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Method for preparing alpha-amylase inhibitor by enzymatic method

The technology of an amylase inhibitor and a biological enzyme method is applied in the field of preparation of amylase inhibitors, and can solve the problem of high alpha-amylase inhibitor activity and achieve the effects of good inhibitory effect, favorable extraction and purification, and high purity

Inactive Publication Date: 2009-10-14
GUANGDONG FOOD IND INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, α-amylase inhibitors have been isolated from seeds of wheat, barley, sorghum, cereals, peanuts, soybeans, taro, wild amaranth, etc., but the activity of α-amylase inhibitors extracted from white kidney beans is relatively high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Weigh 100 g of white kidney beans, add water at a ratio of 1:10, soak for 12 hours, and crush. Centrifuge the obtained material, adjust the pH value of the supernatant to 4.5±0.1, raise the temperature to 45°C, add 0.2% glucoamylase to react for 1 hour, and raise the temperature to 70°C to inactivate the enzyme. After the temperature drops to room temperature, adjust the pH value to 6.5±0.1, then raise the temperature to 50°C, add 0.1% lipase to react for 1 hour, and then raise the temperature to 70°C to inactivate the enzyme. The solution after deactivating the enzyme passes through the strongly acidic styrene-based cation exchange resin at a flow rate of 2mL / min, and the solution after the resin treatment is kept at 40°C at a relative flow rate of 1.5mL / min / cm 2 Through the roll-type ultrafiltration membrane, the product after membrane filtration is concentrated and dried to obtain the α-amylase inhibitor product. The inhibitory activity of the product is about 90%. ...

Embodiment 2

[0025] Weigh 100 g of white kidney beans, add water at a ratio of 1:10, soak for 24 hours, and crush. Centrifuge the obtained material, adjust the pH value of the supernatant to 4.8±0.1, raise the temperature to 45°C, add 0.5% glucoamylase to react for 4 hours, and raise the temperature to 70°C to inactivate the enzyme. After the temperature drops to room temperature, adjust the pH value to 6.3±0.1, then raise the temperature to 50°C, add 0.2% lipase to react for 1 hour, and then raise the temperature to 70°C to inactivate the enzyme. The solution after deactivating the enzyme passes through the strongly acidic styrene-based cation exchange resin at a flow rate of 2mL / min, and the solution after the resin treatment is kept at 40°C at a relative flow rate of 1.5mL / min / cm 2 Through the roll-type ultrafiltration membrane, the product after membrane filtration is concentrated and dried to obtain the α-amylase inhibitor product. The inhibitory activity of the product is about 87%....

Embodiment 3

[0027] Weigh 100 g of white kidney beans, add water according to the ratio of 1:7, soak for 8 hours, and crush. Centrifuge the obtained material, adjust the pH value of the supernatant to 4.3±0.1, raise the temperature to 45°C, add 0.1% glucoamylase to react for 4 hours, and raise the temperature to 60°C to inactivate the enzyme. After the temperature drops to room temperature, adjust the pH value to 6.3±0.1, then raise the temperature to 50°C, add 0.1% lipase to react for 1 hour, and then raise the temperature to 60°C to inactivate the enzyme. The solution after deactivating the enzyme passes through the strongly acidic styrene-based cation exchange resin at a flow rate of 1mL / min, and the solution after the resin treatment is kept at 40°C at a relative flow rate of 1mL / min / cm 2 Through the roll-type ultrafiltration membrane, the product after membrane filtration is concentrated and dried to obtain the α-amylase inhibitor product. The inhibitory activity of the product is ab...

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Abstract

The invention discloses a method for preparing an alpha-amylase inhibitor by an enzymatic method. In the method, white kidney beans are used as raw materials and are subjected to soaking, grinding and centrifugating; supernatant fluid is processed by steps by complex enzyme and is subjected to ion-exchange chromatography and hyperfiltration to prepare the alpha-amylase inhibitor. In the method, the white kidney beans are used as raw materials and the alpha-amylase inhibitor is prepared by the enzymatic method, therefore, the alpha-amylase inhibitor contained in the white kidney beans is effectively released to conduce to extraction and purification, thus reducing extraction cost. The method has mild reaction conditions, high purity of obtained products, good effect on inhibiting the amylase and inhibition activity as high as 70-95%, and can be widely applied in such fields as medicines, health-care food and the like.

Description

technical field [0001] The invention relates to a preparation method of an amylase inhibitor. Background technique [0002] At present, the number of obese patients in the world is increasing at an alarming rate. Experts predict that at the current rate of development, all Americans will become obese patients by 2230; while in the UK, in the past 20 years, the number of overweight and obese men has increased by 15% rose to 54%, and women rose to 45%. Even in a developing country like China, obesity has become a serious public health problem in China. At present, there are far more than 90 million obese people in my country, and 200 million overweight people. Experts predict that in the next ten years, the number of obese people in China will exceed 200 million. Moreover, obesity will not only lead to diabetes, high blood pressure, cancer and many other diseases, but also cause premature death. Data show that the risk of premature death of obese people is 1.3 to 2 times t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K1/14
Inventor 寇秀颖徐勇王欣骆海平
Owner GUANGDONG FOOD IND INST
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