178 results about "Inhibition zone" patented technology

The invention relates to a hyper branched polyamide amine and metal nanometer compound and the preparation method and the application, belonging to the technical field of nanotechnology which is characterized in that: the compound comprises a polymer and metal nanometer particles, the polymer is the hyper branched polyamide amine made of blocking modification the hyper branched polyamide amine of the vinyl-terminated through different small organic molecules with amine, the content of the polymer is 89wt percent to 98wt percent; the metal nanometer particles are golden or silver nanometer particles, the content of the metal nanometer particles is 2wt percent to 11wt percent. During the preparation course, no reducing agent or ultraviolet irradiation is required, the precursor metal compounds are directly added into the hyper branched polyamide amine solution, and the metal nanometer particles with perfect water dispersion is generated only through one-step reaction under ambient temperature, the average particle size of which is one to eight nm, and the size is controllable. The inhibition zone method and the optical density method tested show that: the hyper branched polyamide amine and the metal nanometer particle compound have perfect antibacterial effects to a plurality of bacteria and fungus.

The invention provides composition comprising microcapsules containing biocides for coating material such as paint. The invention describes composition of microcapsules containing biocides, especially Irgarol (algaecide) and Zinc Pyrithione (fungicide) and coating material such as paint to increase the life of coating material from the attack of algae and fungi respectively. The invention demonstrates that microcapsules containing biocide dispersed in paint show 0 to 3% reduction in inhibition zone where as un-encapsulated biocide show 20-22% reduction in inhibition zone when analyzed by filter paper assay thus indicating extended duration of biocidal activity with microencapsulated biocide.

The invention provides a biocontrol bacterial strain for preventing and treating plant diseases and its bacterial agent, belonging to the field of biological control. The strains used are Gram-negative bacteria, identified as Lysobacter enzymogenes, and the strain code is OH11. Strain OH11 has no flagella but has slippage, can produce various extracellular hydrolytic enzymes including chitinase, β-1,3-glucanase and protease, and can effectively inhibit the growth of fungi and bacteria. The antibacterial zone diameters of strain OH11 against Sclerotinia sclerotiorum and Phytophthora capsici were both greater than 22.0 mm; the antagonism against potato ring rot was stronger, and the diameter of the inhibition zone reached 50 mm. The OH11 strain was inoculated into the seed tank, cultivated to the logarithmic growth phase, and the seed liquid was connected to the production tank for cultivation. The medium used in the production tank was the same as that of the seed tank. The liquid fermentation adopts aerobic submerged fermentation and fed-batch process, the dissolved oxygen is 15%-20%, the fermentation temperature is 30°C, the fermentation time is 72h, and the initial pH value is 7.5. After the fermentation is completed, the culture solution is taken out of the tank and directly packed into liquid dosage forms with plastic packaging barrels or packaging bottles, or subpackaged into solid dosage forms with peat adsorption packaging bags. The biocontrol strain OH11 can effectively control plant pathogenic fungi, bacteria, nematodes and other diseases, and the overall control effect is 50%-70%. In the greenhouse pot experiment, the control effects of OH11 on pepper blight and tomato bacterial wilt reached 83.6% and 86.4%, respectively. Strain OH11 has the characteristics of broad antibacterial spectrum, high activity, and environmental safety. In today's serious pesticide pollution, zymolysobacterium and its bacterial agent will be a good substitute.

An elastomeric article having reducing microbe affinity and transmission and methods for applying and immobilizing antimicrobial compounds to the elastomeric substrate surface are disclosed. The elastomeric article has a body formed of a natural or synthetic polymer latex having an outer surface and an inner surface. The body has a coating of an antimicrobial agent over at least a portion of said outer surface. The treatment involves applying according to either a spraying or dipping process an antimicrobial polymer or composition to a surface of the elastomeric substrate; binding the antimicrobial composition to the surface in a manner such that said treat antimicrobial coating passes either one or another or both versions of a zone of inhibition test, such test including: a) a dry-leaching or agar-plate-based contact test, according to AATCC 147 protocol, or b) a wet-leaching or dynamic shake flask test according to ASTM E-2149-01 protocol. The substrate is further subject to a rapid germicidal contact-transfer test of relatively short duration. The antimicrobial polymer can include an organosilane quaternary ammonium or a biguanide compound which can disrupt the ionic charges of microbial cellular membranes.

