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Technique for extracting and refining DHA enriched fatty acid from Crypthecodinium cohnii

A technology for cryptodinia and fatty acid, applied in the biological field, can solve the problems of simple content, no description of the fatty acid extraction method in microalgae, etc., and achieves the effects of simple operation, reduced extraction cost, and improved quality

Active Publication Date: 2008-04-30
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, domestic patents mainly focus on the separation and extraction of long-chain polyunsaturated fatty acids from fish oil. There are only two patents related to the method of microalgae fermentation to produce long-chain polyunsaturated fatty acids (application numbers: 94106621.5 and 00135338.1), but the content is relatively simple , without a detailed description of the fatty acid extraction method from microalgae

Method used

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  • Technique for extracting and refining DHA enriched fatty acid from Crypthecodinium cohnii
  • Technique for extracting and refining DHA enriched fatty acid from Crypthecodinium cohnii
  • Technique for extracting and refining DHA enriched fatty acid from Crypthecodinium cohnii

Examples

Experimental program
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Effect test

Embodiment 1

[0052] Example 1 Fatty acid extraction and refining of algae harvested by fermentation in a 5L fermenter

[0053] Carry out 5 L tank fermentation according to above-mentioned cultivation method, harvest algae body. Pour the fermented liquid into the solvent tank, add 45g ferric chloride to make the cells flocculate and precipitate, after standing still, release the upper layer solution, add 800ml alcohol to the tank to make more bacteria settle, and release the upper layer liquid, The bacterial cells obtained above are centrifuged with a centrifuge to realize further solid-liquid separation. Use NaOH to prepare an aqueous solution with a pH of 14, pour the bacteria obtained in the previous step into the alkali wall, then send the cells into a colloid mill, grind the material into small particles, and send it to a homogenizer for high-pressure crushing to obtain Broken bacteria. Centrifuge again (speed 8000rpm) to remove water, pour the crushed thalline into the extraction ta...

Embodiment 2

[0056] Example 2 Fatty acid extraction and refining of algae harvested by fermentation in a 5L fermenter

[0057] Carry out 5 L tank fermentation according to above-mentioned cultivation method, harvest algae body. Pour the fermentation broth into a solvent tank, add 45g of aluminum sulfate to make the cells flocculate and precipitate, and after standing still, release the upper layer solution; add 800ml alcohol to the tank to make more bacteria settle, and release the upper layer liquid; Centrifuge the bacteria obtained above to realize further solid-liquid separation. Use NaOH to prepare an aqueous solution with a pH of 13, pour the bacteria obtained in the previous step into the alkali wall, then send the cells into a colloid mill, grind the material into small particles, and send it to a homogenizer for high-pressure crushing to obtain Broken bacteria. Centrifuge again (speed 8000rpm) to remove water, pour the broken bacteria into the extraction tank, pour it into the ex...

Embodiment 3

[0060] Example 3 Fatty acid extraction and refining of algae harvested by fermentation in a 5-ton fermenter

[0061] Carry out 5 ton tank fermentation according to above-mentioned culture method, harvest algae body. Pour the fermentation broth into a solvent tank, add 50kg of polyaluminum chloride to flocculate and precipitate the cells, and release the upper layer solution after standing still; add 15L alcohol to the tank to make more bacteria settle, and release the upper layer liquid as well; The bacterial cells obtained above are centrifuged with a centrifuge to realize further solid-liquid separation. Use NaOH to prepare an aqueous solution with a pH of 12, pour the bacteria obtained in the previous step into the alkali wall, then send the cells into a colloid mill, grind the material into small particles, and send it to a homogenizer for high-pressure crushing to obtain Broken bacteria. Centrifuge again (speed 8000rpm) to remove water, pour the broken bacteria into the...

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Abstract

The invention discloses a process for extracting and refining fatty acid rich in DHA from Cryptidinosa. The process first flocculates the fermentation broth of Crypthecodinium cohnii and then separates the solid from the liquid. After breaking the wall with alkali, the cells are mechanically broken, and then the broken bacteria are extracted with an organic solvent to obtain DHA crude oil; the DHA crude oil is passed through DHA essential oil is obtained after degumming, alkali refining, decolorization and deodorization. The method is simple to operate and can reduce the cost of extracting DHA oil. Only organic solvents are used in the operation process, and there is no residue in the end. After refining, the quality of DHA oil is improved. The DHA content in the extract is about 40-50%, which is suitable for DHA Large-scale production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a process for extracting and refining fatty acid rich in DHA from Crypthecodinium cohnii. Background technique [0002] DHA (Docosahexaenoic acid, 22:6 △4.7.10.13.16.19 , full name docosahexaenoic acid) is an important long-chain polyunsaturated fatty acid (polyunsaturated fatty acid, referred to as PUFA), belonging to the n-3 series. As an essential component of cell membrane synthesis, DHA has the functions of enhancing eyesight and promoting the development of brain cells. It is an essential component for the growth and development of infant brains and the maintenance of normal brain functions for adults. It is known as "brain gold". DHA can also lower blood fat, prevent and treat arteriosclerosis, anti-inflammatory, anti-cancer and other effects. Therefore, DHA has received extensive attention from the food and medical circles. [0003] Traditional DHA production raw materials m...

Claims

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Application Information

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IPC IPC(8): C07C57/03C07C51/42
Inventor 黄和任路静李霜胡南金明杰
Owner NANJING UNIV OF TECH
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