68results about How to "Reduce extraction costs" patented technology

This invention discloses a primer and probe set, a kit, and their applications for detecting Staphylococcus aureus L based on real-time fluorescence MIRA technology. The primer and probe set consists of single-stranded DNA sequences 1-3. Based on the aforementioned primer and probe set, this invention establishes and optimizes a real-time fluorescence MIRA detection method for Staphylococcus aureus L. Furthermore, the optimized real-time fluorescence MIRA method is innovatively combined with a simplified nucleic acid extraction method and a portable fluorescence detection device, significantly reducing extraction and time costs while enabling rapid on-site detection of the virus, making it particularly suitable for grassroots units and remote areas. In addition, the real-time fluorescence MIRA detection method for Staphylococcus aureus L established in this invention has advantages such as high specificity, high sensitivity, and simple operation. Moreover, the detection process does not require opening the lid, effectively avoiding aerosol contamination, providing an effective technical means for the differential diagnosis and rapid detection of Staphylococcus aureus L.

The invention discloses an extraction method of silymarin. The extraction method of the silymarin comprises the steps of polar solvent treatment, acid solution treatment, alkaline solution treatment and purification. The polar solvent is selected from one or more of chloroform and dichloromethane, and the alkaline solution comprises one or more of sodium hydroxide and potassium hydroxide. The extraction method of the silymarin provided by the invention has the effects of reducing the extraction difficulty and cost of the silymarin.

The invention discloses a method for separating and purifying squalene from squalene fermentation liquor, and belongs to the technical field of squalene extraction. The method comprises the following steps: 1) pretreatment: adding a wall breaking reagent into squalene fermentation liquor for wall breaking, or concentrating the squalene fermentation liquor and then adding the wall breaking reagent for wall breaking; 2) extraction: adding an organic solvent and a demulsifier, layering to obtain a water phase and an organic phase, and collecting the organic phase; 3) impurity removal: adding an aqueous solution, layering to obtain an aqueous phase and an organic phase, and collecting the organic phase; and 4) distilling: distilling to obtain purified squalene. The method is simple to operate, low in equipment investment, simple in post-treatment and low in extraction cost, and the obtained squalene refined product is high in yield, high in purity and suitable for industrialization.

The invention relates to the technical field of extraction and utilization of electrolytic aluminum lithium-containing waste, in particular to a method for extracting lithium from electrolytic aluminum waste through ultrasonic strengthening. The method comprises the following steps: (1) coarsely crushing the electrolytic aluminum waste; (2) after the carbon is removed, the electrolytic aluminum waste is subjected to ultrasonic strengthening to strengthen the leaching of lithium, and the lithium is selectively leached by controlling the leaching acidity; (3) performing two-stage impurity removal on the lithium leaching solution; and (4) concentrating and denitrifying the impurity-removed liquid, precipitating lithium, carbonizing and crystallizing to prepare the battery-grade lithium carbonate. According to the method, carbon in the electrolytic aluminum waste is removed through a calcination or combustion method, leaching of lithium is strengthened through an ultrasonic strengthening method, meanwhile, the leaching condition is controlled to enable lithium in the waste aluminum slag to be dissolved out into a leaching solution, and aluminum, iron, sodium and most fluorine in the waste aluminum slag are not dissolved out and left in the leaching slag. And performing simple impurity removal, concentration and denitration on the leachate to prepare the battery-grade lithium carbonate. The method is simple in process and high in lithium extraction rate, and cryolite is mainly contained in leaching residues and can be utilized subsequently.

The invention relates to the technical field of polysaccharide extraction, in particular to a preparation method of scenedesmus polysaccharide. The invention provides a preparation method of scenedesmus polysaccharide, the scenedesmus polysaccharide and application of the scenedesmus polysaccharide. According to the method disclosed by the invention, a compound enzyme-assisted enzymolysis treatment process is adopted, scenedesmus powder is taken as a raw material, and high extraction yield and purity of scenedesmus polysaccharide are kept by controlling process parameters such as different enzyme addition amounts and enzymolysis pH; a degreasing step is omitted, and meanwhile, the enzymolysis time is prolonged, so that a relatively good decolorizing effect is also ensured. Besides, anion exchange column chromatography and gel column chromatography are adopted, and the scenedesmus crude polysaccharide (TBP) is separated to obtain four components with high purity, namely TBP-1, TBP-2, TBP-3 and TBP-4. The preparation method of scenedesmus polysaccharide has the advantages of simple operation steps, low requirements on production equipment, environmental friendliness, low extraction cost and the like, and is suitable for large-scale industrial production.

