The invention discloses a polarization calibration method for an antenna system with an automatic polarization adjustment function, and relates to a polarization calibration method in the field of communication. The antenna system with the automatic polarization adjustment function comprises a horizontally polarized antenna, a vertically polarized antenna, an automatic polarization adjustment module and a polarization control circuit, wherein the automatic polarization adjustment module consists of a first amplifier, a second amplifier, match circuits of the first amplifier and the second amplifier, a first numerical control phase shifter, a second numerical control phase shifter, a first numerical control attenuator, a second numerical control attenuator and a combiner/shunt; the automatic polarization adjustment function is completed by automatically adjusting an amplitude ratio of an orthogonal double-line polarization unit; and the polarization calibration process is completed by an automatic calibration method. The method has the advantages that the adjustment speed is high, the adjustment from time to time can be realized, the adjustment effect is good, and antenna outlines are not changed. The method is suitable for manufacturing the antennas with the polarization adjustment functions in satellite communication or other communication systems, in particular to low-outline satellite communication antennas of mobile carriers.