37 results about "Fire blight" patented technology

The present disclosure relates to a phosphor compound for diagnosing fire blight in fruit trees based on fluorescence and use thereof. The compound is capable of selectively detecting Erwinia amylovora, the causal pathogen of fire blight in fruit trees, and emits red fluorescence in response. Due to its rapid diagnostic response and high sensitivity, the compound can be effectively used for diagnosing fire blight in fruit trees through various simple methods such as spraying or swabbing, without the need for specialized equipment.

The invention relates to the technical field of plant disease-resistant gene identification and genetic engineering, in particular to a pyrus betulaefolia lectin receptor-like kinase gene sequence and application of the pyrus betulaefolia lectin receptor-like kinase gene sequence to improvement of apple and pear rot disease resistance. The pyrus betulaefolia canker-resistant gene sequence provided by the invention is used for improving the ability of resisting saprophytic fungus canker. The pyrus betulaefolia anti-disease gene provided by the invention is obtained by cloning from a pyrus betulaefolia L-LecRKs family for the first time, the function and possible action mechanism of the anti-disease gene PbeL-LecRK-S.4 in the aspect of disease resistance are further studied, and a theoretical basis is provided for breeding of pyrus betulaefolia for disease resistance. According to the invention, a pFGC5941-PbeL-LecRK-S.4 overexpression vector and a PTRV2-PbeL-LecRK-S.4 silence vector are constructed for the first time, and the pFGC5941-PbeL-LecRK-S.4 overexpression vector and the PTRV2-PbeL-LecRK-S.4 silence vector are introduced into pyrus betulaefolia ('Duli-G03') cells through transient expression verification of Huangguan pear fruits and agrobacterium tumefaciens-mediated transformation. The disease-resistant response of a pyrus betulaefolia disease-resistant gene PbeL-LecRK-S.4 overexpression suspension cell line and wild pyrus betulaefolia suspension cells after inoculation of the rot disease is studied. The pyrus betulaefolia anti-disease gene PbeL-LecRK-S.4 provided by the invention can obviously improve the resistance of pyrus betulaefolia to valsa ceratosperma.

The application discloses a method for preventing and treating trunk pear fire blight spots of fragrant pear, which comprises the following steps: a medicament mixed solution is prepared by mixing bacillus amyloliquefaciens diluted 300-400 times with water and manganese zinc oxide diluted 400-500 times with water in a weight ratio of 1-1.5:1-1.5; when the trunk of the fragrant pear is diseased in June every year, diseased tree bark at a trunk pus flow is cut off by using an electric saw without damaging the trunk wood; the prepared medicament mixed solution is evenly applied to the disease spots by using a brush, and the second application is performed after one week. The method is simple, fast, low in cost and high in operability, the prevention effect can reach 100% when the trunk disease spots have not caused the trunk to appear the dead symptoms, and the prevention effect can reach about 75% when the trunk has appeared the dead symptoms, the trunk can gradually recover, new buds can be generated, and new branches can be formed.

The present invention discloses a method for controlling pear tree pests and diseases using a combination of predatory mites and Bacillus amyloliquefaciens. The predatory mite is Neoseiulus barkeri. The method comprises the following steps: 1) preparing biocontrol bacteria: preparing a Bacillus amyloliquefaciens powder; 2) preparing predatory mites: preparing adult Neoseiulus barkeri mites in a matrix material; 3) preparing a mixed biocontrol product: mixing the Bacillus amyloliquefaciens powder with the matrix material containing Neoseiulus barkeri mites before use; the mixed biocontrol product is sealed in a package and stored at 13-17°C; 4) applying the agent: applying the mixed biocontrol product during the second physiological fruit drop period of the pear tree, opening the package and securing it to the trunk of the pear tree; the mixed biocontrol product used per pear tree contains 160-190 million CFU of Bacillus amyloliquefaciens and 2,900-3,100 Neoseiulus barkeri mites. The method can simultaneously control pear mite pests and pear fire blight, achieving a fire blight control efficacy of over 80%.

