11results about How to "Reduce extraction costs" patented technology

This invention discloses a primer and probe set, a kit, and their applications for detecting Staphylococcus aureus L based on real-time fluorescence MIRA technology. The primer and probe set consists of single-stranded DNA sequences 1-3. Based on the aforementioned primer and probe set, this invention establishes and optimizes a real-time fluorescence MIRA detection method for Staphylococcus aureus L. Furthermore, the optimized real-time fluorescence MIRA method is innovatively combined with a simplified nucleic acid extraction method and a portable fluorescence detection device, significantly reducing extraction and time costs while enabling rapid on-site detection of the virus, making it particularly suitable for grassroots units and remote areas. In addition, the real-time fluorescence MIRA detection method for Staphylococcus aureus L established in this invention has advantages such as high specificity, high sensitivity, and simple operation. Moreover, the detection process does not require opening the lid, effectively avoiding aerosol contamination, providing an effective technical means for the differential diagnosis and rapid detection of Staphylococcus aureus L.

This invention relates to the field of natural product separation and recombination, and particularly to a recombinant saponin from *Gleditsia sinensis* and its preparation method. The method includes: 1) crude saponin extraction: water extraction of *Gleditsia sinensis* pericarp to obtain a crude saponin solution; 2) impurity removal: loading the crude saponin aqueous solution into macroporous adsorption resin AB-8 and eluting to remove impurities; 3) saponin component separation: eluting sequentially with 25-35% ethanol solution, 40-50% ethanol solution, 55-65% ethanol solution, and 70-80% ethanol solution to obtain S30, S45, S60, and S75 saponin components, respectively; 4) saponin hydrolysis: hydrolyzing the S45 saponin component to obtain hydrolyzed saponin; 5) saponin component recombination: recombining the saponin components obtained in steps 3) and 4) with the hydrolyzed saponin to obtain recombinant saponin from *Gleditsia sinensis*. This invention enables saponin purification and improves the surface activity of *Gleditsia sinensis*.

The invention provides a clay type lithium ore external field enhanced resource comprehensive utilization method, and relates to the field of hydrometallurgy. The method comprises the following steps: uniformly mixing clay type lithium ore with concentrated sulfuric acid, and carrying out low-temperature curing to obtain clinker; mixing the clinker with water, and then carrying out leaching reaction to obtain a lithium-rich aluminum leaching solution; carrying out evaporative crystallization on the lithium-rich aluminum leachate to obtain a mixed crystal of lithium sulfate and aluminum sulfate; performing high-temperature pyrolysis reaction on the mixed crystal to obtain an oxide; carrying out high-energy grinding and leaching on the oxide, and filtering to obtain a lithium-containing solution and crude aluminum oxide; and carrying out post-treatment on the lithium-containing solution to obtain the battery-grade lithium carbonate. According to the method, the technological process of the clay type lithium ore is optimized, synchronous leaching of valuable elements lithium and aluminum and efficient separation of the lithium element and the aluminum element are achieved, the problem that the cost is high when lithium is independently extracted is solved, cooperative utilization of clay type lithium ore resources is achieved, and the cost is reduced.

This disclosure provides a method, apparatus, device, and storage medium for acquiring and applying an operations and maintenance (O&M) knowledge graph, which can be applied to the field of O&M service technology. The method includes: acquiring O&M resource data from a device information resource database; performing structured identification on the O&M resource data to obtain static O&M knowledge, which includes multiple first entities; acquiring real-time O&M service work order data; using a pre-trained knowledge extraction model to perform unstructured identification on the O&M service work order data to obtain dynamic O&M knowledge, which includes multiple second entities; acquiring historical relationships between the multiple first entities and the multiple second entities; inferring new relationships corresponding to the first entities in the O&M service work order data based on the first similarity between the multiple first entities, the second similarity between the multiple second entities, and the historical relationships; and obtaining an O&M knowledge graph based on the new relationships.

The present application belongs to the technical field of extraction and utilization of high-value components in industrial tail gas, and discloses a device and method for extracting hydrogen from industrial tail gas. The device comprises a heat exchanger, a high-temperature-resistant ceramic cylinder, a direct-current power supply and a cooler. Water vapor is used as a carrier gas to carry out the prepared hydrogen, which can reduce the electrolysis voltage and the power consumption for extracting hydrogen. The method of applying an external voltage is used to provide the driving force for hydrogen extraction, which can extract hydrogen from tail gas with low hydrogen concentration and improve the utilization rate of hydrogen resources. The device and method for extracting hydrogen from low-hydrogen-content high-temperature industrial tail gas are provided to solve the problem that hydrogen cannot be directly extracted from high-temperature industrial tail gas with low hydrogen concentration, resulting in a large amount of hydrogen resource waste. The device and method can directly extract hydrogen from high-temperature industrial tail gas with low hydrogen content by using high-temperature proton conductor ceramics, and water vapor is used as a carrier gas to carry the extracted hydrogen, which can reduce the power consumption for extracting hydrogen and efficiently prepare high-purity hydrogen.

