The invention discloses an efficient preparation method of wheat
bran feruloyl Arabic
xylooligosaccharide, and belongs to the technical field of
oligosaccharide preparation. Adding water and high-temperature
amylase into the wheat
bran to perform primary enzymolysis, adding
alkaline protease to perform secondary enzymolysis, and performing
enzyme deactivation,
filtration,
water washing,
drying and primary
superfine grinding; adding water to adjust to 30-45wt%, carrying out
extrusion puffing wall-breaking treatment,
cutting into sections,
drying, and carrying out secondary
superfine grinding; adding water, uniformly mixing into paste, and performing high-pressure treatment to obtain wheat
bran paste; performing
microwave treatment, adding water, ligninase,
cellulase and incision beta-1, 4-
xylanase for enzymolysis, and performing
enzyme deactivation to obtain a wheat bran suspension; centrifuging, taking supernate, adsorbing the supernate through macroporous adsorption resin Amberlite XAD-2 at the flow rate of 1-1.5 mL / min, and eluting; and carrying out vacuum concentration on the eluent to obtain an extract, washing with water, and freeze-
drying to obtain the feruloyl arabic
xylooligosaccharide. The green and efficient preparation technology of the feruloyl Arabic
xylooligosaccharide is established by physical means such as
extrusion puffing,
superfine grinding, high-
pressure cooking and
microwave and in combination with multi-
enzyme synergistic enzymolysis.