The invention relates to the technical field of agricultural biology, and particularly provides a method for detecting pear fire blight viable bacteria based on PMAxx-qPCR and application. By optimizing the treatment concentration and exposure time of PMAxx and combining a qPCR method, the pear fire blight viable bacteria are quantitatively detected. The method can effectively eliminate dead bacteria interference in a specific range, and is suitable for accurate quantification of the viable count in a bacterial liquid concentration range of 10 < 3 >-10 < 8 > cfu / mL. The detection method has the advantages of high specificity, high accuracy, high universality, high sensitivity and the like.

The subject of the invention is new strain Pantoea allii T14 / 15 strain deposited in the Polish Collection of Microorganisms PCM at the Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences named after Lud-wik Hirszfeld in Wroc aw. The subject of the invention is also the use of the new strain Pantoea allii T14 / 15 as agent for protecting vegetable, fruit, agricultural, and ornamental plants against fungal diseases such as white rot, gray mold, alternariosis, anthracnose, brown rot, bull's eye rot, powdery mildew and blue mold, as well as bacterial diseases such as fire blight, soft rot, affecting cultivated plants, both during the growing season and during the storage of crops. Strain Pantoea allii T14 / 15 is applied in the form of an aqueous suspension to the plant's surface, through spraying, soaking plants or their parts under storage conditions, under cover, and in field conditions, and the cell count of the strain in the suspension is at least 107 CFU / ml.

The invention discloses a rapid and efficient pear and apple canker resistance evaluation method, and relates to the technical field of horticultural plant disease resistance evaluation.The method comprises the following steps of S1, test branch preparation, S2, pathogenic bacterium culture, S3, contact method inoculation and S4, resistance evaluation.The method can accurately evaluate the canker resistance of pear and apple resources, and the method has the advantages of being high in practicability and high in practicability. The method has the advantages of simple operation, no need of punching or preparation of fungus cakes, simplified operation steps, completion of inoculation of 100-120 parts of materials within 2 hours, efficiency more than 10 times of that of a traditional punching method, suitableness for rapid screening of large-scale germplasm resources, suitableness for canker resistance evaluation of different varieties of pears and apples, germplasm resources and filial generations, and wide application prospect. And a technical support is provided for disease-resistant variety breeding, resistance gene mining and green prevention and control.

Disclosed herein are methods and compositions for use in controlling citrus greening, citrus canker, or fire blight, wherein the compositions comprise one or more antimicrobial peptides, or one or more polynucleotides encoding for the one or more antimicrobial peptides.

Disclosed herein are methods and compositions for use in controlling citrus greening, citrus canker, or fire blight, wherein the compositions comprise one or more antimicrobial peptides, or one or more polynucleotides encoding for the one or more antimicrobial peptides.

The present invention provides the application of a pear short peptide PbePEP4 in plant disease prevention and control, specifically the application of a pear (Pyrus betulifolia) short peptide PbePEP4 in the prevention and control of apricot fire blight, rapeseed sclerotinia and watermelon gray mold. The present invention artificially synthesizes the short peptide PbePEP4 for the first time, clarifies its stimulating effect on the resistance to the above-mentioned diseases, and provides a method of spraying pears, rapeseed and watermelon to stimulate their resistance to fire blight, sclerotinia and gray mold, thereby preventing and controlling the above-mentioned diseases. The PbePEP4 short peptide provided by the present invention is a product synthesized by the plant itself. The use of the short peptide to stimulate immunity and thus prevent and control diseases has the advantages of being natural, green, environmentally friendly, and not inducing pathogens to develop drug resistance. In addition, the short peptide is easy to synthesize artificially, is easy to obtain, has a strong disease resistance stimulating effect, and requires a low concentration. It is a new type of ecological and environmentally friendly crop disease prevention and control preparation.