The invention discloses a method for efficiently extracting high-purity chlorogenic acid and chlorogenic acid substances from stevia rebaudiana. The method comprises the following steps: concentrating stevia rebaudiana extract liquid to obtain permeate liquid I and trapped liquid I; adjusting the pH of the trapped fluid I to obtain a to-be-treated fluid; treating the liquid to be treated by adopting an electrodialysis-ultrafiltration coupling device, and collecting an ion phase I, an ion phase II and a desalted phase; adsorbing the ion phase II by adopting macroporous adsorption resin, and respectively analyzing by adopting a low-alcohol solution and a high-alcohol solution to obtain a low-alcohol analysis solution and a high-alcohol analysis solution; concentrating the high-alcohol desorption solution to obtain trapped fluid II, mixing and crystallizing the trapped fluid II and chlorogenic acid seed crystals, filtering to obtain filtrate I and a filter cake I, refining the filter cake I to obtain filtrate II and a filter cake II, and drying the filter cake II to obtain high-purity chlorogenic acid; concentrating the ion phase I to obtain trapped fluid III, and performing thermal concentration and spray drying on the trapped fluid III to obtain the chlorogenic acid substances. According to the method, the stevia rebaudiana is used as a raw material, high-content chlorogenic acid and chlorogenic acid substances are obtained, less waste liquid and waste materials are generated in the whole process, and the method is more environmentally friendly.

The invention provides bacterial cellulose modified in situ by utilizing Sanzan gum and a preparation method thereof, and belongs to the technical field of biopolymer materials and biological fermention.The preparation method comprises the following steps that yeast powder, peptone, Na2HPO4, KNO3 and glucose are mixed with water, then the Sanzan gum is slowly added and stirred until the Sanzan gum is completely dissolved, and a mixture is obtained; then adding vegetable oil and a fat-soluble modifier to prepare an HS-SG fermentation culture medium; inoculating the FY-07 seed solution into an HS-SG fermentation culture medium for culture and fermentation; fishing out a bacterial cellulose membrane generated by fermentation, washing with water, and removing thalli and a residual culture medium in the bacterial cellulose hydrate by using a NaOH solution under a high-temperature condition, so as to obtain a bacterial cellulose wet membrane. Therefore, the emulsification effect of the Sanzan gum can realize uniform dispersion of fat-soluble components, so that uniform in-situ modification of the bacterial cellulose is realized, and the in-situ modified bacterial cellulose with good oxidation resistance and good biocompatibility is further obtained.

The present application discloses a seaweed active ingredient and a gradient extraction process thereof, and belongs to the field of seaweed extraction technology. The gradient extraction process of the seaweed active ingredient comprises the following steps: harvesting freshly salvaged seaweed, washing impurities on its surface with seawater, then slicing and adding sodium carbonate solution to stir, to obtain seaweed slurry; adding ethanol thereto and stirring, and obtaining a precipitate and an ethanol extract 1 after extrusion; adding ethanol to the precipitate and stirring, and then centrifuging to obtain a crude alginate extract and a supernatant 1, which is evaporated to recover ethanol and obtain a crude fucoidan extract; adding water to the ethanol extract 1 and stirring, and then centrifuging to obtain a crude fucoxanthin extract and a supernatant 2, which is evaporated to recover ethanol and obtain a crude brown algae polyphenol extract. The gradient extraction process can obtain crude extracts of multiple active ingredients at the same time, and does not require the use of a large amount of fresh water resources and toxic reagents, does not generate waste, is simple to operate, and has low cost, which is conducive to promotion and application.

The invention discloses a preparation method and application of a kava extract with soothing and anti-allergy capacities, and the preparation method comprises the following steps: putting kava pepper root powder into an extraction tank, and carrying out subcritical extraction on the kava pepper root powder by using an organic solvent as an extraction solvent to obtain an extraction liquid; sequentially carrying out reduced pressure evaporation and filtration on the extract to obtain a crude extract; adding the crude extract into a feeding bottle of molecular distillation equipment, and carrying out molecular distillation treatment to obtain yellow oily liquid; and finally, drying the yellow oily liquid to obtain the kavalactone extract. The method has the characteristics of high extraction efficiency, no solvent residue and low preparation cost.

