The invention discloses an ultrasonic and acoustic guided automatic assisted-type epidural puncture assistance system, which is composed of two parts, namely an ultrasonic and acoustic guided automatic assisted-type epidural puncture apparatus and an assisted puncture and control handle, wherein the ultrasonic and acoustic guided automatic assisted-type epidural puncture apparatus is composed of an ultrasonic probe interface, an injection pump component, a pressure sensor, a pressure taking device, a box compartment, a box compartment snap, a loudspeaker, a connecting pipeline, a touch display screen and a circuit module; the assisted puncture and control handle is composed of a disposable positioning mark sliding displacing and supporting elastic sheet, a puncture needle component, a driving shaft linear stepping motor driver, a puncture needle displacement sensor, a rotary switch, a lever type switch, a dual-axis tilt sensor, a rechargeable battery pack, an ultrasonic probe and a connecting cable as well as a connecting pipeline, an injection pump, a pressure sensor and a puncture needle component; and the ultrasonic probe is connected to the dual-axis tilt sensor and the ultrasonic probe interface by virtue of cable lines. With the application of the assistance system provided by the invention, puncture accuracy can be enhanced and complications caused by failure can be reduced.