7 results about "Parthenocarpy" patented technology

The application provides application of a cucumber CsNPF1 gene in regulation of parthenocarpy and fruit bitterness, research finds that the content of auxin in the fruit of a CsNPF1 gene knockout mutant cucumber is significantly lower than that of a wild type, and it is further proved that CsNPF1 promotes the synthesis of auxin in ovules and the formation of parthenocarpic fruits by directly combining with the CsYUC4 promoter sequence and activating the expression thereof; meanwhile, the content of cucurbitacin CuC in the fruit of the mutant cucumber is significantly higher than that of the corresponding wild type, and the expression of a key gene Bitterfruit (Bt) for controlling the CuC content of the cucumber fruit is significantly up-regulated, indicating that CsNPF1 reduces the bitterness of the fruit and improves the quality of the fruit by directly combining with the Bt promoter sequence and inhibiting the expression thereof. The application is conducive to developing a new cucumber variety with high yield and low bitterness of fruit, provides a new direction and important theoretical basis and practical guidance for cucumber molecular breeding, and has a good agricultural application prospect.

A Solanaceous plant exhibiting a parthenocarpy and down-regulation in activity or expression of MBP3 and at least one of AGL6 and MBP22, as compared to a control plant, wherein the control plant is of the same genetic background and developmental stage. Also provided are methods of producing the plant and uses thereof.

To identify a novel gene involved in parthenocarpy and to provide a use for this gene. [Solution] This parthenocarpy-regulating gene contains a polynucleotide that (1) encodes the amino acid sequence of SEQ ID NO: 1, (2) encodes an amino acid sequence that has 75% or more sequence identity with the amino acid sequence of SEQ ID NO: 1, (3) encodes an amino acid sequence obtained by substituting, etc., 1-98 amino acids in the amino acid sequence of SEQ ID NO: 1, or (4) hybridizes to a polynucleotide comprising a sequence complementary to a polynucleotide encoding the amino acid sequence of SEQ ID NO: 1 under rigorous conditions.

The invention discloses a pollen suspension for improving the fruit shape and quality of a perfume pomelo as well as a preparation method and application of the pollen suspension. The pollen suspension is obtained by mixing a nutrient solution and Majia pomelo pollen; the nutrient solution is prepared from the following raw materials in parts by weight: 8 to 12 parts of xanthan gum, 5200 to 7800 parts of cane sugar, 36 to 54 parts of calcium gluconate, 4 to 6 parts of boric acid and 40000 to 60000 parts of water; the activity of the Majia pomelo pollen is not lower than 70%; the solid-to-liquid ratio of the Majia pomelo pollen to the nutrient solution is (0.2-0.6) g: 1L; the method can be used for improving the fruit shape and quality of the perfume pomelo, and compared with single-sexual fruiting in a natural state, the method can improve the fruit quality; the malformed fruit rate and the peel thickness of the perfume pomelo are reduced, the edible rate of fruits is improved, and the content of soluble solids in pulp is increased; compared with a traditional cross pollination mode, the method has the advantages that the seed characteristics can be optimized, the seed state of fruits can be obviously changed, and the industrial problem that quality improvement is accompanied by grain increase is solved; the pollination operation efficiency can be improved, and manual labor investment is saved.

The invention provides a gene CsDELLA4 for regulating and controlling parthenocarpy of cucumbers and application of the gene CsDELLA4, a novel gene CsDELLA4 for improving parthenocarpy capacity of cucumbers is identified, and the nucleotide sequence of the gene is as shown in SEQ ID NO. 1. The mutant is obtained through a CRISPR / Cas9 gene editing technology, the parthenocarpy ability of the mutant is enhanced, and it is indicated that the gene CsDELLA4 has a negative regulation effect in the cucumber parthenocarpy process. A new gene resource is provided for genetic improvement of cucumber parthenocarpy molecules, development of new high-yield cucumber varieties is promoted, and the cucumber parthenocarpy gene has a good agricultural application prospect.

The invention discloses a multiple-effect gene CsSUS3 for regulating and controlling cucumber characters and application of the multiple-effect gene CsSUS3, a novel multiple-effect gene CsSUS3 for regulating and controlling parthenocarpy, fruit development and photosynthesis of cucumbers is identified, and the nucleotide sequence of the CsSUS3 gene is as shown in SEQ ID NO. 1. According to the invention, a CsSUS3 gene is knocked out by constructing a CRISPR / Cas9 gene editing vector, so that a function deletion mutant is obtained. Compared with a wild type, the mutant has the advantages that the unisexual maturing rate is remarkably reduced, but the fruit length is remarkably increased, the leaf color is deepened, and the photosynthetic pigment content is increased. The invention discloses a one-cause multiple-effect regulation function of the CsSUS3 gene, and provides a key target gene and a core technical tool for creating a new cucumber germplasm with high fruit yield and high photosynthetic efficiency.

The application discloses a gene CsIAA4 for improving cucumber parthenocarpy and application thereof, identifies a new cucumber parthenocarpic regulation gene CsIAA4, and the nucleotide sequence is as shown in SEQ ID NO. 1. The parthenocarpic capacity of the gene editing mutant of the application is weakened, and the parthenocarpic capacity of the overexpression mutant is significantly enhanced. The gene CsIAA4 of the application can be used for guiding the breeding and improvement of strong parthenocarpic germplasm resources of cucumber, and provides technical support for the breeding of cucumber parthenocarpic varieties. The application jointly explores the application of CsIAA4 in cucumber parthenocarpy through two kinds of transgenic technologies, provides new gene resources for the molecular genetic improvement of cucumber parthenocarpy, and provides the gene CsIAA4 for cucumber parthenocarpy, which has important application value in the research of improving the parthenocarpic capacity of cucumber.