11 results about "Fire blight" patented technology

The present disclosure relates to a phosphor compound for diagnosing fire blight in fruit trees based on fluorescence and use thereof. The compound is capable of selectively detecting Erwinia amylovora, the causal pathogen of fire blight in fruit trees, and emits red fluorescence in response. Due to its rapid diagnostic response and high sensitivity, the compound can be effectively used for diagnosing fire blight in fruit trees through various simple methods such as spraying or swabbing, without the need for specialized equipment.

The application discloses a microbial source bactericidal composition containing Leuconostoc mesenteroides WZ-44 and kasugamycin and application thereof. The bactericidal composition takes Leuconostoc mesenteroides WZ-44 fermentation liquor (or fermentation sterile filtrate) and kasugamycin as main effective components, wherein the mass ratio of the Leuconostoc mesenteroides WZ-44 fermentation liquor (or fermentation sterile filtrate) to the kasugamycin is 4999:1-99:1, and is further preferably 1499:1-499:1. The bactericidal composition shows a synergistic effect within the above-mentioned ratio range, overcomes and delays drug resistance of pathogenic bacteria, has a good prevention and control effect on pear fire blight, a long effective period and low application cost, is obviously superior to a single agent in the prevention and control effect, and greatly reduces the use amount of the kasugamycin.

The invention discloses application of p-allylphenol in preparation of an inhibitor for preventing and treating pear fire blight and preparation of p-allylphenol, and relates to the technical field of biology. The preparation of p-allyl phenol comprises the following steps: obtaining spores of Streptomyces sp.D67, inoculating the spores to a culture medium for cultivation, and carrying out purification treatment on the obtained crude extract to obtain p-allyl phenol. The effective concentration of p-allylphenol is 0.125 mg / mL-0. 5mg / mL, and it is found that p-allylphenol has quorum sensing inhibition activity on pear fire disease pathogenic bacteria. The p-allylphenol is applied to the preparation of the inhibitor for preventing and treating pear fire blight, the inhibition rate of the p-allylphenol on the generation of a pear fire blight pathogenic bacteria biofilm reaches 69.3%, and the inhibition rate of the p-allylphenol on the formation of exopolysaccharides reaches 39.9%; the p-allylphenol has an outstanding technical effect on solving the pear fire blight, and provides a new choice for biological prevention and control application of the pear fire blight.

The invention discloses a method for inhibiting growth of pathogenic bacteria of pyretic blight and delaying pyretic blight infection of bergamot pears by using exogenous spermine. In the research process, a bacterial solution of Erwinia amylovora is streaked and inoculated to NA culture media of exogenous polyamines with different concentrations for culture, and the growth inhibition condition of bacterial colonies is observed. The method comprises the following steps: selecting young fruits of Korla bergamot pears, sterilizing, needling and inoculating pathogenic bacteria of fire blight, then putting the young fruits into an inoculation plate paved with wet gauze, culturing, then respectively spraying equal amount of distilled water (contrast) and exogenous polyamines of different types and concentrations, continuously culturing for 2-3 days, and observing the scab formation and expansion conditions of the young fruits. Research results show that by applying the exogenous spermine with the concentration of 1.0 mmol / L, in-vitro growth of pathogenic bacteria of the fire blight can be remarkably inhibited, and the infection process of the pathogenic bacteria of the fire blight to young bergamot pear fruits can be effectively delayed. The invention provides a new technical means for green prevention and control of the fire blight of the Korla pear.

The invention discloses a quorum sensing inhibitor and application of the quorum sensing inhibitor in prevention and treatment of pear fire disease pathogenic bacteria, the effective component of the quorum sensing inhibitor is 9 amino-1, 2, 3, 4,-tetrahydroacridine, by applying the quorum sensing inhibitor provided by the invention, the inhibition rate of biofilm generation of the pear fire disease pathogenic bacteria can reach 93.4%, and the inhibition rate of exopolysaccharide formation can reach 66.4%; the quorum sensing inhibitor provided by the invention has obvious inhibition activity on quorum sensing of pear fire disease pathogenic bacteria, and the activity of extracellular enzymes related to pathogenic bacteria infection, including protease, cellulase, xylanase, pectate lyase and polygalacturonase, is obviously reduced, so that the quorum sensing inhibitor provided by the invention has obvious inhibition activity on quorum sensing of the pear fire disease pathogenic bacteria, and can be used for preparing the quorum sensing inhibitor for pear fire disease pathogenic bacteria by reducing expression of virulence factors of the quorum sensing inhibitor. Therefore, the infection process is delayed, the infectivity of pear fire blight is reduced, and the damage to plants is reduced. According to the technical scheme provided by the invention, an innovative path from'pathogenic mechanism analysis' to'ecological adaptability intervention 'is opened up for comprehensive prevention and control of pear fire blight.

