46 results about "Botrytis" patented technology

The invention discloses a method for detecting drug resistance of botrytis cinerea to succinate dehydrogenase (SDHI) bactericides. On the basis of an RPA-CRISPR / Cas12a system, a specific primer pair amplification target fragment and crRNA for activating Cas12a trans-cleavage activity are designed aiming at key mutation sites related to drug resistance in a bactericide action target SdhB gene, so that fluorescent light visible to naked eyes can be generated only when a resistant strain sequence is matched with the crRNA, and the fluorescent light is opposite to the fluorescent light generated when a sensitive strain sequence is matched with the crRNA. The method provided by the invention overcomes the dependence on laboratories and large instruments in the traditional drug resistance detection method, provides key technical support for accurate prevention and control and drug resistance management of grape gray mold, and provides direct drug use basis for scientific prevention and control in the field.

The invention belongs to the technical field of microorganisms, and particularly relates to a pseudomonas TaRb6 capable of preventing diseases and promoting growth and application of the pseudomonas TaRb6. The TaRb6 strain provided by the invention belongs to a potential new species of pseudomonas, and the preservation number of the TaRb6 strain is CGMCC (China General Microbiological Culture Collection Center) No.36131. The strain TaRb6 and a fermentation inoculant prepared from the strain TaRb6 have good disease prevention and growth promotion functions on plants; the bacterial inhibition rate of sterile fermentation liquor of the strain TaRb6 on fusarium pseudograminearum reaches 46.54%-62.82%, and the bacterial colony diameter of the fusarium pseudograminearum can be reduced by 61.38% through metabolized volatile organic compounds of the strain TaRb6. After the TaRb6 bacterial suspension is used for seed soaking treatment of wheat, the incidence rate of wheat basal stem rot can be remarkably reduced, and the growth vigor of wheat can be promoted. In addition, the strain TaRb6 has broad-spectrum antibacterial ability, and the antibacterial rate of the strain TaRb6 on pathogenic bacteria of fusarium, botrytis cinerea, apical cyst and xanthomonas reaches 47.23%-67.02%.

The invention relates to the technical field of cut flower postharvest preservation, and discloses a preservation pre-treatment solution for maintaining moisture-nutrition-microecological balance in a storage period of babysbreath cut flowers and a synergistic application method thereof. The pre-treatment liquid is prepared from the following components in percentage by weight / volume: 15 to 30 g / L of cane sugar, 15 to 30 g / L of trehalose, 100 to 200 mg / L of 8-hydroxyquinoline citrate, 50 to 150 mg / L of citric acid, 50 to 100 mg / L of carboxymethyl chitosan, 0.3 to 1.0 g / L of lysozyme-chitosan compound, 5 to 15 mg / L of nano silver-loaded silicon dioxide, 0.1 to 1.0 g / L of manganese citrate and 0.5 to 1.0 g / L of calcium nitrate. And 5 to 10 mL / L of glycerol and 0.5 to 5.0 mL / L of Tween-20 in percentage by volume. Through component collaborative design-process precise control-package matching innovation, the bottleneck of double loss of water and nutrition in the storage period is broken through, the pain points of no flowering and gray mold outbreak after storage are solved, a whole-process nursing scheme is provided for long-distance and short-distance transportation and storage of the babysbreath cut flowers, the commodity rate of the cut flowers is remarkably increased, and the economic benefit is increased. The method is suitable for flower harvesting bases, cold chain transportation and wholesale markets, and is especially suitable for air transportation exits.

The application provides application of a bacillus subtilis K8 nano-seleniumized active bacterium agent in prevention and treatment of plant pathogenic bacteria, and belongs to the technical field of biological prevention and treatment of plant diseases. Bacillus subtilis The bacillus subtilis K8 nano-seleniumized active bacterium agent is obtained by fermenting and culturing bacillus subtilis K8 in a fermentation medium containing sodium selenite. Bacillus subtilis The bacillus subtilis K8 nano-seleniumized active bacterium agent has a strong prevention and treatment effect on cucumber fusarium wilt, cucumber botrytis blight, tomato early blight and tomato root rot, provides an efficient, safe and practical method for prevention and treatment of plant fungal diseases, has low cost, simple preparation process, is convenient for industrialization, and has good development value and market prospect.

