43 results about "Dicofol" patented technology

The invention provides a preparation method and application of a graphene / carbon nano-tube composite. The preparation method comprises the following steps: oxidizing graphene powder and a multiwalled carbon nano-tube together to obtain graphene oxide and an oxidized carbon nano-tube; carrying out ultrasonic peeling and dispersion on the graphene oxide and the oxidized carbon nano-tube; carrying out modification connection and reduction on the graphene oxide and the oxidized carbon nano-tube with ammonia water and ethylenediamine; and filtering, washing and drying a product after reaction to obtain the graphene / carbon nano-tube composite. The preparation method is simple and convenient, and reaction conditions are mild; in the preparation method, the graphene is connected with the carbon nano-tube through covalent modification, and the carbon nano-tube is connected between graphene slice layers. The graphene / carbon nano-tube composite prepared by the method has a fluffy and porous three-dimensional skeleton structure, and can be used for extracting and detecting melamine, clenbuterol, sulfadimidine sodium, indoleacetic acid, bambuterol, clorprenaline, dicofol, 2,2-bis(4-chlorophenyl)-1,1-dichloroethane or chlorofluoro thiochromanone.

The miticide and pesticide composition containing dimethyl disulfide as one kind of pesticide consists of dimethyl disulfide 0.1-15 wt%, emulsifier 3-20 wt%, and solvent 65-95 wt%; or consists of dimethyl disulfide 0.1-15 wt%, effective component A 0.1-40 wt%, emulsifier 5.0-20 wt%, and solvent 40-90 wt%. The effective component A is at least one of abamectin, pyridaben, diafenthiron, propargite and dicofol; the emulsifier is calcium alkyl benzene sulfonate, polyoxyethylene fatty ester, etc; and the solvent is biodiesel oil, xylene, methanol, etc. The miticide and pesticide composition has the features of high safety, low toxicity, less residue, low cost, high efficiency and broad pesticidal spectrum, and may be used in preventing and controlling mites of crop.

The invention discloses a method for preparing a hapten for detecting dicofol and its application in time-resolved fluorescent test strips and colloidal gold immunochromatographic test strips. The time-resolved fluorescent test strip and the colloidal gold immunochromatographic test strip of the present invention have high sensitivity, high accuracy, high precision, low cost, simple operation, short detection time, suitable for use by various units, simple storage, and long shelf life It has the advantage of being long, and can quickly detect a large number of samples at the same time, and can realize high-throughput rapid detection on site. The time-resolved fluorescent test strip and the colloidal gold immunochromatographic test strip provided by the invention are suitable for determining the residual amount of dicofol in samples such as tea, cabbage, rice, vegetable oil, fish meat and shrimp meat.

The invention discloses a fast detection method for residual dicofol in tea leaves. The fast detection method is characterized in that one-step purification of tea-leaf extracting solution is realized by a high-efficiency sulfonation small column; the purified extracting solution is concentrated, the dicofol is degraded to generate trichloromethane under certain condition and forms a red compound with pyridine, then comparison with standard colorimetric solution or a colorimetric card is made to judge that whether the dicofol exists or not and whether the content of the dicofol exceeds the standard or not. The fast detection method disclosed by the invention has the advantages of simplicity in operation, short consumed time, high sensitivity and low cost, and the detection limit can reach 0.5mg / kg.

The invention provides an acaricide-contained ultra-low-volume composite reagent which is prepared into the form of a static oiling agent and consists of an active ingredient, a conductive agent, a complex solubilizer and a solvent, wherein the active ingredient comprises one type or combination of any two types of the following components: kelthane, pyridaben, propargite and hexythiazox and accounts for 0.1%-73% of the total weight of the composite. The static oiling agent has convenient processing, low cost, good pesticide effect on preventing cotton leaf mites, long continuing efficacy, and work efficiency, thus being capable of reducing environment pollution, improving utilization ratio of pesticides and decreasing usage amount of pesticides.

