18 results about "Inhibition zone" patented technology

This invention belongs to the field of biotechnology and discloses a strain of Bacillus amyloliquefaciens ZJ02 that inhibits bacterial growth and reduces ammonia nitrogen. Its accession number is CCTCC NO: M20252399, the accession date is October 30, 2025, and the depositary institution is the China Center for Type Culture Collection, located at Wuhan University, Wuhan, China. ZJ02 exhibits significant antibacterial effects against Escherichia coli and Salmonella, with an inhibition zone size of 18.11 mm against Salmonella (SA) after 24 hours. Compared to other strains, it demonstrates superior ammonia nitrogen degradation under the same conditions, achieving a degradation rate of 76.58%, reaching as high as 87.57% at an initial ammonia nitrogen concentration of 500 mg / L. Furthermore, it possesses excellent ability to reduce ammonia release from animal feces. In ammonia removal tests conducted in pig farm environments, compared to the control group, the NH3 concentration decreased by 60.27% on day 6, significantly outperforming Bacillus subtilis and Bacillus licheniformis. This invention also provides the application of this Bacillus amyloliquefaciens.

The present application relates to the field of microbial agricultural technology, and more particularly to a microbial preparation, a preparation method thereof and application thereof in improving continuous cropping obstacles, and provides a microbial preparation, a preparation method thereof and application thereof, wherein the microbial preparation is prepared by fermentation of Bacillus subtilis, and the fermentation culture solution contains shrimp and crab shells, collagen, sodium chloride, amino acids, carbohydrates and compound fertilizer, so that the preparation can not only inhibit harmful bacteria, but also provide a large amount of nutrients for crops. Experiments show that the microbial preparation provided by the present application can make the inhibition zone of the bacterial agent on pathogenic bacteria reach more than 6 times that of the control group. Field experiments show that the bacterial agent can significantly increase the content of trace elements in soil and improve the diseases of ginseng, panax notoginseng and rehmannia caused by continuous cropping.

The invention relates to a bacteriostatic feed and a preparation method and application thereof, the bacteriostatic feed comprises a basic ration and yeast essence, the content of beta-glucan in the yeast essence is 25-50%, and the relative molecular weight of more than 99% of beta-glucan is 3.0 * 10 < 5 >-1.0 * 10 < 6 >; the content of mannan in the yeast essence is greater than or equal to 20%, and the relative molecular weight of mannan of 99% or above is 8.0 * 10 < 5 >-2.0 * 10 < 6 >. The yeast essence involved in the feed disclosed by the invention is added into the feed as a single substance, the recommended dosage is stable, and the yeast essence shows a relatively good resistance replacement effect in in-vitro inhibition zone simulation and in-vivo test verification of bred animals; a set of efficient and feasible antibiotic substitution scheme can be provided for the animal breeding industry, meanwhile, the defects of antibiotic drug resistance and residues and poor effect and poor stability of current antibiotic substitution products are overcome, and the future prospect is considerable.

This invention relates to the field of automated microbial antimicrobial susceptibility testing technology, specifically to an automatic device for measuring the diameter of inhibition zones. The device includes an image acquisition module, an antimicrobial susceptibility test strip center positioning module, a radial transmittance profile construction module, a diffusion-MIC inversion module, and a diameter output module. This invention reconstructs the traditional inhibition zone measurement paradigm based on image edge detection and geometric circle fitting into a radial profile inversion paradigm based on a physical model of antimicrobial substance diffusion in agar. It constructs a radial transmittance profile and a family of azimuth profiles with the center of the antimicrobial susceptibility test strip as the polar coordinate origin, inversely infers the local diffusion coefficient field, and uses Fick's second law to nonlinearly invert the minimum inhibitory concentration (MIC) radius. Simultaneously, it separates dual-zone resistant subgroups through residual multi-peak detection and achieves dynamic prediction through temporal differential constraints. This invention achieves a measurement standard deviation better than 0.1 mm, supports dual-zone identification and culture endpoint prediction, and is significantly superior to existing automatic measurement schemes based on edge detection.