The invention provides an extraction method of molybdenum trioxide, and belongs to the technical field of extraction metallurgy. The extraction method of molybdenum trioxide provided by the invention comprises the following steps: carrying out vacuum melting on a molybdenum-containing material to obtain a sublimate, and cooling the sublimate to obtain molybdenum trioxide, the molybdenum content in the molybdenum-containing material is 37.42 to 46.31 wt%, and the oxygen content in the molybdenum-containing material is 30.74 to 38.13 wt%; the temperature of vacuum melting ranges from 880 DEG C to 1200 DEG C, and the vacuum degree ranges from 0.47 Pa to 10 Pa; the cooling rate of the cooling is 5-10 DEG C / min. According to the method, the molybdenum-containing material is subjected to vacuum melting, molybdate in the molybdenum-containing material is decomposed to obtain molybdenum trioxide, original molybdenum trioxide of the molybdenum-containing material is sublimated under the high-temperature condition, high-purity molybdenum trioxide is obtained through cooling, and the extraction efficiency of molybdenum trioxide is effectively improved; and the extraction method is simple, the low-grade molybdenum-containing material is used as the raw material, the extraction cost is lower, and no pollution is caused to the environment.

The present invention belongs to the technical field of separation of natural active ingredients, and specifically relates to a method and application of extracting epicatechin using ionic liquid. The preparation method comprises the following steps: (1) mixing tea residues and ionic liquids evenly, performing extraction reaction, and then filtering to remove impurities to obtain tea residue extract; (2) applying the tea residue extract to a column, separating and eluting by preparative column chromatography, collecting the fraction containing epicatechin according to the position of the chromatographic peak, and then freeze-drying to obtain epicatechin; the ionic liquid comprises 1-butyl-3-methylimidazole chloride and / or 1-ethyl-3-methylimidazole chloride. The present invention extracts tea residues under specific conditions by using specific ionic liquids, and can extract epicatechin from tea residues with extremely high efficiency, and the extraction performance is significantly improved compared with traditional reagents, and the operation is convenient, time-saving, and cost-effective, and it is easy to industrialize production, suitable for a wide range of application fields and has huge market value.

The present invention belongs to the technical field of flue gas desulfurization wastewater treatment, and specifically relates to a flue gas desulfurization wastewater treatment device, including a distillation box, wherein the distillation box is provided with two crushing rollers, the distillation box is provided with a guide plate, the left and right ends of the two crushing rollers penetrate the distillation box and are provided with driven gears, the outer sides of the left and right driven gears of the crushing rollers located on the rear side are fixedly connected with intermediate gears, a horizontal shaft rotatably connected to the distillation box is provided on the lower side of the intermediate gear, driving gears are provided on the left and right ends of the horizontal shaft, the transmission ratio of the driving gear to the intermediate gear is 7:1, a plurality of wind cups are fixedly connected to the side surfaces of the left and right ends of the horizontal shaft, the left and right ends of the distillation box are connected with metal air pipes, the metal air pipes are provided with high-pressure conduction valves, and the end of the metal air pipes is provided with blowing nozzles matching the wind cups; the treatment device can distill and dehydrate solid precipitates in the flue gas desulfurization wastewater, and can also crush solid precipitates with larger volumes in the wastewater.

This invention relates to the field of natural product separation and recombination, and particularly to a recombinant saponin from *Gleditsia sinensis* and its preparation method. The method includes: 1) crude saponin extraction: water extraction of *Gleditsia sinensis* pericarp to obtain a crude saponin solution; 2) impurity removal: loading the crude saponin aqueous solution into macroporous adsorption resin AB-8 and eluting to remove impurities; 3) saponin component separation: eluting sequentially with 25-35% ethanol solution, 40-50% ethanol solution, 55-65% ethanol solution, and 70-80% ethanol solution to obtain S30, S45, S60, and S75 saponin components, respectively; 4) saponin hydrolysis: hydrolyzing the S45 saponin component to obtain hydrolyzed saponin; 5) saponin component recombination: recombining the saponin components obtained in steps 3) and 4) with the hydrolyzed saponin to obtain recombinant saponin from *Gleditsia sinensis*. This invention enables saponin purification and improves the surface activity of *Gleditsia sinensis*.

