46 results about "Fire blight" patented technology

The invention discloses bacillus amyloliquefaciens and application of the bacillus amyloliquefaciens in blocking of root infection of pear fire blight bacteria, the bacillus amyloliquefaciens is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.28054, and the preservation date is July 28, 2023. The bacillus amyloliquefaciens has the advantages that the bacillus amyloliquefaciens can be applied to prevention of root infection of pear fire blight bacteria; the bacillus amyloliquefaciens provided by the invention solves the problems of trunk morbidity and tree withering caused by pear fire blight self-root infection.

The invention relates to the field of biological prevention and treatment, in particular to a compound agent for preventing and treating pear fire blight as well as a preparation method and application of the compound agent. The invention provides a compound agent taking bacillus velezensis FX1 fermentation liquor and kasugamycin aqueous solution as effective components, and the compound agent can be used for effectively preventing and treating pear fire blight of fruit trees. According to the compound agent, the bacillus velezensis FX1 fermentation liquor and the kasugamycin water aqua are combined for use, so that a synergistic interaction effect is achieved, the advantages of high efficiency and rapid sterilization of the kasugamycin can be exerted, the colonization quantity of the bacillus velezensis FX1 on the surface of a tree body is increased, a phyllospheric dominant flora is formed, a stable and balanced micro-ecological system is constructed, and the growth of the tree body is promoted. The application amount of chemical pesticides can be reduced by 60%, the advantages of bacterium-pesticide synergistic complementation and disease prevention effect improvement are exerted, and the purpose of safely, efficiently and sustainably preventing and controlling occurrence, prevalence and spreading of diseases is achieved.

The present disclosure relates to a phosphor compound for diagnosing fire blight in fruit trees based on fluorescence and use thereof. The compound is capable of selectively detecting Erwinia amylovora, the causal pathogen of fire blight in fruit trees, and emits red fluorescence in response. Due to its rapid diagnostic response and high sensitivity, the compound can be effectively used for diagnosing fire blight in fruit trees through various simple methods such as spraying or swabbing, without the need for specialized equipment.

The invention relates to the technical field of plant disease-resistant gene identification and genetic engineering, in particular to a pyrus betulaefolia lectin receptor-like kinase gene sequence and application of the pyrus betulaefolia lectin receptor-like kinase gene sequence to improvement of apple and pear rot disease resistance. The pyrus betulaefolia canker-resistant gene sequence provided by the invention is used for improving the ability of resisting saprophytic fungus canker. The pyrus betulaefolia anti-disease gene provided by the invention is obtained by cloning from a pyrus betulaefolia L-LecRKs family for the first time, the function and possible action mechanism of the anti-disease gene PbeL-LecRK-S.4 in the aspect of disease resistance are further studied, and a theoretical basis is provided for breeding of pyrus betulaefolia for disease resistance. According to the invention, a pFGC5941-PbeL-LecRK-S.4 overexpression vector and a PTRV2-PbeL-LecRK-S.4 silence vector are constructed for the first time, and the pFGC5941-PbeL-LecRK-S.4 overexpression vector and the PTRV2-PbeL-LecRK-S.4 silence vector are introduced into pyrus betulaefolia ('Duli-G03') cells through transient expression verification of Huangguan pear fruits and agrobacterium tumefaciens-mediated transformation. The disease-resistant response of a pyrus betulaefolia disease-resistant gene PbeL-LecRK-S.4 overexpression suspension cell line and wild pyrus betulaefolia suspension cells after inoculation of the rot disease is studied. The pyrus betulaefolia anti-disease gene PbeL-LecRK-S.4 provided by the invention can obviously improve the resistance of pyrus betulaefolia to valsa ceratosperma.

