38 results about "Fire blight" patented technology

The invention discloses a Brevibacillus laterosporus strain N106-2 of which the collection number is CGMCC No.8482. The strain has strong indoor flat inhibiting actions on multiple plant pathogenic fungi (fussarium wilt of tomato, root rot of chili, gray mold of tomato, Rhizoctonia solani, black spot of cabbage, anthracnose of pear and black rot of pear) and plant pathogenic bacteria (bacterial wilt of tomato, fire blight of pear and streak of rice); and the strain can secrete proteinase, cellulase and surfactant antimicrobial related substances with hemolytic activity. The Brevibacillus laterosporus can be prepared into a biocontrol aqua; the biocontrol aqua has certain preventing and treating effects on fussarium wilt of tomato, fusarium rot of chili, anthracnose of pome and eyespot of pome. The Brevibacillus laterosporus N106-2 has the advantages of no toxicity, no pathogenicity and high safety for human and livestock, is environment-friendly, is a useful resource for development of microbial pesticides, and has potential application prospects.

A process for increasing the sensitivity of RCR technique in detecting the pathogenic bacteria of plant features that the fire blight bacterium of pear is used as target, and includes such steps as preparing the antiserum specific to pathogenic bacterium, coating PCR reaction tube, washing the PCR reaction tube, adsorbing the target bacteria, and PCR amplifying. It can increase the sensitivity by 1000 times.

A new and distinct variety of apple tree rootstock Malus domestica×Malus robusta hybrid ‘G.41’ is described herein. The new variety is a dwarfing rootstock that is resistant to fire blight (Erwinia amylovora) and crown rot (Phytophthora cactorum). The ‘G.41’ rootstock is useful in that it can be propagated clonally and used as a rootstock or root system for apple trees as well as for interstems of apple trees.

The invention relates to a test kit of Asian erwinia amylovora, belonging to the category of preventing and treating diseases of crops as well as plant quarantine. The upstream primer is HrcC-F1:5'-GACGTTTGCACCTGGAAACCG-3', and the downstream primer is HrcC-R1:5'-GCGGTAGGTAATCAAGGCCAC-3'. The kit comprises 1mL of detecting solution, containing 50mmol.L Tris.Cl, 125mmol.L KCl, 2.5mmol.L MgCl, 10mMd NTPs, 0.25mmol/mL of upstream and downstream primers, 0.1mg.mLBSA, and 100 units of Taq DNA polymerase. Based on the primers, the test kit of Asian erwinia amylovora has higher specificity, sensitivity and stability, so as to provide a fast, sensitive and special technical method for testing the Asian erwinia amylovora. The products of special PCR of the Asian erwinia amylovora are shown in the figure.

The invention discloses a 2, 4-dioxoimidazoline cyclohexane sulfonamide compound, which has a structure as shown in the following general formula (1). In the formula, R is selected from any one of C1-C20 alkyl, substituted or unsubstituted benzyl, substituted or unsubstituted phenethyl, 2-thiopheneethyl, 2-pyridylethyl and morpholine-N-ethyl. The 2, 4-dioxoimidazoline cyclohexane sulfonamide compound provided by the invention has an inhibition effect on fungi such as botrytis cinerea, sclerotinia sclerotiorum, phytophthora capsici, rice sheath blight, rice blast and fusarium graminearum, and is used for preventing and treating diseases of the fungi; the compound has an inhibiting effect on bacteria such as cabbage soft rot, citrus ulcer, pepper scab, pear fire blight and tomato ulcer, andis used for preventing and treating diseases.

A new and distinct variety of apple tree rootstock Malus domestica×Malus robusta hybrid ‘G.935’ is described herein. The new variety is a dwarfing rootstock that is resistant to fire blight (Erwinia amylovora) and crown rot (Phytophthora cactorum). The ‘G.935’ rootstock is useful in that it can be propagated clonally and used as a rootstock or root system for apple trees as well as for interstems of apple trees.

The invention relates to the technical field of plant protection and particularly discloses a method for measuring the pathogenicity of pear fire blight bacteria by means of an in-vitro water cultureapproach for fragrant pear branches. The method comprises the following steps of (1) preparation of a pear fire blight bacterium inoculation solution; (2) preparation of in-vitro branches; (3) inoculation; (4) observation of an inoculation result and evaluation of the pathogenicity. The method is simple and feasible, the pathogenicity of pathogenic bacteria is stably expressed, the repeatability is high, and the result is reliable; the pathogenicity and pathogenicity intensity of the pear fire blight bacteria can be accurately reflected. In actual operation, the method can be carried out in batches and has the advantages of small occupied space, lower labor cost and shorter experimental cycle. Therefore, the method is suitable for application and popularization.

