143 results about "Camptotheca acuminata" patented technology

A processing method for protecting wood belongs to the technical field of antiseptic, mould proofing and insect prevention processing method, which is characterized in that the processing method comprises that adding NaOH or KOH in biomass gasification tars, mixing and stirring the mixture to react for 1-2 hours, then obtaining liquid A, crashing leaves and fruits of camptotheca acuminata decne to 50-200 courses to be mixed with aqueous solution of NaOH or KOH with weight of 5-20 times of camptotheca acuminata decne materials and consistency of 0.1-0.4%, after being stirred homogeneously, the mixture employs ultrasonic cell-break to react for 4-8 hours, after the slurry is filtered and concentrated, obtaining liquid B, the wood for treatment goes through dipping treatment by liquid A or liquid B which are diluted 20-100 times, then the wood after the dipping treatment goes through acidification dipping treatment. The processing method for protecting wood has antiseptic, mould proofing and insect prevention effects, includes no heavy metal which produces toxins to human beings and domestic animals, is safe for human being and causes no environmental pollution, which further is low in cost.

The invention discloses an anti-tumor traditional Chinese medicinal agent, which is prepared from the following raw materials in parts by weight: 10-20 parts of camptotheca acuminata, 5-10 parts of mockstrawberry lyophilized powder, 5-10 parts of fermented nostoc powder, 2-10 parts of toad, 30-40 parts of herba agrimoniae, 10-15 parts of a milk thistle extract, 10-15 parts of a solanum lyratum extract, 5-10 parts of bark of juglans mandshurica maxim, 10-15 parts of an olive leaf extract, 5-10 parts of a red jujube leaf extract, 5-10 parts of celery powder, 10-15 parts of a beet root extract, 10-15 parts of a blood orange extract, 6-10 parts of spreading hedyotis herb and 15-20 parts of radix stephaniae tetrandrae. The traditional Chinese medicinal agent disclosed by the invention has functions of removing toxins and eliminating stagnation, promoting blood circulation and removing stasis, benefiting qi and nourishing yin, killing and injuring tumor cells, inhibiting growth of cancer cells, controlling disease development and boosting immunity. The medicine, which is generally prepared from natural ingredients, is safe to take and free from obvious side effects; and based upon clinical observation for years, no adverse reaction occurs in patients; therefore, the medicine is suitable for the patients to take for a long time.

The invention is a high-content camptothecin callus cultivating method of camptotheca acuminata, its steps including: (1) prepare outer plant body; (2) induce the callus; cut the happy-tree laminae into blocks of 0.5X0.5-1.0 cm2, and the leafstalk cut into 0.5-1.0 cm segments inserted in the callus-inducing culture medium for inducing. The above culture medium is prepared by adding sucrose, 20-40g, agar powder, 5.4-7.2g, 2,4-dichlorophenoxyacetic acid, 0.25-3.0 mg, and 6-(benzyl amino) purine, 0.5-3.0 mg in 1 litre of MS basic culture medium, pH 6.0. Hold the prepared culture medium in the nutritious bottles, 25ml/bottle, then sterilize for 25 minutes under a 0.1-0.15 MPa pressure, then place the bottles in the hothouse for cultivating, temperature 22-26 deg.C and luminous intensity 2000-3000 lx.

The present invention relates to the method of promoting the formation of 10-hydroxyl camptothecine in camptotheca seed sprout. The technological process includes water culture of camptotheca seed for sprouting, ultrasonic radiating sprouting camptotheca seed in an ultrasonic device with water, crushing and homogenating the seed sprout tissue in a homogenating machine, filtering to obtain filtrate of 10-hydroxyl camptothecine. The said process has the features of low cost and high yield of 10-hydroxyl camptothecine.