The invention belongs to the technical field of microorganisms, and relates to an actinomycete strain and application thereof. The actinomycete strain is classified and named as amycolatopsis reevesii NVA54, the Latin name is Amycolatopsis reevesii, the preservation number of the actinomycete strain is CGMCC (China General Microbiological Culture Collection Center) 33989, and the preservation date is March 26, 2025. The invention further discloses a bacteriostatic agent containing the actinomycetes strain and application of the bacteriostatic agent and the actinomycetes strain. The actinomycete strain can obviously inhibit pathogenic bacteria of root rot, an obvious antibacterial zone is formed, and the antibacterial rate is not lower than 87.53%.

The utility model belongs to the technical field of experimental utensils, and discloses a culture dish device for laboratory bacteriostatic activity tests, a perforated plate is mounted in a culture dish base in a penetrating manner, and a culture dish cover is placed on the top of the perforated plate; seven hole forming columns are fixedly installed at the bottom of the punching plate, and two symmetrically-arranged S-shaped components are fixedly installed on the outer side of the punching plate. The perforated plate can be effectively separated by adopting the S-shaped component, so that the solidified culture medium forms uniform sample adding holes, and meanwhile, the pollution risk of the culture medium is reduced; the fixed clamping seat and the positioning groove are adopted to effectively position the position of the sample adding hole to be concentric with the positioning circle, direct measurement and comparison of test effects are facilitated, compared with a traditional puncher, the device is easy to operate, the defects of cracking of a solid culture medium and the like are not easily caused, and the environmental microbial pollution risk in the bacteriostatic experiment process is effectively controlled; and the diameter of the inhibition zone can be directly measured.

The invention discloses an antibacterial peptide fragment intercepted from a mined fly IDGF4 protein and application of the antibacterial peptide fragment in preparation of antibacterial drugs. According to the invention, oligopeptide fragments at different parts in the collected fly IDGF4 protein are screened, three candidate peptides are obtained through chemical synthesis, the antibacterial performance of the candidate peptides is systematically evaluated, antibacterial experiment results show that the oligopeptide with the amino acid sequence shown as SEQ ID No.2 can effectively inhibit the growth of salmonella and staphylococcus aureus under the concentration of 1mg / mL, and the oligopeptide with the amino acid sequence shown as SEQ ID No.2 can effectively inhibit the growth of salmonella and staphylococcus aureus under the concentration of 1mg / mL. The antibacterial effect of the peptide is confirmed in inhibition zone experiments, enzyme-labeled turbidimetry and growth curve monitoring, and the peptide shows stable and remarkable antibacterial activity, which indicates that the peptide can be used as a novel antibacterial molecule. The invention provides a new candidate sequence for developing the antibacterial peptide with high efficiency and low drug resistance risk, and has application potential in the aspect of preparing clinical antibacterial drugs.

Systems, methods of manufacture, and packaging configurations for an antimicrobial orthopedic implant having an antimicrobial coating on the outer surface of the implant including a vaporizable antimicrobial agent in a surface area concentration on the outer surface sufficient to prevent bacterial growth on the orthopedic implant, and provide a clinically effective zone of inhibition around the orthopedic implant. In certain embodiments, a container, a reservoir of the vaporizable antimicrobial agent, and the orthopedic implant are configured to remain thermally stable in a temperature range up to 200 C.

The application discloses an inducing method for improving antibacterial peptide activity of black soldier fly, and belongs to the field of insect biotechnology. The method realizes efficient induction of antibacterial peptide by adding Rhodopseudomonas palustris (RP) in the 5th instar larvae of black soldier fly to reinforce the basic daily ration, and combining ultrasonic reinforcement with short-time heat shock stress treatment. Test data show that the antibacterial peptide crude extract induced by the method has an inhibition zone diameter of 40.48 mm on salmonella. The application solves the problem of large-scale production of antibacterial peptide, balances the immune cost caused by high-intensity induction through reinforcing substrate compensation mechanism, and has the advantages of strong induction activity, stable growth performance, low process cost and the like, and has application prospects in the fields of animal feed additives and biological antibacterial agents.

