54 results about "Cucurbita" patented technology

The invention belongs to the field of molecular biology and provides cucurbita pepo alpha-tubulin (TUA) gene and application thereof as reference gene. A nucleotide sequence of the cucurbita pepo TUAgene is shown as SEQ ID NO.1, and a cucurbita pepo real-time fluorescent quantitative PCR primer designed according to the gene sequence is shown as SEQ ID NO.2 and SEQ ID NO.3. The designed real-timefluorescent quantitative PCR primer disclosed by the invention has the advantages of strong specificity and very high stability, reliability and repeatability. Researches show that the cucurbita pepoalpha-tubulin can be stably expressed in different tissues, in different periods and under varieties of abiotic stress conditions, so that the cucurbita pepo alpha-tubulin is suitable for serving asthe reference gene in cucurbita pepo gene expression researches.

The invention discloses an immune colloidal gold test strip for quickly detecting a cucumber green mottle mosaic virus (CGMMV) and a preparation method of the immune colloidal gold test strip. The preparation method comprises the following steps: dripping a purified CGMMV polyclonal antibody solution on a detection line of a nitrocellulose membrane; dripping a goat anti-rabbit polyclonal antibody on a quality control line of the nitrocellulose membrane; sequentially attaching a sample pad made of water-absorbing filter paper, a CGMMV immune colloidal gold pad, the nitrocellulose membrane and water-absorbing paper to a polyvinyl chloride (PVC) adhesive plate with an adhesive sticker so as to prepare the test strip. The test strip can be used for detecting the CGMMV in a cucurbitaceous plant and is applicable to detection and diagnosis of a viral disease caused by the CGMMV. The method is simple and quick. The test strip is low in cost. A result can be obtained within 5 to 10 minutes and can be visually and directly observed by naked eyes. The test strip is applicable to quick and extensive screening and detection of diseases through basic agricultural departments at all levels, farmers and entry-exit inspection departments, facilitates timely and effective control and elimination of an epidemic situation, and has a wide application prospect.

The invention discloses a method for identifying authenticity of a cucurbita pepo variety. The method provided by the invention can be used for carrying out early identification on the cucurbita pepovariety in a seed or seedling stage, guarantees the authenticity of the cucurbita pepo variety, practically protects the rights and interests of producers and breeders, and provides a technical support for cucurbita pepo germplasm resources and novel variety protection. The method provided by the invention can be used for identifying an unknown cucurbita pepo variety, and also can be used for identifying the authenticity of a known variety. The method provided by the invention has the advantages of high throughput, accuracy, low cost, simplicity in operation, saving of manpower and material resources and the like, and has extremely wide application prospect.

The present invention discloses a full lotus enzyme which consists of the following raw materials: lotus roots, lotus seeds, nodus nelumbinis rhizomatis, lotus leaves, lotus flowers, lotus stamen, red dates, cucurbita kangua, thladiantha dubia fruits, oligofructose, Angel yeast, glutathione, sucrose, limonene, enzyme mixture, malic acid and water. The provided full lotus enzyme is simple in technology, easy for operation, sweet and sour in taste, and comprehensive in nutrition and strong in functionality. The edible materials are fermented to increase flavoring materials. The full lotus enzyme is sweet, sour and palatable, increases small molecule nutritional factors, is easy for digestion and absorption, promotes intestinal function, and cleans toxins. The full lotus edible ingredients and Chinese herbal medicines are subjected to a scientific compatibility and the full louts enzyme is rich in healing elements, can accelerate healing, promote intestinal function, stop bleeding and relieve bruises, prevent bacteria and inflammation, enhance immunity, prevent cardiovascular and cerebrovascular diseases, beautify features and protect skin, and prevent against aging. Each part of the organs of the lotus roots is fully taken advantage of, which can change waste into treasure, save costs, improve the utilization of the lotus roots, increase the deep processing pathway of the lotus roots, and increase farmers' income.

