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Dicofol degrading bacterium and soil restoration application

A technology of dicofol and degrading bacteria, which is applied in the field of biological treatment of environmental pollutants, can solve the problems of entering the soil, harm, pollution, etc., and achieves the effects of slow application effect, good general performance and fast degradation rate

Inactive Publication Date: 2012-10-10
ZHEJIANG FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the utilization rate of pesticides is less than 20%, and the rest all enter the soil, water and atmosphere, causing serious pollution and harm.
However, highly efficient degrading strains are very limited, and the 7-day degradation rate of most strains to 100 mg / L dicofol is less than 88%
In addition, the antibiotic resistance and heavy metal tolerance of the above-mentioned degrading bacteria have not been proven, and there is a certain risk of environmental application

Method used

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  • Dicofol degrading bacterium and soil restoration application
  • Dicofol degrading bacterium and soil restoration application
  • Dicofol degrading bacterium and soil restoration application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Pseudomonas putida Separation of DCF-7 and its ability to degrade dicofol

[0032] The soil near the sewage outlet of Tonglu Pesticide Factory in Zhejiang was collected on site, packed in a 150 mL triangular flask, and quickly brought back to the laboratory. The retrieved samples were placed in dicofol-selective basic inorganic salt liquid medium for acclimation culture. The medium composition is (g / L): MgCl 2 0.2, NH 4 NO 3 1, KH 2 PO 4 2,K 2 HPO 4 7.5, NaCl 1, dicofol 0.15 (pre-dissolved in acetone), pH 6.8, 1 L of deionized water, sterilized by moist heat at 115°C for 30 minutes before use. The culture conditions are: 25°C, 120 r / min shaking culture, and the liquid volume in the container is 1 / 3 of the container volume. Thereafter, the shake flask was kept still for a while every 5 days, the supernatant was discarded, and then an equal amount of freshly prepared selective liquid medium was added and the shaking enrichment culture was contin...

Embodiment 2

[0035] Example 2: Pseudomonas putida Drug resistance experiment of DCF-7

[0036] Tetracycline, gentamicin and neomycin were selected for testing by the filter paper method, and the diameter of the inhibition zone of each antibiotic filter paper (30 μg / piece) on the cultured degrading bacteria DCF-7 plate was used as the sensitivity or resistance basis for judgment. The test results showed that the diameters of the inhibition zone were 24, 28 and 32 mm in sequence, showing moderate, highly and extremely sensitive reactions respectively (the sensitive range is: the moderately sensitive range of tetracycline is 23-25 ​​mm; the highly sensitive range of gentamicin is 26 mm; -30 mm; neomycin extremely sensitive range >30 mm).

[0037] This example shows that when the bacteria are released into the natural environment, super bacteria will not be produced due to the problem of drug resistance (resistance), which provides a safety guarantee for its future practical application. ...

Embodiment 3

[0038] Example 3: Pseudomonas putida Heavy Metal Minimal Inhibitory Concentration (MIC) Test of DCF-7

[0039] The design of the MIC experiment was carried out with reference to the research of Filali et al. (Filali B.K., Taoufik J., Zeroual Y., Dzairi F.Z., Talbi M., Blaghen M. Waste water bacterial isolates resistant to heavy metals and antibiotics. Current Microbiology, 2000, 41: 151-156), pick the same size DCF-7 single colony after activation treatment, put it in the culture test tube, 30 ℃, 150 r / min shaking culture for 48 hours. The MIC value was determined according to the growth of the bacteria, and three replicates were set up in each group to test the tolerance of the bacteria to 13 metal ions. The results are shown in Table 2, the strain has multiple tolerance and strong tolerance.

[0040]

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Abstract

The invention discloses a dicofol degrading bacterium and a soil restoration application. The dicofol degrading bacterium provided by the invention is bacterial strain DCF-7 of pseudomonas putida, the bacterium can completely degrade 200mg / L of dicofol in 7days under an aerobic condition, the bacterial strain DCF-7 is sensitive to gentamycin, neomycin and tetracycline, and presents tolerability to 13 types of heavy metals; the dicofol degrading bacterium has the capability for degrading dicofol with high efficiency under actual soil environment, can substantially shorten the timing for degrading dicofol, and has significance and value of practical application.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollutants, and in particular relates to a bacterium for efficiently degrading dicofol and its application in soil pollution restoration. Background technique [0002] As an important means of agricultural production, pesticides play an extremely important role in protecting agricultural production, improving comprehensive agricultural production capacity, promoting stable grain production and increasing farmers' income. China is not only a big producer of pesticides, but also a big user, with the annual and average usage ranking first in the world. However, the utilization rate of pesticides is less than 20%, and the rest all enter the soil, water and atmosphere, forming serious pollution and harm. Statistics show that the area of ​​farmland polluted by pesticide residues in China is about 15 million hectares, accounting for 10% of the arable area. Nearly half of th...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/40
Inventor 虞方伯管莉菠骆林平单胜道刘畅龙珍
Owner ZHEJIANG FORESTRY UNIVERSITY
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