The invention relates to a template molecule, a molecularly imprinted polymer and application of the molecularly imprinted polymer in separation of naringin, and belongs to the technical field of plant component refining. According to the molecularly imprinted polymer disclosed by the invention, (S)-2-(4-hydroxymethyl phenyl)-7-(((2S, 3R, 4S, 5S, 6R)-3, 4, 5-trihydroxy-6-(hydroxymethyl) tetrahydro-2H-pyran-2-yl) oxy) benzodihydropyran-4-one is used as a template molecule, and the template molecule and a target product naringin can form multiple acting sites through non-covalent bonds such as hydrogen bonds and hydrophobic interaction, so that the target product naringin can be used for preparing the target product naringin. Therefore, specific binding is carried out, namely, high selectivity to naringin is achieved. The virtual template molecule is low in cost and can be used for large-scale preparation of molecularly imprinted polymers, and the extraction cost of naringin is remarkably reduced.

The invention provides a clay type lithium ore external field enhanced resource comprehensive utilization method, and relates to the field of hydrometallurgy. The method comprises the following steps: uniformly mixing clay type lithium ore with concentrated sulfuric acid, and carrying out low-temperature curing to obtain clinker; mixing the clinker with water, and then carrying out leaching reaction to obtain a lithium-rich aluminum leaching solution; carrying out evaporative crystallization on the lithium-rich aluminum leachate to obtain a mixed crystal of lithium sulfate and aluminum sulfate; performing high-temperature pyrolysis reaction on the mixed crystal to obtain an oxide; carrying out high-energy grinding and leaching on the oxide, and filtering to obtain a lithium-containing solution and crude aluminum oxide; and carrying out post-treatment on the lithium-containing solution to obtain the battery-grade lithium carbonate. According to the method, the technological process of the clay type lithium ore is optimized, synchronous leaching of valuable elements lithium and aluminum and efficient separation of the lithium element and the aluminum element are achieved, the problem that the cost is high when lithium is independently extracted is solved, cooperative utilization of clay type lithium ore resources is achieved, and the cost is reduced.

The invention relates to a method for recovering cobalt from a waste lithium battery leachate by using a beta-diketone eutectic solvent. The method uses the hydrophobic deep-eutectic solvent for extracting the organic phase. Wherein the deep eutectic solvent is prepared by mixing 4, 4, 4-trifluoro-1-(2-furyl)-1, 3-butanedione (HFTA) and trioctylphosphine oxide (TOPO) according to a molar ratio of 2: 1, and the catalyst is used for preparing the catalyst. The method comprises the following steps: taking a waste lithium ion battery leaching solution as an extraction water phase, wherein the extraction water phase contains CoSO4. 7H2O, NiSO4. 7H2O and Li2SO4; h2SO4 and NaOH are added into the extraction water phase to adjust the pH of the water phase, the extraction organic phase and the water phase are fully mixed according to the volume ratio of 1: 2-5: 2 and then stand still, phase separation is conducted, and an organic phase rich in cobalt and a water phase containing a small amount of cobalt are obtained. The method has the advantages of low energy consumption, simple process, high cobalt extraction efficiency and the like, the adopted extraction agent eutectic solvent is low in price, and the preparation method is simple and non-toxic.

The invention relates to a recombinant trichoderma reesei strain for producing brazitame protein as well as a construction method and application of the recombinant trichoderma reesei strain, and belongs to the technical field of biology. The recombinant trichoderma reesei strain is named as Trichoderma reesei DF230919, and is preserved in the China General Microbiological Culture Collection Center on August 9, 2024, and the preservation number of the recombinant trichoderma reesei strain is CGMCC (China General Microbiological Culture Collection Center) NO. 41488. According to the invention, a homologous recombination technology is utilized, QM6a [delta] pyr4 is taken as a host, a cellobiohydrolase gene cbh1 in trichoderma reesei is replaced with a Brazinein gene, and heterologous expression of Brazinein is realized in a recombinant trichoderma reesei strain; as the genome of the Trichoderma reesei is completely sequenced and widely researched, the construction and recombination of the Trichoderma reesei strain can be accurately controlled and regulated, the expression quantity and product purity of the Brazilian sweet protein can be effectively improved, and the Trichoderma reesei can grow under the condition of large-scale culture, so that the Trichoderma reesei strain can be widely applied to the field of culture of the Brazilian sweet protein. The Brazilian sweet protein is expressed in Trichoderma reesei through genetic engineering, so that the Brazilian sweet protein can be widely applied to industrial production of Brazilian sweet.