Presently, most of acaricides in China and abroad are old products such as dicofol of diphenylcarbinol, propargite of sulfurous esters and isocarbophos of organic phosphorous. Most of the old products confront with high resistance due to being used for a long period and the old products are effective only by using in a large dosage; furthermore, the residence time of the old products is long and residue of dicofol is found in crops even one year after the dicofol is applied; isocarbophos is a highly toxic pesticide which is prohibited in the production of green foods, and in most regions where isocarbophos is used for too long time, strong resistance to isocarbophos is generated. Therefore, the old products can not meet the market demands and a novel acarcide is in urgent needed to solve the problems. Aiming at the functions of acarcide, researchers in our company develops a novel compound acarcide - fenbutatin oxide and pyridaben cream which is effective to red spider mites of orange trees and has the advantages of strong killing ability to prosopon acarid, nymphs and young acarid and acarid eggs, long effective period, no temperature influence, good environmental compatibility and safety to natural enemies.

The invention discloses a pesticide special for a mango tree and a preparation method thereof. The pesticide is composed of the following formula components in parts by mass: 20 to 23 parts of carbendazim wettable powder, 17 to 19 parts of polyoxins, 15 to 19 parts of agricultural streptomycin, 13 to 16 parts of holly powder, 10 to 12 parts of sulfur powder, 8 to 11 parts of purslane powder, 6 to 9 parts of fenitrothion, 5 to 8 parts of isoprocarb, 4 to 6 parts of dicofol, 2 to 3 parts of folpet, 3-5 parts of mint powder, 2 to 6 parts of methomyl emulsifiable concentrates, 1 to 3 parts of metrozine, 1 to 2 parts of formalin, 2 to 4 parts of trifmine and 20 to 30 parts of water. The pesticide disclosed by the invention is stable, durable and efficient in effect, and can be used to control various diseases and pests to promote the healthy growth of the mango tree and improve the yield and quality of the mango tree.

The invention discloses a pretreatment method for a finished tea sample for dicofol pesticide residue detection. The pretreatment method comprises the following steps: adding water to moisten a crushed and dried finished tea sample, further adding a dicofol molecularly imprinted polymer, grinding, uniformly mixing, adding into a chromatographic column which is prefilled with the dicofol molecularly imprinted polymer, washing with deionized water, further eluting by using a mixed solvent composed of acetonitrile and glacial acetic acid and taken as an eluent, collecting the eluting liquid, concentrating and drying the eluting liquid, and dissolving and making the volume constant by using n-hexane. By adopting the pretreatment method, a simple grinding technique can be adopted, residual pesticides in the tea sample can be sufficiently adsorbed and extracted while cells of the tea sample are crushed, a great amount or harmful organic solvents are not used, and the analysis and detection cost can be greatly reduced; meanwhile as the dicofol molecularly imprinted polymer is taken as the adsorbent, the pretreatment method has the characteristics of high extraction efficiency on dicofol, good separation effect, small interference, small loss, accurate result and good recyclability.

At present, most of the acaricides produced domestically or abroad are old varieties which mainly comprises propargite of sulfurous ester, kelthane of diphenyl carbinol, azocyclotin and fenbutatin xoide of organotin, pyridaben of heterocyclic compound and isocarbophos of organophosphorus, etc. Due to being used for a long time, the old varieties generate serious drug fastness in most regions and can be effective when being used in high dose. Therefore, the old varieties cannot satisfy the demands of market, and a new acaricide is needed to solve the problem. By aiming at the action of acaricides and combining the characteristics thereof, a novel acaricide of phenoxy pyrazoline esters is developed by the researchers of our company, that is fenpyroximate, which has the action of contact poisoning, and special effects on the egg, young acarids, nymph, adult acarids of acarids in every development phase, and can simultaneously and effectively treat plant diseases and insect pests such as diamondback moth, prodenia litura, Chilo suppressalis, rice fulgorid, barley powdery mildew, oat crown rust, and downy mildew of cucurbits, etc.

The invention discloses a dicofol degrading bacterium and a soil restoration application. The dicofol degrading bacterium provided by the invention is bacterial strain DCF-7 of pseudomonas putida, the bacterium can completely degrade 200mg / L of dicofol in 7days under an aerobic condition, the bacterial strain DCF-7 is sensitive to gentamycin, neomycin and tetracycline, and presents tolerability to 13 types of heavy metals; the dicofol degrading bacterium has the capability for degrading dicofol with high efficiency under actual soil environment, can substantially shorten the timing for degrading dicofol, and has significance and value of practical application.

