SSR (Simple Sequence Repeat) core primer group developed based on whole genome sequence of foxtail millet and application of SSR core primer group

A genome sequence and core primer technology, applied in the field of molecular biology, can solve the problem of the small number of SSR markers, achieve the effects of enriching polymorphism, promoting the improvement of millet genetic breeding level, and stable amplification

Active Publication Date: 2013-04-24
CROP RES INST SHANDONG ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with rice, wheat and other crops, the number of existing millet SSR markers is small, which cannot meet the needs of millet research. The development of a large number of SSR markers covering the whole genome is still one of the important tasks of millet research.

Method used

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  • SSR (Simple Sequence Repeat) core primer group developed based on whole genome sequence of foxtail millet and application of SSR core primer group
  • SSR (Simple Sequence Repeat) core primer group developed based on whole genome sequence of foxtail millet and application of SSR core primer group
  • SSR (Simple Sequence Repeat) core primer group developed based on whole genome sequence of foxtail millet and application of SSR core primer group

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Application of SSR Marker of Millet Genome in Variety Identification

[0023] 1. Extraction of millet genomic DNA

[0024] (1) Material

[0025] 41 representative local varieties and bred varieties were selected from the core germplasm materials of millet, covering 6 ecological regions in the Northeast Plain, North China Plain, South of Huaihe River, Loess Plateau, Inner Mongolia Plateau and Northwest Inland (see Table 1) , Used for the evaluation of millet primers and variety identification research.

[0026] Table 1 Information of materials used in the identification of millet varieties in the present invention

[0027]

[0028]

[0029]

[0030] (2) CTAB method to extract genomic DNA

[0031] The modified CTAB method is used to extract the DNA of millet, the specific steps are as follows:

[0032] 1) Sprouting and cultivating the tested millet materials, and extracting DNA when two leaves and one heart stage.

[0033] 2) Cut an appropriate amount of leaves, place them...

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Abstract

The invention discloses an SSR (Simple Sequence Repeat) core primer group developed based on whole genome sequence of foxtail millet and an application of the SSR core primer group, and belongs to the technical field of molecular biology. The core primer group comprises 30 pairs of primers, wherein nucleotide sequences are represented by SEQ ID NO. 1-60. The primer has advantages of clear electrophoretic band and rich polymorphism, and is uniformly distributed and stable in amplification. The invention also provides the application of the SSR core primer group in identifying the genetic diversity and variety of the foxtail millet. The primer group can be used for precisely and quickly identifying the variety of the foxtail millet and precisely reflecting a genetic relationship among the varieties of the foxtail millet.

Description

Technical field [0001] The invention relates to a millet SSR marker, specifically an SSR core primer set based on the whole genome sequence of millet and its application, and belongs to the technical field of molecular biology. Background technique [0002] Millet (Setaria Italica Beauv.) belongs to the Gramineae (Gramineae) genus Setaria (L.) Beauv., is the world’s oldest crop of Chinese origin and the nurturing crop of the Chinese nation. It used to feed the Chinese Revolution and the early days of New China. The problem played a very important role, and it was also the strategic food reserve at that time. Millet is rich in nutritional value, rich in vitamins A and E. The methionine in essential amino acids in humans is 118 times that of Bayi powder and 2125 times that of rice. It has good health care effects and can be developed and processed into a series of convenient foods. Cereal grain protein also has anti-atherosclerosis effects. [0003] Millet is a C4 plant. It has the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68C12N15/10
Inventor 李汝玉张晗王雪梅王东建姚凤霞许金芳孙加梅郑永胜段丽丽李华
Owner CROP RES INST SHANDONG ACAD OF AGRI SCI
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