The invention discloses an ejecting-type jet flow cavitation generator and a cavitation cell disruption device. The cavitation generator comprises a nozzle, a suction chamber, a throat pipe, a diffuser and an outlet pipe. The nozzle is connected in the suction chamber in a sleeved manner, and a suction pipe is arranged on the side of the suction chamber. The cavitation cell disruption device comprises a container, a high-pressure centrifugal pump and the above cavitation generator, wherein the container is used for storing cells to be disrupted, the high-pressure centrifugal pump is connected with the container through a water pipe, and the cavitation generator is connected with the high-pressure centrifugal pump through the pipe. Valves are respectively arranged on an entrance of the nozzle, the suction pipe and the outlet pipe to control degree of cavitation. In the cavitation cell disruption device, high-pressure liquid sucks up low-pressure liquid to cause cavitation to happen in the tail end of the suction chamber and the throat pipe, cells are disrupted by microjet and shock waves produced from bubble collapse, and drastic shearing actions between the other two fluids can also have tearing effect to the cells. The ejecting-type jet flow cavitation generator and the cell disruption device for cavitation cells are simple in structure, low in cost, free of moving components, reliable in operating, strong in cavitation transport, high in crushing efficiency, and thereby applicable to mass application.