The invention discloses a method for preventing and treating pear fire blight spots on a trunk of bergamot pears, which comprises the following steps: preparation of a medicament combination liquid: diluting bacillus amyloliquefaciens with clear water by 300-400 times, diluting multi-manganese zinc with clear water by 400-500 times, mixing uniformly diluted medicaments according to the weight ratio of bacillus amyloliquefaciens to multi-manganese zinc of (1-1.5): (1-1.5), and preparing a medicament mixed liquid for later use; when the trunk is attacked in June every year, aiming at pear fire disease spots on the trunk of the bergamot pear, sawing off diseased bark at the pus drainage part of the trunk by using an electric saw until the xylem of the trunk is not damaged; dipping the prepared medicament mixed solution by using a brush, uniformly smearing scabs, and smearing for the second time after one week. The method is simple, convenient and rapid, low in cost and high in operability, the control effect can reach 100% under the condition that the tree body has withered symptoms due to the disease spots on the trunk, and the control effect can be about 75% when the tree body has withered symptoms is gradually recovered or sprouts and shoots are formed.

The application discloses a method for preventing and treating trunk pear fire blight spots of fragrant pear, which comprises the following steps: a medicament mixed solution is prepared by mixing bacillus amyloliquefaciens diluted 300-400 times with water and manganese zinc oxide diluted 400-500 times with water in a weight ratio of 1-1.5:1-1.5; when the trunk of the fragrant pear is diseased in June every year, diseased tree bark at a trunk pus flow is cut off by using an electric saw without damaging the trunk wood; the prepared medicament mixed solution is evenly applied to the disease spots by using a brush, and the second application is performed after one week. The method is simple, fast, low in cost and high in operability, the prevention effect can reach 100% when the trunk disease spots have not caused the trunk to appear the dead symptoms, and the prevention effect can reach about 75% when the trunk has appeared the dead symptoms, the trunk can gradually recover, new buds can be generated, and new branches can be formed.

The present invention discloses a method for controlling pear tree pests and diseases using a combination of predatory mites and Bacillus amyloliquefaciens. The predatory mite is Neoseiulus barkeri. The method comprises the following steps: 1) preparing biocontrol bacteria: preparing a Bacillus amyloliquefaciens powder; 2) preparing predatory mites: preparing adult Neoseiulus barkeri mites in a matrix material; 3) preparing a mixed biocontrol product: mixing the Bacillus amyloliquefaciens powder with the matrix material containing Neoseiulus barkeri mites before use; the mixed biocontrol product is sealed in a package and stored at 13-17°C; 4) applying the agent: applying the mixed biocontrol product during the second physiological fruit drop period of the pear tree, opening the package and securing it to the trunk of the pear tree; the mixed biocontrol product used per pear tree contains 160-190 million CFU of Bacillus amyloliquefaciens and 2,900-3,100 Neoseiulus barkeri mites. The method can simultaneously control pear mite pests and pear fire blight, achieving a fire blight control efficacy of over 80%.

The invention relates to the technical field of agricultural biology, and particularly provides a method for detecting pear fire blight viable bacteria based on PMAxx-qPCR and application. By optimizing the treatment concentration and exposure time of PMAxx and combining a qPCR method, the pear fire blight viable bacteria are quantitatively detected. The method can effectively eliminate dead bacteria interference in a specific range, and is suitable for accurate quantification of the viable count in a bacterial liquid concentration range of 10 < 3 >-10 < 8 > cfu / mL. The detection method has the advantages of high specificity, high accuracy, high universality, high sensitivity and the like.

The subject of the invention is new strain Pantoea allii T14 / 15 strain deposited in the Polish Collection of Microorganisms PCM at the Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences named after Lud-wik Hirszfeld in Wroc aw. The subject of the invention is also the use of the new strain Pantoea allii T14 / 15 as agent for protecting vegetable, fruit, agricultural, and ornamental plants against fungal diseases such as white rot, gray mold, alternariosis, anthracnose, brown rot, bull's eye rot, powdery mildew and blue mold, as well as bacterial diseases such as fire blight, soft rot, affecting cultivated plants, both during the growing season and during the storage of crops. Strain Pantoea allii T14 / 15 is applied in the form of an aqueous suspension to the plant's surface, through spraying, soaking plants or their parts under storage conditions, under cover, and in field conditions, and the cell count of the strain in the suspension is at least 107 CFU / ml.

