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Preparation of magnetic silicon dioxide microsphere with metallic ion chelated surface and use thereof

The invention relates to a method for preparing magnetic silicon dioxide microspheres with the surfaces chelated with metal ions; the method of the inventioncomprises the following steps of preparation of the magnetic silicon dioxide microspheres, epoxidation of the magnetic silicon dioxide microspheres, carboxylation of the magnetic silicon dioxide microspheres and chelation technique of metal ions. Compared with the existing preparing method, the method of the invention has the advantages of reasonable design, simple operation and large amount of chelation of metal, etc. The magnetic silicon dioxide microspheres with the surfaces chelated with the metal ions, which are prepared by adopting the method, contain cobalt ions, have stronger bonding force with the modified microspheres so as to lead the cobalt ions to be not easy to leak out, and have higher selectivity and proper bonding strength with histidine on the protein, thus leading the magnetic silicon dioxide microspheres with the surfaces chelated with the metal ions to have high selectivity to hexameric histidine tag protein, and being easy to realize the separation and the purification of the target protein; the magnetic silicon dioxide microspheres with the surfaces chelated with the metal ions, which are prepared by adopting the method, can be used for separating the hexameric histidine tag protein.
Owner:SHAANXI NORMAL UNIV

Temperature-resistant water-soluble copolymer, preparation and use thereof

InactiveCN101274974AImprove solution performanceImprove rigidityDrilling compositionSolubilityPersulfate
The invention discloses a copolymer with temperature resistance and water solubility and the preparation method and application thereof, which is characterized in that: 20 portions of acrylic amide, 0.1 to 10 portions of anion monomer or/and cation monomer, 0.05 to 3 portions of fluorine-containing styrene or/and perfluoroalkyl styrene hydrophobic monomer, 0.5 to 60 portions of surface active agent and 50 to 700 portions of deionized water are put into a three-necked reaction bulb; after the solution pH is regulated to be equal to 2.5 to 9 and N2 is connected for 30 minutes, 0.001 to 0.2 portion of an evocating agent of persulfate is added under temperature of 20 to 70 DEG C, reacting for 6 to 36 hours, and PATF is prepared. Then water is used for diluting so as to prepare PATF concentrated solution, thus obtaining a copolymer that has medium molecular weight and water solubility, is high-temperature resistant, has an ability of molecular association and can be used in high temperature and high salinity reservoir. The copolymer is prepared into aqueous solution with a mass concentration of 0.3 to 3g/L and a surface-active-agent concentration of 0.01 to 4mmol/L, which is put in a blending container with a dasher and is stirred even at room temperature to prepare a polymer oil-displacing agent which is high-temperature resistant, high tackifying and anti-sharing and has excellent aging resistance under high salinity and the temperature of 110 DEG C. The prepared oil-displacing polymer has excellent solution properties owing to the supermolecular structure formed by the association between molecules of fluorine-containing cinnamene or/and alkylbenzene hydrophobic groups, thus having favorable application prospects in high temperature and high salinity reservoir exploitation.
Owner:CHENGDU UNIVERSITY OF TECHNOLOGY

Novel method for modifying graphene with polymer

The invention discloses a novel method for modifying graphene with polymer. By introducing polydopamine and combining covalent modification with non-covalent modification, the method increases the stability of the modified product structure under the premise of ensuring the integrity of the graphene structure. Firstly, by utilizing the strong adhesion of dopamine, the dopamine is self-polymerized to form a film on the surface of a graphene sheet, moreover, 2-Bromo-2-methylpropionyl bromide (BiBB) as common initiator for atom transfer radical polymerization (ATRP) is grafted on the dopamine by amidation, at the same time, graphene oxide (GO) is partially reduced by the dopamine (DA), and thereby graphene macroinitiator RGO@PDA-Br is obtained. Polymeric monomer is then added, so that the ATRP of the polymeric monomer is initiated on the surface of the graphene, and thereby a polymer-modified graphene material is obtained. By controlling conditions such as monomer concentration and reaction time, the novel method can obtain modified graphene materials with different densities and chain lengths of polymer grafted on the surfaces, meeting the characteristics of controllable/living ATRP. In addition, the feasibility and universality of the novel method for modifying graphene with polymer put forward by the invention are applicable to various monomers suitable for ATRP.
Owner:JIANGNAN UNIV

Protein molecular imprinting polyion liquid membrane electrochemical transducer

The invention relates to the technical field of electro-analytical chemistry and protein identification transducers, and discloses a synthesizing method of functionalized ionic liquid and a preparation method and application of a molecular imprinting electrochemical transducer composed of the functionalized ionic liquid, carboxylation multiwalled carbon nanotubes and glassy carbon electrodes. The preparation method of the molecular imprinting electrochemical transducer comprises the steps that the polymerizable amino-functionalized ionic liquid is used as a functional monomer, BSA is used as template protein, N, N'-methylene bisacrylamide is used as a cross-linking agent, an oxidation-reduction system composed of ammonium persulfate and TEMED is used as an initiator, after polymerization, a molecularly imprinted polymer film is formed on the surfaces of the glassy carbon electrodes decorating the carboxylation multiwalled carbon nanotubes, and then the template protein is eluted to obtain the molecular imprinting electrochemical transducer which can identify template protein in a specific mode. The molecular imprinting electrochemical transducer has the advantages of being simple in preparation, low in material cost, high in selectivity, good in biocompatibility, and capable of being used for identifying and detecting protein in aqueous solution.
Owner:SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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