18 results about "Camptotheca acuminata" patented technology

The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine composition with a hypoglycemic effect and a preparation method thereof. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 15-23 parts of astragalus membranaceus, 12-18 parts of sambucus chinensis root, 10-15 parts of dogwood, 5-8 parts of wormwood stem, 1-5 parts of camptotheca acuminata fruit, 1-5 parts of cyclocarya paliurus, 3-8 parts of kudzu vine root, 1-5 parts of mulberry leaf and 1-3 parts of honey-fried licorice root. All the components of the traditional Chinese medicine composition are compatible according to a certain proportion, the traditional Chinese medicine composition can tonify qi and raise yang, unblock triple jiao, dispel and strengthen evil, activate blood and dredge vessels, jointly achieve the effects of tonifying qi and nourishing, promoting qi circulation to relieve pain and tonifying kidney and dredging collaterals, can reduce the blood sugar content, protect the cardiovascular system and achieve the curative effect of preventing and treating diabetes, and has practicability and application value.

The invention discloses a traditional Chinese medicine composition for treating esophageal cancer. The traditional Chinese medicine composition is prepared from, by weight, 180-210 g of prepared rehmannia roots, 120-150 g of radix clematidis, 100-120 g of rhizoma atractylodis macrocephalae, 120-150 g of fructus lycii, 120-150 g of semen cuscutae, 100-120 g of rhizoma alismatis, 100-120 g of rhizoma dioscoreae, 40-60 g of taxus chinensis, 100-120 g of codonopsis pilosula, 20-40 g of scorpions, 40-60 g of curcuma zedoary, 60-80 g of peach kernels, 120-150 g of salvia chinensis, 120-150 g of rabdosia rubescens, 90-120 g of Chinese dates, 100-120 g of camptotheca acuminate, 30-50 g of ground beetles, 60-80 g of pseudo-ginseng The composition has the effect of treating both symptoms and root causes in the aspect of treating esophageal cancer, can control tumors, improve the living quality of patients and prolong survival, and is small in side effect.

The invention belongs to the field of plant tissue culture, and particularly relates to a method for promoting accumulation of camptothecin by overexpressing tryptophan synthetase. The invention provides a method for promoting camptothecin accumulation of camptotheca acuminate cells, which comprises the following steps: constructing camptotheca acuminate cells overexpressing tryptophan synthetase genes, performing tissue culture amplification, and extracting camptothecin from the amplified camptotheca acuminate cells. The method for overexpressing the tryptophan synthetase gene is superior to a method for directly adding tryptophan into a culture medium, and the camptothecin yield can be increased by 2.33 times.

The method comprises the following steps of: firstly, rubbing and pinching a patient lying on a bed by hands for more than ten minutes, smearing an external ointment on the back, applying iron while the ointment is hot, and covering the ointment with a plastic film for more than ten minutes until the patient cannot catch a cold by blowing; and 2, regulating healthy qi of five internal organs by using a medicine and food homologous formula, recovering internal circulation, supplementing nutrition and enhancing resistance. And third polymerization. The patients under the elbows and the knee joints can dredge channels and collaterals through needle reinforcing and reducing, and the qi and blood smoothness effect of the patients is improved. And 4, mixing Chinese herbal medicines, cordate houttuynia, lalang grass rhizome, camptotheca acuminate fruit, Chinese lobelia, sedum sarmentosum, tuckahoe papaya, salvia chinensis, liquorice and Chinese yam to obtain secret formula wall-broken powder with the size of 300-800 meshes, brewing the powder with boiled water to obtain tea, and drinking the tea, so that the tea can be doubled to be absorbed by the body of the patient. The externally applied ointment is prepared from eucommia ulmoides, ligusticum wallichii, radix achyranthis bidentatae, angelica sinensis, cinnamon and dogwood pulp. , salvia chinensis, rehmannia glutinosa, elderberry and sambucus chinensis. , rhizoma drynariae, frankincense and myrrh. The raw materials are prepared by a special processing method for later use.

This invention discloses a pharmaceutical composition and its preparation method for treating atrophic gastritis, precancerous lesions of the stomach, and stomach cancer, belonging to the field of traditional Chinese medicine preparation technology. The pharmaceutical composition is prepared by a specific two-step microbial fermentation process using Astragalus membranaceus, Hericium erinaceus, Curcuma zedoaria, Actinidia chinensis root, Camptotheca acuminata fruit, Hedyotis diffusa, Coptis chinensis, Taraxacum mongolicum, Viola yedoensis, Corydalis yanhusuo (processed with vinegar), Phellodendron chinense, Solanum nigrum, Scutellaria baicalensis, Panax notoginseng, Zingiber officinale, Piper cubeba, Cuttlebone, Fritillaria thunbergii, roasted soybean, and red yeast rice. This invention significantly improves the bioavailability of the active pharmaceutical ingredients through the synergistic effect of the multifunctional groups of traditional Chinese medicine and the enhanced effect of microbial fermentation. It can systematically reverse gastric mucosal atrophy and intestinal metaplasia, effectively improve microcirculation, and inhibit Helicobacter pylori, while also exhibiting good safety. This provides a novel and effective treatment for the aforementioned gastric diseases.