The invention discloses a rapid and efficient pear and apple canker resistance evaluation method, and relates to the technical field of horticultural plant disease resistance evaluation.The method comprises the following steps of S1, test branch preparation, S2, pathogenic bacterium culture, S3, contact method inoculation and S4, resistance evaluation.The method can accurately evaluate the canker resistance of pear and apple resources, and the method has the advantages of being high in practicability and high in practicability. The method has the advantages of simple operation, no need of punching or preparation of fungus cakes, simplified operation steps, completion of inoculation of 100-120 parts of materials within 2 hours, efficiency more than 10 times of that of a traditional punching method, suitableness for rapid screening of large-scale germplasm resources, suitableness for canker resistance evaluation of different varieties of pears and apples, germplasm resources and filial generations, and wide application prospect. And a technical support is provided for disease-resistant variety breeding, resistance gene mining and green prevention and control.

Disclosed herein are methods and compositions for use in controlling citrus greening, citrus canker, or fire blight, wherein the compositions comprise one or more antimicrobial peptides, or one or more polynucleotides encoding for the one or more antimicrobial peptides.

Disclosed herein are methods and compositions for use in controlling citrus greening, citrus canker, or fire blight, wherein the compositions comprise one or more antimicrobial peptides, or one or more polynucleotides encoding for the one or more antimicrobial peptides.

The present invention provides the application of a pear short peptide PbePEP4 in plant disease prevention and control, specifically the application of a pear (Pyrus betulifolia) short peptide PbePEP4 in the prevention and control of apricot fire blight, rapeseed sclerotinia and watermelon gray mold. The present invention artificially synthesizes the short peptide PbePEP4 for the first time, clarifies its stimulating effect on the resistance to the above-mentioned diseases, and provides a method of spraying pears, rapeseed and watermelon to stimulate their resistance to fire blight, sclerotinia and gray mold, thereby preventing and controlling the above-mentioned diseases. The PbePEP4 short peptide provided by the present invention is a product synthesized by the plant itself. The use of the short peptide to stimulate immunity and thus prevent and control diseases has the advantages of being natural, green, environmentally friendly, and not inducing pathogens to develop drug resistance. In addition, the short peptide is easy to synthesize artificially, is easy to obtain, has a strong disease resistance stimulating effect, and requires a low concentration. It is a new type of ecological and environmentally friendly crop disease prevention and control preparation.

The present invention discloses a highly thermostable mutant of AHL-lactonase and its application in biological control of fire blight of fruit trees. The mutation site of the mutant is to mutate the amino acid sequence from position 176 to position 181 of the AHL-lactonase shown in SEQ ID NO.3 from the amino terminus to the carboxyl terminus. The mutated amino acid sequence is shown in SEQ ID NO.1, denoted as mutant A19-9, or to mutate the amino acid sequence from position 176 to position 184 from the amino terminus to the carboxyl terminus. The mutated amino acid sequence is shown in SEQ ID NO.2, denoted as mutant C13-1. The thermostability of both mutants has been greatly improved compared to the wild type. When the two mutants are applied to prepare a biological pathogenic bacteria inhibitor, the inhibitory Erwinia amylovora effect on fire blight of apple caused by infection is better than that of the wild type, and it provides a potential biological control agent for E.amylovora fire blight of fruit trees caused by infection.

The application discloses a microbial source bactericidal composition containing Leuconostoc mesenteroides WZ-44 and kasugamycin and application thereof. The bactericidal composition takes Leuconostoc mesenteroides WZ-44 fermentation liquor (or fermentation sterile filtrate) and kasugamycin as main effective components, wherein the mass ratio of the Leuconostoc mesenteroides WZ-44 fermentation liquor (or fermentation sterile filtrate) to the kasugamycin is 4999:1-99:1, and is further preferably 1499:1-499:1. The bactericidal composition shows a synergistic effect within the above-mentioned ratio range, overcomes and delays drug resistance of pathogenic bacteria, has a good prevention and control effect on pear fire blight, a long effective period and low application cost, is obviously superior to a single agent in the prevention and control effect, and greatly reduces the use amount of the kasugamycin.