The invention discloses dicofol hapten, artificial antigen and antibody, and a synthesis method and an application thereof. The invention, for the first time, discloses a dicofol hapten in a novel structure, which has high similarity as a target to-be-test substance, dicofol, wherein the characteristic structure of the dicofol is maintained as most as possible. An amino group is introduced to theposition of phenylchlorine group of the dicofol, so that the antigenic determinant of the hapten can be exposed better, the immunogenicity of the dicofol hapten is significantly enhanced, and the dicofol hapten has an amino group that can be coupled with a carrier protein. After the coupling with the carrier protein, a dicofol immunizing antigen can stimulate the immune response of animals betterto form an antibody having stronger specificity and higher sensitivity when being used for immunizing animals. Indirect competitive ELISA detects proves that the IC 50 of the dicofol hapten is 23.5 [mu]g / L. The result lays the foundation of establishing various immunoassay methods for the dicofol.

The invention belongs to the technical field of agriculture product storage, and particularly relates to a method for storing triennial daffodil bulbs. The method includes the steps of screening the bulbs, soaking the bulbs in a pesticide solution, conserving, drying and storing the bulbs, soaking the bulbs in a pesticide solution composed of a formaldehyde solution, a dicofol emulsifiable concentrate, thiophanate methyl wettable powder and captan wettable powder, and evenly mixing edible fungi waste with domestic sludge in proportion to form paste material as bulb conservation material wrapping base parts and lateral buds of the whole bulbs. Thus, the phenomenon that great soil loss and soil fertility drop are caused when soil is used as the bulb conservation material can be avoided, the phenomenon that the bulbs and the warehouse environment are contaminated by pesticides when a lot of pesticides are sprayed in warehouses can be avoided as well, and the method has remarkable ecological environment protection benefits.

The invention relates to a method of simultaneously detecting dicofol and pyrethroid pesticides in a food. The method comprises the following steps of: making a standard curve; performing ultrasonic centrifugal extraction on a sample with an extraction liquid to obtain a supernatant liquid; purifying the supernatant liquid by using a modified multi-walled carbon nanotube to obtain a sample purifying liquid; and performing gas chromatograph-mass spectrum assay on the sample purifying liquid. By taking the modified multi-walled carbon nanotube as a purifying adsorbent, an improved QuEChERS method and a gas chromatograph-mass spectrometer are combined and applied to simultaneously detecting dicofol and pyrethroid pesticides in apples, a rice, bananas, citrus, pericarpium citri reticulatae and a pericarpium citri reticulatae Pu'er tea sample. The method is simple to operate, high in accuracy and good in stability and meets the requirement of trace analysis.

The invention relates to a method for controlling red spiders in succulent plants. The method includes the following steps that a seedling with a growing point is extracted and cut into a seedling culture plate containing cultivation soil, the periphery of the seedling is covered with straw, the cultivation time is 35 days, and watering is conducted on the seedling with decomposed chicken manure mixing water in the period; when the seedling in the seedling culture plate grows to 2-3 cm, the seedling is transplanted into a basin for cultivation, and it takes 70 days; when the plant grows to 5-7 cm and is plump, the plant is outplanted; the humidity of the basin is increased in the period, meanwhile, a preventive liquid agent is prepared from, by weight, 5 parts of abamectin, 15 parts of dicofol and the balance of water, wherein the abamectin and the dicofol are mixed and then water dilution of 1000 times is conducted, and spraying is conducted on the seedling with the preventive liquid agent. Compared with the prior art, according to the method, 5 parts of abamectin and 15 parts of dicofol are mixed and then the water dilution of 1000 times is conducted, and spraying is conducted on the seedling with the preventive liquid agent obtained, so that red spiders are effectively controlled, and the growth of the succulent plants is accelerated.

