Method for rapid in-vitro propagation of Crassulaceae plant

A technology of Crassulaceae and plants, which is applied in the field of rapid in vitro propagation of Crassulaceae plants, can solve the problems of long cycle, easy degradation of traits, and limitations in the research, exploration and utilization of the physiological characteristics of Crassulaceae plants, so as to shorten the growth cycle and improve germination rate, the effect of improving breeding efficiency

Inactive Publication Date: 2015-01-14
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method is directly taken from adult plants, which completely retains the traits of the female parent, and cannot produce new traits and varieties, and the traits are easily degraded.
Traditional vegetative propagation needs to take mature tissues such as leaves or stems on the basis of adult plants for cutting propagation, which takes a long period (Qiu Ninghong et al., Sedum tissue culture and rapid propagation technology. Special Economic Animals and Plants, 2003, 6(12) : 20; Luo Linhui et al., Rapid Propagation of Sedum sedum in Vitro. Special Economic Animals and Plants, 2003, 6(10): 24; Zhao Li, Effects of Different Nutritional Conditions on Callus Induction and Plant Regeneration of Sedum sedum. , 2005, master's thesis of Sichuan Agricultural University), which caused great restrictions on the research on the physiological characteristics of sedum plants and the exploration and utilization of horticultural characteristics.

Method used

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  • Method for rapid in-vitro propagation of Crassulaceae plant

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] (1) Medium preparation

[0064] Prepare plant MS solid medium, add GA 3 (Gibberellin, the final concentration is 5mg / L) and BA (benzylaminoadenine, the final concentration is 0.5mg / L), adjust the pH value to 5.8, high temperature and high pressure sterilization, pour into the 9cm plate for subsequent use.

[0065] Prepare MS medium containing 1mg / L BA (benzylaminoadenine) and 0.1mg / L NAA (naphthaleneacetic acid) at the same time; prepare MS medium containing 0.2mg / L BA (benzylaminoadenine), 0.02mg / L NAA ) and 1mg / L gibberellin GA 3 MS medium.

[0066] (2) Seed disinfection

[0067] Put the Crassulaceae seed Guanghan Palace into a 1.5ml EP tube, add 0.01-0.05% trace potassium permanganate solution (lavender) to soak for 1 hour, rinse and transfer to a 1.5ml EP tube and add 10% sodium hypochlorite solution for treatment 8 minutes, during which the seeds were mixed with the solution for 3-5 times with a vortex shaker, and the solution was sucked out after being placed ...

Embodiment 2

[0083] The steps of the present embodiment are basically the same as in Example 1, except that the red paw seeds are used in the difference.

[0084] The experimental procedure was repeated three times, and a total of 265 regenerated plants of Red Claw were obtained. After testing, the phenotype of the plant was 100% consistent with the phenotype of the parent. The contemporary reproduction factor is 15-20 seedlings / explant and the regeneration frequency is 100%.

[0085] Using conventional methods, it takes about 14 months for red claw to become commercialized large seedlings, but in this example, it only takes 8-10 months to obtain commercialized large seedlings through in vitro rapid propagation, which greatly shortens the time required for cultivation.

Embodiment 3

[0087] The steps of the present embodiment are basically the same as in Example 1, except that sweet-scented osmanthus seeds are used.

[0088] The experimental procedure was repeated three times with a contemporary reproduction factor of 20-25 seedlings / explant and a regeneration frequency of 100%. A total of 279 regenerated plants of Osmanthus osmanthus were obtained. After testing, the phenotype of the plant was 100% consistent with the phenotype of the parent.

[0089] Using conventional methods, it takes about 16 months for Osmanthus osmanthus to become commercialized large seedlings, but in this example, it only takes 8-10 months to obtain commercialized large seedlings through in vitro rapid propagation, which greatly shortens the time required for cultivation.

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Abstract

The present invention discloses a method for rapid in-vitro propagation of a Crassulaceae plant. The method comprises the steps of carrying out aseptic germination of seeds, inducing adventitious bud, and carrying out in-vitro rooting of the adventitious buds. The method allows in-vitro germinated seedlings to normally grow and to be induced within a short period of time to continuously differentiate from original single-seed single seedlings into single-seed multiple seedlings, so the propagation cycle is greatly shortened, and the growth cycle of the differentiated seedlings is greatly shortened. The method provides a stable technical platform for improvement of the breeding efficiency of high-quality varieties of the Crassulaceae plant.

Description

technical field [0001] The invention relates to the field of sedum plant cultivation, in particular to a method for rapid in vitro propagation of sedum plants. Background technique [0002] Crassulaceae plants are a class of succulents that are widely distributed and have high ornamental and medicinal value. Sedum family plants are dicotyledonous plants, there are about 1600 species, distributed all over the world, herb or subshrub, usually succulent. Leaves alternate, opposite or whorled, solitary, mostly sessile, entire, serrated, lobed or pinnatifid, without stipules. The fruit is a follicle, membranous or hairy, often surrounded by the remaining membranous petals, dehiscing; the seeds are small, disc-shaped, often with fleshy endosperm. There are about 25 genera and more than 1,000 species in the world, native to Africa, the Americas and the temperate regions of Europe. [0003] There are about 240 species of 10 genera of Crassulaceae plants in China, and many others ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 朱木兰马俐李胜
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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