13 results about "Cucurbita" patented technology

The invention relates to a diptera adult insecticide, which contains an insecticidal functional component n-butyl alcohol. According to the invention, n-butyl alcohol which has an obvious poisoning effect on the adult cucurbita petiolatus is separated from a metabolite of a bacillus thuringiensis XLB2910 strain, and 2, 3, 5-trimethylpyrazine and benzothiazole which have an obvious poisoning effect on the adult aedes albopictus are also separated from the metabolite of the bacillus thuringiensis XLB2910 strain. Through poisoning concentration analysis of different components and a compound combination experiment, a series of pesticides for adult cucurbita petioles and adult aedes albopictus are formed, the prepared compound pesticide for cucurbita petioles comprises 2.5%-10% of 2, 3, 5-trimethylpyrazine, 2%-10% of benzothiazole and 5%-10% of n-butyl alcohol, and the prepared compound pesticide for aedes albopictus comprises 2.5%-10% of 2, 3, 5-trimethylpyrazine, 2%-10% of benzothiazole and 5%-10% of n-butyl alcohol. The composition comprises 0.5%-3% of 1, 3, 5-trimethylpyrazine, 0.05%-1.5% of benzothiazole and 1%-3% of n-butyl alcohol. The compound insecticide has a good insecticidal effect on cucurbita petiolatus adults and aedes albopictus adults, and is wide in raw material source and environment-friendly.

The invention discloses an application of fat-soluble ring-opened cucurbituril in specific recognition of organic compounds in organic solvents, the fat-soluble ring-opened cucurbituril is composed of glycoluril tetramer and terminal aromatic ring, the terminal aromatic ring is modified by phosphate side chain, and the terminal aromatic ring has good solubility in various organic solvents; according to the invention, the C-shaped adjustable cavity of the fat-soluble ring-opened cucurbituril is matched with the size of a guest molecule, and is matched with hydrogen bonds, pi-pi interaction and the like, so that efficient molecular recognition of organic matters in an organic solvent is realized; the application of the supramolecular macrocyclic main body in organic solvents is expanded by utilizing fat-soluble ring-opening cucurbituril, and the supramolecular macrocyclic main body is expected to be applied to the fields of medicine, chemical industry, environment and the like.

The invention provides a KASP molecular marker related to low-temperature resistance of cucurbit, which is positioned on a cucurbit CM016485.1 chromosome, and is a C-to-T mutation of the 14332833th nucleotide of the cucurbit CM016485.1 chromosome; the KASP primer group for amplifying the molecular marker comprises a forward primer F1, a forward primer F2 and a reverse primer R, and the sequences of the forward primer F1, the forward primer F2 and the reverse primer R are respectively shown as SEQ ID NO.1-3. The invention further provides a KASP primer group related to the low-temperature resistance of the calabash and application of the KASP molecular marker and the primer group. Meanwhile, the invention also provides a method for identifying the low-temperature tolerance of the calabash. The KASP molecular marker and the corresponding primer developed by the invention are used for low-temperature resistance identification and variety breeding of the cucurbit, can effectively solve the problems of long period, high cost and low accuracy of low-temperature resistance identification of the existing cucurbit, and fill the technical blank of molecular marker-assisted breeding in the field.

The application provides a cucurbita wet tolerance related KASP molecular marker, which is located on a cucurbita chromosome 8, and a T to C mutation occurs at the 819th nucleotide of a gene Lsi08G001510 of the cucurbita chromosome 8; a KASP primer set for amplifying the molecular marker comprises a forward primer F1, a forward primer F2 and a reverse primer R, and the nucleotide sequences are shown as SEQ ID NO. 1-3. The application also provides a cucurbita wet tolerance related KASP primer set, and application of the KASP molecular marker and the primer set in cucurbita wet tolerance identification and variety breeding. Meanwhile, the application also provides a cucurbita wet tolerance identification method. The KASP molecular marker and the corresponding KASP primer developed by the application are used for cucurbita wet tolerance identification and variety breeding, can significantly improve the breeding efficiency and accuracy, and have the characteristics of no pollution and low cost.

The present invention provides a novel squash plant having an especially small seed cavity, and a method for producing the squash plant. The present invention provides a method for producing a small seed cavity squash plant that includes developing, from the progeny of an F1 seed obtained by crossing a Cucurbita maxima and a Cucurbita moschata, a small seed cavity squash plant in which the proportion of the volume of the seed cavity relative to the fruit volume is 15% or less, and also provides a small seed cavity squash plant that is a small seed cavity Cucurbita maxima plant having, within the nuclear genome, a gene associated with a small seed cavity trait of a plant of the genus Cucurbita, or a hybrid plant obtained using the small seed cavity Cucurbita maxima plant as a parent, or a progeny of either of these plants.

The invention discloses a supramolecular fluorescent probe and a preparation method and application thereof, preparation raw materials comprise pyrene pyridinium salt derivatives and cucurbit [7] uril, an assembly is formed by electrostatic interaction of the pyrene pyridinium salt derivatives and macrocyclic cucurbit [7] uril, and visual detection is carried out by utilizing fluorescence color change generated by damage of heparin to the assembly. The system can directly identify the color change through naked eyes under the high concentration of heparin, and shows a good linear relationship under the low concentration. The probe has high sensitivity, can provide visual color change at 0.1 mu g / mL, and can be evaluated under the condition of no instrument. The supermolecular fluorescent probe disclosed by the invention is simple in synthesis route, can realize visual recognition of heparin response, has remarkable advantages compared with a traditional fluorescent probe, and has a wide biological application prospect.