System and methods to perform inhibition diagnostic testing utilizing a Petri dish based packaged applied materials kit to conduct growth inhibition testing of essential oil compositions, herbs, antibiotics, antifungals, anti-parasititic compounds applied to fungi, bacteria or other biological materials having confluent growth patterns that are derived from a patient's inoculum grown in a personal incubator. Resulting inhibition patterns are generated by at least one essential oil and / or a panel of essential oils or other applied materials distributed in fixed concentrations or concentration gradients to confluent lawns of bacterial and / or fungal lawns derived from the patient's clinical specimen. Images are acquired during the incubation periods to measure inhibition areas or zones that developed around applied the applied material compositions and the optimal therapeutic treatment is ascertained from analysis of the developing or generated inhibition zones.

The invention belongs to the field of inorganic material, in particular relates to a preparation method of cupric oxide nanoparticle which adopts a microwave assisted method to first prepare a precursor, then carries out roasting at appropriate temperature, obtains cupric oxide nanoparticle with the structure of plate shape and tests the antibiosis performances of an inhibition zone for the powder of the cupric oxide nanoparticle. That the cupric oxide nanoparticle with the structure of special plate shape has good antibiosis effect on staphylococcus aureus and hay bacillus is discovered. The preparation method of the invention has the advantages of cleanness, high efficiency, low energy consumption, little pollution and being capable of synthesizing nano materials with high purity and uniform distribution in limited time, etc., thus starting a new field for the synthesis of nano materials.

The invention discloses a strong emulsibility microbe wax cleaning and preventing bacterial agent and an application, which belongs to the oil field chemical technology field. The wax cleaning and preventing bacterial agent mainly comprises pseudomonas aeruginosa and geobacillus sp, has strong emulsification capability, and is capable of dispersing crude oil, promoting crude oil to flow, changing adherence force of borehole wall, preventing the accumulation of wax crystal on the borehole wall, and playing the paraffin inhibition and wax cleaning effects. By increasing the initial application amount of the bacteria liquid and prolong the well closing time, a stable paraffin inhibition zone can be formed due to adhesion of bacteria on borehole wall, near wellbore formation can be cleaned, and the paraffin inhibition and oil increase effects can be increased. After on-site enforcement, the well cleaning and chemical paraffin inhibition works can not be carried out in recent half year in the test wells, so that the wax cleaning and preventing bacterial agent has good paraffin inhibition effect in the well. The oil production is increased by about 30% daily, pump efficiency is increased about 10%, the surface tension of the output liquid is decreased to 20-25%, and the crude oil condensation point is decreased by 1-3 DEG C.

The invention discloses Metschnikowia pulcherrima DPP2 with the collection number of CGMCC No. 8178. The Metschnikowia pulcherrima DPP2 has an inhibiting effect on peach postharvest monilinia fructicola, apple postharvest botrytis cinerea, apple postharvest penicillium notatum and pear postharvest penicillium notatum, as well as the inhibiting effect on peach postharvest brown rot, apple postharvest gray mold, pear postharvest blue mold and apple postharvest blue mold. In the selected plant pathogenic fungi, the inhibiting effect on the peach monilinia fructicola and the apple botrytis cinerea is most obvious, the diameters of inhibition zones are more than 20mm respectively, and the Metschnikowia pulcherrima DPP2 has the advantages of stability, high efficiency and broad-spectrum antibacterial property, and can be applied to control of pathogenic bacteria or diseases.