The invention discloses a method for preparing a bioenzyme preparation for inhibiting and promoting growth of crops and its application. The invention separates and screens a new strain of Bacillus velezensis F41‑141 from rhizosphere soil of cotton fields in Hami, Xinjiang, and obtains a bioenzyme preparation for inhibiting and promoting growth by fermenting and metabolizing the strain. The bioenzyme preparation has a control effect on cotton Verticillium wilt, with a control effect of 78.61%. It also has an inhibitory effect on cotton wilt pathogens, tomato root rot pathogens, pear fire blight and red date black spot, and is not easy to produce drug resistance, has good drug efficacy persistence, is safe for humans and animals, and has no environmental pollution problems; at the same time, the bioenzyme preparation for inhibiting and promoting growth can also improve the yield of cotton, and has the potential to promote the growth of crops as an organic fertilizer.

The invention provides an application of a pear oligopeptide PbeRALF317 in prevention and control of plant diseases, in particular to an application of a pyrus betulifolia oligopeptide PbeRALF317 in prevention and control of pear fire blight, sclerotinia rot of colza and gray mold of watermelons and grapes. The PbeRALF317 oligopeptide is artificially synthesized, the excitation effect of the PbeRALF317 oligopeptide on the resistance of the diseases is illustrated, and pear, rape, watermelon and grape are sprayed to excite the pear, rape, watermelon and grape to generate resistance on fire blight, sclerotiniose and gray mold respectively, so that the application of the diseases is prevented and controlled. The oligopeptide provided by the invention is a product synthesized by plants, and the oligopeptide is used for stimulating immunity so as to prevent and treat diseases, so that the oligopeptide has the advantages of being natural, green, environment-friendly, free from inducing pathogens to generate drug resistance and the like. The oligopeptide is easy to artificially synthesize, simple and convenient to obtain, strong in disease-resistant excitation effect and low in required concentration, and is a novel ecological and environment-friendly crop disease prevention and control preparation.

The invention relates to the technical field of pesticide production, and discloses a composition for preventing and treating bacterial diseases of crops. The composition is prepared from carboxin and fluazinam, the mass ratio of the fluazinam to the carboxin is (1%-50%): (5%-90%). The composition provided by the invention has relatively high activity on peach tree bacterial shothole, potato scab powder, potato black shank, solanaceae bacterial wilt, citrus canker, ginger blast, solanaceae canker, cucurbitaceae bacterial angular leaf spot, rice streak, rice bacterial leaf blight, pear fire blight, mango angular leaf spot and mango bacterial black spot; the composition has a remarkable synergistic effect and is superior to a single agent in the aspects of fast-acting property and persistence, and the use dosage and cost of the pesticide are reduced.

The invention provides a specific nucleic acid aptamer capable of being combined with pear fire blight bacteria and application of the specific nucleic acid aptamer, and the provided nucleic acid aptamer is an ssDNA nucleic acid aptamer with high affinity and high specificity to the pear fire blight bacteria and can be used for preparing biological products for preventing and treating the pear fire blight bacteria. The sequence of the nucleic acid aptamer is as shown in SEQ ID NO: 1-3. Through flow cytometry and KD determination, the aptamer provided by the invention has ultrahigh affinity and strong specificity, and can be widely applied to detection, prevention and control and mechanism research of pear fire blight bacteria.

The invention discloses a microbial aqueous solution for preventing and treating pear fire blight and a preparation method thereof, and belongs to the technical field of microbial fermentation. The preparation method comprises the following steps: carrying out co-fermentation on a compound strain constructed by two bacillus velezensis to obtain fermentation liquor, and adding an auxiliary agent with good biocompatibility to obtain a water aqua preparation. The preparation is simple and convenient in fermentation process, short in production period, low in cost, convenient and safe to use, good in environmental stability, easy to store and excellent in disease prevention effect, and a new product is provided for safe and green prevention and control of pear fire blight.

