Application of Chickpea Extract in Controlling Crop Pathogenic Bacteria

A technology of pathogenic bacteria and extracts, applied in the agricultural field, can solve problems such as threats to human health, adverse effects on humans, animals and the ecological environment, and excessive pesticide residues.

Active Publication Date: 2021-07-02
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of pesticides has caused adverse effects on humans, animals and the ecological environment. Pesticide residues in food and the environment exceed the standard, seriously threatening human health.

Method used

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  • Application of Chickpea Extract in Controlling Crop Pathogenic Bacteria
  • Application of Chickpea Extract in Controlling Crop Pathogenic Bacteria
  • Application of Chickpea Extract in Controlling Crop Pathogenic Bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Isolation and Identification of Anti-Xag Active Substance Extracts from Chickpea Plants

[0023] Weigh 1g of chickpea plant, cut it into small pieces with a length of about 1mm with a knife, transfer it to a mortar and grind it, transfer the tissue fragments to a centrifuge tube, add 200μL of ethanol to it, and shake it in a shaker at 12000rpm for 10min , transfer the centrifuge tube to a 4°C centrifuge at 8000rpm, centrifuge for 10min, filter the supernatant through a 0.22μm filter membrane and detect it with high performance liquid chromatography. Adopt C18 chromatographic column (250mm * 4.6mm, Phenomenex), the ultraviolet detection wavelength is 254nm, with acetonitrile and water as mobile phase, the content of acetonitrile is gradually increased from 10% to 100% in the first 30min, after maintaining 5min, drop to 10% immediately and Maintain for 5 minutes, the flow rate is 1 mL / min, and the temperature of the column oven is 25°C. High performance liquid chromatogr...

Embodiment 2

[0028] Detection of antagonistic activity of biochanin A and genistein against pathogenic bacteria in crops

[0029] The crop pathogenic bacteria used in this example include Xag, Ea, Cm, and Ac. The preparation method of the pathogenic bacteria liquid is the same as in Example 1. Add 4 μL bacterial solution to 200 μL LB liquid medium containing 0 μg / mL, 50 μg / mL, 100 μg / mL, 200 μg / mL, 300 μg / mL, 400 μg / mL, 500 μg / mL chickpein A or genistein, The turbidity of the bacterial solution was monitored after 6 hours of shaking culture at 200 rpm in a shaker at 28°C. The results are shown in Table 1.

[0030] Table 1 Minimum inhibitory concentration of biochanin A and genistein to pathogenic bacteria in crops

[0031]

[0032] It can be seen from the above table that the minimum inhibitory concentration (MIC) of biochanin A to Xag is 100 μg / mL, and the MIC value to Ea, Cm, and Ac is 200 μg / mL. The MIC of genistein to Xag, Ea, Cm, and Ac is greater than 500 μg / mL. It can be see...

Embodiment 3

[0034] Effects of biochanin A on the motility of Xag flagella

[0035] Xag bacterial liquid preparation method is the same as embodiment 1. Prepare LB solid medium containing 0 μg / mL, 5 μg / mL, 12.5 μg / mL, 25 μg / mL, and 50 μg / mL chickpein A, add 3 μL of bacterial solution to the medium, and place the culture dish at 28 After culturing for 72 hours in an incubator at ℃, measure the colony diameter.

[0036] The result is as figure 2 As shown, 50 μg / mL biochanin A can significantly inhibit the flagellar mobility (Swimmingmotility) of Xag.

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Abstract

The invention discloses the application of chickpea extract in preventing and treating pathogenic bacteria of crops, and belongs to the field of agricultural technology. Specifically, the chickpea extract is biochanin A or / and genistein, and the extract is effective against soybean spot pathogen ( Xanthomonas axonopodis PV .glycines,Xag ), Phytophthora amylovora ( Erwinia amylovora, Ea ), Bacterial canker of tomato ( Clavibacter michiganensis, Cm ), Acidophage watermelon ( Acidovorax citrulli, Ac ) have inhibitory effect, and can be used to prepare pesticides for the control of pathogenic bacteria in crops.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to the application of chickpea extracts in preventing and treating pathogenic bacteria in crops. Background technique [0002] The development of agriculture is inseparable from pesticides, but while pesticides have made significant contributions to agricultural development, they have also brought harm to the natural environment. In recent years, due to the abuse of chemical pesticides, the resistance of pests has increased, leading to an increase in the amount and frequency of pesticide use, which has further aggravated environmental pollution and ecological damage, resulting in a vicious circle and seriously endangering human life and health. According to related reports, only about 5% of pesticides play their due role in the application process, while the vast majority of pesticides are lost to the surrounding environment such as water and soil, causing environmen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/20A01N43/16A01P1/00
CPCA01N43/16A01N65/00A01N65/20
Inventor 拉博德·佩德罗王苏妍徐东胡凯旋施欣驰
Owner NANTONG UNIVERSITY
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