The invention discloses a cow leather mat with an anti-bacterial function. The cow leather mat is characterized in that a grain side and a flesh side of the cow leather mat simultaneously comprises a nano-silver and isothiazolinone antibacterial agent; the inhibition zone of the nano-silver and isothiazolinone antibacterial agent to staphylococcus aureus, colon bacillus, bacillus subtilis, Aspergillus niger, blue mould, hair mould, cryptococcus neoformans, candida albicans is 43.28 to 68.84mm; the dry wipe resistance level is 4 to 5 level or 5 level; the wet wipe resistance level is 3 to 4 level or 4 level; and folding resistance fastness is 1 level. The invention also discloses a preparation method of the cow leather mat. The cow leather mat not only has good inhibit function to bacterium and fungus, but also has durable antibacterial property; the preparation method is skillful, the only thing to do is to add antibacterial treatment after a middle layer is coated, additional investment cannot be increased, the process is simple to operate, and the cow leather mat is applicable to industrial application.

The invention discloses a ferulic acid-collagen antimicrobial film, and a preparation method and application thereof. The preparation method comprises the following steps: adding collagen in water, dissolving by stirring at the heat treatment temperature of 60-70 DEG C, sequentially adding glycerol, dialdehyde starch, sodium alginate and ferulic acid, carrying out heat treatment at 60-70 DEG C for 20-30 minutes, removing bubbles in a stirring debubbling machine, running on an organic glass plate, drying after the film forming solution is cooled, cooling at normal temperature, and peeling off the film to obtain the ferulic acid-collagen antimicrobial film. The edible food packaging film has the advantages of high transparency, high barrier property, higher mechanical strength and mold resistance; and the oxford cup method detects that the diameters of inhibition zones of the obtained film for Gram-negative bacteria and Gram-positive bacteria are 17-22mm, and the film has strong antibacterial capacity. The prepared film is used for sausage preservation, and can prolong the shelf life of sausage by 8.5 days.

The invention relates to a fast and efficient screening method of fusarium antagonistic bacteria. The screening method adopts a flat plate-based two-step culture technology and comprises the following steps of separating bacteria in a nutrient medium, spraying a fusarium spore liquid on a flat plate with the bacteria, carrying out culture at a temperature of 28 DEG C, and observing a bacterium inhibition zone around the bacteria, wherein if the bacterium inhibition zone exists around the bacteria, it is proved that the bacteria have a fusarium antagonism capacity. Through the one-flat plate two-step culture technology, nutrient medium-based purification of the bacteria separated from a sample is avoided before culture determination in another medium so that the mediums needed in the experiment are greatly reduced, labor, material resources and a cost are reduced and antagonistic bacterium screening efficiency is improved.

The invention discloses a preparation method of a nano-silver hybrid sericin porous gel antibacterial material, a product and application thereof. The preparation method includes: extracting sericin from silk, and acquiring sericin powder by freeze drying; re-dissolving the sericin powder, then performing refrigeration to prepare sericin hydrogel, and conducting freeze drying to obtain loose and porous sericin gel, then immersing the sericin porous gel in an AgNO3 solution, conducting in situ synthesis of nano-silver on the surface of the sericin porous gel by means of ultraviolet radiation reduction, thus obtaining the nano-silver hybrid sericin porous gel antibacterial material. Growth curve determination and inhibition zone analysis show that the nano-silver hybrid sericin porous gel antibacterial material prepared by the method provided by the invention has a significant inhibitory effect on growth of escherichia coli and staphylococcus aureus. The method provided by the invention has the characteristics of simple operation, mild condition, green and environmental protection, and is expected to prepare antibacterial materials for the field of biomedical materials.

The invention provides a Bifidobacterium longum LJM002 strain, of which the collection number is CGMCC No.4900. The LJM002 strain is super-sensitive to acephate, and thus, is a working strain for detecting acephate residues in food. The invention also provides a microbiological method for detecting acephate residues in food, which determines whether the residue level of acephate in a food sample to be detected is overproof according to the size of the inhibition zone on the detection culture medium. The method provided by the invention is simple to operate, has the characteristics of low cost and high sensitivity, is suitable for sample detection, and satisfies the detection requirements of Chinese Pesticide Residue Detection Department for mass samples.