The invention discloses a high-germination-rate treatment method for accelerating germination of cucurbita kangua li. The method comprises the following steps: firstly, drying seeds of cucurbita kangua li with microwave to remove germs and bacteria on the peels; secondly, immersing the seeds with clear water, and adding 0.02 part of 6-benzylamino adenine into a mixed liquid of 8 percent of sodium bicarbonate solution and 0.05 percent of triacontanol suspension to immerse and loosen the seed shells so as to achieve the effect of early maturity and yield increase; and thirdly, mixing mixture of straw ash, sawdust and tangerine peel powder with the seeds of cucurbita kangua li in a ratio of 1:1 for accelerating germination, irradiating with gamma ray after three days and 96 percent of the seeds of cucurbita kangua li are germinated to kill bacteria and viruses which breed in the germination accelerating process. The seeds can be used for directly seeding or seedling. The method can be used for reducing disease-carrying bacteria of the seeds, enhancing the resistance of seeds in the seeding stage, increasing the germination rate, promoting seedling development and robust growth of cucurbita kangua li and preventing loss of peasants due to the fact that direct seeding causes seed decaying, no germination and seeding-stage delay.

The invention relates to a process for preparation of a hepatoprotective medicament which is prepared from an extract of cucurbitaceous momordica grosvenori and characterized in that momordica grosvenori is firstly disintegrated, heated and extracted through water, and the extracting liquid is separated, concentrated and dried through macroreticular resin column. Purification extracted from momordica grosvenori is prepared into granules, tablets, capsules, oral liquid, dropping pills or syrup, and the medicaments have protective effect to CCI4 chronic liver injury of rats and can decrease activity of ALT and AST in serum and increase TP and ALB, and activity of SOD and GSH-px of liver tissue, reduce MDA content of liver tissue and improve pathologic change of liver tissue.

The invention belongs to the technical field of pesticides, and relates to a bactericidal composition containing dikestrobin and pyrazole indanyl formamide. The bactericidal composition includes active ingredients and auxiliary agents; Pyrazole indanyl formamide and the weight ratio of dikestrobin to pyrazole indanyl formamide is 1:30-30:1. The compound fungicide with dikestrobin and pyrazole indanyl formamide compound as active ingredients has obvious synergistic effect, delays the occurrence of drug resistance in key diseases, and reduces production cost and use cost, and can be used For the control of resistant diseases, it is mainly used to control wheat powdery mildew, rice blast, rice smut, melon powdery mildew, tomato powdery mildew, apple rust, watermelon anthracnose, flower powdery mildew, cucumber downy mildew, grape frost Mildew, scab, anthracnose, spotted leaf spot, etc. also have very good control effects, which are of great significance to the comprehensive control of common diseases.

The invention discloses a method for identifying gynostemma pentaphylla and making a distinction between gynostemma pentaphylla and cayratia japonica at deoxyribonucleic acid (DNA) level. The method is characterized by designing gynostemma pentaphylla SCAR marked specific primer pair WJS-T1 and WJS-T2 by using the result of medicinal plant cucurbitaceae gynostemma pentaphylla DNA characteristic segment SCAR marked clone sequencing, designing internal reference primer pair WHL-18S-C1 and WHL-18S-C2 according to a cucurbitaceae gynostemma pentaphylla 18S rRNA conserved region, forming a polymerase chain reaction (PCR) composite system 1, and amplifying the cucurbitaceae gynostemma pentaphylla DNA by using a touchdown PCR program to obtain two zones, namely a 359bp zone and a 293bp zone; anddesigning the internal reference primer pair WPT-18S-C1 and WPT-18S-C2 according to a vitaceae 18S rRNA conserved region, combining the internal reference primer pair WPT-18S-C1 and WPT-18S-C2 and the gynostemma pentaphylla SCAR marked specific primer pair WJS-T1 and WJS-T2 to form a PCR composite system 2, and amplifying a vitaceae cayratia japonica DNA by using the touchdown PCR program to obtain an 18S rRNA 177bp zone. Therefore, the DNA level of medicinal plant cucurbitaceae gynostemma pentaphylla is identified, and the distinction between the gynostemma pentaphylla and the vitaceae cayratia japonica which is easy to confound with the cucurbitaceae gynostemma pentaphylla in shape is made.

The invention discloses a cucurbita insecticide and a preparation method thereof. The cucurbita insecticide comprises the following compositions in parts by mass: 20-30 parts of pericarpium granati, 18-20 parts of herba houttuyniae, 50-60 parts of cucumber vine, 8-10 parts of leaf of balsampear, 2-4 parts of herba andrographis, 60-70 parts of leaf of lemon eucalyptus, 50-70 parts of pericarpium zanthoxyli, 6-8 parts of fructus anisi stellati, 20-30 parts of pumpkin leaf, 10-20 parts of a material with a Chinese name of Gudongcao, 10-12 parts of fruit of towel gourd, and 2000-2200 parts of water. The cucurbita pepo insecticide is a plant insecticide, and is low in toxicity to mammals, safe to people and livestock during usage, and relatively small in environmental pressure.