The present invention discloses a klebsiella sp. and use thereof in preventing and treating of fire blight of fruit trees, and belongs to the technical field of biological prevention and treatment ofplant diseases. A TN50 strain which has a strong competitive effect and a remarkable inhibition effect on growth of erwinia amylovor is screened from endophytic bacteria separated from healthy Korla pear branches, is identified as klebsiella sp. and preserved in China General Microbiological Culture Collection Center (CGMCC) on November 18, 2020, and has the preservation number of CGMCC No.21204.A klebsiella TN50 strain fermentation broth and active metabolites in the fermentation broth have good prevention and treatment effects on fire blight of kernel fruit trees such as rosaceae pears, apples, haws, etc., and the TN50 strain is a biocontrol potential strain with a high prevention effect, good environmental safety and development and application prospects.

The invention relates to the technical field of plant disease prevention and control, in particular to application of polypeptide to preparation of a preparation for preventing and treating plant diseases; the preparation is prepared by taking polypeptide as an active component and adding auxiliary materials or auxiliary components. The preparation disclosed by the invention has obvious prevention and treatment effects on plant diseases caused by Erwinia amylovora, and has outstanding prevention and treatment effects on fire blight of rosaceae plants, especially economic crops such as pears, apples, haws and malus spectabilis. Compared with the prior art, the polypeptide preparation has the effects of wide action range, high safety, no biotoxicity, no drug resistance, no stimulation and no hormone, and can effectively prevent and treat plant diseases.

The invention relates to a real-time fluorescent PCR (polymerase chain reaction) quick detection primer and kit for pear fire blight pathogenic bacteria. The primer comprises a forward primer of which the sequence is SEQ ID NO:1 and a reverse primer of which the sequence is SEQ ID NO:2. In the invention, a specific conserved sequence is obtained through molecular biological analysis according to an ITS gene sequence of pear fire blight pathogenic bacteria, and a specific amplification primer thereof is designed. The conserved gene sequence is shared by different pear fire blight pathogenic bacterium strains to ensure the reliability in detection of pear fire blight pathogenic bacteria from different sources on a species level. Besides, the primer provided by the invention adopts fluorescence labeling; and compared with the common PCR technology, observation does not need to be performed through a gel electrophoresis method, thus realizing the integrated sealed detection in the detection process.

The present invention relates to a new and distinct variety of apple tree named ‘G.814.’‘G.814’ is useful in that it can be propagated clonally and used as a rootstock or root system for apple trees as well as for interstems of apple trees. When used as a rootstock, ‘G.814’, is: dwarfing, induces scion precocity, is cold hardy, induces scion precocity, has a high yield efficiency, is tolerant to replant disease, and is resistant to: crown and root rot (Phytophthora cactorum), fire-blight (Erwinia amylovora), and powdery mildew. ‘G.814’ is susceptible to Apple Stem Grooving Virus (ASGV) and wooly apple aphid.

The invention discloses an insecticide for preventing disease of fruit trees. The insecticide is prepared from the following raw materials: 10 to 18 parts of tobacco leaves, 15 to 20 parts of garlic,8 to 15 parts of castor leaves, 12 to 18 parts of radix sophorae flavescentis, 6 to 15 parts of Chinese prickly ash, 5 to 8 parts of polyisobutene, 30 to 40 parts of an ultraviolet absorbent, 1 to 5 parts of carbon powder, and 11 to 17 parts of hexane. The insecticide has high contact toxicity to aphid, red spiders and ants, also has a suffocating and stomach poisoning effect, and can prevent thegrub; main raw materials are natural plants, so that the insecticide is nontoxic to the human body, and environment-friendly, and has the advantages of simple processing, low processing cost, low rawmaterial acquisition cost, and wide popularization range; and by mixing the polyisobutene, ultraviolet absorbent, carbon powder, hexane and a mixed solution of a tobacco solution, a garlic solution, castor beans powder, radix sophorae flavescentis and Chinese prickly ash, the drug effect can be prevented from being influenced by the direct illumination of the sunlight ultraviolet, the agglomeration of the medicine can also be avoided, the diffusion is avoided, and the medicine effect is not influenced; the insecticide is sprayed onto tree leaves, so that the alternaria leaf spots, rust diseases and fire blight can be effectively prevented; and when the insecticide is buried at the roots, the black rot and root cancer can be prevented.

The invention discloses nested PCR reagents and a detection system for detecting pear pollen pear fire blight bacteria, the detection reagents comprise a first primer pair and a second primer pair; the nucleotide sequence of the upstream primer of the first primer pair is as shown in SEQ ID NO.1, and the nucleotide sequence of the downstream primer is as shown in SEQ ID NO. 2; and the nucleotide sequence of the upstream primer of the second primer pair is as shown in SEQ ID NO.3, and the nucleotide sequence of the downstream primer is as shown in the specification. According to the invention, the nested PCR system for high-sensitivity detection of the pear pollen branch blight bacteria is established, and the nested PCR molecular method is utilized to establish the high-sensitivity pear pollen blight bacteria detection system, so that the sterility and safety of the commercial pear pollen are ensured, and the path of propagation and diffusion of the bergamot pear branch blight bacteria through the pollen is cut off.