The invention relates to application of eugenol and in particular relates to application of eugenol in food-borne pathogenic bacterium in-vitro inhibition, culture mediums of food-borne pathogenic bacterium are TSA culture mediums or TSB culture mediums with pH values being 5.0, 6.0 and 7.0; the diameter of an inhibition zone is measured for judging antibacterial activity of eugenol; and a multiple dilution method is adopted for carrying out minimal inhibitory concentration (MIC) determination on various bacteria. The application of eugenol in the food-borne pathogenic bacterium in vitro inhibition has the advantages that escherichia coli, staphylococcus aureus, salmonella and shigella are taken as research objects, MIC of 2-methoxy-4-(2-propenyl) phenol on common pathogens in food is researched, influence of pH on antibacterial effect of 2-methoxyl-4-(2-propenyl) phenol is studied, and application value of 2-methoxy-4-(2-propenyl) phenol in food antisepsis aspect is researched; and application range of 2-methoxy-4-(2-propenyl) phenol can be beneficially expanded.

The invention relates to an extraction method and an antibacterial activity screening method for fat-soluble active ingredients in insects and arthropods. The extraction method comprises the following steps: digesting dried insects or arthropods twice with methylene chloride at the room temperature; performing vacuum filtration on a leaching solution, combining filtrates, and recovering a solvent under reduced pressure to obtain insect methylene chloride crude extract; placing the obtained crude extract into an evaporation pan, heating in a water bath at the temperature of 40-50 DEG C for 6-8 hours, mixing the crude extract with distilled water in the volume ratio of 1:(2-4), extracting with petroleum ether, standing for layering, and recovering a water phase; concentrating the water phase, transferring into the evaporation pan, and drying in the water bath at the temperature of 40-50 DEG C to obtain fat-soluble active ingredients from which insect wax is removed in insects and arthropods. In antibacterial activity screening, Gram-positive and Gram-negative bacterial strains are taken as research objects, an antibiotic is taken as a positive control, 70 percent methanol is taken as a blank control, and the antibacterial activity is balanced and compared via the diameter of an inhibition zone measured with a flat band method.

The invention provides a method for preparing nano-silver composite particles from emblica polysaccharide and belongs to the technical field of nanometer materials. The method comprises the steps of preparation of emblica polysaccharide and preparation of nano-silver. According to the prepared composite particles, the diameter, measured through bacteria inhibition experiments, of inhibition zones is 27.9+ / -0.01 mm; a theoretical value and an experimental value are basically equal. This shows that the process for preparing AgNPs through a response surface optimization method is green and reliable.

The invention belongs to the technical field of culture of lactic acid bacteria, and relates to lactobacillus plantarum for feeding, and a culture method and application of the lactobacillus plantarum. The lactobacillus plantarum WEI-70 with a preservation number of China General Microbiological Culture Collection Center (CGMCC) No. 11747 is separated from feces of healthy piglets which are 60 days old, can tolerate pig bile salts with low pH values and high concentrations, and has a higher inhibition effect for escherichia coli, salmonella, streptococcus and staphylococcus aureus; the inhibition zone of the lactobacillus plantarum WEI-70 can reach 30-35mm. The high density liquid culture method of the lactobacillus plantarum WEI-70 with the preservation number of CGMCC No. 11747 is obtained by optimizing the formula, culture temperature and optimal fermentation initial pH value of a culture medium; the maximum viable number of fermentation liquor can reach 6.0*10<9> CFU / mL and is not less than six times the viable number obtained by using an MRS culture medium under the same culture conditions. The thalli of the fermentation liquor are collected by using a high speed tubular centrifuge, are then mixed with a special protective agent according to a certain ratio and are subjected to vacuum freezing-drying; the maximum viable number of the obtained freeze-dried bacteria powder can reach 7.0*10<11> CFU / g, and the survival rate of the freeze-dried bacteria powder can reach 93%.

