The invention discloses a soilless culture method of Chinese flowering cabbage. The method comprises the step of substrate preparation, wherein a seedling substrate and a culture substrate are included, wherein the seedling substrate is mainly prepared from, by weight, 15-20 parts of vermiculite, 10-15 parts of leaf mold, 5-8 parts of vermicompost, 5-8 parts of black tea powder and 3-5 parts of konjaku flour, and the culture substrate is mainly prepared from, by weight, 30-40 parts of vermiculite, 20-30 parts of rice bran, 20-30 parts of nuphar pumilum powder, 15-20 parts of sphaghnum powder, 10-15 parts of silkworm excrement, 10-15 parts of vermicompost, 10-15 parts of leaf mold, 8-10 parts of beta-cyclodextrin, 5-10 parts of konjaku flour, 5-10 parts of black tea powder, 5-10 parts of yeast, 6-8 parts of eggshell powder, 3-5 parts of nano zinc oxide, 3-5 parts of trehalose and 2-5 parts of lactic acid bacteria. The soilless culture method has the advantages of being high in yield, good in quality, good in economic benefit and the like.