71 results about "Bacterial canker" patented technology

The invention relates to citrus endophytic actinomycetes with antibacterial activity on various plant pathogens, belonging to the technical field of microbes. The endophytic actinomycetes are Streptomyces sp. R1020 which was registered and collected in China General Microbiological Culture Collection Center on March 17, 2014 with the collection number of CGMCC No. 8923. The strain R1020 has relatively good inhibitory effect on citrus anthrax bacteria, citrus bacterial canker disease bacteria, citrus penicillium italicum, citrus penicillium digitatum, bayberry deadwood disease bacteria, bayberry acicola, bayberry anthrax bacteria, loquat anthrax bacteria, loquat leaf spot fungi, loquat ringspot pathogenic bacteria, tomato alternaria solani, botrytis cinerea, colletotrichum orbiculare, tea tree anthrax bacteria and elsinoeleucospila and has the potential of being developed into biological pesticides.

The invention relates to a smearing preparation, in particular to a smearing preparation for preventing and controlling kiwifruit bacterial canker. Bromothalonil as a low-toxicity medicament is used as an active ingredient of the smearing preparation, and smearing is used as the using way of the smearing preparation, so that no liquid medicine drifting problem exists. More importantly, the smearing preparation provided by the invention is a gel liquid and can automatically form a uniform coating at the wound part of a kiwifruit after being used, so that infection of foreign germs to a wound of a plant and water evaporation of the plant can be effectively reduced, influences of the wound to the growth of the kiwifruit can be reduced, and the aims of preventing and controlling the kiwifruitbacterial canker for a long term can be achieved.

The invention discloses a bactericidal composition containing diacetone amine copper oxalate and triazole bactericides. The bactericidal composition comprises a first active ingredient, namely diacetone amine copper oxalate, and a second active ingredient, namely one of the triazole bactericides, wherein a weight ratio of the first active ingredient to the second active ingredient is (1:15)-(15:1), and the sum of the content of the first active ingredient and the second active ingredient is 5-90 percent of the total weight of the bactericidal composition. The composition can be prepared into agriculturally allowable forms such as water dispersible granules, wettable powder, dispersible oil suspended agents or micro emulsion. The bactericidal composition disclosed by the invention effectively solves the problem that the effect of eradicating crop diseases by singly using the diacetone amine copper oxalate or the triazole bactericides is not ideal, has an obvious synergistic effect and is high in crop safety. The bactericidal composition disclosed by the invention has good effects of controlling altermaria leaf spot and brown spot of apples, bacterial canker diseases and anthracnose of citruses, downy mildew, powdery mildew and angular leaf spot of cucurbites, false smut, sheath blight and blast of rice, and black point and leaf spot diseases of bananas.

The invention provides an anti-citrus bacterial canker disease pthA-nls gene, a construction method and application thereof. The invention uses the pathogenic gene pthA-mediated broad-spectrum disease resistance theory of the citrus bacterial canker disease to construct a new anti-citrus bacterial canker disease gene (pthA-nls). The construction method comprises the following steps of: (1) primer design; (2) clone of NLS sequence; (3) recovery and enzyme digestion of PCR product; (4) double enzyme digestion of a plant expression vector; (5) connection between product of enzyme digestion of pBI121 and corresponding product of PCR enzyme digestion of the NLS sequence by T4 DNA ligase; (6) acquisition of positive cloned gene pthA-nls by converting ligation product into colon bacillus DH5alpha. The gene pthA-nls can further perform genetic transformation on susceptible varieties of orange so as to acquire new anti-citrus bacterial canker disease germ plasm. The anti-citrus bacterial canker disease pthA-nls gene provides an effective tool for breeding of anti-citrus bacterial canker disease.

The invention provides a three-dimensional prevention and treatment method of bacterial canker of kiwifruits. The method comprises the steps that stem coating is conducted on plants in winter, pesticide and bactericide killing is conducted in time, and soil is improved and sterilized. In the stem coating stage, stem coating fertilizer is used, and the stem coating fertilizer, by mass, comprises 10% of plant oil processed through plasma treatment and 90% of polymer powder; in the real-time pesticide and bactericide killing stage, 50% of 600-800-fold tuzet wetting powder, 10% of 3500-4000-fold imidacloprid, 20% of 600-800-fold buprofezin, 10% of 1000-fold chlorfenapyr suspension liquid, 45% of 800-1000-fold ambam, 2.5% of 1000-fold efficient cyhalothrinmissible oil and water soluble fertilizer are adopted, and the water soluble fertilizer is plant oil processed through plasma treatment; in the soil modification stage, root fertilizer is adopted, and the root fertilizer, by mass, is composed of 1% of plant oil processed through plasma treatment, 90% of polymer powder and 9% of water. The three-dimensional prevention and treatment method has a remarkable bacterial canker prevention effect, and effectively prevents secondary damage and pathogen infection.

The invention discloses a fertilizer and pesticide combination reagent for preventing and killing bacterial canker of kiwifruit and a preparation method of the fertilizer and pesticide combination reagent. The fertilizer and pesticide combination reagent is prepared from the following raw materials in parts by weight: 200 parts of agricultural amino acid water soluble powder, 40-50 parts of anhydrous zinc chloride, 45-65 parts of anhydrous manganese chloride, 40-80 parts of a ciprofloxacin active compound or a ciprofloxacin active compound, 100-200 parts of a 14% methyl chloride isothiazolinone water agent, 100-150 parts of oxine-copper active compound, 0.1 part of forchlorfenuron, 0.1 part of a 5-nitroguaiacol sodium salt active compound, 0.5 part of an alpha-pimacol active compound, 350-450 parts of molasses microbial fermentation concentrate solution, 0.2-0.25 part of sodium polyacrylate powder, 50-60 parts of No.YD-7 liquid fertilizer synergia solution, 0.1-0.2 part of industrial alcohol and the balance of deionized water to supplement the raw materials to 1200-1250 parts. The fertilizer and pesticide combination reagent disclosed by the invention is a high-density liquid product with fertilizer and pesticide effects, has good affinity for both water and plants, mainly contact and kill plant bacteria, and can also kill pathogenic fungus. A layer of compact organic pesticide and fertilizer film is formed outside a tree body when the fertilizer and pesticide combination reagent is applied in large concentration, and a sustained release function is exerted.