The present invention relates to sterilization, and is especially Atexadrium tamarense culture liquid sterilizing process. The effective reliable Atexadrium tamarense culture liquid sterilizing process includes the following steps: plate separating bacteria from the Atexadrium tamarense culture liquid, inoculating to culture medium and painting to plate; sticking antibiotic containing medicine sensitive paper to the surface of the plate, turning the plate for culturing to reaching stable inhibition zone, filtering culture liquid in exponential phase and suspending Atexadrium tamarense cell in the filter membrane in f / 2 culture liquid; centrifuging to eliminate supernatant, re-suspending in f / 2 culture liquid and setting under illumination; adding lysozyme, adding SDS, setting under illumination, eliminating lysozyme and SDS, re-suspending in f / 2 culture liquid and setting under illumination; at least thrice passage of the treating culture liquid, eliminating antibiotic and inspection.

The invention relates to a slow-release and anti-infective composite soft tissue repair material and a preparation method thereof. The repair material is prepared from a biological material and media containing anti-infective components, wherein the media containing the anti-infective components are arranged between layers of the biological material or on the surface of the biological material at intervals according to the radius of an inhibition zone of a drug sensitive test, and a whole body is formed by the aid of a binding agent, suture lines or pressure. According to the slow-release and anti-infective composite soft tissue repair material and the preparation method thereof, the anti-infective components can be released effectively and continuously, meanwhile, biocompatibility of the biological material is not changed, the biological material is non-toxic and harmless, and the application prospect is good.

The invention provides a pharmaceutical composition for controlling bacterial infectious diseades of livestock and poultry. The pharmaceutical composition is characterized in that 14 Chinese herbal medicines (include persicaria perfoliata, polygonum cuspidatum, dandelion, acalypha australis, pulsatilla chinensis, glossy privet fruit, phyllanthus urinaria, sanguisorba officinalis, radix bupleuri, trifoliate orange leaf, sculellaria barbata, euphorbia humifusa, cyrtomium fortune and hawthorn) are selected from the general 60 Chinese herbal medicines by screening through a flat paper piece bacterial inhibiting method, wherein the 14 Chinese herbal medicines have a large inhibition zone and high effect; and the 14 Chinese herbal medicines are dried, crushed and uniformly mixed to obtain the pharmaceutical composition. The experimental study shows that the bacteriostatic test effect of the obtained pharmaceutical composition is obviously improved after adding trimethoprim (TMP) and sulfamonomethoxine to the Chinese herbal medicines. The pharmaceutical composition having good effect on controlling the bacterial infectious diseades of livestock and poultry can be directly added to the feed of livestock and poultry, and brings satisfaction effect in clinic.

The invention relates to a long-acting mildew-proof polyacrylate modified casein composite emulsion and a preparation method thereof. If being used as a mildew preventive, a casein emulsion easily mildews after forming a film. The preparation method comprises the steps of placing hollow ZnO microspheres into a 2-thiocyanomethylthio)benzothiazole solution, aging and centrifugally drying to obtain medicine-carrying hollow ZnO, placing the medicine-carrying hollow ZnO into a dopamine solution, oscillating in a water bath, and centrifugally drying to obtain a dopamine-coated modified medicine-carrying hollow ZnO composite mildewcide; and dropwise adding triethanolamine into a casein water solution, filtering, adding water, heating, adding a caprolactam solution in twice, dropwise adding a butyl acrylate and methyl methacrylate mixed solution and an ammonium persulfate solution to obtain a polyacrylate modified casein emulsion, adding a dopamine-coated modified medicine-carrying hollow ZnO composite mildewcide solution into the emulsion, and carrying out ultrasonic treatment to obtain the long-acting mildew-proof polyacrylate modified casein composite emulsion. A long-acting mildew-proof polyacrylate modified casein composite film prepared from the long-acting mildew-proof polyacrylate modified casein composite emulsion has the aspergillus flavus inhibition zone of 19mm (the diameter of the composite film is 15mm) and has a relatively good mildew-proof effect.

