21 results about "Foxtail" patented technology

The invention discloses a non-tissue-culture-dependent pearl millet injection transformation method, and belongs to the technical field of plant genetic engineering. The method comprises the following steps: selecting germinated seeds of pearl foxtail millet with the primary bud length of 0.8-1.0 cm as receptors; preparing agrobacterium liquid, and adjusting the concentration to OD600 = 0.5; injecting the bacterial liquid into the embryo along the coleoptile direction of the seed until the bacterial liquid seeps from the end part of the coleoptile; co-culturing the transformed receptor for 24 hours under a dark condition after injection, and transplanting the transformed receptor into a seedling culture pot for cultivation; and finally, carrying out PCR (Polymerase Chain Reaction) screening. By optimizing key parameters such as the receptor development stage, the agrobacterium strain type and the bacterial liquid concentration, efficient genetic transformation of the pearl millet without tissue culture is achieved, and the T0 transformation positive rate reaches 63%. According to the invention, the transformation process is simplified, the transformation period is shortened, the cost is reduced, the transformation efficiency and stability are improved, and an effective technical support is provided for gene function research and molecular breeding of the pearl millet.

The utility model discloses a foxtail millet seeding row spacing adjusting device, which comprises an installation rod, a plurality of groups of seeding assemblies are arranged on the installation rod and are installed on the installation rod through an adjusting mechanism, the adjusting mechanism comprises a plurality of groups of adjusting holes, an adjusting sleeve, an inserting rod and a quick fixing mechanism, the adjusting holes are distributed on the installation rod, and the inserting rod is sleeved on the adjusting sleeve. The adjusting sleeve is connected with the multiple sets of seeding mechanisms and slides on the outer wall of the mounting rod, the inserting rod is inserted into the adjusting hole, the quick fixing mechanism comprises a fixing sleeve, clamping pieces, clamping grooves, compression springs, a push plate and a quick detaching mechanism, the fixing sleeve is arranged at the top end of the inserting rod, the multiple sets of clamping pieces are distributed on the inner wall of the fixing sleeve, and the multiple sets of clamping grooves are distributed in the outer wall of the inserting rod; a clamping structure of a fixing sleeve, a clamping piece and a clamping groove in the quick fixing mechanism is adopted, the elastic pressing design of a compression spring and a push plate is combined, and quick fixing of the adjusting position is achieved through the synergistic effect of multiple sets of components.

The application provides application of a CAPS molecular marker GW18 in identification of foxtail millet (Setaria italica) resistance to rice blast, and belongs to the technical field of molecular marker breeding. The application provides the application of the CAPS molecular marker GW18 in identification of the foxtail millet resistance to the rice blast, and G / T polymorphism exists at 34523782 bp of the 6th chromosome of the foxtail millet; when the polymorphism is G, the resistance to the rice blast cannot be recognized by a restriction enzyme, and is identified as the susceptibility to the rice blast; when the polymorphism site is T, the GW18 target sequence can be recognized by the restriction enzyme and is cut into two segments, and is identified as the resistance to the rice blast. The marker has a high co-separation rate with the disease resistance, a rapid, accurate and low-cost detection method, and can be used for rapid identification of foxtail millet germplasm resources resistant to the rice blast and molecular marker assisted breeding, and has important significance for improving breeding efficiency and guaranteeing safe production of the foxtail millet.

This invention discloses a photoperiod regulation gene of foxtail grass. SvEhd1 Its application in shortening the research cycle of rice resistance to rice blast falls under the fields of genetic engineering and plant breeding. SvEhd1 The gene nucleotide sequence is shown in SEQ ID NO.1. This invention utilizes cloning... SvEhd1 Genes and their promoters were used to construct recombinant expression vectors, which were then transformed into the blast-susceptible rice variety "Taipei 309," resulting in transgenic rice materials with significantly shortened growth periods. Under natural and artificial long-day conditions, the heading time of the transgenic plants was significantly earlier than that of the wild type, the generation rate was accelerated, and the blast susceptibility of the original variety was not altered. The transgenic materials and methods provided by this invention can effectively shorten the experimental cycle for verifying the function of rice blast resistance genes and evaluating resistance, providing an efficient experimental system for rice disease resistance research.