The invention relates to a Chinese medicinal herb adhering corsage for preventing and treating the mammary diseases, comprising a corsage which can cover the chest and the back of a female and is madeof soft cotton cloth materials. Parts of the inner surface of the corsage corresponding to chest breast acupuncture point and thoracic lymph are respectively provided with a medical physical therapypatch dipped and treated by the Chinese medicinal herb solution, and the Chinese medicinal herb solution is prepared from medicine raw materials f saffron, pseudo-ginseng, rhubarb, leech, asarum, ground beeltle, camptotheca acuminata nuts and the like and water according to a certain proportion. The whole prescription has the functions of dispersing stagnated hepatoqi, activating blood and dissolving stasis, reducing swelling and easing pain and diminishing inflammation, has excellent effect of treating the female mammary diseases and can remarkably reduce or eliminate the threat and the harmfulness of the diseases to women health.

The invention discloses a botanical fungicide for anthracnose. The botanical fungicide comprises the following ingredients by weight: 12-22 parts of a fig extract, 15-25 parts of a solanum torvum extract, 20-30 parts of a thesioides extract, 5-15 parts of tabasheer, 8-18 parts of crinis carbonisatus, 21-32 parts of black tin, 3-10 parts of camptotheca acuminata and 7-17 parts of herba polygoni avicularis. According to the technical scheme, the botanical fungicide is free of pollution and residue, safe and environmentally friendly, and the possibility of resistance to the botanical fungicide is low.

The invention discloses a nutrition protective agent capable of promoting content of camptothecin in camptotheca acuminata to increase, and belongs to the technical field of nutrition of trees. The nutrition protective agent is characterized by comprising the following substances in percentage by weight: 13 to 37 percent of nutritional ingredient, 4 to 13 percent of growth hormone, 2 to 9 percent of preservative, 1 to 7 percent of synergistic agent and 43 to 71 percent of water. The nutrition protective agent capable of promoting the content of the camptothecin in the camptotheca acuminata to increase has various effects of promoting the content of the camptothecin in camptotheca acuminata fruits, camptotheca acuminata leaves and camptotheca acuminata branches to increase, the camptotheca acuminata to grow new roots and branches, the nursery stock and the branches of the camptotheca acuminata to extend and thicken and the camptotheca acuminata fruits to increase, can effectively prevent various diseases and pests of the trees and has a special effect of promoting the content of the camptothecin in the camptotheca acuminata to increase. The nutrition protective agent is easy and convenient to process, low in equipment investment and low in production cost, can be safely stored, transported and used and cannot pollute the environment, sources of raw materials are ensured, and the energy consumption is reduced.

The high-effective production method of 10-hydroxycamptothecin includes the following steps: making seed of camptotheca acuminate undergo the processes of negative pressure cavitation mixed-rotating wall-breaking, disinfecting, removing impurity, water culture and germination, mixing the seed sprout and extraction solvent, homogenization and solid-liquid extraction, filtration, negative pressure cavitation mixed-rotating solid-liquid extraction, filtration, concentration, negative pressure cavitation mixed-rotating liquid-liquid extraction, recrystallization or silicon gel column chromatography so as to obtain the 10-hydroxycamptothecin. Said invention raises its yield, can be substituted for traditional various extraction methods of drying material, pulverizing and heating process.

The invention belongs to technical filed of biology, in particular to a method for promoting biosynthesis of 10-hydroxycamptothecine. The method comprises the following steps of: constructing a suspension culture system of camptotheca acuminata cells for an explant by using leaves of camptotheca acuminata plants; adding three kinds of exogenous substances such as cobalt nitrate, lanthanum nitrate and salicylic acid to regulate and control the secondary metabolism of the camptotheca acuminata cells; and inoculating two kinds of microorganisms such as Mucor spinosus and Aspergillus niger for biotransformation at the later stage of cell culture, placing the treated camptotheca acuminata cell culture into a pulp refiner, into which an ethanol solution is added, for homogenating, and filtering to obtain filtrate containing 10-hydroxycamptothecine. The method has the characteristics of short production period, low production cost and high yield of 10-hydroxycamptothecine, and is beneficial to the protection of the camptotheca acuminata plant resources.

