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This invention provides a method for detecting trans-4,5-epoxy-2(E)-decaldehyde based on LC-MS / MS. First, trans-4,5-epoxy-2(E)-decaldehyde standards are prepared into standard solutions of different concentrations. Then, the standard solutions are derivatized by solid-phase extraction with 2,4-dinitrophenylhydrazine (DNPH) to obtain analytical samples containing standard solutions of different concentrations. Next, LC-MS / MS analysis is performed on the analytical samples of different concentrations of standard solutions to obtain a standard curve with standard concentration as the abscissa and peak area as the ordinate. The sample to be tested is detected using LC-MS / MS, and the content of trans-4,5-epoxy-2(E)-decaldehyde in the sample is determined using the standard curve. This invention achieves accurate qualitative and quantitative analysis of this compound and establishes a standardized platform for the role of this compound in systemic health and disease.