The invention provides a method for controlling red spiders of apple trees. The method comprises the following steps: from March to April, after orchard cleaning in spring, when seedlings of grass emerge, dicofol with the mass fraction of 20% is diluted by 1,000 times with water, the diluent is used for spraying to kill the red spiders coming out of hibernation on the ground and foraging on grass twice, and the time interval of the twice spray-killing is 4-6 days; from the last ten days of May to the first ten days of June, after spring shoots of the apple trees stop growing, dicofol and avermectin pyridaben of which the mass fractions are both 20% are diluted by 1,000 times with water and prepared at the volume ratio of 1:1 to spray-skill larvae, nymphs, adults and eggs of red spiders on leaves of the apple trees twice, and the time interval of the twice spray-killing is 4-6 days. The method is simple and feasible, and achieves the purposes of cost decreasing and effectiveness increasing. Compared with a conventional method, the method provided by the invention has the advantages that the labor amount is reduced by 60%, the chemical cost is reduced by 50-60%, nearly 100% of red spiders are killed, the apple trees can be free from red spider harm in the whole year, and overwintering eggs of the red spiders from the last ten days of August to the first ten days of September are prevented.

The invention discloses a special insecticide special for piercing-sucking mouthpart insects and a preparation method for the insecticide. The insecticide consists of the following components in parts by mass: 20 to 30 parts of dichlorvos, 18 to 21 parts of omethoate, 15 to 17 parts of fenitrothion, 10 to 13 parts of decis, 11 to 12 parts of supracide, 7 to 9 parts of dicofol, 8 to 10 parts of anaphicide, 5 to 7 parts of dithane, 3 to 5 parts of octyl mercaptan, 5 to 8 parts of imidacloprid, 3 to 5 parts of Lvling, 5 to 7 parts of pymetrozine, 1 to 3 parts of java paecilomyces, 2 to 4 parts of beta-cypermethrin and 20 to 30 parts of water. The special insecticide for the piercing-sucking mouthpart insects has good stomach poisoning and contact effects, superstrong uptake and translocation performance and stable, persistent and high efficacy.

The invention discloses a quick detection method of residual quantity of dicofol in tea leaves and a detection kit therefor. The detection method includes the steps of: 1) preparing a plurality of n-hexane solutions of the dicofol with successively different concentrations to obtain standard solutions, thereby obtaining standard color developing solutions with certain color gradient and known content of the dicofol; 2) weighing a proper amount of a tea leaf sample to prepare a sample treatment solution; 3) adding a proper amount of sulfuric acid to the sample treatment solution, centrifuging the sample treatment solution to obtain a supernatant, adding a sodium hydroxide solution and pyridine, uniformly mixing the mixture and heating the mixture in water bath to obtain a colored sample color developing solution; and 4) comparing the colored sample color developing solution with the standard color developing solutions to obtain the content of the dicofol in the tea leaf sample. The method can quickly and easily detect the residual quantity of the dicofol in tea leaves, has high sensitivity and is suitable for unprofessional operators in in-situ quick detection.

The invention provides an ultra-low-volume composite reagent containing biological source, which is characterized by being prepared into the form of static oil solution of pesticide and consisting of active ingredients, a conductive agent, a cosolvent and a solvent, wherein the active ingredient I is abamectin, while the active ingredient II is any one of the following: dicofol, pyridaben, propargite and hexythiazox; and the active ingredients account for 0.1%-60% of the total weight of the composite. The static oil solution of pesticide has processing convenience, low cost, good efficacy on preventing and controlling cotton leaf mites, long continuing efficacy and work efficiency, thus being capable reducing environment pollution, improving utilization ratio of pesticides and decreasing usage amount of pesticides.

The invention discloses a technical method for producing bamboo fungus by tea tree residues. The technical method is characterized by comprising the following steps of: utilizing straws and cow dung as a base material, adding pig manure, duck manure, peanut bran, urea, superphosphate, gypsum powder, calcium superphosphate and biogas liquid, stirring uniformly, spreading on the straw surfaces or soil of a mushroom bed, additionally spraying carbendazim, dicofol, dichlorvos and formalin for disinfection, and sterilization and deinsectization to achieve fast growth and 11kg / m<2> of the yield of the bamboo fungus. The technical method disclosed by the invention has the advantages that the yield is high, the quality is good, the mouthfeel is good, the wastes can be utilized, the effect is fast, the income is large, and the yield and the income are increased for farmers; and the bamboo fungus is produced by utilizing the resource of the tea tree residues, so that large economic benefit and social benefit can be generated, the income of the farmers is increased and the wastes also can become into valuables simultaneously.