The application discloses SiERF4 and SiERF4-like The application of the genes in improving the salt tolerance of millet belongs to the technical field of crop genetic breeding. SiERF4 The genes and SiERF4- like Single knockout or double knockout is performed on the genes to obtain a mutant strain of millet with significantly improved salt tolerance. SiERF4 The mRNA sequence coded by the gene is shown as SEQ ID NO. 1, and the amino acid sequence is shown as SEQ ID NO. 2; SiERF4-like The mRNA sequence coded by the gene is shown as SEQ ID NO. 3, and the amino acid sequence is shown as SEQ ID NO. 4. The application first finds that SiERF4 and SiERF4- like The gene is a negative regulation gene responding to salt stress, and knocking out the gene can significantly improve the salt tolerance of the millet, and has important practical significance for cultivating salt-tolerant millet varieties and developing and utilizing saline land.

This invention relates to the fields of compound synthesis technology and pesticide technology, specifically to natural naphthoquinone derivatives and their preparation methods and applications. The natural naphthoquinone derivatives are either natural naphthoquinone derivatives containing aryl carboxylic acids or natural naphthoquinone derivatives containing diphenyl ether units. Some of the natural naphthoquinone aryl carboxylic acid derivatives and natural naphthoquinone diphenyl ether derivatives of this invention exhibit 100% control efficacy against six weeds (barnyard grass, crabgrass, foxtail grass, velvetleaf, amaranth, purslane, etc.) at a dosage of 300 a.g. / ha. These compounds achieve good weed control effects at low dosages and have relatively good safety for crops such as corn, wheat, peanuts, soybeans, and cotton. They can be used as selective herbicides in agriculture. The compounds of this invention can be developed and used as potential PSII inhibitors and post-emergence herbicides for broadleaf weeds. The derivatives have simple structures and preparation processes, low production costs, and broad application prospects.

The invention provides a breeding method of a drought-resistant and high-temperature-resistant millet variety. The breeding method comprises the steps of parent selection, cross pollination, F planting and screening, F and offspring planting and multi-generation screening, stable strain identification and the like. According to the method, parents with obvious drought resistance and high temperature resistance are selected for hybridization, drought stress and high temperature treatment are set in different growth stages, quality screening is combined, and the millet strain with stable characters is bred in multiple generations. The method can accurately and directionally select, accelerates character stability and shortens the breeding period. The bred millet variety has the characteristics of drought resistance and high temperature resistance, the yield reduction range is small under the high-temperature drought condition, and the quality is good. According to the method, the breeding efficiency is improved, the grain yield and quality are guaranteed, agricultural sustainable development is promoted, and the requirement of the market for the high-quality stress-resistant millet variety is met.

The invention provides non-fried broom corn millet crisp noodles and a preparation method thereof. The preparation method comprises the following steps: 1, kneading dough: uniformly mixing wheat flour, broom corn millet flour and vital gluten in a dry powder state, pouring the mixture into a dough kneading machine, and adding salt and distilled water; step 2, dough fermentation: putting the dough wadding obtained in the step 1 into a fermentation box for fermentation; step 3, pressing dough and cutting into strips: firstly performing composite rolling on the dough batting to prepare dough sheets, then performing continuous rolling on the dough sheets to obtain smooth and flat dough sheets with uniform thickness, and then cutting the dough sheets into strips; step 4, steaming: uniformly placing the product obtained in the step 3 in a steaming plate, and placing the steaming plate in a noodle steaming machine for steaming; and step five, performing hot air drying: putting the steamed noodles into a mold, and putting the mold into an air flow type dryer for hot air drying to obtain the non-fried broom corn millet crisp noodles. The crisp noodles prepared by the invention are added with special coarse cereal broom corn millet powder, and are dried by non-frying hot air, so that the crisp noodles are low in fat content and crisp in mouth feel, and have outstanding broom corn millet fragrance.

This invention discloses a compound with a (9β-H)-pinene core that inhibits weed growth, its preparation method, and its applications. In this invention, the ethanol extract of the tuberous part of *Cornus mansoni* is purified by macroporous resin column chromatography, followed by recrystallization and C244-distillation. 18 Gradient elution was performed using reversed-phase medium-pressure column chromatography; finally, its chemical structure was identified by methods such as proton NMR, carbon NMR, and comparison with literature data. The compound icacenone in this invention exhibits significant inhibitory effects on the root growth of Arabidopsis thaliana, barnyard grass, and foxtail grass, while remaining safe for the growth of rice and Job's tears, making it a potential pesticide formulation for developing biological herbicides.

A crystal of L-glufosinate ammonium salt, a preparation method therefor and an application thereof, the crystal of L-glufosinate ammonium salt comprising two novel crystal forms, namely novel crystal form D and crystal form E. On the basis of these two crystal forms having low hygroscopicity, the crystal forms not only have excellent stability, but also have good herbicidal effects, having a plant control effect on weeds such as sorrel, orange foxtail, sun spurge, bluegrass and other weeds can reach more than 85%.