The invention provides an oral buccal tablet for quickly eliminating fatigue and strengthening the immunity of organisms, which comprises the following components in portion by weight: 3 to 3,000,000 portions of gynostemma pentaphylla and 1 to 1,000,000 portions of panax quinguefolium. The gynostemma pentaphylla is leaves of sweet gynostemma pentaphylla, curcurbitaceae, originates from e'xi region, Hubei province, and particularly contains trace element selenium. The panax quinguefolium comes from Wisconsin, USA. The oral buccal tablet can quickly eliminate the fatigue, improve the immunity of the organisms, is convenient for carrying and administration at the same time, is suitable for broad crowds, and has no adverse reaction.

The present invention relates to a Cucurbita plant, in particular a squash plant, having wide spectrum resistance to potyvirus such as Zucchini Yellow Mosaic Virus (ZYMV), Watermelon Mosaic Virus (WMV), Papaya Ringspot Virus (PRSV) and Moroccan watermelon mosaic virus (MWMV). Methods of selecting a squash plant having wide spectrum potyvirus resistance by marker assisted breeding are also provided.

The invention belongs to the field of livestock feed, and particularly discloses feed capable of increasing farrowing quantity of sows. The feed comprises, by weight, 34-40 parts of corn flour, 15-18 parts of wheat bran, 15-18 parts of soybean meal, 12-15 parts of bone powder, 5-8 parts of sterile fly maggot, 4-6 parts of barley pest, 15-18 parts of spinach, 8-12 parts of Cucurbita kangua, 3-5 parts of Fructus ziziphi jujubae, 2-4 parts of Japanese ginseng root and 2-4 parts of Stachys oblongifolia. By taking the feed, average farrowing quantity of multiparous landrace sows each birth can reach more than 13.

The invention provides eyesight improving cream, eyesight improving lotion, eyesight improving drink and a preparation method thereof. The eyesight improving drink is prepared from the following raw materials, in parts by mass: 17-31 parts of towel gourd stem juice, 17-22 parts of cucurbita stem juice, 17-23 parts of corn juice, 8-17 parts of semen nelumbinis juice, 5-7 parts of red jujubae juice,9-15 parts of coix chinensis juice, 7-11 parts of juice of Chinese waxgourd, 1-2 parts of anti-tumor ginseng and an appropriate amount of water. Towel gourd, cucurbitae corn, semen nelumbinis, red jujubae, coix chinensis and Chinese waxgourd used in the raw materials are all plants which grow up through a traditional Chinese medicine residue planting method, and the liquid squeezed from the fruits, stems and leaves of the raw materials can have the optimal eyesight improving effect. The traditional Chinese medicine residue planting method comprises the steps that the residue is obtained afternatural and wild traditional Chinese medicines including semen coicis, fructus lycii, poria cocos, radix codonopsis, radix ginseng, radix polygoni multiflori, bulbus lilii and herba dendrobii are boiled, then the residue ferments in a water pool for 70 hours, and is taken out; after 20 hours, the residue is placed on the seeds of the raw materials with thickness of 1 centimeter, the seeds are covered with soil, after young crops grow up, the fruits and stems of the crops can be squeezed to obtain juice.

The invention relates to a metal-doped porous carbon material containing nitrogen and oxygen and its preparation method and application. It uses cucurbituril as a raw material, and performs metal doping after pretreatment to obtain metal-doped cucurbituril, which is finally carbonized The porous carbon material is obtained by processing. The raw material contains nitrogen and oxygen, and the content is uniform. Based on the spatial structure of cucurbituril, it is doped with metal to obtain uniformly doped cucurbitacin, and then the preparation is completed after one carbonization treatment. . The whole process avoids the addition of templates and activators, and the operation is simple and the preparation cycle is short. The metal-doped porous carbon material containing nitrogen and oxygen obtained by the method of the present invention has uniform nitrogen atoms, oxygen atoms and metal elements, rich and uniform pore size distribution, large specific surface area and pore volume, and excellent electrical properties. performance.

The invention discloses the application of Sphingomonas cucurbitus and its fermentation product in preventing and treating bacterial diseases of rice. Specifically including: the application of Sphingomonas cucurbitus and its fermentation products in the preparation of biological control agents for the prevention and treatment of rice bacterial diseases and the application of Sphingomonas cucurbitus and its fermentation products in the preparation of antibacterial agents Wait. By applying Sphingomonas cucurbitus and its fermentation product in rice planting, the disease development of various pathogenic bacteria infecting rice can be effectively inhibited, and different treatment methods can achieve relatively ideal control effects. At the same time, Sphingomonas cucurbitus and its fermentation products can also promote the growth of uninfected rice.