This invention relates to the field of natural saponin separation and recombination, and particularly to a complex of saponin from *Gleditsia sinensis* and saponin from *Camellia oleifera* and its preparation method. The method includes: 1) extracting saponin from *Gleditsia sinensis* pericarp and *Camellia oleifera* cake, respectively, to obtain crude saponin solutions from *Gleditsia sinensis* and crude saponin from *Camellia oleifera*; 2) using macroporous adsorption resin AB-8 as a resin separation column to elute and remove impurities from the crude saponin solutions from *Gleditsia sinensis* and crude saponin from *Camellia oleifera*; 3) eluting the obtained saponin solutions from *Gleditsia sinensis* and *Camellia oleifera* with 25-35% ethanol solution, 40-50% ethanol solution, 55-65% ethanol solution, and 70-85% ethanol solution, respectively, to obtain saponin components from *Gleditsia sinensis* and saponin components from *Camellia oleifera*; 4) recombining the saponin components from *Gleditsia sinensis* and *Camellia oleifera* to obtain the complex of saponin from *Gleditsia sinensis* and *Camellia oleifera*. This invention can improve the purity and surface activity of natural saponins, overcoming the disadvantage of the high critical micelle concentration of *Camellia oleifera*.

The invention discloses a rectifying still residue extraction method, and belongs to the field of environmental protection and the technical field of hazardous waste harmless resource utilization. The technical problem that the recovery rate of residual NMP in the current rectifying still residues is not high is solved. The method comprises the following steps: soaking the residual lignosulfonate in an NMP rectifying still, adding an organic solvent for treatment, and then rectifying to obtain NMP. According to the method, the NMP is extracted by utilizing the boiling point difference between the organic solvent and the NMP rectifying still residue and temperature-controlled rectification, and the lignosulfonate is used as a water reducing agent to be compounded with water, so that the moisture required when the solid still residue is dissolved is reduced, and the flowability of the still residue after the organic solvent is added is improved. In addition, the organic solvent is matched with water and NMP (N-Methyl Pyrrolidone) in rectifying still residues, so that the azeotropic phenomenon is reduced. According to the method, the NMP rectifying still residues which cannot be recycled are pretreated with chemical reagents such as diethylene glycol and then rectified, so that a large amount of NMP is recovered, and the utilization efficiency of NMP is greatly improved.

The application relates to the field of biochemical technology, and particularly discloses a method for efficiently extracting green mung bean starch. The method comprises the following steps: obtaining green bean sprouts by ultrasonic-assisted soaking and germination culture of green mung beans; obtaining green bean sprout powder by drying, crushing and sieving the green bean sprouts; mixing the green bean sprout powder with a NaOH solution, oscillating, washing, centrifuging, filtering and obtaining pure starch, and finally drying, grinding and sieving to obtain germinated green mung bean starch. The extraction method combines ultrasonic pretreatment with a germination process, effectively improves the gelatinization characteristics of the starch, significantly improves the starch viscosity, thereby enhancing the applicability of the starch in food processing, and meanwhile, the nutritional and functional characteristics of the green mung bean starch are retained.

The application specifically discloses a method for recovering nickel, cobalt and manganese from a material containing nickel, cobalt and manganese, which comprises the following steps: a first leaching process, a pH adjusting process, a first solid-liquid separation process, a first impurity removal process, a second leaching process and a second impurity removal process. The method for recovering nickel, cobalt and manganese from the material containing nickel, cobalt and manganese provided by the application does not need to add oxidants and reducing agents, the pH value of the first mixed slurry obtained in the first leaching process is adjusted by adding alkali, the pressure filtration time of liquid-solid separation can be effectively reduced, the amount of auxiliary materials used in the subsequent impurity removal process can also be reduced, the solid manganese concentrate obtained after the second leaching process can be washed simply, the manganese concentrate can be directly sold, the subsequent manganese extraction process is omitted, the extraction cost of manganese is reduced, and the economic benefit is further improved. Therefore, the recovery method provided by the application has the characteristics of low cost and good economic benefit.

This invention discloses a sealing and recovery device and method for a multi-channel gas extraction pipe, belonging to the field of gas extraction device and recovery technology. The device includes a bladder, a multi-channel gas extraction pipe, a protective pipe, and a recovery device. The multi-channel gas extraction pipe has a cross-shaped partition inside, forming four channels: two gas extraction channels, a grouting material channel, and a liquid injection channel. The bladder has a cylindrical structure, with two bladders respectively fitted onto the outer walls of the multi-channel gas extraction pipe at both ends. The bladder contains several sets of buoyancy plates, each set connected in series by two traction ropes, with both ends of the traction ropes connected to the inner wall of the bladder. This invention integrates the grouting material pipeline, the gas extraction pipeline, and the liquid injection channel into one unit, achieving multiple uses with a single pipe and thus saving economic costs. After the grouting sealing material solidifies, the multi-channel gas extraction pipe and the bladder can be recovered, realizing the recovery and multiple uses of the gas extraction pipe and the bladder within the solidified sealing material.

Embodiments of the present application disclose an image information extraction method and device. The image information extraction method extracts information of a to-be-recognized image by calling a target basic extractor according to configuration information to determine each target text in the to-be-recognized image after obtaining the to-be-recognized image and the configuration information. The configuration information is used to represent a calling requirement for at least one target basic extractor, and each target basic extractor is used to extract a target text of a corresponding information type. The method can reduce the image information extraction cost and improve the image information extraction efficiency.