The present invention discloses a highly thermostable mutant of AHL-lactonase and its application in biological control of fire blight of fruit trees. The mutation site of the mutant is to mutate the amino acid sequence from position 176 to position 181 of the AHL-lactonase shown in SEQ ID NO.3 from the amino terminus to the carboxyl terminus. The mutated amino acid sequence is shown in SEQ ID NO.1, denoted as mutant A19-9, or to mutate the amino acid sequence from position 176 to position 184 from the amino terminus to the carboxyl terminus. The mutated amino acid sequence is shown in SEQ ID NO.2, denoted as mutant C13-1. The thermostability of both mutants has been greatly improved compared to the wild type. When the two mutants are applied to prepare a biological pathogenic bacteria inhibitor, the inhibitory Erwinia amylovora effect on fire blight of apple caused by infection is better than that of the wild type, and it provides a potential biological control agent for E.amylovora fire blight of fruit trees caused by infection.

The application discloses a microbial source bactericidal composition containing Leuconostoc mesenteroides WZ-44 and kasugamycin and application thereof. The bactericidal composition takes Leuconostoc mesenteroides WZ-44 fermentation liquor (or fermentation sterile filtrate) and kasugamycin as main effective components, wherein the mass ratio of the Leuconostoc mesenteroides WZ-44 fermentation liquor (or fermentation sterile filtrate) to the kasugamycin is 4999:1-99:1, and is further preferably 1499:1-499:1. The bactericidal composition shows a synergistic effect within the above-mentioned ratio range, overcomes and delays drug resistance of pathogenic bacteria, has a good prevention and control effect on pear fire blight, a long effective period and low application cost, is obviously superior to a single agent in the prevention and control effect, and greatly reduces the use amount of the kasugamycin.

The invention provides a specific nucleic acid aptamer capable of being combined with pear fire blight bacteria and application of the specific nucleic acid aptamer, and the provided nucleic acid aptamer is an ssDNA nucleic acid aptamer with high affinity and high specificity to the pear fire blight bacteria and can be used for preparing biological products for preventing and treating the pear fire blight bacteria. The sequence of the nucleic acid aptamer is as shown in SEQ ID NO: 1-3. Through flow cytometry and KD determination, the aptamer provided by the invention has ultrahigh affinity and strong specificity, and can be widely applied to detection, prevention and control and mechanism research of pear fire blight bacteria.

The invention discloses a microbial aqueous solution for preventing and treating pear fire blight and a preparation method thereof, and belongs to the technical field of microbial fermentation. The preparation method comprises the following steps: carrying out co-fermentation on a compound strain constructed by two bacillus velezensis to obtain fermentation liquor, and adding an auxiliary agent with good biocompatibility to obtain a water aqua preparation. The preparation is simple and convenient in fermentation process, short in production period, low in cost, convenient and safe to use, good in environmental stability, easy to store and excellent in disease prevention effect, and a new product is provided for safe and green prevention and control of pear fire blight.

The invention discloses application of p-allylphenol in preparation of an inhibitor for preventing and treating pear fire blight and preparation of p-allylphenol, and relates to the technical field of biology. The preparation of p-allyl phenol comprises the following steps: obtaining spores of Streptomyces sp.D67, inoculating the spores to a culture medium for cultivation, and carrying out purification treatment on the obtained crude extract to obtain p-allyl phenol. The effective concentration of p-allylphenol is 0.125 mg / mL-0. 5mg / mL, and it is found that p-allylphenol has quorum sensing inhibition activity on pear fire disease pathogenic bacteria. The p-allylphenol is applied to the preparation of the inhibitor for preventing and treating pear fire blight, the inhibition rate of the p-allylphenol on the generation of a pear fire blight pathogenic bacteria biofilm reaches 69.3%, and the inhibition rate of the p-allylphenol on the formation of exopolysaccharides reaches 39.9%; the p-allylphenol has an outstanding technical effect on solving the pear fire blight, and provides a new choice for biological prevention and control application of the pear fire blight.