The invention provides a Lyomyces rivieri G24 strain and application thereof. The preservation number of the G24 strain is GDMCC No: 67314. The invention further provides a preparation method of the Lyomyces rivieri G24 strain. The application of the Leiobotrys rivularis in prevention and treatment of the Orobanche cucurbitae is reported for the first time, and the types of strains for preventing and treating the growth of the Orobanche cucurbitae are enriched. According to the application of the Lelliottia amnigena G24 provided by the invention in prevention and treatment of Orobanche cucurbitae, the inhibition rate of separated strain fermentation liquor on seed germination of Orobanche cucurbitae is as high as 100%, and the Lelliottia amnigena G24 has wide application value in biological prevention and treatment of Orobanche cucurbitae.

The invention belongs to the technical field of cold stamping process machining, and particularly relates to a scalloped segment convex hole device which comprises a base, a fixing seat is connected below the base, and a concave die is connected above the base. The upper portion of the female die is connected with the male die. A through hole is formed in the center of the base, and a circular boss is arranged at the bottom of the through hole; the fixing base is installed in the through hole, the bottom of the fixing base is matched with the shape of the circular boss, and the outer wall of the fixing base is attached to the inner wall of the through hole; a plurality of stepped holes are evenly formed in the periphery of the through hole. The die is simple in structure, and the weight and the size are greatly reduced compared with those of a traditional convex hole die; according to the die, the mounting steps of the upper die are reduced, the mounting and debugging process is safe and fast, and the qualified rate of formed parts cannot be reduced.

The invention discloses health-care tea for prostate and a preparation method thereof. The health-care tea for prostate comprises the following raw material components in parts by weight: 10-20 parts of sculellaria barbata, 10-20 parts of herba violae, 15-25 parts of purslane, 10-20 parts of radix paeoniae rubra, 150-250 parts of poria cocos, 150-250 parts of coix seeds and 8-12 parts of triquetrous tadehagi herb. The peony flower and bud tea is prepared from 80-120 parts of dried peony flower and bud, 100-200 parts of haw slices, 20-40 parts of snow pear freeze-dried powder, 10-20 parts of camellia pollen and 50-100 parts of green tea or oolong tea. Through scientific compatibility, medicinal materials capable of clearing heat and promoting diuresis, promoting blood circulation to remove blood stasis and tonifying spleen and kidney are combined with characteristic plant raw materials, through an optimized preparation process, prostate health is conditioned in a multi-target mode, and meanwhile the prostate health conditioning tea has excellent taste and stability.

This invention discloses a method for preparing a seven-membered cucurbita supramolecular cocrystal solvent and its application. The invention uses a seven-membered cucurbita as a hydrogen bond acceptor and malic acid as a hydrogen bond donor to prepare a seven-membered cucurbita supramolecular cocrystal solvent. This solvent can be used to extract camptothecin from Camptotheca acuminata fruit, and the extracted camptothecin can be determined by HPLC. This seven-membered cucurbita supramolecular cocrystal solvent is a green extraction solvent that does not use organic solvents when extracting active ingredients from plants, while the extracted active ingredients retain their intrinsic activity. The preparation method is simple, the extraction efficiency is high, and efficient, green extraction is achieved.

The present application relates to a kind of HcCYP89A140 gene of snow fungus cytochrome oxidase and its application, belong to the field of biotechnology.The nucleotide sequence of HcCYP89A140 gene of snow fungus cytochrome oxidase is as shown in SEQ ID NO.1.The present application is with 11-carbonyl-cucurbita-5,24-diene-3β,16α,20,23-tetrol as substrate, under the catalysis of HcCYP89A140, C20 position hydroxyl and C24 position hydroxyl condensation dehydration furan, then again under the catalysis of SDR34, C3 position ketonization, generate 16α,23-dihydroxy-gratiogenin-3-one.The present application realizes the heterologous synthesis of 16,23 double hydroxyl gracin, and it is important to ginseng compound biosynthesis mechanism and its derivative compound and high-efficiency heterologous synthesis.

This invention discloses the U6 promoter of *Cucurbita stenoptera* and its application in CRISPR / Cas9 gene editing of *Cucurbita stenoptera*, belonging to the field of molecular biology technology. The *Cucurbita stenoptera* U6 promoter of this invention comprises the U6-1 promoter and the U6-2 promoter. The nucleotide sequence of the U6-1 promoter is shown in SEQ ID NO.9, and the nucleotide sequence of the U6-2 promoter is shown in SEQ ID NO.11. By replacing the Arabidopsis thaliana U6 promoter and 3'-UTR in the vector with the corresponding 3'-UTR of the *Cucurbita stenoptera* U6 promoter and U6-1, a CRISPR / Cas9 gene editing vector is constructed. *Agrobacterium*-mediated transformation of *Cucurbita stenoptera* is then used to achieve the editing of the target gene, providing an effective approach for studying the gene function of *Cucurbita stenoptera* and accelerating the breeding of new varieties.

The present invention provides a squash plant having an especially small seed cavity, and a method for producing the squash plant. The present invention provides a method for producing a small seed cavity squash plant that includes developing, from the progeny of an F1 seed obtained by crossing a Cucurbita maxima and a Cucurbita moschata, a small seed cavity squash plant in which the proportion of the volume of the seed cavity relative to the fruit volume is 15% or less, and also provides a small seed cavity squash plant that is a small seed cavity Cucurbita maxima plant having, within the nuclear genome, a gene associated with a small seed cavity trait of a plant of the genus Cucurbita, or a hybrid plant obtained using the small seed cavity Cucurbita maxima plant as a parent, or a progeny of either of these plants.