The invention discloses a transcription factor AcWRKY84 gene for regulating and controlling gray mold resistance of kiwi fruits. After the expression of the gene is silenced, the gray mold resistance of kiwi fruits can be remarkably reduced. The AcWRKY84 gene is cloned, and the anti-gray mold effect of the AcWRKY84 gene in kiwi fruits is proved, specifically, after an experimental group is inoculated with viruses, compared with a control group, the disease symptoms of the fruits of the experimental group are aggravated, the infected area is obviously enlarged, and the enzyme activities of SOD, POD and CAT in the fruits are obviously reduced compared with the control group; sufficient gene resources can be provided for a kiwi fruit gray mold resistance gene pool, so that the possibility of successfully breeding high-gray mold-resistance varieties subsequently is greatly increased.

The invention provides an alkaloid Waltherione S analogue as well as a synthesis method and application thereof, and relates to the technical field of alkaloid Waltherione compounds. According to the present invention, the structures of the Waltherione A and the Antidesmone are simplified, the pyridine and pyridone alkaloid Waltherione S analogue is designed and synthesized, and the synthesized compound has broad-spectrum bacterial killing activity on banana wilt pathogen, wheat scab pathogen, banana anthracnose pathogen, phytophthora capsici pathogen, botrytis cinerea, rhizoctonia solani, rice blast pathogen, mango stem-end rot pathogen and the like, the compound also has good nematicidal activity on root-knot nematodes, provides a favorable candidate agent for natural, safe and low-toxicity innovative pesticides, and is expected to be applied to agricultural production.

The application discloses a kind of pyrimidine-substituted 1,2,4-triazole derivatives, it is characterized in that, with the structure expressed in general formula I or II:Wherein, R1 is hydrogen, methyl, ethyl, methoxy, ethoxy, thiomethyl, chlorine, fluorine, bromine, iodine, cyano, amine group, morpholine, triazole, pyrazole, trifluoromethyl, difluoromethyl, ethyl formate;R2 is hydrogen, methyl, ethyl, methoxy, chlorine, fluorine, bromine, iodine, cyano, amine group, trifluoromethyl, ethyl formate;R3 is hydrogen, methyl, ethyl, methoxy, ethoxy, thiomethyl, chlorine, fluorine, bromine, cyano, amine group, morpholine, triazole, pyrazole, trifluoromethyl, difluoromethyl.The compound of the application shows excellent activity against plant pathogens, such as rice sheath blight fungus, cucumber botrytis, blueberry botrytis, strawberry botrytis, tobacco botrytis, and sclerotinia sclerotiorum.

The application of an endogenous yeast and its application in resisting apple botrytis blight belongs to the field of microorganism technology. In order to solve the technical problems that apple is easily infected by botrytis cinerea to cause botrytis blight during transportation and storage, and the adverse effects of chemical fungicides on environment and human health, the application screens an endogenous yeast from apple, which is identified as Bullera alba through morphological and molecular biological analysis. The experiment proves that the cell suspension of the strain PGY-2 has a control effect on postharvest botrytis blight of apple, and can well colonize at the apple wound, can antagonize the botrytis blight pathogen by forming a biofilm and inducing the activity of various defense enzymes, and the strain PGY-2 can be used for preparing a fungicide for preventing and treating postharvest botrytis blight of apple.

The invention discloses an application of a strawberry transcription factor FaWRKY21 in gray mold resistance after strawberry harvesting, and is characterized in that a nucleotide sequence of a gray mold resistance related gene FaWRRKY21 after strawberry harvesting is as shown in SEQ ID NO.1, and an amino acid sequence is as shown in SEQ ID NO.2. The invention further provides a method for improving gray mold resistance of strawberries after strawberry harvesting, and the method is realized by improving the expression quantity of the FaWRKY21 gene in strawberry cells. Specifically, a recombinant vector containing the strawberry transcription factor FaWRKY21 coding gene is transferred into strawberries through agrobacterium, and transgenic strawberry fruits overexpressed by the strawberry transcription factor FaWRKY21 coding gene are constructed and have the advantages that disease-resistant defense response of the strawberries can be remarkably activated, the morbidity is effectively inhibited, the scab diameter is reduced, and the disease resistance of the strawberries is improved. Therefore, the resistance of strawberries to the postharvest gray mold is improved.

