The present invention discloses main components, formulations, statuses and clinical uses of a medical composition containing fish serine protease and an antibacterial active compound. The medical composition comprises fish serine protease, an antimicrobial active compound, a medically-acceptable excipient and water for injection, can be prepared into an emulsion, a spraying agent, a liniment, a drop and a gargarisma, has statuses such as a liquid, an emulsion, a paste, a gel, liposome particles, a nanometer gel and microcapsules, and can be used for burning and other skin trauma repairs, bacteriostasis and bacterial killing, eczema, dermatitis, psoriasis vulgaris and other skin infections, bone joint inflammations, and systemic disease prevention and treatments in clinic. According to the present invention, the fish serine protease and the antibacterial compound are combined, wherein the composition retains the fish serine protease activity and contains the bacterial killing bacteriostasis compound, such that the composition has a strong bacterial killing effect, and provides good prevention and treatment effects for a plurality of diseases.