The invention relates to a camptotheca endophytic bacterium which is obtained from camptotheca acuminate through separation and purification, and an application of the camptotheca endophytic bacterium. The camptotheca endophytic bacterium LY214 involved by the invention is identified to be paenibacillus polymyxa by morphology and molecular biology; and the bacterial strain is preserved in the China General Microbiological Culture Collection Center, the preservation date is November 19th, 2012, and the preservation number is CGMCC 6841. The camptotheca endophytic bacterium is characterized in that the high performance liquid chromatography, the ultraviolet spectrum and the mass spectrometry show that the camptotheca endophytic bacterium can generate an antineoplastic active component which is camptothecin through liquid fermentation. The invention discloses for the first time that the endophytic bacterium for producing camptothecin can be separated and cultured from the camptotheca acuminate, and provides a new way for the preparation of camptothecin.

The invention discloses a Chinese medicament preparation for treating lymphoma and a preparation method of the Chinese medicament preparation, belonging to the field of Chinese medicaments. Effective components of the Chinese medicament preparation disclosed by the invention comprise raw materials including airpotato yam rhizome, dindygulen peperomia herb, polygonum chinensis, sal ammoniac, barbed skullcap herb, oxystelma esculentum, madagascar periwinkle herb, camptotheca acuminata, echelette, marsdenia tenacissima, tender catchweed bedstraw herb, siberian solomonseal rhizome, black nightshade herb, Chinese sage herb, Japanese honeysuckle stem and salvia miltiorrhiza. The Chinese medicament preparation disclosed by the invention selects medicinal materials with appropriate compatibility, is prepared by selecting Chinese medicinal material compositions capable of preventing cancer and lowering blood pressure, dispersing swelling and resolving masses, detoxifying and removing blood stasis and supporting the healthy energy, has the effects of inducing diuresis and removing edema, removing blood stasis and relieving pain and activating blood circulation and stimulating meridians without adverse reactions and side effects; through clinical verification, the Chinese medicament preparation has extremely good relief and treatment effects on lymphoma, and is suitable for the popularization and application in clinical treatment and nursing of lymphoma.

The invention provides a natural plant innoxious mosquito expelling perfume. The natural plant innoxious mosquito expelling perfume is composed of the following components in mass percent: 25% of camptotheca acuminata roots, 40% of camptotheca acuminata leaves, 5.9 % of plant essence, 0.1% of edible preservative agent, and the balance of distilled water. The natural plant innoxious mosquito expelling perfume provided by the invention has the advantages that as the main components including the camptotheca acuminata roots, the camptotheca acuminata leaves and the plant essence are pure natural botanical compositions, the made-up mosquito expelling perfume has no toxic and side effects, causes no damage on a human body and environmental pollution, and has a good mosquito expelling effect.

The invention discloses a Japan snakeroot strictosidine synthase gene, protein which is encoded by the Japan snakeroot strictosidine synthase gene and the use thereof. The Japan snakeroot strictosidine synthase gene which is provided by the invention has a nucleotide sequence or a homologous sequence which adds, replaces, inserts or losses one or a plurality of nucleotides or allele thereof and the nucleotide sequence which is derived from the Japan snakeroot strictosidine synthase gene, which are displayed in the SED ID No.1. The protein which is encoded by the gene has an amino acid sequence or the homologous sequence which adds, replaces, inserts or losses one or a plurality of amino acids, which is displayed in the SEQ ID No.2. The Japan snakeroot strictosidine synthase gene which is provided by the invention can increase the content of camptothecin which is an anticancer component in resource plants such as Japan snakeroot or camptotheca and the like through the genetic engineering technology and can be used in research and industrialization for increasing the content of the camptothecin through utilizing the transgenic technology, which is helpful for accelerating to solve the problem that the drug resources of the camptothecin are seriously scarce and has very good application prospect.

The invention discloses a traditional Chinese medicine composition for tonifying qi and blood and resisting cancer, and raw materials is composed of the following compositions in parts by mass: 12-20 parts of panax ginseng, 6-15 parts of rhizoma atractylodis macrocephalae, 8-12 parts of radix glycyrrhizae, 7-13 parts of poria, 12-17 parts of radix angelicae sinensis, 10-14 parts of rhizoma chuanxiong, 10-15 parts of radix paeoniae alba, 12-18 parts of radix rehmanniae preparata, 13-16 parts of radix astragali, 18-22 parts of radix maclurae, 28-34 parts of rhizoma imperatae, 28-35 parts of reed rhizosphere, 28-35 parts of salix babylonica root, and 8-10 parts of fructus camptothecae acuminatae. The traditional Chinese medicine composition helps to support right and secure the root by tonifying qi and blood, and also helps to enhance patient organism immunity and selectively kill cancer cells, and has substantial medicinal efficacy for treating leukemia, osteocarcinoma, lymphatic sarcoma, breast cancer, skin cancer, gastric cancer and esophagus cancer.

