8 results about "Cucurbitacin" patented technology

The application provides application of a cucumber CsNPF1 gene in regulation of parthenocarpy and fruit bitterness, research finds that the content of auxin in the fruit of a CsNPF1 gene knockout mutant cucumber is significantly lower than that of a wild type, and it is further proved that CsNPF1 promotes the synthesis of auxin in ovules and the formation of parthenocarpic fruits by directly combining with the CsYUC4 promoter sequence and activating the expression thereof; meanwhile, the content of cucurbitacin CuC in the fruit of the mutant cucumber is significantly higher than that of the corresponding wild type, and the expression of a key gene Bitterfruit (Bt) for controlling the CuC content of the cucumber fruit is significantly up-regulated, indicating that CsNPF1 reduces the bitterness of the fruit and improves the quality of the fruit by directly combining with the Bt promoter sequence and inhibiting the expression thereof. The application is conducive to developing a new cucumber variety with high yield and low bitterness of fruit, provides a new direction and important theoretical basis and practical guidance for cucumber molecular breeding, and has a good agricultural application prospect.

The present invention discloses a cucurbitacin-based solid fluorescent material and its preparation and application. The cucurbitacin-based solid fluorescent material is self-assembled in a hydrochloric acid solution using an eight-membered cucurbitacin and trisodium 8-hydroxypyrene-1,3,6-trisulfonate as raw materials. The cucurbitacin-based solid fluorescent material of the present invention is a novel fluorescent material that can be used for visualizing latent fingerprints. The cucurbitacin-based solid fluorescent material of the present invention has the characteristics of high contrast, high sensitivity, good selectivity, non-toxicity, simple preparation and application methods, and low cost.

The invention relates to application of cucurbitic acid as a marker in preparation of a product for identifying or diagnosing adrenocortical carcinoma. The application finds that the cucurbitic acid is closely related to the occurrence of the adrenal cortical carcinoma, so that the application of the cucurbitic acid as the marker to the preparation of related products for identifying, diagnosing and screening the adrenal cortical carcinoma is proposed for the first time. When the compound is used as the marker for predicting the occurrence of the adrenal cortical carcinoma, the early morbidity of the adrenal cortical carcinoma can be accurately and sensitively predicted and identified, so that the technical difficulty of diagnosing the adrenal cortical carcinoma is reduced, and a brand new objective index is provided for identification, diagnosis, screening or drug curative effect evaluation of the adrenal cortical carcinoma.

The present invention provides a benzene-cucurbit ring compound, which belongs to the technical field of macrocyclic compounds. The benzene-cucurbit ring compound is a cage-shaped compound formed by two benzene ring monomers and four glycoside urea monomers connected by 12 methylene-CH2-bridges, and the general chemical formula is C 40 H 36 N 16 O8, with a molecular weight of 868.83200, is a white solid soluble in the organic solvents formic acid, acetic acid, and trifluoroacetic acid. The product composition was accurately confirmed by mass spectrometry, nuclear magnetic resonance, and single crystal structure. The present invention also provides a preparation method and application of a benzene-containing cucurbitacin compound.

This invention discloses an L-amino acid ligase mutant and its application in the synthesis of L-val-cucurbitacin. Compared with existing chemical synthesis methods for L-val-cucurbitacin, the L-val-cucurbitacin synthesis method using the L-amino acid ligase mutant of this invention has convenient raw material sources, simple operation, is green and environmentally friendly, and is easy to implement industrially.

The invention relates to the technical field of traditional Chinese medicines, and provides a traditional Chinese medicine effective part composition for treating hyperplasia of mammary glands, a traditional Chinese medicine preparation and application. Comprising the following components in parts by weight: 10-25 parts of a centipede rhizome total saponin extract, 10-25 parts of a cucurbit rhizome total terpene extract, 10-25 parts of a star-flower root total terpene extract, 5-10 parts of a radix paeoniae alba total terpene extract, 5-15 parts of a platycodon grandiflorum total flavone extract, 5-10 parts of a paederia scandens total terpene extract, 5-10 parts of a gentiana apiata total flavone extract, 5-10 parts of a bighead atractylodes rhizome total terpene extract and 5-10 parts of a fructus aurantii total flavone extract. 5-10 parts of a common cnidium fruit total coumarin extract; the traditional Chinese medicine preparation comprises the composition and auxiliary materials. The invention also discloses application of the composition in preparation of medicines for treating hyperplasia of mammary glands and breast nodules. The traditional Chinese medicine composition directly acts on a focus part according to a treatment principle of activating blood, soothing the liver, removing stasis and regulating endocrine, and is used for treating hyperplasia of mammary glands and breast nodules in a multi-way synergistic manner.

The invention belongs to the technical field of industrial fuel oil preparation, and particularly relates to a method for extracting industrial fuel oil from waste edible oil, which comprises the following steps: firstly, synthesizing a cesium phosphotungstate-cucurbituril clathrate compound catalyst, and obtaining a cesium phosphotungstate-cucurbituril clathrate compound CsPW-CB by using an ultrasonic co-dissolution method; the cesium phosphotungstate-cucurbituril clathrate CsPW-CB catalyst has a good and efficient catalytic effect on an experiment of catalyzing a waste edible oil transesterification reaction to prepare biodiesel; the maximum conversion rate of 95.1% is obtained through two experimental methods, namely a single factor method and a response surface optimization method; the reaction conditions are as follows: the dosage of the catalyst is 2wt%, the alcohol-oil ratio is 11: 1, the reaction time is 150 minutes, and the temperature is 70 DEG C; compared with the prior art, the catalyst with lower price can be self-synthesized, the production time is shortened, the production cost is reduced, and the production efficiency is improved.

The present application relates to the use of cucurbitacin as a marker in the preparation of products for identifying or diagnosing adrenocortical carcinoma. The present application finds that cucurbitacin is closely related to the occurrence of adrenocortical carcinoma, and therefore first proposes to use cucurbitacin as a marker in the preparation of related products for identifying, diagnosing, and screening adrenocortical carcinoma. By using this compound as a marker for predicting the occurrence of adrenocortical carcinoma, the early onset of adrenocortical carcinoma can be accurately and sensitively predicted and identified, thereby reducing the technical difficulty of diagnosing adrenocortical carcinoma, and further providing a brand-new objective index for the identification, diagnosis, screening, or drug efficacy evaluation of adrenocortical carcinoma.