The present application relates to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition with hypoglycemic effect and a preparation method thereof.The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 15-23 parts of Astragalus membranaceus, 12-18 parts of Sambucus williamsii root, 10-15 parts of Fructus Corni, 5-8 parts of Artemisia argyi stem, 1-5 parts of Fructus Camptothecae, 1-5 parts of Rhamnus utilis, 3-8 parts of Pueraria lobata, 1-5 parts of Morus alba leaf, and 1-3 parts of Radix Glycyrrhizae.The components of the traditional Chinese medicine composition are compatible with each other according to a certain ratio, can tonify qi and raise yang, dredge Sanjiao, expel evil, promote blood circulation and unblock collaterals, jointly achieve the effects of tonifying qi and nourishing, promoting qi and relieving pain, and tonifying kidney and dredging collaterals, can reduce blood sugar content, protect the cardiovascular system, achieve the effects of preventing and treating diabetes, and have practicality and application value.

The application belongs to the field of Zhuang and Yao medicine treatment of acute ischemic stroke, and discloses a Zhuang and Yao medicine compound medicine, a preparation method and application thereof in treating acute ischemic stroke. The Zhuang and Yao medicine compound medicine comprises the following raw materials in parts by weight: Ficus tikoua 55-62 parts, Camptotheca acuminata 12-17 parts, Ilex hainanensis 26-35 parts and Saposhnikovia divaricata 13-18 parts. The four raw materials are put into clean water, and the raw materials are prepared into a medicinal liquid to obtain the Zhuang and Yao medicine compound medicine. The Zhuang and Yao medicine compound medicine is cheap, convenient to use, has remarkable curative effect, can dredge the dragon road and the toxic evil and toxic side effects of the fire road, and has a simple preparation process.

The invention relates to the technical field of extraction and enrichment of natural products, in particular to a green extraction and efficient enrichment method of camptothecin (CPT) and 10-hydroxycamptothecin (HCPT). The problems of low efficiency, poor safety, high cost and the like in the common technology are solved. The method comprises the following steps: mixing dried camptotheca acuminate tissue powder with a nonionic surfactant Genapol X-080 according to a certain solid-to-liquid ratio, extracting in extraction equipment, and centrifuging after extraction to obtain CPT and HCPT extracting solutions; adding inorganic salt into the extracting solution at a certain temperature, uniformly stirring, carrying out directional enrichment treatment for a certain time, layering the solution by centrifuging or standing, and taking an upper-layer surfactant-rich phase, so as to obtain an enriched product of the CPT and HCPT extracting solution. According to the method, no organic solvent is used in the extraction and enrichment process, the cost is low, safety and reliability are achieved, high economic benefits and environmental benefits are achieved, good application and popularization prospects are achieved, and the method has important significance on industrial safety production of CPT and HCPT.

The application belongs to the technical field of traditional Chinese medicine, and specifically discloses a traditional Chinese medicine preparation for treating sepsis and a preparation method thereof. The traditional Chinese medicine preparation comprises the following raw materials in parts by weight: Fructus camptothecae 20-25 parts, Semen persicae 15-20 parts, Pericarpium trichosanthis 10-15 parts, Elatostema umbellatum 12-15 parts, Radix baiyuan 10-15 parts, Radix codonopsis 6-10 parts, Semen amygdali 5-10 parts, Radix notoginseng 6-10 parts, and Radix glycyrrhizae 4-8 parts. The traditional Chinese medicine preparation provided by the application has a reasonable prescription, and the monarch, the minister, the assistant and the messenger complement each other. In the prescription, Fructus camptothecae and Semen persicae are used as monarch drugs to play the efficacy of promoting blood circulation and removing blood stasis; Pericarpium trichosanthis, Elatostema umbellatum and Radix baiyuan are used as minister drugs, which, in combination with the monarch drugs, can play the efficacy of clearing heat and detoxifying while enhancing the efficacy of promoting blood circulation and removing blood stasis; Radix codonopsis, Radix notoginseng and Semen amygdali are used as assistant drugs to tonify the middle and replenish qi and blood, and Radix glycyrrhizae is used as messenger drug to harmonize various drugs, guide drugs into channels, and expel evil. The whole prescription has a reasonable compatibility, and has a good therapeutic effect on sepsis.