The invention relates to a planting method of cucurbita pepos. The method comprises the following steps of seedling, soil preparing, fertilizing, planting, fertilizer and water managing, and recovering. According to the planting method of the cucurbita pepos, the weight, the germination rate and the protein content of the cucurbita pepos can be increased, and reduce the residue of pesticides in plants, which are beneficial for people to eat the cucurbita pepos.

The invention relates to an extract of a single traditional Chinese medicine, in particular, the invention relates to an extract of acacia saponin, a preparation method thereof, a pharmaceutical composition, an antitumor application and the like.

The invention relates to the field of biological feed brewing wine, in particular to dry lotus root sweet wine. The dry lotus root sweet wine is mainly prepared from the following raw materials in parts by weight: 40 to 50 parts of sticky rice,30 to 40 parts of dry lotus roots, 16 to 20 parts of pachyrhizus, 13 to 17 parts of purple yam, 3 to 5 parts of dry lotus flowers,5 to 9 parts of coconut pulp, 6 to 10 parts of mulberries, 2 to 4 parts of longan aril, 10 to 15 parts of cucurbita kangua, 5 to 9 parts of ficus tikoua bureau, 2 to 4 parts of distiller's yeast, 1 to 3 parts of fermentation strains and 6 to 10 parts of traditional Chinese medicine additives. The dry lotus root sweet wine has the advantages that the color and luster is purplish red; the mouthfeel is fine, cool, crispy, sweet and fragrant; the fragrance of the dry lotus roots and coconut pulp is realized; the vitamin content is high; the types are rich; the human body immunity can be improved; the alcohol content is low; no side effect exists. The added radix puerariae, dry lotus flowers and mulberries can accelerate the ethanol metabolism and can decompose the acetaldehyde toxicity; the absorption of the intestines and the stomach on the alcohol is inhibited; the metabolism and the discharge of the alcohol in the blood can be promoted; the injury to the human body caused by the alcohol is reduced. The traditional Chinese medicine additives for clearing away heat and toxic materials are added, so that the symptoms such as bitter mouth and dry throat, dizziness and headache after drinking can be relieved.

The invention discloses a cis-double-bond amide compound, the structural formula of which is as follows, a preparation method of the cis-double-bond amide compound of the invention, and an application in preparing anti-rheumatoid arthritis drugs. The present invention discovers for the first time the activity of amide compounds containing cis-type double bonds in inhibiting the proliferation of rheumatoid arthritis synoviocytes, which can be used to prepare anti-rheumatoid arthritis drugs; and through column chromatography, thin layer chromatography, Molecular sieve chromatography successfully extracted the amide compound containing cis-double bonds in Melaleuca officinalis.

The invention relates to a medicament for treating freshwater cucurbita disease, a preparation method and a use method. The raw material is composed of glacial acetic acid, salicylic acid, dried pepper, ginger, sodium chloride, and the balance is water. The preparation method is that glacial acetic acid, salicylic acid and sodium chloride are mixed into water and stirred, and after fully dissolving, glacial acetic acid-salicylic acid-sodium chloride prefabricated water preparation is prepared for standby; The above-mentioned prefabricated water preparation is added to the above-mentioned prefabricated water preparation, and the water preparation obtained by filtration after being placed at room temperature is the medicine. For fish infected with freshwater cucurbita disease that can still eat normally, spray this agent on the fish when feeding the bait every day. Spray the agent evenly; a flowing pond slowly dispenses the agent from the mouth of the water. The medicament has good therapeutic effect on freshwater cucurbita disease, low cost, no toxic and side effects to the applicator during the splashing period of the medicament, and is environmentally friendly.

The invention provides a method for cultivating ginseng fruit, which relates to the technical field of cultivating new varieties of zucchini. The method for cultivating the ginseng fruit comprises the following steps: soaking the dry seeds of the wild cucurbita zucchini in Changbai Mountain in water until they are white; The above-mentioned dew white seeds are planted at intervals of 80-90 cm on the ridge, covered with soil, and then heat insulating parts are set on each ridge, and the heat insulating parts are gradually removed; when the seedlings grow to the flowering stage, artificial pollination and pruning are carried out, and finally the fruits are harvested, which is Ginseng fruit. The advantage of the present invention is that the yield, oil yield and aroma of Shenji fruit can be effectively improved by screening and cultivating specific seeds, and the total polysaccharide in the prepared fruit powder is 5.65%, the total cucurbitacin is 3.1%, and the gynoside is 5.65%. 0.92%, the content of linoleic acid in fruit oil is as high as 30%, and it contains a variety of antioxidant and anti-aging ingredients.

