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Linkage molecular marker of main effect QTL Parth2.1 of cucumber parthenocarpy and application thereof

A technology of parthenocarpy and molecular markers, which is applied in the field of molecular genetic breeding of vegetables, can solve problems such as time-consuming, easily affected by the cultivation environment, and parthenocarpy easily affected by external conditions, so as to achieve high yield, improve breeding efficiency, The effect of good fruit quality

Active Publication Date: 2019-09-03
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the selection of cucumber parthenocarpic materials or varieties mainly relies on phenotypic identification by isolation of bunches of flowers in the field. The efficiency is not high, the breeding is difficult, and the cultivated cucumber varieties have the disadvantages of unstable parthenocarpy and being easily affected by the cultivation environment.

Method used

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  • Linkage molecular marker of main effect QTL Parth2.1 of cucumber parthenocarpy and application thereof
  • Linkage molecular marker of main effect QTL Parth2.1 of cucumber parthenocarpy and application thereof
  • Linkage molecular marker of main effect QTL Parth2.1 of cucumber parthenocarpy and application thereof

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Experimental program
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Effect test

Embodiment 1

[0034] Example 1: Parth2.1-linked molecular marker development and its molecular detection in the F2:4 population

[0035] 1. Fine mapping and peak marker development of parthenocarpy main effect QTL Part2.1

[0036] Using the strong parthenocarpy inbred line 'EC1' as the female parent and the non-parthenocarpic inbred line '8419s-1' as the male parent, an F2 segregation population was constructed, and the preliminary positioning of the main QTL Part2.1 for parthenocarpy was completed (Zhe Wu, Ting Zhang, Lei Li, Jian Xu, Xiaodong Qin, Tinglin Zhang, Li Cui, Qunfeng Lou, Ji Li* and Jinfeng Chen*. Identification of a stable major-effect QTL (Parth 2.1) controlling parthenocarpy in cucumber and associated candidate gene analysis via wholegenome re-sequencing[J].BMC plant biology,2016,16,182), on this basis, the individual plant #68 carrying Parth2.1 and parthenocarpy rate greater than 90% was selected as the donor from the F2 population Parents, the non-parthenocarpic material ...

Embodiment 2

[0041] Embodiment 2: the cultivation and application of the all-female parthenocarpic cucumber variety Parth139, including:

[0042] 1. Parent traits

[0043] (1) All-female, strongly parthenocarpic trait donor parent EC1

[0044] EC1 (Zhe Wu, Ting Zhang, Lei Li, Jian Xu, Xiaodong Qin, Tinglin Zhang, LiCui, Qunfeng Lou, Ji Li and Jinfeng Chen. Identification of a stable major-effect QTL (Parth 2.1) controlling parthenocarpy in cucumber and associated candidate gene analysis via whole genome re-sequencing [J]. BMC plant biology, 2016, 16, 182) is an all-female parthenocarpy European greenhouse cucumber inbred line material. It has strong plant growth, well-developed side branches, main and side branch results, and commercial fruit shape It is short rod-shaped, and the parthenocarpy rate exceeds 90%.

[0045] (2) Weak female, non-parthenocarpic parent 8419

[0046] 8419 (Wu Zhe, Li Lei, Zhang Ting, Zhang Tinglin, Li Ji, & Lou Qunfeng et al. (2015). QTL mapping of cucumber par...

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Abstract

The invention discloses a linkage molecular marker of a main effect QTL Parth2.1 of cucumber parthenocarpy and an application thereof. The linkage molecular marker Indel-16Y-46 of the main effect QTLParth2.1 of cucumber parthenocarpy has an upstream primer of SEQ ID NO.1 and a downstream primer of SEQ ID NO.2. Provided is the application of the molecular marker in molecular marker assisted breeding of a new full-female strong parthenocarpic cucumber variety. The selection efficiency of the molecular marker on a Parth2.1 locus is 100%. Based on the molecular marker, a method for molecular marker assisted breeding of the new full-female strong parthenocarpic cucumber variety is developed. The obtained new full-female strong parthenocarpic cucumber variety is a greenhouse cucumber selfing line variety in Europe, has high yield and good fruit quality, and can be directly used for improving the parthenocarpy and full-female primal parents of other varieties.

Description

technical field [0001] The invention discloses a linkage molecular marker of cucumber parthenocarpy main effect QTL Part2.1 and its application, belongs to the technical field of vegetable molecular genetic breeding, and can be used for cucumber parthenocarpy molecular marker assisted breeding. Background technique [0002] Cucumber is one of the main vegetables cultivated in a large area in my country, and has extremely high social value and economic benefits. Parthenocarpy refers to the phenomenon that the ovary develops into fruit without pollination, fertilization or other stimulation. Cucumber varieties with strong parthenocarpic ability have significant potential to increase yield, generally can increase yield by more than 20%, and parthenocarpic fruits are seedless, less pulp, thick flesh, so that the quality is improved and the commodity is good. Therefore, parthenocarpy is an important economic trait closely related to cucumber yield and quality, and is one of the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11A01H1/02A01H1/04
CPCA01H1/02A01H1/04C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 李季陈劲枫朱拼玉娄群峰钱春桃虞夏清程春燕
Owner NANJING AGRICULTURAL UNIVERSITY
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