This invention discloses the application of isocitric acid in the control of bacterial wilt in plants, wherein the isocitric acid is derived from tomato root exudates. The inhibitory effect of isocitric acid on *Ralstonia solanacearum* was evaluated through shake-flask culture experiments and plate inhibition zone experiments, and the control effect of isocitric acid on bacterial wilt was determined using greenhouse pot experiments. The results showed that within the concentration range of 10–200 μM, isocitric acid significantly inhibited the growth of *Ralstonia solanacearum*, forming a distinct inhibition zone. Under greenhouse pot conditions, applying 0.2–0.5 mg / kg of isocitric acid to the soil effectively reduced the incidence of bacterial wilt in tomatoes, with a disease control rate of 21.43%–52.38%. Furthermore, isocitric acid can indirectly inhibit the occurrence of bacterial wilt by influencing the soil bacterial community. The isocitric acid used in this invention is derived from natural plant root exudates, possessing advantages such as environmental friendliness and high safety, and can be used as a novel inhibitor of tomato bacterial wilt, showing promising application prospects in the field of green control of plant diseases.

This invention relates to a gel preparation with pistachio shell extract as the active ingredient which has the effect of inhibiting Staphylococcus aureus bacteria. The gel consists of pistachio shell extract made from a maceration process using 70% alcohol, 5% CMC-Na gel base, 5.0% glycerin humectant, 3.0% propylene glycol, 0.25% methyl paraben preservative and aquades solvent added up to 100%. The gel has antibacterial activity which is characterized by the formation of an inhibition zone against Staphylococcus aureus bacteria. The emergence of this inhibition zone is due to the active compound contained in the gel base diffusing through the media so that it is able to inhibit the growth of Staphylococcus aureus bacteria. The positive control used in this antibacterial test is 1.2% clindamycin phosphate gel.From the results of the antibacterial effectiveness test that has been carried out, it was found that 10% to 25% pistachio shell extract gel can inhibit ≥15mm-24mm of Staphylococcus aureus bacterial growth with Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values ​​of 80% and 100%, respectively.

This invention relates to the field of cultural relic protection technology, specifically to a plant-based antibacterial agent for the prevention and control of microorganisms in earthen sites and its screening method. The antibacterial agent uses citral as its core component and ensures high antibacterial efficiency through a systematic screening method (including microbial identification, inhibition zone testing, MIC / MBC determination, and persistence assessment). Experiments show that citral has strong inhibitory effects on common pathogens in earthen sites (such as Alternaria and Mucor), and a 6% concentration can achieve at least 7 days of sustained antibacterial activity. This invention is green and safe, providing a reliable solution for the prevention and control of microorganisms in earthen sites.

The invention discloses a microbial bactericide and application thereof. The microbial bactericide comprises the following components: a functional microbial complex microbial inoculant, a targeting bacteriophage composition, a pH-responsive nanogel carrier and an auxiliary agent, wherein the functional microorganism complex microbial inoculant comprises a bacterial strain which is separated from deep-sea sediments and screened by a plate confrontation method, and the diameter of an inhibition zone of the bacterial strain on at least one target pathogenic bacterium is greater than or equal to 15mm; the targeted bacteriophage composition comprises at least two lytic bacteriophages, and the lytic bacteriophages are used for respectively and specifically splitting different types of pathogenic bacteria including ralstonia solanacearum, pseudomonas syringae and Erwinia; four mechanisms of competitive occupation, antibacterial substance inhibition, bacteriophage lysis and induction system resistance are integrated, and the limitation of a single action mode is broken through.

This invention relates to the field of microbial technology, specifically to a heat-resistant *Lactaseibacillus paracasei* and its applications. The preservation number for *Lactaseibacillus paracasei* R01NGW is GDMCC No:66026. This invention utilizes gradient high-temperature acclimatization with chicken manure and screening on a selective medium containing polylysine hydrochloride to obtain a strain that is both heat-resistant (survival ≥10 μL at 70℃). 8 This strain exhibits high CFU / mL concentration, strong antibacterial activity (inhibition zone 15mm-20mm), and resistance to gastric acid and bile salts. The screening medium utilizes a triple screening mechanism of "physical + chemical + biological targeted inhibition," achieving a 99.9% inhibition rate against Bacillus. This strain can be used in high-temperature feed processing, silage fermentation, probiotic preparations, and bio-antibacterial applications, providing a safe and efficient solution for the application of lactic acid bacteria in heat-resistant environments.

