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Protein molecular imprinting polyion liquid membrane electrochemical transducer

An ionic liquid and protein technology, used in the synthesis of functionalized ionic liquids and the preparation of protein molecularly imprinted electrochemical sensors, can solve the problems of poor selective recognition of proteins, no biocompatibility, complicated and time-consuming operations, etc. , to achieve good water solubility and biocompatibility, good stability and repeatability, and good selectivity.

Inactive Publication Date: 2014-11-12
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the functional monomers or imprinting materials used in the current methods of protein molecular imprinting are not biocompatible, and there are also shortcomings such as poor ability to selectively recognize proteins, complex and time-consuming operations, and cannot be reused.

Method used

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  • Protein molecular imprinting polyion liquid membrane electrochemical transducer
  • Protein molecular imprinting polyion liquid membrane electrochemical transducer
  • Protein molecular imprinting polyion liquid membrane electrochemical transducer

Examples

Experimental program
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Effect test

Embodiment 1

[0044] A carboxylated multi-walled carbon nanotube, the preparation method of which is as follows:

[0045] 50mg multi-walled carbon nanotubes (MWCNT) dissolved in 6mL4mol L -1 Nitric acid, heated to reflux for 24h. After the reaction was completed, the acidified product was washed by centrifugation with acetonitrile to pH 7, then washed with ethanol, and dried at 25°C. Its FT-IR spectrum as figure 1 As shown in (A), the SEM pattern is as figure 1 (B) shown.

Embodiment 2

[0047] A glassy carbon electrode modified by carboxylated multi-walled carbon nanotubes, the preparation method of which is as follows:

[0048] Polish the glassy carbon electrode with a diameter of 3mm to a bright mirror surface on a polishing cloth with alumina powder with a particle size of 0.05 μm, rinse it with secondary water, and then use nitric acid solution (65wt% nitric acid mixed with secondary water in equal volumes) solution), absolute ethanol and secondary water for 2 min, and finally with N 2 Blow dry and set aside. Weigh a certain amount of carboxylated multi-walled carbon nanotubes (MWCNT-COOH) prepared in Example 1, add them into secondary water, and disperse them uniformly by ultrasonic, so that the concentration of MWCNT-COOH is 0.5 mg·mL -1 . Use a pipette to draw 6.0 μL MWCNT-COOH solution and drop-coat it on the surface of the glassy carbon electrode, and dry it at room temperature to obtain a carboxylated multi-walled carbon nanotube-modified glassy c...

Embodiment 3

[0050] 1-vinyl-3-aminopropylimidazolium tetrafluoroborate ionic liquid, its synthesis method is as follows:

[0051] Dissolve 4.4g of 3-bromopropylamine hydrobromide (CAS No.: 5003-71-4) in 30mL of anhydrous acetonitrile, N 2 protection, raised the temperature to 55°C, then added 30mL of anhydrous acetonitrile solution containing 2.8g N-vinylimidazole dropwise in 2h, then continued to react for 12h, removed the solvent, added water and toluene twice for washing, and separated the liquid The aqueous layer was dissolved in methanol and Na was added dropwise 2 CO 3 Saturated methanol solution to pH 9. Evaporate the solvent, add a saturated solution of sodium tetrafluoroborate to the product, stir for 1 h, remove water by evaporation, wash with methanol centrifugally, and evaporate methanol to obtain light yellow 1-vinyl-3-aminopropylimidazolium tetrafluoroborate ionic liquid.

[0052] Product characterization results: 1H NMR (D 2 O)δ: 9.07(s, 1H), 7.75(s, 1H), 7.58(s, 1H), 7....

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Abstract

The invention relates to the technical field of electro-analytical chemistry and protein identification transducers, and discloses a synthesizing method of functionalized ionic liquid and a preparation method and application of a molecular imprinting electrochemical transducer composed of the functionalized ionic liquid, carboxylation multiwalled carbon nanotubes and glassy carbon electrodes. The preparation method of the molecular imprinting electrochemical transducer comprises the steps that the polymerizable amino-functionalized ionic liquid is used as a functional monomer, BSA is used as template protein, N, N'-methylene bisacrylamide is used as a cross-linking agent, an oxidation-reduction system composed of ammonium persulfate and TEMED is used as an initiator, after polymerization, a molecularly imprinted polymer film is formed on the surfaces of the glassy carbon electrodes decorating the carboxylation multiwalled carbon nanotubes, and then the template protein is eluted to obtain the molecular imprinting electrochemical transducer which can identify template protein in a specific mode. The molecular imprinting electrochemical transducer has the advantages of being simple in preparation, low in material cost, high in selectivity, good in biocompatibility, and capable of being used for identifying and detecting protein in aqueous solution.

Description

technical field [0001] The invention relates to the technical fields of polyionic liquid, western blot, molecular recognition and electrochemical sensor. It specifically relates to a synthesis method of a functionalized ionic liquid, and a preparation method and application of a protein molecularly imprinted electrochemical sensor composed of the ionic liquid / carboxylated multi-walled carbon nanotube / glassy carbon electrode. Background technique [0002] At present, technologies such as quantitative analysis and biosensing that are selective for proteins are almost all based on antigen-antibody immune reactions; antibody systems are not only expensive, but also have harsh conditions for use, and when recognizing proteins, their structures are unstable, so they can only be used once. Molecular imprinting technology is a new separation technology that is artificially synthesized and has the function of molecular recognition. The synthesis of molecularly imprinted polymers th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/333C08F222/38C08J9/28
Inventor 李春涯韩苗王炎英
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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