The invention relates to a broccoli pectin extraction method, which comprises: (1) raw material pretreatment: washing broccoli stems, slicing, carrying out hot air drying, and grinding into powder; (2) preparing a deep-eutectic solvent: selecting choline chloride as a reagent 1; and mixing the reagent 1 and the reagent 2 according to different molar ratios, incubating at the temperature of 80-100 DEG C, and magnetically stirring at the speed of 600-800 rpm until a clear and transparent liquid is formed. After the liquid is cooled to room temperature, the liquid is still not solidified, and the eutectic solvent is formed. (3) extraction treatment: mixing with a deep eutectic solvent (choline chloride and urea) according to a solid-liquid ratio of 1: (20-60), and continuously stirring at 60-80 DEG C at a rotating speed of 600-800 rpm for 1-3 hours to obtain an extracting solution; (4) centrifugal filtration: filtering the liquid with 300-mesh gauze, and then centrifuging for 15 minutes at the speed of 3500 rpm to obtain a supernatant; and (5) purifying and drying: adding absolute ethyl alcohol with the volume 3 times that of the supernate into the supernate, precipitating for 12 hours at room temperature, then centrifuging to obtain a pectin crude extract which is centrifuged for 15 minutes at 3500 rpm, and purifying and freeze-drying to obtain the broccoli stem pectin.

The utility model discloses a reusable gas extraction drilling hole packer, which comprises a gas pipe, a plurality of hole sealing units, a grouting pipe and a driving component, the gas pipe is used for gas extraction, the plurality of hole sealing units are arranged on the peripheral side of the gas pipe and are arranged at intervals along the extension direction of the gas pipe, and the grouting pipe is arranged on the periphery of the gas pipe. An annular cavity used for being filled with a hole sealing medium is limited between every two adjacent hole sealing units, each hole sealing unit comprises an elastic piece, the elastic pieces are used for expanding outwards when being compressed so as to seal the annular cavities, the grouting pipe communicates with at least one annular cavity, and the grouting pipe is used for conveying the hole sealing medium into the annular cavities. The driving assembly acts on the elastic pieces of the multiple hole sealing units and is used for applying acting force to the elastic pieces so as to compress the elastic pieces. The hole packer not only meets the use requirement of hole sealing in the gas extraction process, but also can be recycled after gas extraction is completed, so that waste of materials and funds is avoided, and the extraction cost is reduced.

The present invention provides a method for preparing a sea grape extract with antioxidant function, comprising the following steps: (1) washing and crushing the sea grapes; (2) adding an ethanol solution to the crushed sea grapes, heating, and obtaining a sea grape solution; (3) adding a composite enzyme to the sea grape solution in step (2) for enzymolysis; and then performing microwave extraction, with a microwave power of 260 to 320 W, to obtain a sea grape microwave liquid; the composite enzyme is prepared from pectinase, neutral protease, and cellulase; (4) filtering the sea grape microwave liquid in step (3), heating it, cooling it, and centrifuging it, collecting the supernatant, and obtaining a sea grape extract. The method for preparing the sea grape extract of the present invention not only fully extracts effective ingredients such as green algae polysaccharides, but also helps to reduce impurities, improve purity, and improve the activity of substances with antioxidant functions, is environmentally friendly and pollution-free, and reduces extraction costs.

The invention provides valerenic acid and an extraction method thereof, and belongs to the technical field of biochemistry. According to the method, valerianoic acid is extracted from valerian, valerianoic acid powder is obtained through first reflux extraction, impurity adsorption by activated carbon, dissolution by hot water and second reflux extraction in sequence, and the content of valerianoic acid reaches 6%-9%. According to the method for extracting the valerenic acid, provided by the invention, a chromatographic refining step in a traditional method is abandoned, expensive extraction equipment is not adopted, and low-boiling-point volatile organic solvents (such as petroleum ether, ethyl acetate and the like) are not used, so that the extraction cost of the valerenic acid is reduced, and the yield of the valerenic acid is also improved.

