28 results about "Bacterial canker" patented technology

The invention belongs to the technical field of genetic engineering, and relates to application of a transcription factor AcWRKY20 in resisting kiwifruit bacterial canker. Through gene cloning, phylogenetic analysis, subcellular localization, transgenic function verification and other technologies, it is proved for the first time that the transcription factor AcWRKY20 has the molecular characteristic of remarkably enhancing the disease resistance of kiwi fruits. According to the invention, the AcWRKY20 gene is respectively silenced and overexpressed and then is inoculated with Psa, and phenotypic observation shows that the gene is a disease-resistant gene and is suitable for canker-resistant breeding of kiwi fruit.

The application discloses a kiwi bacterial canker disease susceptible gene AcADC1 and application thereof, and belongs to the technical field of genetic engineering. The nucleotide sequence of the AcADC1 gene is shown in SEQ ID NO:1, and the arginine decarboxylase (ADC) synthesized by the gene is encoded, and the amino acid sequence is shown in SEQ ID NO:2, and the arginine (Arg) is responsible for catalyzing the formation of putrescine. The resistance of the kiwi plant with the silenced AcADC1 gene to the bacterial canker disease is improved, the resistance of the kiwi plant with the overexpressed AcADC1 gene to the bacterial canker disease is reduced, the AcADC1 gene plays a negative regulation role in the immune response of the kiwi to the bacterial canker disease, the gene editing technology can be used for editing the AcADC1 gene, and a theoretical basis and gene reserve are provided for kiwi disease-resistant breeding.

The present application belongs to the field of biotechnology, and particularly relates to a preparation method and application of five compounds (1-5) extracted from liquid fermentation of Chaetomium sp. Specifically, the present application discloses five compounds, wherein compound 1 is a new compound. The Chaetomium sp. metabolite has good antibacterial activity on three tested pathogenic bacteria (Pseudomonas syringae pv. actinidiae, Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola), and has important significance for development of a new type of microbial source antibacterial agent.

The application discloses a primer probe composition which is composed of a forward primer, a reverse primer and a probe primer. The sequence of the forward primer is shown as SEQ ID No. 1, the sequence of the reverse primer is shown as SEQ ID No. 2, and the sequence of the probe is shown as SEQ ID No. 3. The application establishes a detection method for Pseudomonas syringae pv. actidii, which comprises the following steps: extracting genomic DNA of a sample to be detected, taking the genomic DNA as a template, and performing RAA detection by using the primer composition; and determining whether the sample to be detected contains Pseudomonas syringae pv. actidii according to a strip of a LFD test paper. The detection method provided by the application has a minimum detection sensitivity of 1 pg / µL for Pseudomonas syringae pv. actidii DNA, and has a minimum detection limit of 1×10 3 cfu / mL for a bacterial suspension, and can provide a new technical means for early diagnosis and disease prevention and control of bacterial canker disease of kiwifruit.

A new cherry tree particularly distinguished dark red-purple fruit with a kidney to flat-round shape, a precocious fruiting habit, self-fertility, good resistance to rain crack and moderate resistance to bacterial canker, is disclosed.

A new cherry tree particularly distinguished dark red fruit with a flat-round shape, a very precocious fruiting habit, self-fertility, very tolerant to rain crack and moderate resistance to bacterial canker, is disclosed.

A new cherry tree particularly distinguished self-fertility, resistance to rain crack and resistance to bacterial canker, is disclosed.

The invention belongs to the technical field of microorganisms, and discloses bacillus velezensis with induced resistance activity and application of the bacillus velezensis in plant disease control. According to the bacillus velezensis H-33 and the application thereof, the bacillus velezensis H-33 is separated and screened from healthy kiwi fruit branches, the strain can improve the disease resistance of plants by inducing the systemic resistance of the plants, and the bacillus velezensis H-33 has a prevention and treatment effect on plant diseases such as kiwi fruit bacterial canker, pear tree canker, apple tree canker and apple anthracnose, and can be used for preventing and treating the plant diseases such as kiwi fruit canker, pear tree canker, apple tree canker and apple anthracnose. The broad-spectrum antibacterial activity is realized. The invention provides a new strain resource for green prevention and control of plant fungal diseases and plant bacterial diseases, and has a good application prospect.

