10 results about "Covalent modification" patented technology

The present disclosure provides a system for treating melioidosis, comprising a first antimicrobial agent comprising trimethoprim at a subinhibitory concentration and a second antimicrobial agent comprising a FolE2 enzyme inhibitor selected from dehydrocostus lactone, parthenolide, or β-lapachone, wherein the combination of the first antimicrobial agent and the second antimicrobial agent exhibits chemical synthetic lethality against Burkholderia pseudomallei while having minimal effect on commensal bacteria. The subinhibitory concentration of trimethoprim ranges between 5 μM and 30 μM. The FolE2 enzyme inhibitor acts as a mechanism-based inhibitor that covalently modifies a catalytic cysteine residue (Cys154) of the FolE2 enzyme. The combination exhibits greater than 90% growth suppression against Burkholderia pseudomallei. Methods for treating melioidosis and identifying antimicrobial drug targets using chemical synthetic lethality screening approaches are also provided.

A porous ePTFE article coated with a hydrophilic coating is provided. The coating may be modified by covalent modification of the PVOH coating. The covalent modification may include grafting small molecules, polymers to or from a PVOH backbone, modifying PVAc precursors of PVOH, cross-linking polymers via thermal or photochemistry, cross-linking polymers with a multifunctional cross-linking agent, and the like, and combinations thereof.

This invention belongs to the fields of food biotechnology and food processing, and discloses a method, product, and application for regulating the allergenicity of soybean protein based on heat-induced proanthocyanidin covalent modification. This invention aims to solve the problem of unstable regulation of soybean protein allergenicity during heat processing, and provides a stable and differentiated method for reducing allergenicity. The core of the method lies in: under heat treatment conditions, proanthocyanidins simultaneously covalently modify soybean 7S and 11S globulins, achieving differentiated regulation of their allergenicity. Products using this method exhibit the following specific pattern of allergenicity: 7S globulin products are significantly lower than heat-treated products; 11S globulin products are lower than heat-treated products and natural proteins. This low-allergenicity pattern remains stable after in vitro simulation of adult gastrointestinal digestion. This invention provides a targeted, mild, and efficient allergen regulation strategy, and the resulting products can be used to develop low-allergenic foods, special medical purpose formula foods, and dietary supplements.

This application discloses an ion exchange membrane, its preparation method, application, and an organic electrosynthesis apparatus. The ion exchange membrane includes a base membrane and a functional layer. The functional layer is loaded onto the base membrane, and the material of the functional layer includes two-dimensional nanomaterials covalently modified with organic molecular fragments. The organic molecular fragments are used to regulate the interlayer spacing of the two-dimensional nanomaterials. The interlayer spacing of the two-dimensional nanomaterials covalently modified with organic molecular fragments in the functional layer is 0.8 nm to 1.5 nm. This ion exchange membrane includes a base membrane and a functional layer, and the material of the functional layer includes two-dimensional nanomaterials covalently modified with organic molecular fragments. By covalently modifying the two-dimensional nanomaterials with organic molecular fragments, strong interaction forces are introduced between the nanosheets of the two-dimensional nanomaterials, effectively "anchoring" the interlayer spacing of the nanosheets. When the ion exchange membrane is immersed in a solvent, the risk of a large amount of solvent entering the interlayer of the nanosheets and causing severe swelling of the ion exchange membrane can be reduced.

This invention belongs to the field of comprehensive utilization of mineral resources and inorganic non-metallic materials technology, specifically relating to a method for preparing hydrophobic mesoporous silica using gold tailings. The method uses gold tailings as raw material. First, the gold tailings are finely ground, activated, and calcined at high temperature to control the output particle size. Then, based on the specific gravity difference, iron-containing impurities are separated by air classification to obtain silica-rich powder. Next, the silica-rich powder is converted into a sodium silicate solution using alkali fusion, and further, silica slurry is precipitated from the sodium silicate solution by acid precipitation. Finally, a hydrophobic modifier is added to the silica slurry for in-situ surface covalent modification treatment. After washing and drying, a stable hydrophobic high-purity mesoporous silica product is obtained. This invention realizes the transformation from industrial waste to high-performance composite materials, with a yield of mesoporous silica ≥70%, purity ≥99.5%, and activation index ≥95%.