The invention discloses a jute root extract with antibacterial activity and a preparation method thereof. The method relates to a comprehensive jute utilization technology, and is used for preparing extracts. The jute root extract serves as a natural antibacterial preservative and an antibacterial finishing agent, and belongs to the field of application of foods, medicaments and textiles. The method comprises the following steps of: washing jute root serving as a raw material with water; drying; grinding; extracting ethanol under ultrasonic assistance; concentrating under the reduced pressure; and adsorbing and purifying with a macroporous resin to obtain the jute root extract with high antibacterial activity. The antibacterial effect of the jute root extract is detected by using gram-positive bacterium staphylococcus aureus, bacillus subtilis, candida albicans and gram-negative bacteria escherichia coli as tested bacteria by an in-vitro bacteria inhibition zone method. By adopting the method, the jute root extract with antibacterial activity has best antibacterial effect on the staphylococcus aureus and bacillus subtilis, better antibacterial effect on candida albicans and good antibacterial effect on escherichia coli.

The invention discloses Bacillus amyloliquefaciens F9 and application thereof in prevention and control of citrus bacterial canker. The strain F9 is preserved in China Center for Type Culture Collection in Wuhan University, China on September 26th, 2016 with the preservation number of CCTCC NO:M 2016519. Plate antagonistic experiments find that the strain F9 has citrus bacterial canker inhibitory activity, and an inhibition zone is 26.5mm; supernatant of fermentation broth of the strain F9 after 4 days has antibacterial activity, and the diameter of the inhibition zone can reach 26.5mm; and an in vitro inoculation experiment finds that the strain F9 can inhibit extension and colonization of xanthomonas citri. Therefore, the Bacillus amyloliquefaciens F9 can be applied to prevention and control of the citrus bacterial canker as a biocontrol bacterium.

The invention provides ribosome resistance mutagenesis breeding vancomycin production bacterial strain and an application thereof, specifically the mutagenesis breeding method of mutagenesis bacterial strain of Amycolatopsis orientalis and the application thereof in preparing vancomycin, relating to breeding of microorganism. The method comprises the following steps: inoculating the Amycolatopsis orientalis to Gause-1 agar culture medium panel to be cultured; taking out spore and using sterile water to prepare spore suspension and coating the spore suspension in Gause-1 agar culture medium panel featuring mixing resistance of vancomycin and streptomycin to be cultured; randomly selecting produced single bacterial colony and streak-inoculating the single bacterial colony to the Gause-1 agar culture medium panel to be cultured, carrying out biological activity determination on produced mutagenesis bacterial strain; selecting mutagenesis bacterial strain with inhibition zone diameter thereof being 2mm larger than the inhibition zone diameter of original strain and inoculating the mutagenesis bacterial strain to liquid fermentation medium to be cultured; determining the content of vancomycin in fermentation liquor by HPLC method to verify the obtained vancomycin mutagenesis bacterial strain; subjecting the obtained vancomycin mutagenesis bacterial strain to 5 times of continuous passage stability inspection to obtain the vancomycin production bacterial strain.

The invention discloses a bacterium inhibition level identification method based on a plate experiment and deep learning. The method comprises the following steps of: S1, determining the position of amedicine sensitive paper sheet; S2, measuring the size of an inhibition zone; S3, constructing a sample set: constructing a sample set having a label through using a deep learning model, wherein thesample set can be used for training a model; S4, constructing and training a model: constructing a model that can be used for identifying the medicine sensitive paper sheet, and training the model byusing the sample set; S5, adopting the model to identify the medicine sensitive paper sheet; and S6, judging the bacterium inhibition level of a medicine: identifying the type of the medicine by usingmodel, inquiring a corresponding bacterium inhibition standard from a database, determining an interval to which the diameter of the inhibition zone belongs in the standard, and obtaining the bacterium inhibition level of the medicine. With the method of the invention adopted, an operator only needs to photograph a plate image so that the operator can identify the medicine type to which the medicine sensitive paper sheet belongs and the size of the inhibition zone corresponding to the medicine type from the image, and therefore, the automatic identification of bacterium inhibition levels canbe possible.