This application discloses a multi-source grain and bean compound health-preserving cereal formula, which is composed of the following raw materials in the following weight proportions: 2-8 coweye beans, 3-9 kidney beans, 5-9 red beans, 2-7 white kidney beans, 5-8 corn kernels, 4-8 golden silk beans, 5-9 brown rice, 6-10 soybeans, 4-7 mung beans, 4-7 black rice, 6-9 millet, 5-10 red lentils, 3-5 barnyard grass seeds, 1-3 foxtail grass seeds, 2-5 wild millet, 1-3 canary seeds, 2-4 oats, 4-8 buckwheat, 2-6 chickpeas, 4-7 Job's tears, 2-4 rye flakes, 0.1-0.3 foxnut (for diuresis and dampness removal), 0.2-0.6 lily bulbs, 0.1-0.3 lotus seeds, 0.2-0.3 quinoa, 0.2-0.5 wild millet, and 0.2-0.5 spotted beans. According to this application, a multi-source grain and legume compound health cereal formula adopts a four-layer gradient scientific compound structure. The raw materials include conventional grains, legumes, native wild seeds and trace amounts of medicinal and edible ingredients. The nutrition is comprehensive and complementary, which can fully supplement the human body with high-quality plant protein, complex dietary fiber, rare minerals and amino acids.

The invention discloses an SSR molecular marker for identifying millet varieties and application of the SSR molecular marker. The invention provides a complete set of primers. The complete set of primers comprises at least 10, at least 20, at least 30 or all of primer pairs PM01 to PM40; the nucleotide sequences of the primer pair PM01 to the primer pair PM40 are sequence 1 to sequence 80 in sequence. Based on whole genome data of millet, a set of SSR marker combination with high polymorphism and high stability is screened and verified, and the SSR marker combination can cover the whole genome and can be used for rapidly and accurately assisting in distinguishing millet varieties. The method can promote standardization and intelligence of millet variety identification and promote healthy development of the seed industry.

The invention discloses a preparation method and application of pH response type premna microphylla polysaccharide-soybean protein isolate composite gel. The preparation method comprises the following steps: extracting premna microphylla polysaccharide, preparing a stock solution, preparing a soybean protein isolate solution, mixing the stock solution and the soybean protein isolate solution, adjusting the pH value to 3.0-9.0, heating, gelatinizing, cooling and curing to obtain the composite gel. The gel has the optimal performance under the acidic condition especially pH 3.0, the water binding capacity is greater than or equal to 62%, the storage modulus reaches 920Pa, the gel strength is high, the thermal stability is good, the structure is stable, a chemical cross-linking agent is not needed in the preparation process, and the raw materials are natural. The composite gel can be widely applied to the field of food processing, if the composite gel is used as a gel matrix, the water binding capacity, the taste stability and the nutritional value of a product can be effectively improved, and an effective solution is provided for high-value utilization of natural plant polysaccharide and protein and development of novel functional food.

The application discloses a preparation method of lactic acid bacteria fermented foxtail chub jelly. The application uses foxtail chub natural gum to replace artificial thickening agent, uses cane sugar and milk to prepare a mixed substrate, uses Lactobacillus bulgaricus, Lactobacillus acidophilus and Streptococcus thermophilus to prepare a lactic acid bacteria preparation, and performs combined fermentation to produce organic acid to replace citric acid to promote coagulation, without adding preservatives, so that the functional jelly with pure naturalness, high nutrition and excellent flavor is prepared.

The invention provides a production process of fried codonopsis pilosula with yellow millet, which is characterized in that the yellow millet is used as a frying medium to fry codonopsis pilosula nnannf. After being fried with the yellow millet, the codonopsis pilosula has a dark yellow surface and faint scent, the dryness of the codonopsis pilosula is removed, the codonopsis pilosula is brown in color, scorch-fragrant in taste, crisp and sweet in taste and free of residues during chewing, the effects of harmonizing the stomach, tonifying the spleen and stopping diarrhea are obviously enhanced, and the codonopsis pilosula is more suitable for people with insufficient immunity such as the old, the weak, infants and pregnant women; according to the traditional Chinese medicine property and flavor channel tropism theory, yellow millet produced from a genuine codonopsis pilosula nnannf place is used as a stir-frying medium instead of rhubarb millet, the drug property of the product is better met, and the application value of strengthening the spleen and stomach is highlighted; modern traditional Chinese medicine stir-frying equipment is adopted, the stir-frying temperature and stir-frying time requirements can be effectively mastered, and a standardized preparation technology is achieved.