The invention discloses a preparation method and application of an acid-responsive aggregated carrot-source nano antioxidant, and belongs to the technical field of medical treatment. The carrot source nano antioxidant is prepared by five steps of cleaning, crushing, filtering, gradient centrifuging and extruding, can be applied to improvement of oxidative stress, promotion of good bone remodeling and effective treatment of osteoporosis, and has good practical application value.

This disclosure provides a method, apparatus, device, and storage medium for acquiring and applying an operations and maintenance (O&M) knowledge graph, which can be applied to the field of O&M service technology. The method includes: acquiring O&M resource data from a device information resource database; performing structured identification on the O&M resource data to obtain static O&M knowledge, which includes multiple first entities; acquiring real-time O&M service work order data; using a pre-trained knowledge extraction model to perform unstructured identification on the O&M service work order data to obtain dynamic O&M knowledge, which includes multiple second entities; acquiring historical relationships between the multiple first entities and the multiple second entities; inferring new relationships corresponding to the first entities in the O&M service work order data based on the first similarity between the multiple first entities, the second similarity between the multiple second entities, and the historical relationships; and obtaining an O&M knowledge graph based on the new relationships.

The present application belongs to the technical field of extraction and utilization of high-value components in industrial tail gas, and discloses a device and method for extracting hydrogen from industrial tail gas. The device comprises a heat exchanger, a high-temperature-resistant ceramic cylinder, a direct-current power supply and a cooler. Water vapor is used as a carrier gas to carry out the prepared hydrogen, which can reduce the electrolysis voltage and the power consumption for extracting hydrogen. The method of applying an external voltage is used to provide the driving force for hydrogen extraction, which can extract hydrogen from tail gas with low hydrogen concentration and improve the utilization rate of hydrogen resources. The device and method for extracting hydrogen from low-hydrogen-content high-temperature industrial tail gas are provided to solve the problem that hydrogen cannot be directly extracted from high-temperature industrial tail gas with low hydrogen concentration, resulting in a large amount of hydrogen resource waste. The device and method can directly extract hydrogen from high-temperature industrial tail gas with low hydrogen content by using high-temperature proton conductor ceramics, and water vapor is used as a carrier gas to carry the extracted hydrogen, which can reduce the power consumption for extracting hydrogen and efficiently prepare high-purity hydrogen.

This invention relates to the field of natural saponin separation and recombination, and particularly to a complex of saponin from *Gleditsia sinensis* and saponin from *Camellia oleifera* and its preparation method. The method includes: 1) extracting saponin from *Gleditsia sinensis* pericarp and *Camellia oleifera* cake, respectively, to obtain crude saponin solutions from *Gleditsia sinensis* and crude saponin from *Camellia oleifera*; 2) using macroporous adsorption resin AB-8 as a resin separation column to elute and remove impurities from the crude saponin solutions from *Gleditsia sinensis* and crude saponin from *Camellia oleifera*; 3) eluting the obtained saponin solutions from *Gleditsia sinensis* and *Camellia oleifera* with 25-35% ethanol solution, 40-50% ethanol solution, 55-65% ethanol solution, and 70-85% ethanol solution, respectively, to obtain saponin components from *Gleditsia sinensis* and saponin components from *Camellia oleifera*; 4) recombining the saponin components from *Gleditsia sinensis* and *Camellia oleifera* to obtain the complex of saponin from *Gleditsia sinensis* and *Camellia oleifera*. This invention can improve the purity and surface activity of natural saponins, overcoming the disadvantage of the high critical micelle concentration of *Camellia oleifera*.

The invention discloses a rectifying still residue extraction method, and belongs to the field of environmental protection and the technical field of hazardous waste harmless resource utilization. The technical problem that the recovery rate of residual NMP in the current rectifying still residues is not high is solved. The method comprises the following steps: soaking the residual lignosulfonate in an NMP rectifying still, adding an organic solvent for treatment, and then rectifying to obtain NMP. According to the method, the NMP is extracted by utilizing the boiling point difference between the organic solvent and the NMP rectifying still residue and temperature-controlled rectification, and the lignosulfonate is used as a water reducing agent to be compounded with water, so that the moisture required when the solid still residue is dissolved is reduced, and the flowability of the still residue after the organic solvent is added is improved. In addition, the organic solvent is matched with water and NMP (N-Methyl Pyrrolidone) in rectifying still residues, so that the azeotropic phenomenon is reduced. According to the method, the NMP rectifying still residues which cannot be recycled are pretreated with chemical reagents such as diethylene glycol and then rectified, so that a large amount of NMP is recovered, and the utilization efficiency of NMP is greatly improved.