The present invention relates to process and apparatus for screening antibiotic producing strain. The apparatus has one upper layer of culture dishes and one lower layer of culture dishes separated with one semi-permeable microporous film. After abacterial liquid culture medium connecting agar in 10-15 g / l for the bacterial strain to be screened is fed to the culture dishes in one layer and solidified and the bacterial strain to be screened is inoculated and cultured for sufficient time, indicator bacterium is inoculated to the culture dishes in the other layer, the bacterial strain to be screened and the indicator bacterium are cultured simultaneously and the indicator bacterium culturing medium is observed for whether to have inhibition zone. The process of the present invention is simple, fast, high in screening efficiency and low in cost.

The invention provides a blended fabric comprising bamboo-hemp-cotton fibers, comprising bamboo fibers, hemp fibers and cotton fibers in a mass ratio of (30-50): (15-30): (20-50). The blended fabric comprising bamboo fibers, hemp fibers and cotton fibers can be used for sewing bedding; the blended fabric has good smoothness, softness and affinity and excellent moisture absorption and moisture permeability, so that the bedding sewn from the blended fabric can improve the comfort of human body; and the blended fabric has an effective antibacterial effect, and can balance the metabolism of skin, adjust human microcirculation and improve the sleep quality. When being used for antibacterial activity tests, the blended fabric has an inhibition zone, which indicates that the blended fabric has good antibacterial effect; and after the experience of 100 sleep test persons, the skin glossiness of 80% of individuals is improved, and the sleep quality of 85% of individuals is obviously improved.

The invention relates to a drug sensitivity paper inhibition zone size measuring method based on images; the method uses a mobile phone or a digital camera to shoot a dish, more accurately identifiesthe inhibition zones in the image, and calculates the sizes of the inhibition zones; the process comprises the following steps: preprocessing the image, determining dish and drug sensitivity paper positions, finding candidate circles for the inhibition zones, screening the optical circle of the inhibition zones, and calculating the sizes of the inhibition zones. The method can greatly improve theinhibition zone measuring efficiency with a high measuring precision, and no large special equipment is needed, thus reducing work burdens of research personnel; the method can be promoted to basic units with incomplete equipment, thus providing conveniences for the work researches.

The invention relates to the field of microbe screening and specifically discloses a method for rapid screening of Bacillus spp with antibacterial activity. According to the invention, high temperature treatment is carried out to rapidly eliminate competitors, agar overlaying is realized via an indication bacterium, and Bacillus spp with antibacterial activity is screened by observing an inhibition zone; and under a culture condition of 35 to 42 DEG C, the inhibition zone can be observed within 6 to 12 h of culture after agar overlaying, so high-efficiency screening of Bacillus spp with antibacterial activity is realized, the whole screening process can be completed within 48 h, screening procedures are substantially simplified, and labor intensity is mitigated. The method only utilizes a plurality of simple medium and does not need test materials like filter paper and an Oxford cup, so screening cost is low.

The invention discloses a compound bacterium agent for preventing and treating bacterial diseases of tobacco. The bacterium agent is prepared through mixing biological control bacteria A and biological control bacteria B according to the volume ratio of (1 to 2): (1 to 2), wherein the preservation number of the biological control bacteria A is CGMCC No. 10603, and the preservation number of the biological control bacteria B is CGMCC No. 11541. According to the compound bacterium agent disclosed by the invention, the biological control bacteria A and the biological control bacteria B are symbiotic and can simultaneously and stably exist in the compound bacterium agent, so that a technical support is provided for biological prevention and control on tobacco diseases, meanwhile, the consumption of chemical pesticides can be effectively lowered, and thus the compound bacterium agent plays a role in protecting environment. By using the compound bacterium agent provided by the invention, the growth of tobacco wildfire and angular leaf spot bacteria can be effectively inhibited, the inhibition zone of wildfire diseases can reach 25mm, and the inhibition zone of angular leaf spot can reach 28mm.