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Test strip and method for detecting dicofol

A technology of dicofol and test strips, which is applied in the field of test strips for detecting dicofol, can solve the problems that it is difficult to meet the rapid detection of a large number of samples and on-site samples, the processing is cumbersome and time-consuming, and the detection cost is high. It achieves short detection time, easy promotion and use, The effect of high sensitivity

Pending Publication Date: 2020-06-16
BEIJING KWINBON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods must be operated under laboratory conditions. The sample pretreatment is cumbersome and time-consuming, and expensive instruments and equipment are required. The detection cost is high, time-consuming, and the operation is complicated. There are great limitations in the actual application process, and it is difficult to meet The need for rapid detection of a large number of samples and on-site samples

Method used

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  • Test strip and method for detecting dicofol
  • Test strip and method for detecting dicofol
  • Test strip and method for detecting dicofol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Preparation of test strips for detecting dicofol

[0041] The preparation method of this test strip mainly comprises the following steps:

[0042] 1) Prepare a conjugate release pad sprayed with dicofol monoclonal antibody-colloidal gold marker;

[0043] 2) preparing a reaction membrane with a detection line coated with a dicofol hapten-carrier protein conjugate and a quality control line coated with a goat anti-mouse anti-antibody;

[0044] 3) Assemble the conjugate release pad prepared in 1) and 2), the reaction membrane, the sample absorption pad, the water absorption pad and the bottom plate into a test strip.

[0045] The following step-by-step detailed description:

[0046] 1. Synthesis of dicofol hapten (synthetic route sees attached figure 1 )

[0047] Take 1.7g of o-chlorophenylacetic acid and add 100mL of carbon tetrachloride to dissolve, add 1.12g of chlorobenzene, stir well, add 1.47g of chloral, cool to 10-15°C, add dropwise 10mL of 10% sulfu...

Embodiment 2

[0079] The detection of dicofol in the fruit of embodiment 2

[0080] 1. Sample pretreatment

[0081] Before the test, remove the mud and chop the sample into pieces or small pieces smaller than 1cm; weigh (2.00±0.05)g of the chopped sample into a 15mL centrifuge tube, add 4mL of phosphate buffer, cover it, and shake it manually 30s, let stand for 1min, and take the supernatant as the sample solution to be tested.

[0082] 2. Test with test strips

[0083] Use a micropipette to draw 70 μL of the sample solution to be tested and drop it vertically into the sample hole; start timing when the liquid flows, react for 10 minutes, and judge the result.

[0084] 3. Analyze test results

[0085] Negative (-): The color of the T line is darker than that of the C line or consistent with the color of the C line, indicating that the concentration of dicofol in the sample is lower than the detection limit, such as image 3 a, 3b.

[0086] Positive (+): T-line color is lighter than C-li...

Embodiment 3

[0088] Example 3 sample detection example

[0089] 1. Detection limit test

[0090] Take blank apple and pear samples, add dicofol to them to the final concentration of 0.5mg / kg, 1.0mg / kg, and 2.0mg / kg, respectively, take test strips for detection, and repeat the measurement three times for each sample.

[0091] When using test strips to detect apple and pear samples, when there is no dicofol and its concentration is 0.5mg / kg, the test strip shows that the T line color is darker than the C line color or is consistent with the C line color, Negative; when the added concentration of dicofol is 1.0mg / kg, 2.0mg / kg, the test strip shows that the color of the T line is lighter than that of the C line or the T line is not colored, which is positive, indicating that the test paper The detection limit of dicofol in fruit is 1.0mg / kg.

[0092] 2. False positive rate and false negative rate test

[0093] Take 20 blank apple and pear samples and 20 positive apple and pear samples added...

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Abstract

The invention discloses a test strip and a method for detecting dicofol. The test strip comprises a sample absorption pad, a conjugate release pad, a reaction film, a water absorption pad and a bottomplate, wherein the reaction film is provided with a detection line coated with a dicofol hapten-carrier protein conjugate and a quality control line coated with a goat anti-mouse antibody, and the conjugate release pad is sprayed with a dicofol monoclonal antibody-colloidal gold marker. The invention further provides the method for detecting the dicofol in a fruit sample by using the test strip.The test strip provided by the invention has the advantages of simple in operation, high sensitivity, high detection speed, low cost, suitability for large-batch sample screening and the like, and canmeet the requirements of food supervision departments in China for carrying out on-site monitoring and detection work.

Description

technical field [0001] The invention relates to a test strip and a method for detecting dicofol, in particular to a colloidal gold test strip for detecting dicofol, which is particularly suitable for detecting dicofol residues in fruits. Background technique [0002] Dicofol (dicofol) is an organochlorine acaricide, which can prevent and control mites on crops such as cotton, fruit trees, and vegetables. It has strong contact and stomach poisoning effects on adults, nymphs, and eggs. long term. Studies have shown that the drug has certain toxicity to humans and animals. The main symptoms of poisoning are headache, dizziness, sweating, chest tightness, mydriasis, blurred vision, nausea, vomiting, and diarrhea. Local exposure can cause contact dermatitis. Dicofol has been banned in my country, and the national standard GB2763-2019 "National Food Safety Standard Maximum Residue Limits of Pesticides in Food" also stipulates that the residue limit of dicofol in fruits is 1mg / kg....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/58G01N33/577G01N33/558G01N33/53
CPCG01N33/587G01N33/577G01N33/558G01N33/5308
Inventor 万宇平王兆芹崔海峰宋灏赵正苗凡静静任西杰郝晓娜
Owner BEIJING KWINBON BIOTECH
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