This invention discloses an Angiopep-2 modified fluorescent
polyamide-amine dendritic
polymer and its preparation method, as well as a method for preparing nanoparticles based thereon, belonging to the field of
nanomaterials technology. The preparation method includes the following steps: mixing the
polyamide-amine dendritic
polymer with a first
phosphate buffer to obtain solution I; mixing
maleimide-
polyethylene glycol-N-hydroxysuccinimide with the first
phosphate buffer to obtain solution II; mixing solution I and solution II and reacting them, followed by
ultrafiltration purification to obtain intermediate A; dissolving Angiopep-2 in a second
phosphate buffer and reacting it with intermediate A, followed by freeze-
drying to obtain intermediate B; dissolving intermediate B in a
dimethyl sulfoxide solution to obtain solution III; dissolving
fluorescein isothiocyanate in a
dimethyl sulfoxide solution to obtain
solution IV; mixing solutions III and IV and reacting them under a protective gas
atmosphere in the dark; freeze-
drying to obtain the Angiopep-2 modified fluorescent
polyamide-amine dendritic
polymer.