This invention belongs to the field of biotechnology. To improve the effectiveness of biological control of plant diseases, this invention discloses *Bacillus velezensis* ZF516 and its application in controlling various soil-borne diseases. The *Bacillus velezensis* strain number is ZF516, and its registration number at the China General Microbiological Culture Collection Center is CGMCC No. 30136. This *Bacillus velezensis* strain can prevent and / or treat tomato bacterial canker, tomato bacterial wilt, or cowpea root rot, or can be used to prepare products for the prevention and / or treatment of these diseases. This strain also exhibits good inhibitory effects against *Fusarium oxysporum*, *Diplostomum spp.*, *Rhizoctonia solani*, *Fusarium solanum*, *Corynebacterium multiflorum*, *Botrytis cinerea*, *Agrobacterium tumefaciens*, *Pectinobacter brasiliensis*, *Ralstonia solanacearum*, *Streptomyces spp.*, etc.

A new cherry tree particularly distinguished self-fertility, resistance to rain crack and resistance to bacterial canker, is disclosed.

The invention belongs to the technical field of molecular biology and genetic breeding, and discloses a molecular marker related to a kiwifruit canker resistance Psa8-246 site and application thereof. The canker-resistant site Psa8-246 located on the No.8 chromosome of the kiwi fruit is found through genome-wide association analysis, the canker-resistant site Psa8-246 is located at the 2077786th basic group of the No.8 chromosome of a reference genome of the kiwi fruit Red5, and the phenotypic variation rate of 5.3% can be explained. When 155 kiwi fruit samples are detected by using the KASP marker designed by the SNP, the KASP marker is found to be simple and convenient to operate and clear in typing, and has a good selection effect on the kiwi fruit canker.

A new cherry tree particularly distinguished by red, flat, round fruit, with a highly precocious fruiting habit, self-fertility, less susceptible to rain crack and moderate resistance to bacterial canker, is disclosed.

A new cherry tree particularly distinguished self-fertility, resistance to rain crack and resistance to bacterial canker, is disclosed.

The application discloses a kind of active substances for crop protection and its composition and preparation method and application, the substance general formula as follows: Wherein: R1 It is hydrogen atom, halogen, cyano, methyl, methoxy, nitro, trifluoromethyl or the disubstituted of arbitrary combination of above halogen;R2 It is hydrogen atom, halogen or nitro substitution.This application derivative shows better inhibitory effect to rice bacterial leaf spot, rice bacterial leaf blight, kiwi bacterial canker, citrus canker.The inhibitory activity of compound II-18 to kiwi bacterial canker bacteria is best.Plant pathogenic fungi (sclerotinia sclerotiorum, phomopsis viticola, botryosphaeria dothidea and fusarium graminearum) inhibitory activity test shows that compound II-10 shows better inhibitory activity to fusarium graminearum.In addition, part of the derivatives of the application has significant contact toxicity to bactrocera dorsalis (Hendel).The composition of compound II-5 and methyl eugenol shows synergistic trapping effect in the process of preventing and treating the main pest of kiwi, bactrocera dorsalis (Hendel).

The invention belongs to the technical field of microorganisms, and discloses a preparation method and application of a biological agent for preventing and treating kiwifruit bacterial canker, the biological agent comprises the following components by mass: LY2-3 fermentation liquor, a carrier, a stabilizer, a protective agent, a dispersant and a wetting agent, the LY2-3 fermentation liquor is bacillus cereus LY2-3 fermentation liquor, and the carrier is bacillus cereus LY2-3 fermentation liquor. The optimal mass percentage of each component is as follows: 70% of mother powder formed by mixing LY2-3 fermentation liquor and diatomite, 2% of calcium carbonate, 2.5% of dextrin, 10% of sodium lignin sulfonate, 10% of nekal and the balance of diatomite. The biological agent is high in viable bacterium content, low in infectious microbe rate and stable in physicochemical property, meets the national standard requirements, and is non-toxic, harmless, low in preparation cost, green and environment-friendly. During application, the control effect is optimal when the composition is diluted by 400 times; when the composition is compounded with Zhongshengmycin according to the volume ratio of 3: 7 and copper hydroxide according to the volume ratio of 7: 3, the synergistic effect is remarkable, the kiwifruit bacterial canker can be effectively prevented and controlled, reduction and synergism of chemical pesticides are assisted, and technical support is provided for green and healthy development of the kiwifruit industry.

