197results about How to "Genetic improvement" patented technology

The invention discloses a method for determining optimal SNP quantity as well as performing genome selective breeding on production performance of large yellow croakers through selection markers. The method comprises the following steps: performing phenotype determination and genomic sequencing on the production performance of individuals of a reference group to obtain SNP locus; screening out the qualified SNP locus and supplementing the deleted genotype; dividing the reference group into a training set and a validation set to perform hybridization validation; screening the SNP locus most remarkably associated with the character through single marker analysis, and then calculating GEBV of individuals of the validation set only by using the locus and through a GBLUP method; further obtaining breeding value estimation accuracy under each screening SNP quantity; finally determining the optimal quantity of SNP screening; and according to the optimal quantity, calculating the GEBV by the GBLUP method, further obtaining the breeding value estimation accuracy and performing genome selective breeding according to the value size. By the method, the genomic selection cost on the production performance of the large yellow croakers can be reduced remarkably.

The invention belongs to the technical field of gene engineering, and specifically relates to a set of paddy rice anti-brown-planthopper genes, and coded proteins thereof and an application thereof. The sequences of the paddy rice anti-brown-planthopper genes are represented by SEQ ID No.5-No.8. The sequences of the coded proteins are represented by SEQ ID No.1-No. 4. The paddy insect-resistance-related proteins influence the insect resistance of plant. The increase of the protein-coding gene can lead to insect resistance of susceptible plants, such that anti-brown-planthopper transgenic plants can be cultivated. The gene and the coded protein thereof can be used in plant genetic improvement.

The present invention discloses a method for rapid in-vitro propagation of a Crassulaceae plant. The method comprises the steps of carrying out aseptic germination of seeds, inducing adventitious bud, and carrying out in-vitro rooting of the adventitious buds. The method allows in-vitro germinated seedlings to normally grow and to be induced within a short period of time to continuously differentiate from original single-seed single seedlings into single-seed multiple seedlings, so the propagation cycle is greatly shortened, and the growth cycle of the differentiated seedlings is greatly shortened. The method provides a stable technical platform for improvement of the breeding efficiency of high-quality varieties of the Crassulaceae plant.

The invention relates to cultivation gynogenetic fish, in particular to a method for cultivating gynogenetic carps. The method comprises the following steps of: performing artificial induced spawning on male megalobrama amblycephala and female ordinary carps which serve as male and female parent fish, mixing megalobrama amblycephala sperms inactivated by ultraviolet rays and mature ova of the ordinary carps, activating for 1 to 2 minutes, performing cold shock on the activated ova at the temperature of between 0 and 4 DEG C for 30 to 40 minutes, and incubating embryos obtained by the cold shock at the water temperature of between 20 and 21 DEG C to obtain diploid gynogenetic carps. The gynogenetic carps cultivated by the method have excellent descendant properties, high survival rate and stable inheritance, and have the great significance for the study on biological genetic breeding.

The invention discloses a sequence of rice high-affinity nitrate transport protein gene OsNAR2.1 and application thereof, belonging to the gene engineering field. The invention also discloses a cDNA sequence SEQ ID NO.1 of the rice high-affinity nitrate transport protein gene OsNAR2.1 and a coded mino acid sequence SEQ ID NO.2 thereof. The gene function is firstly reported in rice and expressed in frogspawn heterogenous system to determine that the OsNAR2.1 protein can remarkably promote the transportation of nitrate by the rice; and mRNA expression analysis shows that the gene is subject to the induction of low nitrate nitrogen and the inhibition of ammonium and nitrogen metabolin. The knockout mutant of the gene causes the rice to reduce the absorption of nitrate and have obvious anazotic symptoms, the gene also influences the ripening rate of the rice through the field experiments, and therefore, over expression OsNAR2.1 can improve the absorption of nitrate nitrogen of the rice under low-nitrogen condition and the yield of the rice.

The invention discloses a plant male fertility related protein and a coded gene and application thereof. The protein is of (a) or (b): (a) the protein formed by an amino acid sequence indicated in SEQ ID NO.1; and (b) the protein related to plant male fertility and derivate from the SEQ ID NO.1 by using one or a plurality of amino acid residues to replace and / or delete and / or add in the amino acid sequence 1. The plant male fertility related protein influences the anther cracking process of plants. Plant male fertility can be reduced and maturing rate can be affected by restraining expression of the coded gene of the protein, and accordingly transgenic plants with male anther non-cracking variation and plant male sterile line transgenic plants can be cultured. Male fertile transgenic plants can be cultured by introducing the coded gene of the protein into male sterile plants. The protein and the coded gene of the protein can be applied to plant genetic improvement.

