex enzyme and is subjected to ion-exchange chromatography and hyperfiltration to prepare the alpha-amylase inhibitor. In the method, the white kidney beans are used as raw materials and the alpha-amylase inhibitor is prepared by the enzymatic method, therefore, the alpha-amylase inhibitor contained in the white kidney beans is effectively released to conduce to extraction and purification, thus reducing extraction cost. The method has mild reaction conditions, high purity of obtained products, good effect on inhibiting the amylase and inhibition activity as high as 70-95%, and can be widely applied in such fields as medicines, health-care food and the like.

The invention provides a compound preparation for reducing blood glucose, body weight and fat for human, and belongs to the technical field of biological medicines. The compound preparation comprises the following necessary active components in parts by weight: 0.002-0.5 part of kidney bean alpha-amylase inhibitor, 0.001-0.03 part of kidney bean phytagglutinin and 0.1-0.4 part of merremia hungaiensis or aqueous extract from merremia hungaiensis with the same part, and also comprises one or more of the following optional active components in parts by weight: 0.05-0.2 part of tremella polysaccharide, 0.1-0.3 part of hawthorn or aqueous extract from hawthorn with the same part, 0.1-0.2 part of cassia seed or aqueous extract from cassia seed with the same part, 0.1-0.2 part of salviae miltiorrhizae or aqueous extract from salviae miltiorrhizae with the same part, 0.1-0.2 part of astragalus membranaceus or aqueous extract from astragalus membranaceus with the same part and 0.1-0.3 part of dietary fibers or aqueous extract from dietary fibers with the same part. The compound preparation is a pure natural raw material combined preparation, has the effects of reducing blood glucose, body weight, fat and blood pressure at the same time.

The invention discloses an alpha-amylase inhibitor producing strain, i.e. Actinoplanes sp.ZJB-08196 preserved in the China Center for Type Culture Collection located in Wuhan university, Wuhan Province, China on Feb. 16th, 2009 with the preservation number of CCTCC No. M209022. The alpha-amylase inhibitor is acarbose. The invention also discloses a screening method of the alpha-amylase inhibitor producing strain. The screening method provided by the invention is simple and effective, can screen a great amount of samples in a short time, has high sensitivity, reduces the long processes for processing and analyzing samples through HPLC (High-Performance Liquid Chromatography), and the like and improves detection efficiency.

The invention features the treatment of gastrointestinal disorders associated with an innate immune response tiggered by alpha amylase inhibitor CM3, alpha amylase inhibitor 0.19 (0.19), CM1, CM2, CMa, CMd, CM16, CMb, CMX1 / CMX3, CMX2, and / or alpha amylase inhibitor 0.53 (0.53). To this end, the invention features pharmaceutical compositions including neutralizing antibodies to CM3, 0.19, CM1, CM2, CMa, CMd, CM16, CMb, CMX1 / CMX3, CMX2, and / or 0.53, food products containing reduced levels of CM3, 0.19, CM1, CM2, CMa, CMd, CM16, CMb, CMX1 / CMX3, CMX2, and / or 0.53 protein, the use of oral TLR4 inhibitors to block the effect of said alpha-amylase inhibitors, assays for identifying CM3, 0.19, CM1, CM2, CMa, CMd, CM16, CMb, CMX1 / CMX3, CMX2, and / or 0.53 content in food products, and assays for diagnosing subjects with a disorder related to CM3, 0.19, CM1, CM2, CMa, CMd, CM16, CMb, CMX1 / CMX3, CMX2, and / or 0.53 triggered innate immune responses.

The invention discloses a method for extracting an alpha-amylase inhibitor from hulless oats. The method comprises the following steps of: decladding and pulverizing a hulless oat seed; degreasing with acetone; and then carrying out ammonium sulfate precipitation, ion-exchange chromatography and gel chromatography to obtain an electrophoretic pure alpha-amylase inhibitor, wherein the inhibiting specific activity is 26.43U / mg. A material for extracting the alpha-amylase inhibitor is derived from the hulless oat, a purifying method is simple and convenient, a product has high purity and high inhibiting activity, and the extracted alpha-amylase inhibitor can be used for preparing a medicament for reducing weight and treating diabetes mellitus.

