5 results about "Respiratory burst" patented technology

A method for estimating a number of phagocytes in a body fluid of an animal includes stimulating NADPH oxidase activity of the phagocytes; and quantifying a resulting reductive dioxygenation of a chemiluminigenic substrate by an emitted chemiluminescence of the chemiluminigenic substrate using an instrument capable of measuring light. The NADPH oxidase activity of the phagocytes is preferably stimulated by an immunologic or chemical capable of activating a respiratory burst by the phagocytes. The NADPH oxidase activity of the phagocytes is preferably stimulated by a stimulus in solution or coated to a surface contacted by the phagocytes. The stimulus is preferably phorbol myristate acetate (PMA). The animal is preferably human, and the body fluid is preferably blood and / or spinal fluid. The phagocytes are preferably neutrophil leukocytes, and the chemiluminigenic substrate is preferably lucigenin (N,N′-dimethyl-9,9′-biacridinium dinitrate). Also provided is a method of treatment based on the estimation method.

The invention discloses a respiratory event recognition method and device, a storage medium and a computer program product, and relates to the technical field of medical monitoring, the method comprises the following steps: collecting throat vibration data through a three-axis acceleration sensor of throat snore-ceasing equipment, and generating a vibration data sequence according to the throat vibration data; calculating an environmental noise baseline according to the vibration data sequence, and identifying a breathing emergency based on the environmental noise baseline; and calculating the current breathing amplitude according to the vibration data sequence, and calculating a breathing amplitude ratio based on the current breathing amplitude and a pre-constructed breathing cycle amplitude model so as to identify an apnea event. The throat vibration data is collected through the three-axis acceleration sensor, and the synergistic effect of the environmental noise baseline and the respiratory cycle amplitude model is combined, so that the recognition precision and recognition efficiency of the apnea event and the respiratory emergency are improved.

A chemiluminescence-based method for quantifying the number of phagocytes in a body fluid of an animal includes stimulating NADPH oxidase activity of the phagocytes; and quantifying a resulting reductive dioxygenation of a chemiluminescent substrate by an emitted chemiluminescence of the chemiluminescent substrate using an instrument capable of measuring light. The NADPH oxidase activity of the phagocytes is preferably stimulated by an immunologic or chemical capable of activating a respiratory burst by the phagocytes. The phagocytes are preferably neutrophil leukocytes, and the chemiluminescent substrate is preferably lucigenin (N, N′-dimethyl-9,9′-biacridinium dinitrate). Also provided is a method of measuring myeloperoxidase activity of the neutrophils present in a sample of blood or body fluid based on the non-reductive (simple) dioxygenation of a chemiluminescent substrate, preferably luminol (5-amino-2,3-dihydro-1,4-phthalazinedione). The methods use dual chemiluminescent probes on microvolumes of blood or body fluid to enable reliable optical measurements applicable to point-of-care testing.

This invention relates to a fish interferon regulator IRF4 gene, protein, expression vector, and their applications, belonging to the field of fish molecular immunology technology. The fish interferon regulator IRF4 is derived from the oval pomfret, and the cDNA nucleotide sequence of the oval pomfret IRF4 gene is shown in SEQ ID NO.1. The amino acid sequence of the protein encoded by the oval pomfret IRF4 gene is shown in SEQ ID NO.2. This invention also provides an expression vector containing the oval pomfret IRF4 gene, and an anti-Vibrio harveyi infection preparation containing the protein or a eukaryotic expression vector. The oval pomfret IRF4 protein of this invention can promote the respiratory burst activity and phagocytic capacity of oval pomfret head kidney macrophages. Overexpression in oval pomfret... Believe After IRF4 is activated, the body's antibacterial ability can be significantly improved, and it can be used as an immune enhancer.

A method for estimating a number of phagocytes in a body fluid of an animal includes stimulating NADPH oxidase activity of the phagocytes; and quantifying a resulting reductive dioxygenation of a chemiluminigenic substrate by an emitted chemiluminescence of the chemiluminigenic substrate using an instrument capable of measuring light. The NADPH oxidase activity of the phagocytes is preferably stimulated by an immunologic or chemical capable of activating a respiratory burst by the phagocytes. The NADPH oxidase activity of the phagocytes is preferably stimulated by a stimulus in solution or coated to a surface contacted by the phagocytes. The stimulus is preferably phorbol myristate acetate (PMA). The animal is preferably human, and the body fluid is preferably blood and / or spinal fluid. The phagocytes are preferably neutrophil leukocytes, and the chemiluminigenic substrate is preferably lucigenin (N,N′-dimethyl-9,9′-biacridinium dinitrate). Also provided is a method of treatment based on the estimation method.