The invention discloses a Kangpu tea fermentation device, relates to the technical field of fermentation engineering, and is characterized in that a fixed plate is mounted at the upper end of a fermentation cavity of a fermentation tank body. The upper space of the fermentation cavity is separated by the fixed plate, and a gas collection chamber is formed on one side, far away from the fermentation cavity, of the fixed plate. And a gas guide piece, a sealing cylinder, a transmission structure and a cleaning plate are arranged on the gas collection chamber. A plurality of vent holes are formed in the fixed plate and are used for communicating the fermentation cavity below with the gas collection cavity above. One end, close to the fermentation cavity, of each vent hole is provided with an energy storage part which is telescopic based on gas pressure, and one end, relatively close to the gas collection cavity, of each vent hole is provided with a slidable sealing cover. The energy storage part is used for sensing the air pressure change in the fermentation cavity and driving the sealing cover, so that the opening and closing of the vent hole are controlled. According to the invention, the cleaning plate is driven based on discharged gas to automatically scrape mycoderm and sediment attached to the tank wall, so that the self-cleaning function of the fermentation device is realized.

The invention provides a flavanone C3-hydroxylase gene of camptotheca acuminate, a carrier, a protein and application, and belongs to the technical field of bioengineering.CYP71A hydroxylase genes CYP71AU223 and CYP71AU224 in the camptotheca acuminate are obtained through screening, plasmids are constructed and introduced into a saccharomyces cerevisiae WAT11 host cell, the host cell is used for inducing expression of two microsomal proteins of CYP71AU223 and CYP71AU224, and therefore the flavanone C3-hydroxylase gene of the camptotheca acuminate is obtained. The two microsomal proteins both show C-3 site hydroxylation activity on the key intermediate naringenin for flavone biosynthesis, and can be used for preparing the citrus aurantium. According to the invention, molecular docking and site-specific mutagenesis technologies are combined to determine substrate oriented stable key residues of two proteins CYP71AU223 and CYP71AU224, the catalytic activity of the key residues is improved, and the types of hydroxylase at C3 site of flavonoid compounds are enriched.

This invention discloses a method for preparing a seven-membered cucurbita supramolecular cocrystal solvent and its application. The invention uses a seven-membered cucurbita as a hydrogen bond acceptor and malic acid as a hydrogen bond donor to prepare a seven-membered cucurbita supramolecular cocrystal solvent. This solvent can be used to extract camptothecin from Camptotheca acuminata fruit, and the extracted camptothecin can be determined by HPLC. This seven-membered cucurbita supramolecular cocrystal solvent is a green extraction solvent that does not use organic solvents when extracting active ingredients from plants, while the extracted active ingredients retain their intrinsic activity. The preparation method is simple, the extraction efficiency is high, and efficient, green extraction is achieved.

The present application relates to the technical field of natural product extraction and enrichment, and particularly relates to a method for extracting and enriching camptothecin (CPT) and 10-hydroxyl camptothecin (HCPT). The method solves the problems of low efficiency, poor safety, high cost and the like of common technologies. Dry camptothecin tissue powder and non-ionic surfactant Genapol X-080 are mixed according to a certain solid-liquid ratio, and then are placed in an extraction device for extraction. After extraction, CPT and HCPT extraction liquid is obtained through centrifugation. Inorganic salt is added to the extraction liquid at a certain temperature, and is uniformly stirred. The solution is subjected to directional enrichment treatment for a certain time, and is subjected to centrifugation or standing to separate the solution into layers. The upper surfactant-rich phase is taken, and the enrichment product of the CPT and HCPT extraction liquid is obtained. The extraction and enrichment process of the present application does not use any organic solvent, and is low in cost, safe and reliable, has high economic and environmental benefits, and has a good popularization and application prospect. The method has important significance for the industrialized safe production of CPT and HCPT.

The present application relates to the field of plant tissue culture, and particularly discloses a high-efficiency in-vitro accumulation method of camptothecin. The present application uses young stem segments of camptotheca acuminata as explants, and induces accumulation of camptothecin in camptotheca acuminata whole plants through the steps of bud induction, adventitious bud induction, camptothecin in-vitro enrichment phase I culture, camptothecin in-vitro enrichment phase II culture, camptothecin in-vitro enrichment phase III culture, and adventitious root camptothecin in-vitro enrichment, thereby establishing a high-efficiency in-vitro accumulation method of camptothecin. The method of the present application can produce camptothecin under in-vitro conditions, and meets the requirements of season-unrestricted production of camptothecin, land saving, and natural camptothecin without toxic and side residues.