The invention discloses a double-water phase extracting system and a method of separating and purifying alpha-amylase inhibitor by it. And its phase forming reagents includes polyglycol and fructose-1, 6-diphosphoric metal salt that have stabilizing and protecting effect on the bioactivity of biochemical product. It applies the double-water phase system to the process of separate-extracting alpha-amylase inhibitor, uses the advantages of the double-water phase system: fast to separate phase, simple and convenient to operate and easy to amplify.

The invention discloses a method of extracting Alpha amylase inhibitor from kidney beans. The method is as follows: the crushed kidney beans are first dispersed in a buffer solution with a definite pH value, and then shifted into an ultrasonic device for ultrasonic extraction, and then conducted enzyme elimination, centrifugalization, dialysis and ethanol sediment, dried and finally the Alpha amylase inhibitor is obtained. The Alpha amylase inhibitor extracted from kidney beans in the invention is a white solid powder with a protein content of 80 to 95 percent and an inhibitory activity of 5,000 to 50,000 U / g. Compared with the prior method of lixiviation and extraction, the method of extracting Alpha amylase inhibitor from kidney beans has the characteristics of high speed, high efficiency, energy conservation and small pollution. The one-time extracted Alpha amylase inhibitor has high content and strong activity, thereby avoiding the fact that circulative extractions consume a large quantity of water and energy. The method of extracting Alpha amylase inhibitor from kidney beans is a novel green environment-friendly technology with a wide range of application prospects.

The invention provides a dietary substance supplementing composition, chewable tablets and a preparation method and application of the chewable tablets, and belongs to the technical field of dietary substance supplements. The dietary substance supplementing composition is prepared from, by mass, 20-40 parts of a white kidney bean extract, 15-35 parts of sorbitol, 10-25 parts of resistant dextrin and 1-3 parts of L-arabinose; the white kidney bean extract comprises an alpha-amylase inhibitor, and the mass percentage of the alpha-amylase inhibitor is greater than or equal to 55%. The dietary substance supplementing composition has the functions of reducing the postprandial blood glucose and managing the weight.

This invention relates to the combination of α-amylase inhibitors prepared from Phaseolus vulgaris with α-glucosidase inhibitors obtained from Salacia oblonga and other species. The α-amylase inhibitor is accompanied by a quantity of lectins that reduces the amount of glucose originating from the starches present in the diet, and considerably reduces the appetite after repeated administration. The combination with α-glucosidase inhibitors, such as extracts of Salacia or the thiosugars present in it, further reduce the blood glucose, acting synergically, and consequently reduce the synthesis of fats from carbohydrates.

The invention relates to a special medical food applicable to diabetics and a production technique thereof. The special medical food applicable to diabetics comprises the following components in parts by weight: 10-20 parts of dietary fiber block, 7-15 parts of algae, 5-15 parts of shredded orange peel, 10-20 parts of peanut kernel, 10-20 parts of walnut kernel, 5-10 parts of white kidney bean extract, 4-12 parts of tuckahoe, 4-10 parts of Lycium barbarum, 4-10 parts of semen dolichoris album, 6-12 parts of red bean, 20-40 parts of grain, 10-20 parts of pumpkin, 3-10 parts of chitin, 2-7 parts of concentrated whey protein, 4-12 parts of Polygonatum sibiricum, 4-12 parts of radix polygonati officinalis, 1-3 parts of Spirulina, 1-3 parts of cereus, 10-15 parts of balsam pear, 0.1-0.3 part of tea polyphenol, 0.05-0.1 part of zinc gluconate, 0.5-2 parts of milk calcium, 0.5-1 part of magnesium carbonate and 0.5-2 parts of sodium erythorbate. The special medical food applicable to diabetics has abundant nutrition, and is especially suitable for diabetics; and when the food is eaten before meals, the added alpha-amylase inhibitor can prevent calorie intake of most starch without influencing the absorption of other nutrient substances, thereby reducing the maximal fat source of the human body.

The present invention discloses one kind of solid mixture containing alpha-amylase and its preparation process and application in preparing medicine. The solid mixture is prepared through preparing coarse extract of alpha-amylase inhibitor via conventional process; adding sugar alcohol in the weight ratio to the coarse extract of alpha-amylase inhibitor organic 0.1-99.9 % to the coarse extract of alpha-amylase inhibitor via stirring and drying to obtain the mixture. The mixture may be used in preparing medicine for preventing and treating obesity, lipomatosis, atherosclerosis, hyperlipemia and diabetes. The mixture can maintain the bioactivity of alpha-amylase inhibitor with less moisture absorption and high stability. The preparation process is simple and easy use in industrial production.