A transcription factor for regulating resistance of kiwifruit to botrytis blight AcWRKY84 The application relates to a gene, and after the expression of the gene is silenced, the resistance of kiwifruit to botrytis blight is significantly reduced. AcWRKY84 The application clones the gene and proves the role of the gene in resisting botrytis blight in kiwifruit, and specifically, after the experimental group is inoculated with the virus, the disease symptoms of the experimental group are more serious, the infected area is obviously increased, and the SOD, POD and CAT enzyme activities in the fruit are significantly reduced compared with those of the control group, so that sufficient gene resources can be provided for a botrytis blight resistance gene library, and the possibility of realizing successful breeding of a high botrytis blight resistance variety is greatly increased.

The present application provides a carvacrol tertiary amine derivative, a preparation method and an application in preparing a plant source fungicide, the carvacrol tertiary amine derivative contains a cyclic secondary amine substituent, the preparation method of the derivative is that an intermediate II is dissolved in ethanol, then a cyclic secondary amine is added, heating and stirring are carried out, reflux is kept, after TLC detection reaction is completed, ethanol is removed under reduced pressure, then extraction, water washing and purification are carried out, and the carvacrol tertiary amine derivative is prepared. The carvacrol tertiary amine derivative prepared by the present application exhibits obvious bacteriostatic activity on cucumber anthracnose fungus, tomato botrytis blight fungus, wheat scab fungus and wheat take-all fungus. The preliminary bacteriostatic activity of carvacrol tertiary amine derivatives 1, 3, 10 and 14 on wheat take-all fungus is 60.8%-83.2%, which is significantly better than that of positive control hymexazol (48.6%) and reference control carvacrol (45.0%).

The application discloses application of RcARF8 genes in enhancing resistance of Chinese rose to botrytis blight, and a nucleotide sequence of the RcARF8 gene is shown as SEQ ID NO. 1. In the application, the RcARF8 gene is silenced and overexpressed in petals of Chinese rose in a good growth state and free from infection of botrytis blight, and it is found that petals treated by silencing the RcARF8 gene have a larger disease spot area and a reduced resistance to botrytis blight, and petals treated by overexpressing the RcARF8 gene have enhanced resistance to botrytis blight.

The invention belongs to the technical field of microorganisms, and particularly relates to botrytis isoschiff bud yeast with potassium hyperaccumulation capacity and application of the botrytis isoschiff bud yeast. Aiming at the problem that a strain capable of enriching potassium ions in wastewater is lacked in the prior art, the invention provides the botrytis isoschizochytrium JK-1 which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on January 12, 2026, and the preservation number of the botrytis isoschizochytrium JK-1 is CGMCC (China General Microbiological Culture Collection Center) No.39121. The invention further provides a preparation method of the botrytis isoschizochytrium JK-1. The strain JK-1 is found for the first time, has the characteristic of potassium hyperaccumulation, not only can purify biogas slurry, but also can be used as a biological pump to concentrate and recover potassium resources.

This invention relates to the field of agricultural biotechnology, and in particular to a stress-resistant, antifreeze, and preservative biofilm formulation and its preparation method. The stress-resistant, antifreeze, and preservative biofilm formulation is composed of the following raw materials in parts by weight: 13-28 parts film-forming matrix, 5.5-11.5 parts antifreeze active component, 2.51-6.05 parts stress-resistant active component, 7-14 parts crosslinking and plasticizer, 0.1-0.5 parts natural antibacterial agent, and 55-75 parts deionized water. This invention innovatively achieves a four-fold synergy of physical barrier, active stress resistance, efficient antifreeze, and natural antibacterial properties. The film-forming matrix forms a dense and breathable physical barrier, inhibiting moisture loss; the stress-resistant components construct an active stress-resistant system, enhancing the stress resistance of agricultural products; the antifreeze components achieve efficient antifreeze by inhibiting ice crystal formation and stabilizing cell membrane structure; and the natural antibacterial agent specifically inhibits postharvest pathogens such as Penicillium and Botrytis. The four components work synergistically to comprehensively solve multiple problems during the storage of agricultural products.

The present invention relates to a microbial strain having superior fungicidal and / or nematicidal activity, as well as its progeny and derivatives, compositions comprising the same, and methods of using the same, inter alia in agriculture. The strain, as well as its progeny and derivatives, is capable of increased fungicidal activity against at least one fungi selected from the group consisting of: Botrytis, Fusarium, and Rhizoctonia when compared to Bacillus velezensis strain GB03.