The invention belongs to the technical field of prevention and treatment of bacterial diseases, and particularly relates to application of disulfide in prevention and treatment of pear fire blight. According to the present invention, the disulfide can strongly inhibit the T3SS of the pear fire blight bacteria while the growth of the pear fire blight bacteria is not affected, such that the pathogenicity of the pathogenic bacteria is significantly reduced, and the effect of treating the pear fire blight caused by the pear fire blight bacteria is achieved while the growth of the pear fire blight bacteria is not affected. Therefore, the disulfide or the pharmaceutically acceptable salt thereof can be used as a pathogenic bacterium T3SS inhibitor, can be prepared into a medicament for preventing and / or treating the pear fire blight, and also has the effect of reducing and delaying the drug resistance of the pear fire blight to the compound, so that the disulfide or the pharmaceutically acceptable salt thereof can be used for preparing the medicament for preventing and / or treating the pear fire blight. The effective service life is relatively long in the aspect of preventing and / or treating pear fire blight, and the application prospect is wide.

The invention discloses an application of a pear fire blight bacterium effect factor HrpG in prevention and control of pear fire blight. A nucleotide sequence of the effect factor HrpG gene is shown as SEQ ID NO: 1. Through a gene editing technology, the pyraclospermum palmatum mutant delta HrpG of which the effector HrpG gene is knocked out is constructed. Korla young fruits and pyrus betulaefolia in-vitro leaves are inoculated through a control group of wild fire blight bacteria and delta HrpG, and the delta HrpG is found to be slower in morbidity and smaller in scabs; the mutant delta HrpG is applied to plants or a plant growth environment, and biological prevention and control of fire blight are achieved through the competitive effect of the mutant delta HrpG and wild type pathogenic bacteria. Therefore, delta HrpG is weaker in pathogenicity, and provides a new strategy for green prevention and control of pear fire blight in application.

The invention discloses a pear fire and blight resistant pear stock tissue culture environment control method, and particularly relates to the technical field of plant tissue culture, which comprises the following steps: acquiring plant tissue metabolism and growth data, inputting the data into a pre-trained machine learning model, and outputting a result whether a culture medium needs to be replaced or not; when the culture medium needs to be replaced, the model predicts and optimizes a component parameter group of the culture medium; performing feature extraction and analysis on the optimized culture medium parameter group and the current culture medium parameter group to generate culture medium deviation and plant adaptability data; based on an analysis result, determining the duration of a buffer stage through buffer judgment, and performing transition to an optimized culture medium by using a buffer culture medium to ensure healthy and stable growth of plants; according to the method, the plant adaptability index and the culture medium deviation index are utilized, the culture medium switching risk is scientifically judged, the duration of the buffer stage is dynamically set, the preset buffer culture medium is used, the sensitivity of plants to culture medium mutation is reduced, and stress reaction caused by direct replacement is avoided.

An objective of the present invention is to provide a fluorescent compound for detecting Erwinia amylovora and diagnosing fire blight on fruit trees, as well as a method for diagnosing fire blight on fruit trees using the related compounds. [Solution] The present invention relates to a compound for diagnosing fire blight on fruit trees based on fluorescence and its use. The compound can detect Erwinia amylovora, the causative bacterium of fire blight on fruit trees, and emit red fluorescence. It has a fast diagnostic speed and high sensitivity, so it can be usefully used to diagnose fire blight on fruit trees using various and simple methods such as spraying or using a cotton swab, without requiring specialized equipment.

An objective of the present invention is to provide a fluorescent compound for detecting Erwinia amylovora and diagnosing fire blight on fruit trees, as well as a method for diagnosing fire blight on fruit trees using the related compounds. [Solution] The present invention relates to a compound for diagnosing fire blight on fruit trees based on fluorescence and its use. The compound can detect Erwinia amylovora, the causative bacterium of fire blight on fruit trees, and emit red fluorescence. It has a fast diagnostic speed and high sensitivity, so it can be usefully used to diagnose fire blight on fruit trees using various and simple methods such as spraying or using a cotton swab, without requiring specialized equipment.