A nutritional supplement composition contains inhibitors of alpha-glucosidase and alpha-amylase substantially in the absence of lipase inhibitors. The composition can include touchi extract and phaseolamin as the alpha-glucosidase and alpha-amylase inhibitor, respectively. A method of limiting the absorption of carbohydrates contained in a foodstuff includes administering the nutritional supplement composition prior to consumption of the carbohydrates, and a method of promoting weight loss in an individual includes administering the nutritional supplement composition to the individual over a period of days.

The invention provides a method for preparing deproteinized rice and a product and for jointly preparing an alpha-amylase inhibitor and a protein peptide. The preparation method specifically comprisesthe following steps: washed rice or rice flour is used as a raw material; after sterilization, first enzymolysis is carried out by using an enzymatic hydrolysate to prepare alpha-amylase inhibitory peptide; after a solid material obtained by the first enzymolysis is sterilized, second enzymolysis is conducted and a second enzymatic hydrolysate is used to prepare protein peptide; a solid materialobtained by the second enzymolysis is directly steamed, dried and dispersed to obtain deproteinized rice or rice flour containing resistant starch, or is used to prepare box lunch; specific protease is added during the first enzymolysis, or zymophyte and specific protease are simultaneously added to perform enzymolysis and fermentation; and non-specific protease and triglyceride lipase are added during the second enzymolysis. According to the invention, contents of protein, sodium, potassium and phosphorus in rice are reduced, the alpha-amylase inhibitor and the antioxidant and ACEI-activity protein peptide are jointly prepared, and the content of resistant starch in deproteinized rice is increased.

The invention belongs to the technical field of medicine. The invention relates to application of tetrahydro isoquinoline alkaloid which is shown in a formula (I) or a formula (II) or a pharmaceutically acceptable salt of the tetrahydro isoquinoline alkaloid in an aspect of preparing alpha-amylase inhibitors. Activity screening experiments prove that the compound disclosed by the invention has an effect of inhibiting activity of two kinds of in-vitro digestive enzyme of alpha-amylase and pancreatic lipase and inhibiting proliferation and differentiation activity of 3T3-L1 preadipocyte at the same time and has an application prospect in an aspect of clinical research on diabetes, hyperlipemia, obesity or metabolic syndrome.

The invention discloses a production and purification method of a storage protein type alpha-amylase inhibitor with a Cupin structure function domain, comprising the steps of: 1, construction of a rokaryotic expression system and bacterium solution cultivation inducible expression; 2, prokaryotic expression of soluble protein purification; and / or 3, prokaryotic expression of inclusion body protein purification and protein renaturation. At present, multiple repeated experiments test that, the protein obtained through producing the alpha-amylase inhibitor by using the technical process provided by the invention is high in yield and purity, the production process is simple and convenient, the production cycle is short, expensive devices are not needed, and thereby the production and purification method is an excellent bioprocess technology for producing and purifying the alpha-amylase inhibitor.

The invention discloses a method for screening an alpha-amylase inhibitor. The method comprises: preparing an enzymatic reaction buffer solution, a substrate solution, an alpha-amylase solution, a color-developing reagent solution, and a to-be-tested product solution for later use; performing sample application of the to-be-tested product solution on a thin layer plate; allowing a solvent to be volatilized or using a proper developing solvent for developing, performing air-drying, and soaking the obtained product in the substrate solution; taking the obtained product out, allowing the solvent to be volatilized, soaking the obtained product in the alpha-amylase solution, and carrying out an enzymatic reaction at 5-50 DEG C for 10-60 min; after the reaction is completed, taking the obtained thin layer plate out, allowing the solvent to be volatilized, soaking the obtained product in the color-developing reagent solution for a color-developing reaction; and viewing the obtained thin layer plate in visible light. Blue spots which appear in the white or yellow or brown background are the alpha-amylase inhibitor. The invention provides the method which enables the alpha-amylase inhibitor to be conveniently fast screened and provides an ideal approach for screening active ingredients containing the alpha-amylase inhibitor from natural substances.