The invention relates to the field of pest prevention and control, and particularly discloses an essential oil composition and method for preventing and controlling book lice bostrychus, active ingredients of the essential oil composition comprise thyme essential oil and lavender essential oil, and the volume ratio of the thyme essential oil to the lavender essential oil is (8-10): 1. The method for preventing and controlling the book lice botrytis comprises the step of treating the breeding environment of the book lice botrytis botrytis by using the oil composition in a fumigation manner. The composition provided by the invention has a remarkable synergistic interaction effect on the bostrychus bostrychus, especially adults of the bostrychus bostrychus, and is environment-friendly, and the dosage can be reduced.

This invention relates to the application of flunilic acid in the control of fungal diseases in crops. This invention discovers that flunilic acid can inhibit the growth of crop pathogens, thus demonstrating that flunilic acid can be used as a pesticide to control and inhibit crop pathogens, particularly those in the Ascomycota phylum (…). Ascomycota Pathogenic fungi in ); for example, it could be the genus *Gastropoda* ( Magnaporthe ), Cyclospora spp. ( Cochliobolus Fusarium ( ) Fusarium ), Botrytis ( Botrytis ) and Sclerotinia spp. ( Sclerotinia Pathogenic fungi in ).

The invention discloses an inorganic plastic oxygen composite material and application thereof in a green agriculture and plant maintenance system. The inorganic plastic oxygen composite material is prepared from the following components: an inorganic internal peroxide bridge composite compound, an inorganic mediated catalytic material and a material balancing and stabilizing system, through the synergistic effect of all the components, the generation amount and the utilization efficiency of singlet oxygen are remarkably improved, and then the excellent pest control and pesticide residue degradation effects are achieved. In actual agricultural application, an aqueous solution prepared from the composite material can generate air containing singlet oxygen after being atomized and aerated, the air is uniformly released through a greenhouse pipeline, germ spores in the air can be quickly killed, and meanwhile, the composite material is sprayed on the surfaces of crops, so that mold and gray mold of the crops such as tomatoes and cucumbers can be effectively prevented and treated; the efficient degradation effect on organic phosphorus pesticide residues on the surfaces of the vegetables is realized. The whole technical scheme does not need to use chemical pesticides, meets the development requirements of green agriculture, can effectively reduce pesticide residue pollution, and protects the ecological environment of soil.

The invention discloses a bactericidal composition for preventing and treating gray mold and application thereof, and belongs to the technical field of pesticides. The effective components of the bactericidal composition disclosed by the invention are the flurobixafen and the zhongshengmycin, wherein the mass ratio of the flurobixafen to the zhongshengmycin is (2-9): 1. The Zhongshengmycin and the chlorine fluorine bixafen are compounded for preventing and treating the gray mold, so that the pesticide dosage is reduced during use, the prevention and treatment cost is reduced, the generation of pathogenic bacterium resistance is delayed, and the pollution to the environment is reduced.

The invention belongs to the technical field of medicine innovation, and particularly relates to a 5-substituted pyrimido pyrazolyl oxazoline compound as well as a preparation method and application thereof. The 5-substituted pyrimido pyrazolyl oxazoline compound provided by the invention has a structure as shown in a formula 1, and comprises substituted pyrimido pyrazole and substituted oxazoline substructures, the compound has a remarkable inhibition effect on various plant pathogenic fungi (such as pyricularia oryzae, colletotrichum capsici, rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, phytophthora capsici and the like), and is expected to be used as a novel bactericide candidate compound or directly used as a bactericide. Formula 1.

The present application relates to the technical field of pesticide compound, especially to a kind of fungicidal composition containing boscalid and fludioxonil and application thereof;A kind of fungicidal composition containing boscalid and fludioxonil, the active ingredient contained in the fungicidal composition is boscalid and fludioxonil;The mass ratio of the boscalid and fludioxonil is 10:20~29:1;The present application discloses a kind of fungicidal composition containing boscalid and fludioxonil and application thereof, the boscalid and fludioxonil added within a certain ratio range show good synergistic effect to tomato botrytis blight, the fungicidal effect of the combination of the two is obviously higher than single agent, and the co-toxicity coefficient is above 120, the fungicidal composition prepared not only reduces its use dose and cost, but also reduces pesticide residue, reduces the harm to environment.