A new cherry tree particularly distinguished self-fertility, resistance to rain crack and resistance to bacterial canker, is disclosed.

The invention discloses a chemical preparation method and application of a 2-substituted-1-H-indole oxadiazole amine derivative, a series of compounds which take a urea or thiourea fragment as a linker and are substituted by different phenyl groups are synthesized on the basis of the structure of the 2-substituted-1-H-indole oxadiazole amine derivative, and the compounds have a good inhibition effect on pathogenic bacteria and can be used for preparing a novel compound for treating pathogenic bacteria. The compound has a good inhibition effect on pathogenic bacteria such as xanthomonas oryzae pv. Oryzae, citrus canker pathogen, kiwifruit bacterial canker pathogen and ralstonia solanacearum, and provides an important scientific basis for research, development and creation of new pesticides. The compound has a structure shown in the following general formula.

The application discloses a chromanone compound and application of the chromanone compound in prevention and treatment of bacterial canker disease of kiwi fruit, and a structural formula of the chromanone compound is shown in the following formula: wherein R is one or more of hydrogen, alkyl, alkenyl, alkynyl, nitro, alkoxy, cyano, amino, sulfonic acid group, halogenated alkyl, aryl, heteroaryl and halogen; and R' is alkyl or aryl. A synthesis method provided by the application uses cheap and easily obtained 2-hydroxyacetophenone as a basic raw material to perform conventional chemical synthesis, and the reaction can be completed under mild conditions without metal catalysts. The synthesis method has the advantages of simple reaction system, short reaction time, high yield, and the like, and can greenly synthesize the chromanone compound; meanwhile, the chromanone compound has obvious prevention and treatment effect on bacterial canker disease of kiwi fruit.

The application discloses a kind of Streptomyces for preventing and treating tomato bacterial disease, fermentation liquor and preparation method and application, and the Streptomyces for preventing and treating tomato bacterial disease is Streptomyces venezuelae FK-11, is preserved in China Microbial Culture Collection Center, the preservation number is CGMCC NO.25330, the preservation date is July 18, 2022, and the preservation address is No.3, Xili, Beichen, Beijing City, Chaoyang District.Compared with the prior art, the application provides a kind of soil antagonistic bacteria, which is a kind of Streptomyces that can produce chloramphenicol, isolated and selected from the surrounding of tomato root base, the fermentation liquor can effectively inhibit tomato bacterial disease, especially tomato bacterial canker disease and tomato bacterial wilt, it is derived from soil, and has strong environmental adaptability, and can effectively improve yield while inhibiting disease occurrence, the mode of action is fermentation liquor seed soaking, easy to operate, time-saving and labor-saving, environment-friendly, hope to replace chemical medicine, reduce chemical input, reduce environmental pollution, and guarantee the green and efficient production of tomato.

The invention provides a litsea cubeba extract-based composite agent for preventing and treating citrus ulcer diseases and a prevention and treatment method, and belongs to the technical field of citrus planting. The composite agent comprises 15-20 parts of a quaternized litsea cubeba extract, 5-8 parts of thiediazole copper, 0.5-1.5 parts of a wetting and dispersing agent and 40 parts of diatomite, and the quaternized litsea cubeba extract is obtained by mixing the litsea cubeba extract with triethylamine, dropwise adding alkyl bromide, and heating and refluxing the reaction mixture after dropwise adding. The litsea cubeba extract is endowed with strong targeted bactericidal ability through quaternization modification, the litsea cubeba extract is compounded with thiediazole copper to form dual mechanisms of membrane penetration and intracellular killing, the killing speed of citrus canker pathogens is increased, the high control effect is still kept while the use amount of copper is reduced, the development of drug resistance is remarkably delayed due to the multi-target effect, and the effect of killing the citrus canker pathogens is achieved. The design direction of green pesticides is met.