The invention belongs to the technical field of biochemical analysis, and particularly relates to a 129Xe 'r2 relaxation efficiency switch type' magnetic resonance molecular probe for recognition of lysine decarboxylase. The probe includes cucurbit [6] urea and lysine. The principle of the molecular probe is as follows: a weak gourd [6] urea-binding substrate (lysine) is converted by lysine decarboxylase into a strong gourd [6] urea-binding product (cadaverine). Before an enzymatic reaction, the relaxation efficiency of the molecular probe is relatively high due to the chemical exchange of 129Xe in cucurbita [6] urea and 129Xe in a soluble state. After lysine decarboxylase is added, lysine is gradually converted into cadaverine by lysine decarboxylase, and the cucurbita [6] urea is occupied by cadaverine. Finally, the relaxation efficiency of the molecular probe becomes almost zero. Recognition of lysine decarboxylase is achieved by changing the relaxation efficiency of molecular probes.

A method of attracting wildlife is provided using the oil of a cucurbit seed. The cucurbit commonly used is pumpkin but can include other cucurbits, such as watermelon, cantaloupe, cucumbers, and squash. The oil is extracted from the seeds and screened. The extracted oil is later dispensed or dispersed at a location where the presence is desired but located outside of the physical reach of wildlife. The oil can be applied to mask the scent of human presence or human activity. The oil draws in wildlife from neighboring areas that are not accessible to hunters or enthusiasts, such as swamps, thickets, or areas with heavy brush. The oil may be used on fishing lures or bait to attract marine wildlife.

The invention belongs to the field of plant molecular biology and plant genetic engineering, and discloses the application of the N subunit protein CsPSI-N of the I reaction center of the cucumber photosynthetic system in the resistance to melon blight. The present invention discovers for the first time that the cucumber photosynthetic system I reaction center N subunit protein CsPSI-N has resistance to cucurbit blight: inoculate Phytophthora cucurbita on the cotyledons of plants overexpressing the cucumber photosynthetic system I reaction center N subunit protein CsPSI-N , the lesion area was significantly reduced, while the cotyledons of plants in which the N subunit protein CsPSI-N of the cucumber photosystem I reaction center was silenced were inoculated with Phytophthora cucurbita, and the lesion area increased significantly; it can be seen that the cucumber photosystem I reaction center N The subunit protein CsPSI-N plays a very important role in plant resistance to melon blight, and can be used for melon blight resistance, preparation of melon blight-resistant products, and cultivation of melon blight-resistant transgenic plants, and has broad application prospects.

The invention discloses a preparation method of a composition containing spirulina polysaccharide extract. The raw materials of phase A are heated and dissolved, and the raw materials of phase B are heated and melted, then added to phase A, stirred while adding, homogenized, and then sequentially during the stirring process. Add the raw materials of phase C, homogenize, stir, and get it. Phase A includes: caprylic triglyceride, CreamMaker‑Blead, GelmakerEMU, vitamin E acetate; or includes: jojoba oil, CreamMaker‑Blead, avocado oil , cetearyl alcohol, vitamin E acetate, GelmakerEMU; Phase B includes: glycerin, sodium hyaluronate, deionized water; or includes: glycerin, sodium hyaluronate, sodium pyrrolidone carboxylate, 1,3-butanedi Alcohol, deionized water; phase C includes: spirulina extract, Budisma japonica extract, grape seed extract, spring elm extract, asiaticoside extract, benzyl alcohol‑DHA, essence. The product of the invention has good heat resistance, cold resistance and other stable characteristics, has good scavenging effect on DPPH and·OH, and has good moisture retention.

The present invention relates to sunscreen cosmetic composition, more specifically, the present invention relates to a kind of biological composition and its application that replaces ultraviolet absorber, and the raw material of described biological composition at least includes Hydrangea extract, Budhimonica extract, salt Dunaliella Algae Extract. The biological composition that replaces ultraviolet absorbers provided by the present invention has high sun protection, safety and stability through the compounding of various components, can replace sunscreen ingredients such as conventional ultraviolet absorbers, and has the effect of reducing redness and brightening the skin.