The invention discloses a
coprinus comatus strain for promoting seed
germination of
cremastra appendiculata and application of the
coprinus comatus strain, the strain is separated and screened from root tissues of
cremastra appendiculata, the strain is identified as
coprinus comatus through ITS (
internal transcribed spacer) sequencing, the Latin name is Coprinella micaceus, and the preservation number is CGMCC (China General
Microbiological Culture Collection Center) NO: 42051. The strain is inoculated into a
liquid culture medium for shake culture, and mycelia are collected and mixed with sterile
vermiculite and turfy soil to prepare a
fungicide; the method comprises the following steps: laying a sterilized culture medium formed by mixing
leaf mold,
perlite and
bark in a sterile culture container, spreading a
fungicide, spreading the treated
azalea seeds, covering the culture medium, and culturing by controlling the temperature, the illumination condition and the
relative humidity. According to the method, the
germination rate of the
rhododendron simsii seeds can reach 60%-70%, the
germination time is shortened to 15-20 days, the
survival rate of seedlings is high, operation is easy and convenient, cost is low, the method is suitable for large-scale breeding and
resource protection of the
rhododendron simsii, and a new thought is provided for research on symbiotic germination of orchid plants.