The invention discloses application of p-allylphenol in preparation of an inhibitor for preventing and treating pear fire blight and preparation of p-allylphenol, and relates to the technical field of biology. The preparation of p-allyl phenol comprises the following steps: obtaining spores of Streptomyces sp.D67, inoculating the spores to a culture medium for cultivation, and carrying out purification treatment on the obtained crude extract to obtain p-allyl phenol. The effective concentration of p-allylphenol is 0.125 mg / mL-0. 5mg / mL, and it is found that p-allylphenol has quorum sensing inhibition activity on pear fire disease pathogenic bacteria. The p-allylphenol is applied to the preparation of the inhibitor for preventing and treating pear fire blight, the inhibition rate of the p-allylphenol on the generation of a pear fire blight pathogenic bacteria biofilm reaches 69.3%, and the inhibition rate of the p-allylphenol on the formation of exopolysaccharides reaches 39.9%; the p-allylphenol has an outstanding technical effect on solving the pear fire blight, and provides a new choice for biological prevention and control application of the pear fire blight.

The invention belongs to the technical field of prevention and treatment of bacterial diseases, and particularly relates to application of disulfide in prevention and treatment of pear fire blight. According to the present invention, the disulfide can strongly inhibit the T3SS of the pear fire blight bacteria while the growth of the pear fire blight bacteria is not affected, such that the pathogenicity of the pathogenic bacteria is significantly reduced, and the effect of treating the pear fire blight caused by the pear fire blight bacteria is achieved while the growth of the pear fire blight bacteria is not affected. Therefore, the disulfide or the pharmaceutically acceptable salt thereof can be used as a pathogenic bacterium T3SS inhibitor, can be prepared into a medicament for preventing and / or treating the pear fire blight, and also has the effect of reducing and delaying the drug resistance of the pear fire blight to the compound, so that the disulfide or the pharmaceutically acceptable salt thereof can be used for preparing the medicament for preventing and / or treating the pear fire blight. The effective service life is relatively long in the aspect of preventing and / or treating pear fire blight, and the application prospect is wide.

The present invention relates to means and methods comprising Paraburkholderia bacteria for use in preventing and / or controlling plant pests, in particular Erwinia amylovora which constitutes the causative agent of fire blight. The present invention thus relates to novel non-medical uses of Paraburkholderia bacteria for preventing and / or controlling pests on a plant and / or on a temporary part of a plant and / or being located inside of a plant. Furthermore, the present invention relates to methods and compositions comprising Paraburkholderia bacteria for preventing and / or controlling such pests on a plant and / or on a temporary part of a plant and / or being located inside of a plant as well as to the use of Paraburkholderia bacteria for the preparation of such compositions. Further provided are kits comprising components used in the methods and compositions of the invention. The inventive use of Paraburkholderia bacteria for the prevention and / or control of plant pests are, without being limiting, particularly useful in commercial crop protection campaigns, particularly against the causative agent of fire blight. Erwinia amylovora.

The invention discloses an application of a pear fire blight bacterium effect factor HrpG in prevention and control of pear fire blight. A nucleotide sequence of the effect factor HrpG gene is shown as SEQ ID NO: 1. Through a gene editing technology, the pyraclospermum palmatum mutant delta HrpG of which the effector HrpG gene is knocked out is constructed. Korla young fruits and pyrus betulaefolia in-vitro leaves are inoculated through a control group of wild fire blight bacteria and delta HrpG, and the delta HrpG is found to be slower in morbidity and smaller in scabs; the mutant delta HrpG is applied to plants or a plant growth environment, and biological prevention and control of fire blight are achieved through the competitive effect of the mutant delta HrpG and wild type pathogenic bacteria. Therefore, delta HrpG is weaker in pathogenicity, and provides a new strategy for green prevention and control of pear fire blight in application.

The invention discloses a pear fire and blight resistant pear stock tissue culture environment control method, and particularly relates to the technical field of plant tissue culture, which comprises the following steps: acquiring plant tissue metabolism and growth data, inputting the data into a pre-trained machine learning model, and outputting a result whether a culture medium needs to be replaced or not; when the culture medium needs to be replaced, the model predicts and optimizes a component parameter group of the culture medium; performing feature extraction and analysis on the optimized culture medium parameter group and the current culture medium parameter group to generate culture medium deviation and plant adaptability data; based on an analysis result, determining the duration of a buffer stage through buffer judgment, and performing transition to an optimized culture medium by using a buffer culture medium to ensure healthy and stable growth of plants; according to the method, the plant adaptability index and the culture medium deviation index are utilized, the culture medium switching risk is scientifically judged, the duration of the buffer stage is dynamically set, the preset buffer culture medium is used, the sensitivity of plants to culture medium mutation is reduced, and stress reaction caused by direct replacement is avoided.