The invention belongs to the technical field of traditional Chinese medicines, and particularly discloses a traditional Chinese medicine preparation for treating sepsis and a preparation method thereof. The traditional Chinese medicine preparation is prepared from the following raw materials in parts by weight: 20 to 25 parts of camptotheca acuminate fruit, 15 to 20 parts of peach kernel, 10 to 15 parts of pericarpium trichosanthis, 12 to 15 parts of sedum sarmentosum, 10 to 15 parts of radix cynanchi atrati, 6 to 10 parts of codonopsis pilosula, 5 to 10 parts of semen armeniacae amarae, 6 to 10 parts of adenophora tetraphylla and 4 to 8 parts of liquorice. The traditional Chinese medicine preparation provided by the invention is reasonable in set prescription, monarch, minister, assistant and guide complement each other, and the camptotheca acuminate and peach kernel are used as monarch drugs in the prescription to play the effect of promoting blood circulation to remove blood stasis; the snakegourd peel, the sedum sarmentosum and the cynanchum atratum root are used as ministerial drugs and are matched with the monarch drugs, so that the effects of clearing away heat and toxic materials can be enhanced while the effects of activating blood and removing stasis are enhanced; radix codonopsis, adenophora tetraphylla and semen armeniacae amarae are used as assistants to tonify middle-Jiao and Qi, nourish blood and promote salivation, and licorice is used as a conductant drug to harmonize all the drugs, introduce the drugs into the meridians and eliminate pathogens The whole formula is reasonable in compatibility and has a good treatment effect on sepsis.

The invention relates to the field of plant tissue culture, and particularly discloses an efficient in-vitro accumulation method for camptothecin. The method comprises the following steps: by taking a young stem segment of the camptotheca acuminate as an explant, inducing fixed bud induction, adventitious bud induction, camptothecin in-vitro enrichment I-stage culture, camptothecin in-vitro enrichment II-stage culture, camptothecin in-vitro enrichment III-stage culture, adventitious root camptothecin in-vitro enrichment and the like, inducing accumulation of camptothecin in a whole plant of the camptotheca acuminate. A method for efficient in-vitro accumulation of camptothecin is established. According to the method disclosed by the invention, the camptothecin can be produced under an in-vitro condition, the requirements that the production of the camptothecin is not limited by seasons and the land is saved are met, and the produced camptothecin is natural camptothecin and does not have toxic and side residues.

The invention belongs to the technical field of gene engineering, and discloses a camptotheca acuminate CaTTG1 transcription factor and application thereof. The nucleotide sequence of the camptotheca acuminate CaTTG1 transcription factor is as shown in SEQ ID NO.1, and the amino acid sequence of the polypeptide translated from the camptotheca acuminate CaTTG1 transcription factor is as shown in SEQ ID NO.2. The CaTTG1 can promote the expression of a key enzyme gene in a camptothecin biosynthetic pathway, and HPLC (High Performance Liquid Chromatography) result analysis shows that the maximum camptothecin content in camptothecin leaves is 533 mu g / g and is 1.9 times that of a control group.

This invention provides an application of the camptothecin oxygen methyltransferase CaOMT3 in catalyzing the oxygen methylation modification of hydroxycamptothecin and quercetin. Through transcriptomic and genomic analysis, four oxygen methyltransferases (CaOMT3) were identified. BECAUSE OF Genes were found to be clustered together; further analysis of metabolite and transcriptome co-expression revealed a gene significantly positively correlated with rhamnine accumulation. BECAUSE OF Genes, that is CaOMT3 Biochemical functional verification showed that CaOMT1, CaOMT2, and CaOMT3 can all catalyze the reaction of 9- and 10-hydroxycamptothecin. O Methylation yields the corresponding methoxycamptothecin. When quercetin is used as a substrate, CaOMT1 and CaOMT3 exhibit bifunctional catalytic activity, capable of simultaneously catalyzing the methylation of the 4'- and 7'-hydroxyl sites. O α-methylation yields rhamnosin, tamarindin, and phytoflavin; while CaOMT2 only catalyzes the 4'-hydroxyl site. Enzymatic kinetic analysis showed that CaOMT3's catalytic efficiency for quercetin ( k cat / K (m) is the highest. This invention provides a new enzyme tool and technological foundation for the synthetic biology research and industrial fermentation production of rhamnine and methoxycamptothecin.