15 results about "Parthenocarpy" patented technology

The application discloses a method for improving the parthenocarpy rate and / or yield of tomatoes, comprising improving the parthenocarpy rate and / or yield of tomatoes by inhibiting or reducing the expression amount of a gene encoding a TSP4.2 protein in the genome of tomatoes. The TSP4.2 protein can be specifically the protein of A1), A2) or A3) as follows: A1) a protein with an amino acid sequence of SEQ ID No. 2 in the sequence listing; A2) a protein obtained by substitution, deletion and / or addition of one or more amino acid residues in the protein of A1), the protein having more than 90% identity with the protein shown in A1) and having the same activity; and A3) a fusion protein obtained by connecting a protein tag to the N terminal and / or C terminal of the protein of A1) or A2). The TSP4.2 protein and related biological materials can be used for regulating the parthenocarpy rate of tomatoes.

The present disclosure relates to a gene that controls parthenocarpy and uses thereof. Specifically, according to the disclosure, when the SlTPP4 gene in a plant is knocked down / knocked out, the plant may form parthenocarpic fruits. When the expression of the SlTPP4 gene g of the present disclosure is inhibited to induce parthenocarpy, the fruit formation rate is 90%, and the parthenocarpic plant exhibits facultative parthenocarpy, and thus may also form normal seeded fruits when pollination occurs. In addition, fruiting occurs well even in a high temperature stress environment.

The application provides application of a cucumber CsNPF1 gene in regulation of parthenocarpy and fruit bitterness, research finds that the content of auxin in the fruit of a CsNPF1 gene knockout mutant cucumber is significantly lower than that of a wild type, and it is further proved that CsNPF1 promotes the synthesis of auxin in ovules and the formation of parthenocarpic fruits by directly combining with the CsYUC4 promoter sequence and activating the expression thereof; meanwhile, the content of cucurbitacin CuC in the fruit of the mutant cucumber is significantly higher than that of the corresponding wild type, and the expression of a key gene Bitterfruit (Bt) for controlling the CuC content of the cucumber fruit is significantly up-regulated, indicating that CsNPF1 reduces the bitterness of the fruit and improves the quality of the fruit by directly combining with the Bt promoter sequence and inhibiting the expression thereof. The application is conducive to developing a new cucumber variety with high yield and low bitterness of fruit, provides a new direction and important theoretical basis and practical guidance for cucumber molecular breeding, and has a good agricultural application prospect.

The present application relates to the field of agricultural technology, and proposes a method for parthenocarpy of grape, comprising the following steps: S1, pinching the new shoots 4-5 days before flowering of grape; S2, cutting the buds 1-2 days after flowering; S3, performing non-nuclear treatment 1-2 days after full flowering; S4, performing fruit grain preservation and fruit enlargement treatment 7-10 days after fruit setting; and S5, applying compound fertilizer. Through the above technical scheme, the problems of low non-nuclear rate of sunlight rose and difficult production in large garden in the related art are solved.

A Solanaceous plant exhibiting a parthenocarpy and down-regulation in activity or expression of MBP3 and at least one of AGL6 and MBP22, as compared to a control plant, wherein the control plant is of the same genetic background and developmental stage. Also provided are methods of producing the plant and uses thereof.

The invention provides a fruit setting agent and a preparation method and application thereof, and belongs to the technical field of plant planting. The invention provides a fruit setting agent. The fruit setting agent comprises gibberellin, boron and chloromethylsilazane. The gibberellin can realize parthenocarpy, the boron has the effects of improving the fruit setting rate and the fruit expansion, and the chloromethylsilatrane has the effects of increasing mitosis, promoting fruit expansion and improving the yield. According to the fruit setting agent, the fruit setting rate of blueberries is increased to 95%-98% or above, the fruit setting rate and yield are guaranteed, and the fruit quality, taste, hardness and storage resistance are also remarkably improved; when the fruit setting agent is sprayed in the flowering period, bee pollination is not affected, fruits sprayed with the fruit setting agent in the same period can expand at the same time, batch maturation of blueberry fruits is achieved to the maximum extent, and fruit bunching harvesting can be achieved. The fruit setting agent provided by the invention provides a technical guarantee for the production targets of high yield, stable yield and high-quality fruits of blueberries.

To identify a novel gene involved in parthenocarpy and to provide a use for this gene. [Solution] This parthenocarpy-regulating gene contains a polynucleotide that (1) encodes the amino acid sequence of SEQ ID NO: 1, (2) encodes an amino acid sequence that has 75% or more sequence identity with the amino acid sequence of SEQ ID NO: 1, (3) encodes an amino acid sequence obtained by substituting, etc., 1-98 amino acids in the amino acid sequence of SEQ ID NO: 1, or (4) hybridizes to a polynucleotide comprising a sequence complementary to a polynucleotide encoding the amino acid sequence of SEQ ID NO: 1 under rigorous conditions.

The invention discloses a pollen suspension for improving the fruit shape and quality of a perfume pomelo as well as a preparation method and application of the pollen suspension. The pollen suspension is obtained by mixing a nutrient solution and Majia pomelo pollen; the nutrient solution is prepared from the following raw materials in parts by weight: 8 to 12 parts of xanthan gum, 5200 to 7800 parts of cane sugar, 36 to 54 parts of calcium gluconate, 4 to 6 parts of boric acid and 40000 to 60000 parts of water; the activity of the Majia pomelo pollen is not lower than 70%; the solid-to-liquid ratio of the Majia pomelo pollen to the nutrient solution is (0.2-0.6) g: 1L; the method can be used for improving the fruit shape and quality of the perfume pomelo, and compared with single-sexual fruiting in a natural state, the method can improve the fruit quality; the malformed fruit rate and the peel thickness of the perfume pomelo are reduced, the edible rate of fruits is improved, and the content of soluble solids in pulp is increased; compared with a traditional cross pollination mode, the method has the advantages that the seed characteristics can be optimized, the seed state of fruits can be obviously changed, and the industrial problem that quality improvement is accompanied by grain increase is solved; the pollination operation efficiency can be improved, and manual labor investment is saved.