The invention discloses a method for inhibiting growth of pathogenic bacteria of pyretic blight and delaying pyretic blight infection of bergamot pears by using exogenous spermine. In the research process, a bacterial solution of Erwinia amylovora is streaked and inoculated to NA culture media of exogenous polyamines with different concentrations for culture, and the growth inhibition condition of bacterial colonies is observed. The method comprises the following steps: selecting young fruits of Korla bergamot pears, sterilizing, needling and inoculating pathogenic bacteria of fire blight, then putting the young fruits into an inoculation plate paved with wet gauze, culturing, then respectively spraying equal amount of distilled water (contrast) and exogenous polyamines of different types and concentrations, continuously culturing for 2-3 days, and observing the scab formation and expansion conditions of the young fruits. Research results show that by applying the exogenous spermine with the concentration of 1.0 mmol / L, in-vitro growth of pathogenic bacteria of the fire blight can be remarkably inhibited, and the infection process of the pathogenic bacteria of the fire blight to young bergamot pear fruits can be effectively delayed. The invention provides a new technical means for green prevention and control of the fire blight of the Korla pear.

The invention discloses an aureobasidium pullulans ZX01 strain and application of the aureobasidium pullulans ZX01 strain in prevention and treatment of pear fire blight, and provides the aureobasidium pullulans ZX01 strain which is separated from leaves of siamea siamea, has the preservation number of CCTCC NO: M 20242297, can remarkably inhibit Erwinia amyloliquefaciens, and provides a new candidate strain for biological prevention and treatment of pear fire blight. The aureobasidium sp. Has a relatively strong inhibition effect on pear fire disease pathogenic bacteria, has an excellent biocontrol effect on preventing and treating pear fire disease, is environment-friendly in biological source, small in influence on the ecological environment, simple in culture condition and suitable for industrial fermentation production, and has a very good development and application prospect.

The present invention relates to a method for detecting the fire blight resistance trait of apples using a kit. The kit includes a mixed primer; the mixed primer includes a forward specific primer F-resistant, a forward specific primer F-susceptible, and a common reverse primer R; the detection method includes: extracting the genomic DNA of apple leaves; performing PCR amplification, detecting the fluorescence signal during the amplification using a fluorescence quantitative PCR instrument ABI-Q6 Flex to perform genotyping; and determining the fire blight resistance trait of apples. Using this method, apple fire blight resistance resources can be rapidly screened at the molecular level with high accuracy.

The invention relates to the technical field of agricultural biology, and particularly provides an RPA-LFD primer group and method for field on-site visual detection of pear fire blight bacteria and application of the RPA-LFD primer group and method. The primers comprise an upstream primer EaF, a downstream primer EaR and a probe Ea-pro, and the method comprises the following steps: preparing a pear fire blight sample leachate as a reaction template, carrying out an RPA-LFD reaction based on the primer group, and judging a detection result according to whether a quality control line (C) and a detection line (T) on an LFD test strip are carried or not. According to the method, DNA does not need to be extracted, high universality and specificity are achieved, the detection sensitivity can reach 5 * 10 <-6 > ng / mu L and 1 * 10 < 2 > CFU / mL, and compared with an existing rapid detection method, the detection sensitivity is remarkably improved. Technical support is provided for rapid and accurate field diagnosis, bacterial detection of plant propagation materials and evaluation of disease prevention and control effects.

The invention discloses a quorum sensing inhibitor and application of the quorum sensing inhibitor in prevention and treatment of pear fire disease pathogenic bacteria, the effective component of the quorum sensing inhibitor is 9 amino-1, 2, 3, 4,-tetrahydroacridine, by applying the quorum sensing inhibitor provided by the invention, the inhibition rate of biofilm generation of the pear fire disease pathogenic bacteria can reach 93.4%, and the inhibition rate of exopolysaccharide formation can reach 66.4%; the quorum sensing inhibitor provided by the invention has obvious inhibition activity on quorum sensing of pear fire disease pathogenic bacteria, and the activity of extracellular enzymes related to pathogenic bacteria infection, including protease, cellulase, xylanase, pectate lyase and polygalacturonase, is obviously reduced, so that the quorum sensing inhibitor provided by the invention has obvious inhibition activity on quorum sensing of the pear fire disease pathogenic bacteria, and can be used for preparing the quorum sensing inhibitor for pear fire disease pathogenic bacteria by reducing expression of virulence factors of the quorum sensing inhibitor. Therefore, the infection process is delayed, the infectivity of pear fire blight is reduced, and the damage to plants is reduced. According to the technical scheme provided by the invention, an innovative path from'pathogenic mechanism analysis' to'ecological adaptability intervention 'is opened up for comprehensive prevention and control of pear fire blight.