The invention relates to the technical field of agriculture, and particularly discloses a comprehensive pest and disease damage prevention and control method combining winter peach fruit bagging with trapping and killing technology, which comprises the following steps: in a winter peach physiological fruit dropping period, suspending insect sex pheromone trappers in an orchard, and monitoring the adult sexual period of peach fruit moths and oriental fruit moths; s2, according to the monitoring result of the step S1, all fruits are uniformly bagged before the initial emergence period of the target pest adults and after fruit setting is completed; physical bagging and various trapping and killing technologies are systematically integrated in time sequence and space, pest situation monitoring is used as a guide, the optimal window period of bagging is determined, physical isolation measures are made to play the maximum effect, a composite trapping and killing system is deployed subsequently, space differentiation configuration is conducted according to ecological habits and activity rules of different pests, and the pest trapping and killing effect is improved. Accurate coverage of the trapping and killing range and the target object is achieved.

The application of leidinmycin compounds in inhibiting plant pathogenic fungi and preventing and treating plant diseases belongs to the technical field of pesticide fungicides. In order to screen leidinmycin compounds capable of being used for preventing and treating plant diseases caused by plant pathogenic fungi or plant pathogenic oomycetes, five kinds of leidinmycin compounds are used for inhibiting plant pathogenic fungi and preventing and treating plant diseases. The leidinmycin compounds have strong inhibitory effect on plant pathogenic fungi, including the growth of fungal hyphae in Sclerotinia, Rhizoctonia, Elsinoe, Botrytis and Alternaria and the growth of oomycete hyphae in Pythium, Peronospora and Pseudomonas. In addition, the leidinmycin compounds have good prevention and treatment effect on plant diseases caused by the above plant pathogenic fungi and plant pathogenic oomycetes, and have no toxic side effect on plants, and can be developed and applied as effective components of pesticides or plant pathogenic fungicides.

The invention discloses streptomyces virginiae KH13 as well as a fungicide and application thereof, and particularly relates to the technical field of agricultural microorganisms. The streptomyces virginiae KH13 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the classification name of the streptomyces virginiae KH13 is Streptomyces virginiae KH13, the preservation date is January 13, 2026, and the preservation number is CGMCC NO.37376. The streptomyces virginiae KH13 disclosed by the invention has the advantages that the streptomyces virginiae KH13 can be used for preparing the streptomyces virginiae KH13; the strain has the physiological and biochemical characteristics of nitrogen fixation, starch degradation, cellulose degradation, hydroxyl type siderophore generation, ammonia production and the like. The invention also provides a microbial inoculum containing the strain and application of the microbial inoculum in inhibiting peach pathogenic bacteria and relieving continuous cropping obstacles of peach trees. The strain can regulate and control a soil microbial community structure, inhibit peach tree continuous cropping obstacle related pathogenic bacteria such as red shell bacteria, fusarium, botryosphaeria and the like, and promote proliferation of beneficial microorganisms of streptomyces in soil. The invention provides a new resource and approach for green prevention and control of peach pathogenic bacteria and sustainable development of peach industry, and has a relatively high industrial application prospect.

The invention relates to the technical field of prevention and treatment of greenhouse tomato gray mold, in particular to a biocontrol preparation for greenhouse tomato gray mold and a preparation method of the biocontrol preparation. The biocontrol preparation is prepared from the following components in parts by weight: 30 to 40 parts of biocontrol microorganisms, 50 to 60 parts of traditional Chinese medicine extract and 10 to 15 parts of plant prebiotics or plant rhizosphere prebiotics, the biocontrol microorganism is composed of streptomyces albidoflavus WP-green 1 and bacillus amyloliquefaciens; the traditional Chinese medicine extract is prepared by compounding a scutellaria baicalensis extract and a wormwood extract; the preparation method comprises the following steps: step 1, preparing a composite biocontrol microbial agent; 2, preparing a traditional Chinese medicine extract; step 3, preparing plant prebiotics / plant rhizosphere prebiotics; step 4, preparing a biocontrol agent; the target bacteriostasis of antagonistic microorganisms, broad-spectrum bacteriostasis and plant resistance induction of traditional Chinese medicine components, promotion of biocontrol microorganism growth by plant prebiotics, improvement of the survival rate, prolonging of the shelf life of products and promotion of biocontrol bacterium rhizosphere colonization are combined, and the purposes of efficiently defending and preventing and treating greenhouse tomato gray mold are achieved through the synergistic effect of the antagonistic microorganisms, the broad-spectrum bacteriostasis of the traditional Chinese medicine components, the plant prebiotics, the biocontrol microorganisms and the plant prebiotics.

