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Method simultaneously extracting lipid and protein from corn plantule

A technology for extracting oil and protein, which is applied in the field of oil and protein extraction, can solve the problems of reducing protein functional and nutritional properties, limiting protein deep processing and reuse, protein denaturation, etc., achieving environmental pollution, mild extraction conditions, and overcoming The effect of protein denaturation

Inactive Publication Date: 2009-04-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the leached meal has to undergo high-temperature desolventization, the protein in the meal will be denatured, and the functional and nutritional properties of the protein will be reduced. Therefore, the grain germ protein can only be used as feed or even as a fertilizer, resulting in a great waste of resources. Deep processing and reuse of protein

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1, a kind of method for simultaneously extracting oil and protein from grain germ, the steps are:

[0018] (1) corn germ powder is pure, sieved 100 orders, makes corn germ powder;

[0019] (2) Preparation of the reverse micellar solution: mix the surfactant SDS and the organic solvent n-hexane according to the ratio of 2:50 (w / v), stir to dissolve it, and after it is completely dissolved, add 0.08 times the volume of the organic solvent , pH7.0, 0.2mol / L KCl phosphate buffer solution, then centrifuged at 3000r / min for 20min, the obtained transparent and clear system is the reverse micellar solution;

[0020] (3) Pre-extraction: Mix corn germ powder and reverse micellar solution at a ratio of 2:100 (w / v), stir (1000r / min, 75min), then centrifuge at 3000r / min for 20min, and the supernatant is the pre-extraction Extract;

[0021] (4) Post-extraction: Mix the pre-extraction solution with 1.25 times volume, pH6, 1.5mol / L KCl phosphate buffer solution, and ultras...

Embodiment 2

[0024] Embodiment 2, a kind of method extracting oil and protein simultaneously from grain germ, comprises the following steps successively:

[0025] (1) wheat germ powder is pure, sieved 200 orders, makes wheat germ powder;

[0026] (2) Preparation of reverse micellar solution: mix the surfactant AOT with the organic solvent isooctane in a ratio of 3:50 (w / v), stir to dissolve it, and add 0.06 times the organic solvent after it is completely dissolved Volume, pH8.0, 0.1mol / L KCl phosphate buffer solution, then centrifuged at 3000r / min for 20min, the obtained transparent and clear system is the reverse micellar solution;

[0027] (3) Pre-extraction: Mix wheat germ powder and reverse micellar solution in a ratio of 1:100 (w / v), and use ultrasonic-assisted extraction (ultrasonic parameters are as follows: ultrasonic power, 350W, ultrasonic time, 20min, intermittent mode, Pulse 2s, intermittent 2s), then centrifuge at 3000r / min for 20min, the supernatant is the pre-extraction; ...

Embodiment 3

[0031] Embodiment 3, a kind of method for simultaneously extracting oil and protein from grain germ, comprises the following steps successively:

[0032] (1) Rice germ powder is pure, sieved 150 orders, makes rice germ powder;

[0033] (2) Preparation of reverse micellar solution: mix the surfactant CTAB and the organic solvent isooctane in a ratio of 4:50 (w / v), stir to dissolve it, and add 0.04 times the organic solvent after it is completely dissolved Volume, pH9.0, 0.3mol / L KCl phosphate buffer solution, then centrifuged at 3000r / min for 20min, the obtained transparent and clear system is the reverse micellar solution;

[0034] (3) Pre-extraction: Mix rice germ powder and reverse micellar solution in a ratio of 3:100 (w / v), and use ultrasonic-assisted extraction (ultrasonic parameters are as follows: ultrasonic power 450W, ultrasonic time 30min, intermittent mode: pulse 3s , intermittent 3s), then centrifuged at 3000r / min for 20min, the supernatant was the pre-extraction;...

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Abstract

The invention discloses a method for extracting grease and protein simultaneously from cereal germs, and pertains to the technical field of comprehensive utilization of agricultural byproducts. The method comprises the comprehensive utilization of technologies of reversed micellar extraction, ultrasonic-associated extraction, protein refining, grease refining, drying and so on. Cereal germs are sieved after crushing, added into reversed micellar solution, stirred or ultrasonic forward extracted and centrifugated to obtain forward extraction solution, and the forward extraction solution is processed by ultrasonic backward extraction and centrifugation, the upper layer is extracted by ethanol to remove a residual surfactant, a solvent is removed to obtain grease and after water, salt and the surfactant are removed from the lower layer, cereal germ protein product is obtained; or the solvent in the forward extraction solution is directly removed, protein in a residual solid substance is precipitated by a mixed solvent, and then washing and drying are carried out to obtain the cereal germ protein product. The method has simple technology and low cost, solves the problem that the traditional grease production causes damage to protein, improves reutilization value of cereal germ protein, and is an environmental friendly production method. The product has the advantages of high yield rate, high purity, low chemical modification and the like.

Description

technical field [0001] The invention discloses a method for simultaneously extracting oil and protein from grain germs, relates to an oil and protein extraction method, and belongs to the technical field of comprehensive utilization of agricultural and sideline products. Background technique [0002] Grain germ is generally a by-product obtained in the process of grain processing. Although the quality of grain germ accounts for only a dozen percent of the grain, it concentrates most of the nutrition of the grain grain, most of the fat and minerals of the grain grain, and a considerable part of it. Most of the protein in grains exists in the germ, and the grain germ is also rich in carbohydrates, amino acids, vitamins and minerals, and is known as "the source of nutrition given by God" abroad. As a vegetable protein and fat resource, grain germ is second only to soybean in protein and fat content, and its protein and fat quality and nutritional value are comparable to soybean...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/18C07K1/14C11B3/00
Inventor 朱科学孙晓宏李飞周惠明彭伟
Owner JIANGNAN UNIV
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