4 results about "Camptotheca acuminata" patented technology

This invention discloses a pharmaceutical composition and its preparation method for treating atrophic gastritis, precancerous lesions of the stomach, and stomach cancer, belonging to the field of traditional Chinese medicine preparation technology. The pharmaceutical composition is prepared by a specific two-step microbial fermentation process using Astragalus membranaceus, Hericium erinaceus, Curcuma zedoaria, Actinidia chinensis root, Camptotheca acuminata fruit, Hedyotis diffusa, Coptis chinensis, Taraxacum mongolicum, Viola yedoensis, Corydalis yanhusuo (processed with vinegar), Phellodendron chinense, Solanum nigrum, Scutellaria baicalensis, Panax notoginseng, Zingiber officinale, Piper cubeba, Cuttlebone, Fritillaria thunbergii, roasted soybean, and red yeast rice. This invention significantly improves the bioavailability of the active pharmaceutical ingredients through the synergistic effect of the multifunctional groups of traditional Chinese medicine and the enhanced effect of microbial fermentation. It can systematically reverse gastric mucosal atrophy and intestinal metaplasia, effectively improve microcirculation, and inhibit Helicobacter pylori, while also exhibiting good safety. This provides a novel and effective treatment for the aforementioned gastric diseases.

The application belongs to the technical field of traditional Chinese medicine, and specifically discloses a traditional Chinese medicine preparation for treating sepsis and a preparation method thereof. The traditional Chinese medicine preparation comprises the following raw materials in parts by weight: Fructus camptothecae 20-25 parts, Semen persicae 15-20 parts, Pericarpium trichosanthis 10-15 parts, Elatostema umbellatum 12-15 parts, Radix baiyuan 10-15 parts, Radix codonopsis 6-10 parts, Semen amygdali 5-10 parts, Radix notoginseng 6-10 parts, and Radix glycyrrhizae 4-8 parts. The traditional Chinese medicine preparation provided by the application has a reasonable prescription, and the monarch, the minister, the assistant and the messenger complement each other. In the prescription, Fructus camptothecae and Semen persicae are used as monarch drugs to play the efficacy of promoting blood circulation and removing blood stasis; Pericarpium trichosanthis, Elatostema umbellatum and Radix baiyuan are used as minister drugs, which, in combination with the monarch drugs, can play the efficacy of clearing heat and detoxifying while enhancing the efficacy of promoting blood circulation and removing blood stasis; Radix codonopsis, Radix notoginseng and Semen amygdali are used as assistant drugs to tonify the middle and replenish qi and blood, and Radix glycyrrhizae is used as messenger drug to harmonize various drugs, guide drugs into channels, and expel evil. The whole prescription has a reasonable compatibility, and has a good therapeutic effect on sepsis.

This invention discloses a method for preparing a seven-membered cucurbita supramolecular cocrystal solvent and its application. The invention uses a seven-membered cucurbita as a hydrogen bond acceptor and malic acid as a hydrogen bond donor to prepare a seven-membered cucurbita supramolecular cocrystal solvent. This solvent can be used to extract camptothecin from Camptotheca acuminata fruit, and the extracted camptothecin can be determined by HPLC. This seven-membered cucurbita supramolecular cocrystal solvent is a green extraction solvent that does not use organic solvents when extracting active ingredients from plants, while the extracted active ingredients retain their intrinsic activity. The preparation method is simple, the extraction efficiency is high, and efficient, green extraction is achieved.

This invention provides an application of the camptothecin oxygen methyltransferase CaOMT3 in catalyzing the oxygen methylation modification of hydroxycamptothecin and quercetin. Through transcriptomic and genomic analysis, four oxygen methyltransferases (CaOMT3) were identified. BECAUSE OF Genes were found to be clustered together; further analysis of metabolite and transcriptome co-expression revealed a gene significantly positively correlated with rhamnine accumulation. BECAUSE OF Genes, that is CaOMT3 Biochemical functional verification showed that CaOMT1, CaOMT2, and CaOMT3 can all catalyze the reaction of 9- and 10-hydroxycamptothecin. O Methylation yields the corresponding methoxycamptothecin. When quercetin is used as a substrate, CaOMT1 and CaOMT3 exhibit bifunctional catalytic activity, capable of simultaneously catalyzing the methylation of the 4'- and 7'-hydroxyl sites. O α-methylation yields rhamnosin, tamarindin, and phytoflavin; while CaOMT2 only catalyzes the 4'-hydroxyl site. Enzymatic kinetic analysis showed that CaOMT3's catalytic efficiency for quercetin ( k cat / K (m) is the highest. This invention provides a new enzyme tool and technological foundation for the synthetic biology research and industrial fermentation production of rhamnine and methoxycamptothecin.