The invention relates to a method of silane enwrapping II-VI semiconductor quantum dots. The invention comprises the preparation of the II-VI semiconductor quantum dots, inorganic enwrapping and silane enwrapping. The silane enwrapping of the II-VI semiconductor quantum dots comprises the steps that: dissolving polyoxyethylene nonyl phenyl ether into cyclohexane to form stable reversed micellar solution, then, adding trichloromethane solution of the II-VI semiconductor quantum dots which are inorganically enwrapped into the reversed phase micellar solution of the step a, then, adding gamma-mercaptopropyltrimethoxysilane, regulating the pH value range to 8.0-10.0, adding ethyl orthosilicate, then, functionalizing, and lastly, obtaining the II-VI semiconductor quantum dots which are wrapped by the silane and functionalized. The invention increases the stability of the quantum dots in buffering liquid through controlling the thickness of the quantum dots silane layer, the fluorescence can be kept well, the photobleaching and the optical quenching effect of the quantum dots can be reduced, the surface functionalization decoration of the quantum dots can be realized through selecting different functionalized radical, the biological application basis is provided after the quantum dots are dissolved in water, and the quantum dots which are treated by the saline can be the first choice in the application of thebiological fluorescence labeling field.