Disclosed herein are methods and compositions for use in controlling citrus greening, citrus canker, or fire blight, wherein the compositions comprise one or more antimicrobial peptides, or one or more polynucleotides encoding for the one or more antimicrobial peptides.

The present invention relates to new bacteriophages with SEQ ID NO.: 1-2 against Erwinia amylovora infections and use of same for treating and / or preventing an Erwinia amylovora infection in a crop.

The invention discloses an AHL-lactonase high thermal stability mutant and application thereof in biological control of fruit tree fire blight, the mutation site of the mutant is obtained by mutating the amino acid sequence of AHL-lactonase as shown in SEQ ID NO.3 from the 176th amino acid sequence to the 181th amino acid sequence from the amino terminal to the carboxyl terminal, the mutated amino acid sequence is as shown in SEQ ID NO.1, the mutant is marked as a mutant A19-9, and the mutant A19-9 has a nucleotide sequence shown in SEQ ID NO.1. The amino acid sequence from the amino terminal to the carboxyl terminal is mutated, or the amino acid sequence from the 176th site to the 184th site from the amino terminal to the carboxyl terminal is mutated, the mutated amino acid sequence is shown as SEQ ID NO.2 and is marked as the mutant C13-1, and the thermal stability of the two mutants is greatly improved compared with that of the wild type. The two mutants are applied to preparation of a biological pathogenic bacterium inhibitor, the effect of inhibiting apple fire blight caused by Erwinia amylovora infection is better than that of a wild type, and a potential biological control agent is provided for fruit tree fire blight caused by Erwinia amylovora infection.

The utility model discloses a pear fire blight sample collecting device which comprises a supporting cylinder, one end of the supporting cylinder is fixedly connected with a rope collecting assembly, the rope collecting assembly is fixedly connected with one end of an operation rope, the other end of the supporting cylinder is sleeved with a telescopic rod in a sliding mode, and the end, away from the supporting cylinder, of the telescopic rod is fixedly sleeved with a supporting base. The middle of each transmission strip is hinged to one end of a driving connecting rod through a through opening, the other end of each driving connecting rod is hinged to a collecting assembly, and the collecting assembly is slidably connected with a telescopic rod. A spherical structure is formed by the transmission strips of an arc-shaped structure, then a collection position is wrapped and isolated, a collected sample is prevented from being in contact and contamination with other parts of a host plant or other hosts, and after the transmission strips form the spherical structure, flowers, fruits or leaf parts of the host plant are cut off through the cutter fixedly connected with the end part, so that the collection sample is collected. The collected sample falls into the spherical structure, so that the sample can be conveniently collected.

The present application relates to a Paenibacillus polymyxa strain Y31, a microbial inoculum prepared therefrom and applications thereof. The strain Y31 can effectively inhibit the growth of Dothiorella gregaria, and field control tests show that the comprehensive prevention and control effect of the microbial inoculum prepared from the strain Y31 on the disease is as high as 72% or more in the early stage of the disease. Antagonistic experiments show that the strain Y31 also has good bacteriostatic effect on Dothiorella septospora and Dothiorella needle red rot fungus, and is suitable for being used as a broad-spectrum antibacterial agent in the biocontrol practice of pine fungal diseases. Meanwhile, the strain Y31 also has obvious inhibitory effect on bacterial diseases such as willow bacterial wilt fungus, pear fire blight fungus, walnut bacterial black spot fungus and kiwi fruit canker fungus, and can be used for the prevention and control of diseases such as willow wilt. In addition, the strain Y31 also has functions such as nitrogen fixation, phosphorus and potassium solubilization, and has certain salt and alkali tolerance, and can be used for promoting growth and disease resistance of plants in saline-alkali land.