The present invention discloses a Desmospora profundinema GS1 strain. In a plate confrontation test, it was found that the GS1 strain has the activity of inhibiting the growth of multiple plant pathogenic bacteria, and the maximum diameter of the inhibition zone can reach 26 mm; in a potted inoculation test, it was found that the GS1 strain can inhibit the infection of citrus canker bacteria. The GS1 strain of the present invention also has a high efficiency inhibitory effect on pathogens such as peach root cancer, tobacco bacterial wilt, rice white leaf blight, apricot fire blight, melon bacterial fruit spot, and Chinese cabbage soft rot. The Desmospora profundinema of the present invention has a high efficiency antibacterial activity against plant pathogenic bacteria, and therefore, the Desmospora profundinema GS1 can be used as a biocontrol bacterium in the prevention and treatment of plant bacterial diseases.

An objective of the present invention is to provide a fluorescent compound for detecting Erwinia amylovora and diagnosing fire blight on fruit trees, as well as a method for diagnosing fire blight on fruit trees using the related compounds. [Solution] The present invention relates to a compound for diagnosing fire blight on fruit trees based on fluorescence and its use. The compound can detect Erwinia amylovora, the causative bacterium of fire blight on fruit trees, and emit red fluorescence. It has a fast diagnostic speed and high sensitivity, so it can be usefully used to diagnose fire blight on fruit trees using various and simple methods such as spraying or using a cotton swab, without requiring specialized equipment.

An objective of the present invention is to provide a fluorescent compound for detecting Erwinia amylovora and diagnosing fire blight on fruit trees, as well as a method for diagnosing fire blight on fruit trees using the related compounds. [Solution] The present invention relates to a compound for diagnosing fire blight on fruit trees based on fluorescence and its use. The compound can detect Erwinia amylovora, the causative bacterium of fire blight on fruit trees, and emit red fluorescence. It has a fast diagnostic speed and high sensitivity, so it can be usefully used to diagnose fire blight on fruit trees using various and simple methods such as spraying or using a cotton swab, without requiring specialized equipment.

The invention discloses a method for inhibiting growth of pathogenic bacteria of pyretic blight and delaying pyretic blight infection of bergamot pears by using exogenous spermine. In the research process, a bacterial solution of Erwinia amylovora is streaked and inoculated to NA culture media of exogenous polyamines with different concentrations for culture, and the growth inhibition condition of bacterial colonies is observed. The method comprises the following steps: selecting young fruits of Korla bergamot pears, sterilizing, needling and inoculating pathogenic bacteria of fire blight, then putting the young fruits into an inoculation plate paved with wet gauze, culturing, then respectively spraying equal amount of distilled water (contrast) and exogenous polyamines of different types and concentrations, continuously culturing for 2-3 days, and observing the scab formation and expansion conditions of the young fruits. Research results show that by applying the exogenous spermine with the concentration of 1.0 mmol / L, in-vitro growth of pathogenic bacteria of the fire blight can be remarkably inhibited, and the infection process of the pathogenic bacteria of the fire blight to young bergamot pear fruits can be effectively delayed. The invention provides a new technical means for green prevention and control of the fire blight of the Korla pear.

The invention discloses an aureobasidium pullulans ZX01 strain and application of the aureobasidium pullulans ZX01 strain in prevention and treatment of pear fire blight, and provides the aureobasidium pullulans ZX01 strain which is separated from leaves of siamea siamea, has the preservation number of CCTCC NO: M 20242297, can remarkably inhibit Erwinia amyloliquefaciens, and provides a new candidate strain for biological prevention and treatment of pear fire blight. The aureobasidium sp. Has a relatively strong inhibition effect on pear fire disease pathogenic bacteria, has an excellent biocontrol effect on preventing and treating pear fire disease, is environment-friendly in biological source, small in influence on the ecological environment, simple in culture condition and suitable for industrial fermentation production, and has a very good development and application prospect.