The present invention relates to new bacteriophages with SEQ ID NO.: 1-2 against Erwinia amylovora infections and use of same for treating and / or preventing an Erwinia amylovora infection in a crop.

The present application relates to a Paenibacillus polymyxa strain Y31, a microbial inoculum prepared therefrom and applications thereof. The strain Y31 can effectively inhibit the growth of Dothiorella gregaria, and field control tests show that the comprehensive prevention and control effect of the microbial inoculum prepared from the strain Y31 on the disease is as high as 72% or more in the early stage of the disease. Antagonistic experiments show that the strain Y31 also has good bacteriostatic effect on Dothiorella septospora and Dothiorella needle red rot fungus, and is suitable for being used as a broad-spectrum antibacterial agent in the biocontrol practice of pine fungal diseases. Meanwhile, the strain Y31 also has obvious inhibitory effect on bacterial diseases such as willow bacterial wilt fungus, pear fire blight fungus, walnut bacterial black spot fungus and kiwi fruit canker fungus, and can be used for the prevention and control of diseases such as willow wilt. In addition, the strain Y31 also has functions such as nitrogen fixation, phosphorus and potassium solubilization, and has certain salt and alkali tolerance, and can be used for promoting growth and disease resistance of plants in saline-alkali land.

This invention relates to the field of biological control, and in particular to a compound agent for controlling pear fire blight, its preparation method, and its application. This invention provides a compound agent with Bacillus vesicularis FX1 fermentation broth and kasugamycin aqueous solution as active ingredients, which can effectively control pear fire blight. The compound agent, by combining Bacillus vesicularis FX1 fermentation broth and kasugamycin aqueous solution, exhibits a synergistic effect. It leverages the high efficiency and rapid bactericidal advantage of kasugamycin while increasing the colonization of Bacillus vesicularis FX1 on the tree surface, making it the dominant foliar flora and constructing a stable and balanced micro-ecosystem. This can reduce the use of chemical pesticides by 60%, leveraging the synergistic and complementary effects of bacteria and pesticides to improve disease control, achieving the goal of safe, efficient, and sustainable control of the occurrence, spread, and diffusion of the disease.

This application discloses a strain of Bacillus belyceae DW4-5-2 for controlling pear fire blight and develops a highly efficient and stable microbial preparation fermentation method for it, clarifying its application in the control of pear fire blight. Compared with existing technologies, this application has the following advantages: the strain is highly novel; it has high antagonistic activity; it has excellent pot-plant control efficacy and a long-lasting effect; the fermentation process is thoroughly optimized; and it has broad application prospects. This strain and its optimized fermentation process provide core technical support for the development of highly efficient, stable, and environmentally friendly biological pesticides for pear fire blight, which is of great significance for ensuring the green, safe, and sustainable development of my country's pear and apple industries.

This utility model discloses a pear blight monitoring toolbox, including a base plate, a lower compartment fixedly connected to the top surface of the base plate, a middle compartment slidably disposed on the top surface of the lower compartment, and an upper compartment slidably disposed on the top surface of the middle compartment. The top surfaces of the lower, middle, and upper compartments are all open structures. Each of the lower, middle, and upper compartments contains a tool compartment, and multiple partitions are fixedly connected inside the tool compartment. Two first top sliding grooves are formed on both sides of the top surface of the lower compartment, and two first top sliding blocks are fixedly connected to both sides of the bottom surface of the middle compartment. The first top sliding blocks are horizontally slidably connected within the first top sliding grooves. Two second top sliding grooves are formed on both sides of the top surface of the middle compartment. The lower, middle, and upper compartments are slidably disposed, allowing them to be opened horizontally to display all tools for easy access and use, making it more practical.

The present disclosure relates to a phosphor compound for diagnosing fire blight in fruit trees based on fluorescence and use thereof. The compound is capable of selectively detecting Erwinia amylovora, the causal pathogen of fire blight in fruit trees, and emits red fluorescence in response. Due to its rapid diagnostic response and high sensitivity, the compound can be effectively used for diagnosing fire blight in fruit trees through various simple methods such as spraying or swabbing, without the need for specialized equipment.