The invention belongs to the technical field of microbial engineering, and particularly relates to six compounds (1-6) extracted from a termite symbiotic bacterium Aspergillus scherzeri BYST01 liquid fermentation product, and a preparation method and application thereof. Specifically, the invention discloses six compounds, and the compound 6 is a new compound. According to the present invention, the Aspergillus scherzeri BYST01 metabolite has good antibacterial activity on three test pathogenic bacteria (Xanthomonas oryzae pv.oryzae, Xoo), Xanthomonas oryzae pv.oryzae, Xoc), and Pseudomonas syringae pv.actinidia, such that the Aspergillus scherzeri BYST01 metabolite has important value and potential for the development of the novel microbial source bactericide, and the application of the Aspergillus scherzeri BYST01 metabolite to the test pathogenic bacteria (Xanthomonas oryzae pv.oryzae, Xoc) and Pseudomonas syringae pv.actinidia,

The application belongs to the technical field of genetic engineering, and discloses an AcDRT100 protein and application thereof in improving disease resistance of kiwifruit. An AcDRT100 gene capable of improving the disease resistance of kiwifruit against bacterial canker disease is screened out, the gene is up-regulated in expression in kiwifruit of a'red sun' variety induced by Psa, the disease incidence of the kiwifruit is significantly reduced, and the plant resistance is improved. In addition, it is proved that the expression level of the disease resistance pathway genes AcNPR1, AcPR1, AcJAR1 and AcERF1 can be enhanced by transient overexpression of the AcDRT100 gene in kiwifruit, which provides theoretical support for the research on the molecular mechanism of the disease resistance of kiwifruit against bacterial canker disease. The application provides new insights and ideas for creating high-quality kiwifruit germplasm materials, reducing the dependence on pesticides in the prevention and treatment of bacterial canker disease of kiwifruit, and improving the quality and yield of kiwifruit.

This invention relates to the field of plant molecular genetic engineering, and more particularly to an encoded peptide that regulates resistance to kiwifruit bacterial canker and its applications. The encoded peptide is miPEP157a-2, which can be used to regulate the resistance of kiwifruit plants to kiwifruit bacterial canker. Spraying kiwifruit tissue culture seedlings with the encoded peptide solution, or culturing kiwifruit tissue culture seedlings with a medium containing the encoded peptide, can yield kiwifruit lines with enhanced resistance to kiwifruit bacterial canker. This application provides evidence supporting the participation of miPEP157a-2 and its encoded Ac-miR157a in regulating plant-pathogen interactions, and provides candidate genes for molecular breeding work to provide kiwifruit plants with high resistance to kiwifruit bacterial canker.

The invention discloses pseudomonas and application thereof, and belongs to the field of microorganism application, the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 28, 2025, the preservation number is CGMCC NO.36820, the strain belongs to Pseudomonas sp., and the name of the strain is pseudomonas T9. The invention further discloses a preparation method of the pseudomonas. The strain has a remarkable inhibition effect on plants infected with bacterial canker pathogens, and has important application potential in canker prevention and control.

The invention belongs to the technical field of medicinal chemistry, and particularly relates to a 3-position or 2-position substituted-1-H-indole triazolothiadiazole derivative compound as well as a preparation method and application thereof, a series of compounds are synthesized on the basis of a 3-position or 2-position substituted-1-H-indole triazolothiadiazole structure, and the 3-position or 2-position substituted-1-H-indole triazolothiadiazole derivative compound can be used for preparing a compound. The compound has a good inhibition effect on pathogenic bacteria and tumor cells, has a good inhibition effect on pathogenic bacteria such as Xanthomonas oryzae pv. Oryzae, Xanthomonas citri, Kiwi berry bacterial canker, ralstonia solanacearum and the like, and has a structural general formula shown in the specification.

A new cherry tree particularly distinguished yellow fruit with a kidney shape, precocious fruiting habit, self-fertility, extremely tolerant to rain crack and moderate resistance to bacterial canker, is disclosed.

The invention relates to an application of riboflavin in inhibiting pseudomonas syringae kiwifruit pathogenic variants in the field of microbial technology and plant disease control, which is used for overcoming the defects of chemical pesticides in the existing kiwifruit bacterial canker control technology. A new thought and a material basis are provided for developing an efficient, green and safe kiwifruit bacterial canker prevention and treatment medicament.