The invention provides a working electrolyte for a flame-retardant wide-temperature high-voltage aluminum electrolytic capacitor, which comprises the following components in percentage by weight: 45-80% of main solvent, 0.1-5% of oxidation film stabilizer, 5-15% of secondary solvent, 0.2-3% of hydrogen absorption agent, 5-10% of solute, 5-15% of flame retardant and 5-15% of spark voltage increasing agent. The invention also provides a preparation method of the electrolyte and application of the electrolyte in preparation of a high-voltage electrolytic capacitor. In the electrolyte, appropriate solvent combination is adopted, thus the wide temperature from -40 DEG C to 105 DEG C is realized; a composite solute of an organic carboxylate with a branched chain and a straight-chain carboxylate with a long carbon chain is adopted, thus the stability is ensured, and the cost is controlled; the appropriate spark voltage increasing agent, oxidation film stabilizer and hydrogen absorption agent are added, thus the spark voltage of the electrolyte is increased, and the characteristic parameters of the aluminum electrolytic capacitor are not deteriorated after the aluminum electrolytic capacitor is used for a long time; and the flame retardant is added, thus the aluminum electrolytic capacitor is difficult to burn after striking fire.