The invention belongs to the technical field of pesticide preparations, and in particular relates to a mite killing method for safely controlling parasitic mites on osmia excavata alfken. The method for safely controlling the parasitic mites on the osmia excavata alfken comprises the step of exposing osmia pupaes and / or bee tubes containing the parasitic mites to a mite-killing preparation of effective dosage, wherein the effective components of the mite-killing preparation comprise dicofol, pyridaben and propargite. The mite killing method is developed specially for safely controlling the parasitic mites on the osmia excavata alfken, and although the effective components of the preparation adopted by the method are all common acaricides in the prior art, a great reduction of a toxicity effect of the preparation on the osmia and the osmia pupaes is surprisingly discovered on the basis of further improving the specificity for the parasitic mites on the osmia excavata alfken after the preparation is subjected to matching and mutual influence, so the method is more suitable for safely controlling the parasitic mites on the osmia excavata alfken, and an unexpected technical effect is achieved.

The invention discloses a test strip and a method for detecting dicofol. The test strip comprises a sample absorption pad, a conjugate release pad, a reaction film, a water absorption pad and a bottomplate, wherein the reaction film is provided with a detection line coated with a dicofol hapten-carrier protein conjugate and a quality control line coated with a goat anti-mouse antibody, and the conjugate release pad is sprayed with a dicofol monoclonal antibody-colloidal gold marker. The invention further provides the method for detecting the dicofol in a fruit sample by using the test strip.The test strip provided by the invention has the advantages of simple in operation, high sensitivity, high detection speed, low cost, suitability for large-batch sample screening and the like, and canmeet the requirements of food supervision departments in China for carrying out on-site monitoring and detection work.

The invention discloses a hybridoma cell strain capable of secreting a dicofol monoclonal antibody and application of the hybridoma cell strain, and belongs to the technical field of immunodetection. The invention discloses a hybridoma cell strain JHJ capable of secreting a dicofol monoclonal antibody. The hybridoma cell strain JHJ is collected in the China General Microbiological Culture Collection Center (CGMCC) and is classified and named as the monoclonal cell strain JHJ with the collection date of May 13, 2021 and the collection number of CGMCC No.22329. The hybridoma cell strain is obtained by immunizing a BALB / c mouse with a dicofol complete antigen, taking splenocytes of the mouse with high titer and good inhibition, fusing with myeloma cells through a PEG method, and carrying out screening and three times of subcloning through an indirect competitive enzyme-linked immunosorbent assay. The monoclonal antibody secreted by the cell strain has good specificity and detection sensitivity (IC50 value is 2ng / mL) to dicofol, provides a raw material for immunodetection of dicofol residues in food, and has a practical application value.

The invention discloses a dicofol hapten, a dicofol artificial antigen, a dicofol antibody, and a preparation method and application of the dicofol antibody. According to the invention, dicofol haptenprovided by the invention retains the characteristic structure of dicofol to the greatest extent, so that the immunogenicity of the dicofol hapten is obviously enhanced; the dicofol hapten has carboxyl capable of being coupled with a carrier protein, and is coupled with the carrier protein to obtain a dicofol artificial antigen, and the dicofol artificial antigen immunizes animals, so that generation of antibody with strong specificity and high sensitivity by stimulating animal immune response is facilitated; and the detection results show that the sensitivity of the dicofol antibody can reach 0.1 [mu]g / L, the cross reaction rate of the dicofol antibody and other organochlorine pesticides is low, and a foundation is provided for establishing various immunoassay methods of dicofol subsequently.

A bacterial preparation for degradating the residual agricultural chemical on agricultural crops by directly applying it on the growing crops is the photosynthetic bacteria. It is prepared through inoculating culture to culture box, culturing under light irradiation, inoculating to productive container, and culturing under light irradiation. Its advantage is high degradation effect.

The invention belongs to the technical field of standard solutions, and particularly relates to a preparation method of a dicofol standard solution, which comprises the following steps: calculating the amount of the required standard substance dicofol and the amount of the required solvent n-hexane according to the determined concentration C0 and volume V0 of the dicofol standard solution, and weighing the dicofol; then dissolving with methylbenzene, and then adding normal hexane to a constant volume to obtain a normal hexane solution of dicofol; determining the concentration value of the dicofol in the solution, performing parallel determination for three times, taking the average value C1 of the concentrations, and taking the C1 in the measurement uncertainty range as the detection concentration of the dicofol standard solution, wherein the normal hexane solution of the dicofol in accordance with the measurement uncertainty range is the dicofol standard solution. By adding methylbenzene, the stability of the dicofol solution standard substance is better, and the problem that the dicofol solution standard substance in normal hexane is unstable in the placement process is solved.