The invention is applicable to the technical field of chlorogenic acid purification, and provides a method for cold extraction of chlorogenic acid in a honeysuckle raw material, which comprises the following steps: putting the honeysuckle raw material into an extraction tank, compacting the top, injecting a prepared sodium hydroxide solution into the extraction tank to completely immerse the material, and extracting for 2-4 hours at the top, after soaking for 24-48 hours, percolating according to the flow rate of 0.2-1L / min, and controlling the concentration of the sodium hydroxide solution to be 0.02%-0.15%; then the extraction tank is controlled to discharge liquid medicine into the storage tank at the same flow speed, the liquid medicine in the storage tank is pumped into the concentrator to be concentrated until the required density is 1.02-1.10, a concentrated solution is obtained, the concentrated solution is dried to obtain powder, the extraction temperature is low, components are not damaged, the extraction efficiency is high, the extraction cost is low, and the safety is good.

The invention discloses an extraction method of paulownia flower polyphenol capable of inhibiting alpha-glucosidase activity, and relates to the technical field of paulownia flower polyphenol extraction, paulownia flowers are crushed and sieved, then a urea solution of choline chloride is added into powder according to the material-liquid ratio of 0.1 g: 10 ml, the water content is 30%, the ultrasonic time is 5-45 min, the ultrasonic power is 200-400 W, the ultrasonic temperature is 20-60 DEG C, centrifugation and suction filtration are performed, and the paulownia flower polyphenol is obtained. And carrying out reduced pressure distillation, drying, column chromatography separation, eluent collection, concentration and drying to obtain the paulownia flower polyphenol extract. According to the polyphenol extraction method disclosed by the invention, the ultrasonic-assisted deep eutectic solvent is used for performing deep eutectic solvent extraction on the paulownia flower, the extraction filtration is combined for impurity removal, the macroporous resin adsorption purification is performed, and a series of technical means are adopted, so that the prepared polyphenol is relatively high in purity and yield, and the alpha-glucosidase activity inhibition capability is strong.

The invention relates to a volcanic rock type uranium ore beneficiation and tailing discarding process, and belongs to the technical field of uranium mining and metallurgy. The technology comprises the steps that after raw ore is deslimed, a first batch of heavy liquid with the density gradient being 0.01-0.07 g / cm < 3 > and different density gradients is prepared, the first batch of heavy liquid is subjected to first-time heavy liquid separation, ore continues to be screened, then second-time heavy liquid separation is conducted, and the uranium grade and the yield of ore products with different particle size grades and different density grades are analyzed; the product yield B% of the ore with the uranium grade larger than or equal to 0.022 in each particle size level is determined; and dense medium cyclone separation is conducted, parameters of dense medium cyclone separation are controlled, so that the concentrate yield of dense medium separation of the ores of all particle size levels is (B + / -20)%, and finally low-intensity magnetic separation is conducted, and dense media are recycled. According to the method provided by the invention, a large amount of tailings can be discarded under the condition of coarse particles, the process handling capacity of subsequent uranium hydrometallurgy extraction is reduced, and the production cost is reduced.

The invention provides a helium-3 and helium-4 co-production system, and relates to the field of isotope separation. The helium-3 and helium-4 co-production system comprises a helium-4 liquefying device, a helium-3 enriching device and a helium-3 purifying device; the helium-4 liquefying device is used for liquefying helium to form liquid helium; the helium-3 enriching device is communicated with the helium-4 liquefying device, and the helium-3 enriching device is used for increasing the concentration of helium-3 in liquid helium; the helium-3 purification device is communicated with the helium-3 enrichment device and the helium-4 liquefaction device, and the helium-3 purification device is used for purifying helium-3. According to the helium-3 and helium-4 co-production system provided by the invention, helium-3 is continuously purified on the basis of helium extraction, and helium-3 can be extracted on a large scale at a relatively low cost. The main economic cost of the process is at the front end, namely the helium extraction part and the helium liquefaction part in the helium-rich natural gas, the cost of a helium-3 enrichment and helium-3 purification device is low, and the helium-3 extraction cost is greatly reduced on the whole.