The invention discloses the application of an alpha-amylase inhibitor in preparing a medicine for preventing and curing hyperlipidemia and atherosclerosis, where the medicine can prevent and cure hyperlipidemia and atherosclerosis, safe, nontoxic, having good endurance and especially suitable to be applied long term.

The invention discloses a method for efficiently extracting a mung bean alpha-amylase inhibitor. According to the method, the alpha-amylase inhibitor with uniform ingredients is extracted and purified from mung bean by adopting gel column chromatography and ethanol precipitation, meanwhile, the influence of mung bean alpha-amylase inhibitor extraction conditions on the inhibitory activity of Porcine pancreatic alpha-amylase is analyzed, and the best extraction process conditions of the mung bean alpha-amylase inhibitor are determined. The mung bean alpha-amylase inhibitor extracted according to the method disclosed by the invention has high temperature stability and accordingly has a broad application prospect in the development of safe and natural blood glucose reducing drugs.

A new quasi-oligosaccharide alpha-amylase inhibitor as alcaftadine compound is represented as the formula (I) at right, wherein m is integer of 0-7, n is integer of 1-4 and q is integer of 0-3, The mixture and monomer of the alcaftadine compound are white amorphous powder which dissolves in water and DMSO easily, dissolves in methanol, alcohol and acetone slightly and not dissolves in other organic solvents. The alcaftadine compound contains different amounts of acarviosine-amylaceum structural units and reducing glucose terminals, particularly the compound with high repeated times and significant different structure with prior quasi-oligosaccharide alpha-amylase inhibitor. Therefore, the compound is a new quasi-oligosaccharide alpha-amylase inhibitor with the application for preparing the drug of diabetes and adiposis or the like.

The invention discloses a method for determining the activity of an alpha-amylase inhibitor by a colorimetric method via resin de-coloring. According to the method, the interferences on the colorimetric analysis and detection of the activity of alpha-amylase by pigments of a traditional Chinese medicine extracting solution can be effectively reduced; a method which has the advantages of simplicity, practicability, environmental protection and good analysis repeatability is provided for screening the alpha-amylase inhibitor in vitro. The method comprises the following steps: crushing plants or medicinal materials; extracting by water or alcohol; combining filtering solutions and centrifuging a certain quantity of filtering solution; after centrifuging, taking a liquid supernatant; enabling the liquid supernatant taking as sampling liquid to pass through a D101 macroporous resin column; carrying out gradient eluting by using ethanol-water solutions with different concentrations; and respectively collecting flow parts with different gradients to obtain the flow parts which have different color degrees and have different activities on the alpha-amylase inhibitor. According to the method, the D101 macroporous resin column is used for de-coloring a medicinal material extracting solution so as to relieve the interferences on the colorimetric analysis by a color of the inhibitor in the process of determining the activity of the alpha-amylase inhibitor.

The invention discloses a method for preparing a highly-pure Porphyra yezoensis polysaccharide alpha-amylase inhibitor, and belongs to the field of preparation of effective components in plants. The method comprises the following steps: milling Porphyra yezoensis, sieving the milled Porphyra yezoensis, performing water extraction, adding a supernatant obtained by centrifugation into protease to carry out enzymatic hydrolysis, performing enzyme deactivation, performing ultrafiltration on a supernatant obtained by centrifugation, and performing spray drying on the obtained retentate to obtain the Porphyra yezoensis polysaccharide alpha-amylase inhibitor product. The alpha-amylase inhibitor prepared in the invention has the advantages of non-toxicity, high purity, high vitality, good safety,high efficiency, simple process, low cost and good economic and social benefits.

The invention discloses a method for specifically removing dimerization alpha-amylase inhibitor and avenin-like substances from yellow rice wine and belongs to the field of production technologies ofthe yellow rice wine. The method comprises the following steps: adding green persimmon tannin into wine liquid blended with Shaoxing yellow rice wine, specifically adsorbing yellow rice wine turbidity-sensitive proteins, namely the dimerization alpha-amylase inhibitor and avenin-like substances, separating out the generated compounds from the wine liquid at low temperature, forming precipitates, and removing by adopting a filtration method. Protein stability of bottled Shaoxing yellow rice wine treated by green persimmon tannin is obviously improved, the turbidity in a quality guarantee periodis always lower than 1.5EBC, no obvious macroscopic turbid matter is formed, quality of the yellow rice wine is improved, and certain economic benefit is brought to an enterprise.