The invention relates to a preparing method for a degradation agent for biological pesticide residue and belongs to the technical field of preparation of the degradation agents. The method comprises: firstly cultivating aegilops tauschii by using self-made cultivating soil, spraying pesticide and irradiating with X rays in a cultivating process, collecting aegilops tauschii straws on the surface of the cultivating soil, mixing the collected straws with water and then juicing, collecting a juiced solution and juiced residue, pouring the juiced solution into the cultivating soil containing aegilops tauschii roots for fermentation, mixing a fermented product with a glucose solution to obtain a fermentation mixture and filtering the fermentation mixture, collecting supernatant, carbonizing the juiced residue and then soaking a carrier in the glucose solution, and mixing the carrier taken out of the glucose solution with substances such as the supernatant and sodium alginate. The degradation agent for biological pesticide residue prepared through the preparing method provided by the invention is suitable for crops such as wheat, rice, soybean, corn, Chinese cabbage and leek, and is effective for pesticides such as fenpropathrin, dimehypo, carbofuran, carbofuran, dicofol, triazolone and methamidophos and is fast in degradation speed, and can be totally degraded in 9-10 days.

The present invention provides for crystalline Form B1 of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenyl-methyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt, crystalline Form B2 of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt, a novel process for the preparation of crystalline Form B of N-(3-carboxyl-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester sodium salt. The present invention also provides for crystalline Form B of (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-Amine disodium salt, crystalline Form P of (S)—N-(1-Carboxy-2-Methyl-Prop-1-yl)-N-Pentanoyl-N-[2′-(1H-Tetrazol-5-yl)-Biphenyl-4-yl-Methyl]-Amine disodium salt and processes for their preparation thereof. The present invention further provides an industrial method production of Amorphous Form of Sacubitril valsartan trisodium. The present invention also provides Sfor amorphous solid dispersions of Sacubitril valsartan trisodium with excipients.

The invention relates to a method for determining the residual quantity of dicofol in foods, and concretely relates to a method for determining the residual quantity of dicofol in eel and eel products. The method comprises the following steps: carrying out vortex extraction of dicofol alkalization products through using acetone-petroleum ether (1+1V+V), carrying out rotary evaporation of the obtained extract solution at 40DEG C to near dryness, adding n-hexane for dissolving residues, sulfonating by concentrated sulfuric acid to remove lipid impurities and possible-existence 4,4'-DBP in the extract solution, alkalizing dicofol to 4,4'-DBP by using a 10mol / L potassium hydroxide solution and anhydrous ethanol, and determining with a gas chromatograph provided with a micro-electronic capture detector. An external standard method is used for quantification, and when the addition concentration is 0.01-0.05mg / kg, the recovery rate is in a range of 72.2-118.3%, the relative standard deviation is less than 12.6%, and methodologic indexes satisfy the pesticide residual detection requirement. The determination method solves a problem of the unstable dicofol determination result, and eliminates a severe interference problem of complex matrixes.

The invention discloses a dicofol degrading bacterium and a soil restoration application. The dicofol degrading bacterium provided by the invention is bacterial strain DCF-7 of pseudomonas putida, the bacterium can completely degrade 200mg / L of dicofol in 7days under an aerobic condition, the bacterial strain DCF-7 is sensitive to gentamycin, neomycin and tetracycline, and presents tolerability to 13 types of heavy metals; the dicofol degrading bacterium has the capability for degrading dicofol with high efficiency under actual soil environment, can substantially shorten the timing for degrading dicofol, and has significance and value of practical application.

The invention discloses a detection kit and a method for dicofol. The principle that the activity of lipase can be increased along with the increase of the concentration of the dicofol is utilized; whether the dicofol exists or not is detected by detecting the lipase activity, so that the dosage of an organic solvent is reduced, the detection limit is improved, strong acid and an organic solvent with foul smell are prevented from being used, the operation process is simplified, the detection efficiency is improved, and the method is suitable for field operation and rapid detection.