The invention relates to a tomato-source antibacterial protein, application of the tomato-source antibacterial protein to prevention and treatment of tomato gray mold and a biological preparation for preventing and treating the tomato gray mold, and belongs to the technical field of tomato disease prevention and treatment. In order to solve the problem that a tomato-derived antibacterial protein for preventing and treating tomato gray mold is lacked in the prior art, the invention provides a tomato ABS258 antibacterial protein and a tomato ABS121 antibacterial protein, and the amino acid sequence of the tomato ABS121 antibacterial protein is from the first site to the 121th site of the amino acid sequence of the tomato ABS258 antibacterial protein. The specific botrytis cinerea resistant protein provided by the invention can effectively inhibit the growth of botrytis cinerea hyphae at low concentration and prevent pathogenic bacteria from infecting tomato leaves, and has the natural advantages of clear gene source and high compatibility with tomatoes, and the differentiated protein structure provides flexible choices for pesticide development aiming at different application scenes.

The present application relates to the field of microbial technology, a kind of biocontrol agent for preventing and treating tomato botrytis, biocontrol agent and its application.The biocontrol agent is Bacillus atrophaeus HLTL04 strain, preserved in China General Microbiological Culture Collection Center on June 20, 2023, and the preservation number is CGMCC NO.27658.The biocontrol agent of the present application has a strong inhibitory effect on tomato botrytis, and the HLTL04 strain can effectively inhibit the development of tomato rot caused by botrytis.The HLTL04 has a good growth-promoting effect on tomato plants.The HLTL04 strain can successfully colonize in tomato fruits.The Rif-resistant HLTL04 can colonize in tomato stems, leaves and roots, and shows a dynamic change trend of more at first and less later, gradually decreasing.The colonization amount in tomato rhizosphere soil gradually decreases, and the colonization amount in each part is different, with relatively large increase in roots compared to stems and leaves, indicating that HLTL04 can colonize and parasitize in tomato roots, stems and leaves for a long time, and the root surface of tomato plants is more suitable for its growth and reproduction than other parts.

The application discloses a pesticide application method based on a grapevine downy mildew prediction model, relates to the technical field of agricultural plant protection, and mainly aims to provide a pesticide application method based on a grapevine downy mildew prediction model, which can dynamically simulate a complete disease epidemic process from overwintering inoculum release to secondary cycle establishment. The application provides a pesticide application method based on a grapevine downy mildew prediction model, which directly outputs an infection risk early warning through the prediction model, helps to reduce the use frequency and dosage of pesticides, reduces the cost and environmental risk, and meanwhile, can be calibrated locally according to different regions or varieties. The construction framework can also provide a reference for the development of other air-borne and rain-borne fungal disease prediction models, and then a mechanism model capable of dynamically simulating a complete disease epidemic process from overwintering inoculum release to secondary cycle establishment is constructed, so that the technical effect of improving the prediction accuracy is achieved.

The invention discloses application of Yarrowia guilliermondii JY19 in prevention and treatment of gray mold of ginseng, and relates to the field of biological prevention and treatment. According to the invention, the Yarrowia guilliermondii JY19 is separated and screened from ginseng under forest, and the strain shows a remarkable antagonistic effect on botrytis cinerea of ginseng, can remarkably inhibit the growth of botrytis cinerea and the formation of a hypha structure, effectively reduces the occurrence rate of gray mold of medicinal plants such as ginseng and the like, and has a good application prospect. The resistance of ginseng and other medicinal plants to gray mold is obviously enhanced.

To provide an oligonucleotide enabling accurate grasp of the initial onset of Welsh onion white rot and a method for detecting both group A and group B strains of Sclerotium cepivorum.SOLUTION: The detection method according to the present invention uses an oligonucleotide of 15-30 base-length that is capable of annealing to any one region, or a complementary region thereof, selected from gene regions of glyceraldehyde-3-phosphate dehydrogenase (G3PDH), heat shock protein 60 (HSP60), and calmodulin (CaM) of plant-pathogenic Ascomycota, the region being conserved in both group A and group B strains of Sclerotium cepivorum and exhibiting polymorphisms in Botrytis, Dumontinia, Macrophomina, Sclerotinia, and Sclerotium., Sclerotinia, and Sclerotium.SELECTED DRAWING: Figure 7