The invention discloses a biological pesticide for preventing and controlling strawberry powdery mildew. The biological pesticide is prepared from traditional Chinese herbal medicaments cloves, fallopia multiflora, celosia cristata, radix ranunculi ternati, sharpleaf galangal fruits, polygonatum, camptotheca acuminata and coral head plant seeds. The biological pesticide is biologically measured to have a strawberry powdery mildew germ suppression rate of over 80 percent and remarkable effects; a field test shows that prevention and control effects on strawberry powdery mildew after the pesticide is applied for three times are 60 percent, 70 percent and 80 percent respectively, are better than 50 percent carbendazim, and tend to be improved along with the increase of pesticide application times, and the biological pesticide can act for a long time.

The invention belongs to the field of biotechnology, and particularly relates to a microbial transformation preparation method of hydroxycamptothecin. The invention conducts biotransformation to the camptotheca acuminata cells of a suspension culture by using non-toxic Aspergillus flavus Cr-1 strains (Aspergillus.flavus) and Rhizopus T-34 strains (Rhizopus sp.); the camptotheca acuminate cell cultures after the transformation experience separation and refining such as raw material crushing, reflex extraction, crystallization, silica gel column chromatography, crystallization, and the like, a pure hydroxycamptothecin product is obtained. The invention conducts biotransformation to the camptotheca acuminata cells of the suspension culture by using a microorganism so as to obtain a plant secondary metabolite hydroxycamptothecin which has an anti-tumor activity, wherein, the content of the plant secondary metabolite hydroxycamptothecin achieves 0.4% (g/g) of the dry weight of the cell; a new way of obtaining high-yield hydroxycamptothecin is established, thereby meeting the requirements of sustainable development; the method for separating the hydroxycamptothecin is simple, convenient and feasible; the purity of the product is high; therefore, the invention has good development prospect.

The invention discloses a novel environment-friendly nontoxic pesticide which comprises a propellent and a compactable gas. The novel environment-friendly nontoxic pesticide is characterized by also comprising a mixed liquid extracted from water, pyrethrum, derris, mint, licorice, pumpkin seed, camptotheca acuminata decaisne root peel and garlic according to a mass ratio of 30:2:0.5:0.3:0.6:0.8:3:6. The pesticide is completely prepared by plants, has no any chemical components, is not harmful to human body, guarantees people health and is extremely practical.

The present invention aims that by using the excellent bacteria strain screened from Camptotheca acuminata leaf or seed in different rot degree as strain for production, the residue after camptothecine extraction is air dried, alcohol evaporated, mixed with wheat bran, cane sugar, calcium sulfate, urea and water, and fermented at high temperature to form organic fertilizer capable of being utilized by plant. The present invention can eliminate the environmental pollution caused by the residue after camptothecine extraction.

The invention discloses a cutting rooting method for epicormic branches of camptotheca acuminate. The cutting rooting method comprises the following steps: cutting the branches from a big tree and taking as cutting slips, wrapping with a piece of wet cloth and keeping fresh state; straightly cutting the upper notches of the cutting slips, bias cutting the lower notches, maintaining 1-2 leaves on the upper part and cutting off 1/2-2/3 of the leaves; disinfecting for 2h by adopting 0.1% carbendazim solution, and then treating for 20s-2h by adopting a hormone solution; performing cuttage for epicormic branches on a substrate, and by keeping 8-10cm seed spacing, maintaining the depth of the cutting slips as about 1/2 of the length and exposing the first leaf on the substrate; watering after finishing the cuttage; performing labor management, and then performing intertillage weeding per two weeks and spraying 0.1% carbendazim solution for disinfecting; lignifying the roots of the camptotheca acuminate, and then transplanting in time. According to the cutting rooting method for epicormic branches of camptotheca acuminate provided by the invention, a camptotheca acuminate clone seedling system is established, so that the high-quality camptotheca acuminate seedlings are acquired. The method is simple and convenient in operation, is feasible and has the advantages of low cost, simple technical requirement, easiness in popularization and application, and the like.