This invention discloses a biocontrol agent of *Brevibacillus halotolerans* strain Z-9 and its application. *Brevibacillus halotolerans* Z-9, a new strain belonging to the genus *Brevibacillus*, was isolated from the rhizosphere soils of cotton and jujube in Tumushuke and Alar, Xinjiang. Strain Z-9 exhibits inhibition zone diameters of 23.32 mm and 25.68 mm against *Brevibacillus halotolerans*, the causal agent of jujube black spot, and cotton verticillium wilt, respectively. It contains catechol-type siderophores and is negative for isohydroxamic acid-type siderophores. The minimum As / Ar ratio is 0.682±0.021, indicating the highest iron production capacity. Field application of the biocontrol agent prepared using strain Z-9 reduced the disease indices of cotton verticillium wilt and jujube black spot to 7.14 and 8.34, respectively, achieving a control efficacy of 78.07%. This indicates that the strain Brevibacillus halotolerans Z-9 provided by this invention and the biocontrol agent prepared therefrom are highly effective against jujube black spot disease and cotton Verticillium wilt, and have no toxicity or pathogenicity or other side effects. They have broad application value for the biological control of jujube black spot disease and cotton Verticillium wilt.

This invention belongs to the field of gene technology and discloses a bacterial strain antagonistic to konjac soft rot and its application. The *Pseudomonas pallens* strain B07 of this invention exhibits significant antagonistic activity against the pathogen causing konjac soft rot, with an inhibition zone diameter of 1.05±0.16 cm. In pot experiments, it reduced the incidence of konjac soft rot by 33.34% and the disease index by 22.22%. Simultaneously, this strain possesses plant growth-promoting characteristics such as phosphorus solubilization, siderophore production, and nitrogen fixation. It also shows broad-spectrum antagonistic activity against eight plant pathogenic fungi, including konjac white mold and *Amorphophallus bulbifer* leaf spot, with inhibition rates ranging from 44.87% to 60.03%. Whole-genome analysis shows that its genome contains 14 secondary metabolite biosynthesis gene clusters, providing the molecular basis for its antibacterial activity. This strain can be applied directly or prepared as a biocontrol agent for the prevention and control of konjac soft rot. It is environmentally friendly, residue-free, and possesses both disease prevention and growth-promoting effects, making it of significant industrial value in the green control of konjac diseases.

Provided are orthopedic implants and systems and kits containing orthopedic implants that include biodegradable polymer thin films containing an antimicrobial agent, wherein the implants produce an effective zone of inhibition around a periphery of the surface of the implant and do not produce a loss of release torque between interlocking implant components.

The invention relates to the identification of the Populus ciliata tree bark as a natural antioxidant and with potential antimicrobial activities. Crude methanol bark extract and its fractions (ethyl acetate, n-butanol, chloroform and n-hexane) were screened for antioxidant analysis and antimicrobial activities against gram positive (B. subtilis, S. aureus) and gram negative (E. coli, P. aeruginosa and K. pneumoniae) bacteria. Results of DPPH, ABTS and FRAP assays revealed the highest antioxidant potential of n-butanol and ethyl acetate fractions with lower IC50 value as compared to standard ascorbic acid, crude methanol extract and others fractions. Total phenolic content and total flavonoids content were also observed to be higher in n-butanol fraction (317.83±2.95 mg / g gallic acid and 271.26±0.89 mg / g quercetin, respectively) followed by ethyl acetate fraction, than crude methanol extract, chloroform and hexane fractions of bark indicating the highest antioxidant potential of P. ciliata bark extracts. Antibacterial assay also showed higher zone of inhibition of n-butanol and ethyl acetate fractions of P. ciliata bark against gram positive and gram negative bacteria, later on supported by the lower MIC value (62.5µg / ml) of both fractions. Present invention concluded P. ciliata tree bark as higher antioxidant and antibacterial potential agent whose n-butanol and ethyl acetate fraction was observed to be more effective than crude extract alone. This work further includes the extraction and identification of compounds with higher biological activities (antioxidant and antifungal activities) which would be further helpful in decreasing the pressure of pharmaceutical companies on wild herbaceous medicinal plants.