The embodiment of the application discloses an ion exchange membrane and a preparation method and application thereof, and an organic electrosynthesis device. The ion exchange membrane comprises a base film and a functional layer, the functional layer is loaded on the base film, and the material of the functional layer comprises two-dimensional nanomaterials covalently modified with organic molecular fragments, the organic molecular fragments are used for regulating the interlayer spacing of the two-dimensional nanomaterials, and the interlayer spacing of the two-dimensional nanomaterials covalently modified with the organic molecular fragments in the functional layer is 0.8 nm to 1.5 nm. The ion exchange membrane comprises the base film and the functional layer, and the material of the functional layer comprises the two-dimensional nanomaterials covalently modified with the organic molecular fragments. By covalently modifying the two-dimensional nanomaterials with the organic molecular fragments, strong interaction force is introduced between the nanosheet layers of the two-dimensional nanomaterials, the interlayer spacing of the nanosheet layers is effectively 'anchored', and when the ion exchange membrane is soaked in a solvent, the risk that a large amount of solvent enters the interlayer of the nanosheet layers and causes serious swelling of the ion exchange membrane can be reduced.

The invention is applicable to the technical field of nucleic acid extraction, and provides a kit and a method for extracting plant RNA (Ribonucleic Acid) of polysaccharide and polyphenol based on magnetic beads, the kit comprises a lysis solution, a composite remover, a functionalized magnetic bead suspension, a stepped washing solution and an eluent; the surface of the functionalized magnetic bead is covalently modified with a poly (N-isopropylacrylamide-co-vinyl imidazole) copolymer brush (having RNA adsorption and temperature / pH response functions) and a phenylboronic acid (PBA) functional group (specifically capturing polyphenol); the pH value of the matched second washing liquid is controlled to be 5.8-6.8, reversible bond breakage of PBA and polyphenol can be triggered, and selective removal of polyphenol is achieved; meanwhile, the lysate and the composite remover have a synergistic effect, polyphenol oxidation is inhibited from the source, and polysaccharide is precipitated; according to the method disclosed by the invention, the problems of low purity and poor yield in extraction of the RNA of the high-polysaccharide polyphenol plant are solved through a synergistic strategy of'source inhibition-synchronous capture-accurate sorting '.

The invention provides a bone targeting nucleic acid drug delivery system and a preparation method and application thereof, and is characterized in that active components of the bone targeting nucleic acid drug delivery system comprise strong electropositive lactoferrin nanoparticles for loading nucleic acid, bone targeting molecules and a nucleic acid drug, according to the nucleic acid-loaded strong electropositive lactoferrin nanoparticle, the surface of a lactoferrin nanoparticle is covalently modified with a polymerizable acryloyl group, the bone targeting molecule is alendronate, and the surface of the alendronate is covalently modified with a polymerizable acryloyl group. The nucleic acid medicine comprises one or more of small interfering ribonucleic acid, micro ribonucleic acid and nucleic acid aptamers with a bone resorption inhibiting function. The bone targeting nucleic acid drug delivery system is prepared by an in-situ free radical polymerization method. The prepared bone targeted delivery system can be efficiently delivered to bone tissue in a targeted mode, the function of osteoclasts can be regulated and controlled, the osteoinflammation microenvironment is improved by regulating immune balance, bone regeneration of osteoporosis diseases is effectively promoted, and the bone targeted delivery system can be widely applied to treatment of orthopedic diseases.

The invention relates to the technical field of biomedicine nanotechnology, and discloses a hydrogen sulfide donor delivery system for spinal cord injury and blood spinal cord barrier repair as well as a preparation method and application of the hydrogen sulfide donor delivery system. The delivery system takes mesoporous polydopamine nanoparticles MPDA as a core carrier, the surface of the MPDA is covalently modified with targeted polypeptide c (RGDyK), a hydrogen sulfide donor SPRC is loaded in mesopores of the MPDA, and SPRC-coated MPDA-RGD composite nanoparticles are formed. The application provides an innovative solution for targeted nano treatment of spinal cord injury, and has a wide clinical transformation prospect.

The present invention relates to a system for modifying a target biomolecule associated with endogenous intracellular condensates formed by an intracellular protein containing an intrinsically disordered region (IDR), or a system for modifying the chemical or physical properties of endogenous intracellular condensates. The system of the present invention comprises, as its minimum components, an IDR polypeptide sequence tract containing an intrinsically disordered region of an intracellular protein and an effector polypeptide domain capable of covalently modifying the target biomolecule.