The invention belongs to the technical field of genetic engineering, particularly relates to a method for increasing nicotine content in tobacco by utilizing camptotheca acuminate AOC (Allene Oxide Cyclase) gene transformation. In the method, a plant expression vector is constructed by using camptotheca acuminate allene oxide cyclase (AOC) genes; and the nicotine content in the tobacco is increased through over-expression of the camptotheca acuminate AOC genes in the tobacco by using a genetic engineering technology. The method comprises the process of: cloning the AOC genes from camptotheca acuminate; constructing a plant high-efficiency expression vector; introducing agrobacterium tumefaciens and performing genetic transformation on the tobacco; and measuring the nicotine content in transgenic tobacco leaves. By adopting the method, the camptotheca acuminate AOC genes can be over-expressed in the tobacco, and the nicotine content in the tobacco is increased.

The invention discloses an anticancer aroma-enhancing filter composite for smoking, comprising the following substances in parts by mass: 2-3 parts of taxus, 0.5-1 part of zedoary, 0.5-1 part of jobs tears, 0.5-1 part of catharanthus roseus, 0.5-1 part of cephalotaxus fortune, 0.5-1 part of dysosma versipellis, 0.5-1 part of rabdosia rubescens, 0.5-1 part of meadow saffron, 0.5-1 part of camptotheca acuminata decne and 0.5-1 part of brucea javanica. Through the anticancer aroma-enhancing filter composite for smoking, provided by the invention, a lot of carcinogenic substances such as nicotine, neonicotine and the like in the smog generated by tobacco combustion can be reacted and neutralized; after a small quantity of carcinogenic substances are inhaled, as a lot of anticancer substances and substances for strengthening the immunity are inhaled, the harm to the human organs, caused by the carcinogenic substances, are greatly decreased, and the harm to the human body, caused by the smog, is minimized; and the original flavor of the tobacco is not destroyed by simply and largely adsorbing the smog, and meanwhile, a lot of plant spices are added, so that the anticancer aroma-enhancing filter composite for smoking has purer taste and can be accepted more easily.

The present invention provides a compound botanical insecticide for mosquito larvae control, a preparation method therefor and applications thereof. The insecticide consists of a bulk drug and conventional auxiliaries, wherein the mass of the bulk drug is 40-80% of the mass of the insecticide, and the mass of the conventional auxiliaries is 20-60% of the mass of the insecticide. The bulk drug comprises the following components in weight percentages based on the total weight of the bulk drug: 2-20% of a tomato extract, 5-30% of a garlic extract, 5-30% of a Euphorbia humifusa extract, 5-10% of a ginger extract, 5-25% of a potato extract, 5-30% of a Cinnamomum camphora extract, 5-20% of a photinia extract, 5-15% of a Liriodendron chinense extract, and 4-10% of a Camptotheca acuminata extract. The compound botanical insecticide for mosquito larvae control provided by the present invention is high in efficiency, free of residuals, low in production cost, economical and practical, environmentally-friendly, and excellent in comprehensive properties, and comprises agricultural wastes.

The invention relates to the technical field of food and discloses spicy rabbit meat. The spicy rabbit meat is prepared from the following raw materials: rabbit meat, white granulated sugar, beer yeast, honey, salt, spice, common camptotheca fruit extract and lithospermum extract. The spicy rabbit meat is spicy and tasty, is moderate in salty and sweet flavor, is strong in fragrance, is free from meat smell, contains less fat and is capable of protecting heart and cerebral vessels; according to a cooking method for abundant rabbit meat, the rabbit meat is soaked with edible aqueous alkali, the smell is faded and the meat is tender; the meat is easily cooked; the rabbit meat is fermented with the beer yeast; the residual pathogenic microorganism can be killed; the shelf life is prolonged.