The invention discloses a biocontrol bacterium and trifloxystrobin composition, and belongs to the technical field of bactericides. The composition contains the following active components: Bacillus subtilis and trifloxystrobin. The composition can effectively control rice sheath blight, rice false smut, rice blast, seedling blight, tomato late blight, tomato botrytis, wheat powdery mildew, wheat sheath blight, cotton Rhizoctonia solani Kuhn, cotton fusarium wilt, cotton verticillium wilt, soybean root rot, corn sheath blight, cucumber botrytis, cucumber wilt, tobacco brown spot, tobacco bacterial wilt disease, various field and garden crop root rot, seedling blight and other various diseases, and substantially reduces the pesticide cost, so the pesticide cost is reduced, the control range is enlarged, environmental pollution is reduced, and the pesticide harm and the pesticide resistance risk are reduced.