The invention relates to the technical field of millet processing and production, and discloses a germinated millet production device and method. The germination accelerating and drying assembly is arranged in the shell, a plurality of germination accelerating and drying cabins are arranged in the germination accelerating and drying assembly in the vertical direction, and a material groove is formed in each germination accelerating and drying cabin; the germination accelerating and drying cabin can be used for accelerating germination of soaked millets in the germination accelerating and drying cabin and then drying the germinated millets, and the trough can be used for containing the soaked millets; the tiling mechanisms are mounted in the germination accelerating and drying cabins; the tiling mechanism comprises a linear motion assembly and a tiling assembly; the linear motion assembly can drive the tiling assembly so that the tiling assembly can move in the trough in the length direction of the trough, and the soaked millets in the trough can be tiled in the trough. According to the device, the trough is arranged, a site is provided for laying of soaked millets, and by arranging the laying mechanism, the soaked millets in the trough can be laid.

The invention discloses a special multilayer targeted release granular fertilizer for foxtail millet as well as preparation and application thereof, and solves the problems of supply and demand dislocation, low utilization rate, disjunction of prevention and control and fertilizer supply and poor loess soil fertilizer retention of the existing fertilizer. The fertilizer disclosed by the invention adopts a five-layer nested structure, corresponding to five growth periods of millet, each layer contains a functional core material and a customized coating, a montmorillonite isolating membrane is arranged between the layers, and 0.8% of an environmental response aid is added into the coating. The outer layer corresponds to fertilizer and damage prevention in the seedling stage, the middle layer adapts to jointing and booting stage requirements, and the core layer deals with high temperature in the heading and filling stage and lodging resistance in the mature stage. The particle size of the finished product is 8-10 mm, the finished product can be degraded after 3-6 months, the dosage of the finished product in a loess soil area is 310-320 kg / hm, and the whole growth period is covered with one-time basal application. After application, the utilization rate of the fertilizer is greater than or equal to 65%, the yield per mu is increased by 15-20%, the pesticide is reduced by 30-40%, and the method is suitable for northern areas, especially loess soil areas of elm

The invention discloses a molecular marker related to millet plant height and application of the molecular marker. The technical problem to be solved by the invention is to identify or assist in identifying the millet plant height. According to the application of the substance for detecting the polymorphism or genotype of the SNP in identifying or assisting in identifying the plant height of the foxtail millet, or preparing products for identifying or assisting in identifying the plant height of the foxtail millet, or breeding the foxtail millet or preparing products for breeding the foxtail millet, the SNP is a single nucleotide polymorphism site of a foxtail millet genome, is the 196th nucleotide of a sequence 1 in a sequence table, and is G or A. A variety of millet genomes are detected to find that the height of millet plants with the SNP genotype being GG is higher than that of millet plants with the SNP genotype being AA, or candidate millet plants are higher than that of millet plants with the SNP genotype being AA, and the method can be used for genetic breeding of millet plants.

This invention discloses an organic drip irrigation microbial fertilizer suitable for tomatoes, belonging to the field of agricultural microbial fertilizers. The fertilizer uses dandelion, purslane, barnyard grass, and foxtail grass as basic raw materials. These materials are prepared through directed tissue culture and mechanical-ultrasonic combined crushing, followed by enzymatic hydrolysis with mold to obtain a natural weed-derived culture medium. Then, Bacillus licheniformis is rapidly germinated using mid-wave infrared temperature control. Finally, a nutrient formula is precisely tailored to the tomato seedling, flowering, and fruit expansion stages, and a xanthan gum stabilization system is added to produce a liquid microbial fertilizer with a viscosity of 10-15 mPa·s to 20 mPa·s. This invention achieves high-value utilization of weed resources, solving problems such as cumbersome fermentation, unstable fertilizer effect, and poor nutrient compatibility in traditional microbial fertilizers. It improves the survival rate of bacterial strains and the utilization rate of raw materials, significantly reduces costs, allows for precise drip irrigation delivery, and significantly improves tomato survival rate, fruit set rate, and single fruit weight, thus offering both economic and environmental benefits.

The invention discloses a liquid-phase probe for millet genotyping, a method and application. The liquid-phase probe for millet genotyping is an imbricated probe with the length of 100-120 bp, and covers a target variation site and 50-70 bp regions on the upstream and downstream of the target variation site, and the target regions comprise a core gene region, a marker region, a regulation region and a genome skeleton region of millet. The probe and the method provided by the invention solve the problems of high cost and poor flexibility in the prior art, and realize large-scale, high-throughput and low-cost genetic typing of foxtail millet.