This invention discloses a gene SlLHP1 that regulates parthenocarpy in tomatoes, its encoded protein, and its applications. The sequence of the SlLHP1 gene is shown in SEQ ID NO.1. This invention identifies a novel gene SlLHP1 that regulates parthenocarpy in tomatoes. Editing this gene using CRISPR / Cas9 increases the tomato fruit set rate, indicating that the SlLHP1 gene plays a regulatory role in tomato parthenocarpy. Therefore, this invention provides a new gene resource and method for obtaining the parthenocarpy trait in tomatoes, and this method has good application prospects in tomato parthenocarpy breeding.

The invention provides a gene CsDELLA4 for regulating and controlling parthenocarpy of cucumbers and application of the gene CsDELLA4, a novel gene CsDELLA4 for improving parthenocarpy capacity of cucumbers is identified, and the nucleotide sequence of the gene is as shown in SEQ ID NO. 1. The mutant is obtained through a CRISPR / Cas9 gene editing technology, the parthenocarpy ability of the mutant is enhanced, and it is indicated that the gene CsDELLA4 has a negative regulation effect in the cucumber parthenocarpy process. A new gene resource is provided for genetic improvement of cucumber parthenocarpy molecules, development of new high-yield cucumber varieties is promoted, and the cucumber parthenocarpy gene has a good agricultural application prospect.

The invention discloses a multiple-effect gene CsSUS3 for regulating and controlling cucumber characters and application of the multiple-effect gene CsSUS3, a novel multiple-effect gene CsSUS3 for regulating and controlling parthenocarpy, fruit development and photosynthesis of cucumbers is identified, and the nucleotide sequence of the CsSUS3 gene is as shown in SEQ ID NO. 1. According to the invention, a CsSUS3 gene is knocked out by constructing a CRISPR / Cas9 gene editing vector, so that a function deletion mutant is obtained. Compared with a wild type, the mutant has the advantages that the unisexual maturing rate is remarkably reduced, but the fruit length is remarkably increased, the leaf color is deepened, and the photosynthetic pigment content is increased. The invention discloses a one-cause multiple-effect regulation function of the CsSUS3 gene, and provides a key target gene and a core technical tool for creating a new cucumber germplasm with high fruit yield and high photosynthetic efficiency.

The invention relates to an InDel primer pair for identifying parthenocarpy QTL (Quantitative Trait Loci) located on an eggplant chromosome 9 and application of the InDel primer pair. The invention provides a primer pair for identifying parthenocarpy QTL (Quantitative Trait Loci) located on an eggplant chromosome 9, an upstream primer of the InDel primer pair has a nucleotide sequence as shown in SEQ ID NO: 1, and a downstream primer of the InDel primer has a nucleotide sequence as shown in SEQ ID NO: 2. The InDel primer pair disclosed by the invention can be used in the process of breeding parthenocarpic eggplants, so that the breeding efficiency of the parthenocarpic eggplants is improved, and the breeding period is greatly shortened.

The application discloses a gene CsIAA4 for improving cucumber parthenocarpy and application thereof, identifies a new cucumber parthenocarpic regulation gene CsIAA4, and the nucleotide sequence is as shown in SEQ ID NO. 1. The parthenocarpic capacity of the gene editing mutant of the application is weakened, and the parthenocarpic capacity of the overexpression mutant is significantly enhanced. The gene CsIAA4 of the application can be used for guiding the breeding and improvement of strong parthenocarpic germplasm resources of cucumber, and provides technical support for the breeding of cucumber parthenocarpic varieties. The application jointly explores the application of CsIAA4 in cucumber parthenocarpy through two kinds of transgenic technologies, provides new gene resources for the molecular genetic improvement of cucumber parthenocarpy, and provides the gene CsIAA4 for cucumber parthenocarpy, which has important application value in the research of improving the parthenocarpic capacity of cucumber.

The invention relates to the technical field of plant biology, in particular to an expression construct combination product and a method for producing parthenocarpic siraitia grosvenorii. The combination product comprises expression constructs a) and b), the invention relates to a floral organ specific promoter having an iaaM gene and a floral organ specific promoter operably linked to the iaaM gene; b) having an IAA9 gene and a leaf tissue specific promoter operably linked to said IAA9 gene. According to the construction body, the fruit setting rate under the non-pollination condition can be remarkably increased, the siraitia grosvenorii fruits which are complete in structure and free of seeds are formed, the artificial pollination burden and the follow-up mogroside extraction cost are reduced or eliminated, and the siraitia grosvenorii fruits with few seeds or free of seeds can be produced.

The invention relates to the technical field of molecular markers, in particular to a molecular marker related to cucumber parthenocarpy and application of the molecular marker. The base sequence of the InDel molecular marker is as shown in SEQ ID NO.4, wherein the 188th to 199th sites of the SEQ ID NO.4 have insertion deletion variation; the drought cucumbers of which the sequences from the 188th site to the 198th site are TGTTTGTGAG in the SEQ ID NO.4 are parthenocarpy; and the drought cucumbers of which the sequences TGTTTGTGAG at the 188th site to the 198th site in the SEQ ID NO.4 are deleted are non-parthenocarpy. When the molecular marker is applied to breeding, early selection can be realized, the breeding efficiency of parthenocarpy varieties is remarkably improved, the labor cost is reduced, and genetic improvement and industrial development of the drought cucumber varieties special for facilities are promoted.