The invention discloses a monopterus albus gene editing method. The monopterus albus gene editing method comprises the following steps: 1, acquiring an artificially inseminated monopterus albus 1-cell stage embryo by establishing an indoor eel whole artificial propagation technology; 2, establishing a transcription activator-like effector nuclease gene editing vector of a target gene, performing in-vitro transcription to synthesize mRNA, and transferring the mRNA into the monopterus albus 1-cell stage fertilization embryo by adopting a microinjection method; 3, screening to obtain a monopterus albus with a mutant target gene through a three-primer detection method, and determining the mutation type of the target gene through sequencing. According to the method, a micromanipulation technology for a monopterus albus embryo is established for the first time, a precise monopterus albus endogenous gene editing technology is established for the first time, and powerful technological means is provided for performing function research on a monopterus albus gene and genetic improvement of monopterus albus breeding variety.

The invention belongs to the field of gene engineering, and relates to a protein OsGPA3 relevant to sorting of plant glutelin, an encoding gene of the protein OsGPA3 and applications of the protein OsGPA3. The protein is a protein (a) consisting of an amino acid sequence shown in a sequence table 1 or a protein (b) which is prepared by replacing and / or removing and / or adding one or several amino acid residues in the amino acid sequence in the sequence table 1 and is relevant to the sorting of the plant glutelin and derivates from the sequence 1. The protein influences the sorting of glutelin precursors in a plant endosperm. The encoding gene of the protein is introduced into a plant in which the glutelin is sorted abnormally, so that a transgenic plant in which the glutelin is sorted normally can be cultured. The protein and the encoding gene thereof can be applied to genetic improvement of plants.

The invention relates to an SNP marker for determining and / or inheriting growth traits of modified pigs. The SNP marker comprises at least one of 14 SNP markers respectively positioned at a No. 16806345 site, a No. 16940077 site, a No. 17473931 site, a No. 17013787 site, a No. 17096014 site, a No. 17007981 site, a No. 16916995 site, a No. 17102194 site, a No. 17114026 site, a No. 16682972 site, a No. 16765047 site, a No. 16788727 site, a No. 16767555 site and a No. 17472686 site on a No. chromosome of a 10.2 edition of international swine genome from the 5' end.

The invention discloses a vegetable glutelin transportation storage related protein OsVps9a as well as a coding gene and application thereof. The protein is selected from (a) or (b) as follows: (a) protein formed by an amino acid sequence shown in SEQ ID NO.1; and (b) protein for replacing and / or deleting and / or adding one or more amino acid residues in the amino acid sequence shown in SEQ ID NO.1, related to the vegetable glutelin transportation storage and derived from the SEQ ID NO.1. Vegetables with normal mature glutelin content can be cultivated by introducing the coding gene of the protein into vegetables with lowered mature glutelin content. The protein and the coding gene can be applied in the genetic improvement of vegetables.

The invention belongs to the technical field of plant genetic engineering and discloses application of a rice phosphate transport protein gene ORYsa;Pht1;4. Root specific expression of the rice phosphate transport protein gene ORYsa;Pht1;4 refers to a root specific expression gene which is discovered in paddy rice for the first time. The gene can be applied to the development of a rice variety which does not contain any genetically modified ingredient in paddy rice particles, can be eaten trustingly and increases the utilization ratio of available phosphorus in soil.

The present invention provides a new strain of bacillus circulans WZ-12 which can degrade methylene chloride and an application of the bacillus circulans WZ-12 for microorganism to decompose and dispose the methylene chloride and preparing for methylene chloride dehalogenase. The bacillus circulans WZ-12 is conserved in the China Center for Typical Culture Collection; the conserving date is 19th, January, 2007; the conserving serial number CCTCC No. is M207006. The beneficial effect of the present invention mainly lies in providing the new strain of the bacillus circulans WZ-12 which can degrade the methylene chloride, the bacillus can degrade the methylene chloride fast under the aerobic condition, which provides important base for researching into the biological degradation mechanism of halohydrocarbon organic pollutant represented by the methylene chloride, and the bacillus can also be applied to a biological disposal technology of organic waste gas directly. Besides, the strain is wild type with very clear genetic background, which is fit for genetic improvement and is hopeful to improve the capability of disposing the organic waste gas greatly.

The invention relates to a tissue culture method of Gentiana dahurica Fisch for rapid in vitro reproduction. The method comprises the following steps: (1) selection and sterilization process of explant, comprising taking the stem tip of seedling of biennial Gentiana dahurica Fisch in a greenhouse as the explant, dipping for sterilization and washing so as to obtain the sterilized explant stem tip; (2) inoculation of explant, comprising inoculating the explant stem tip after sterilization at a callus tissue induction culture medium so as to form callus tissue; (3) proliferation and differentiation culture of the callus tissue, comprising inoculating the callus tissue to a callus tissue proliferation culture medium to culture so as to form single buds of Gentiana dahurica Fisch; (4) induction of root growth, comprising separating the single buds of Gentiana dahurica Fisch and afterwards transplanting into the root growing culture medium to culture so as to form complete regenerated plantlets; (5) sterilization of seedling medium; (6) transplanting of plant, comprising transplanting the complete regenerated plantlets into the seedling substrate subjected to sterilization directly, and acclimating the seedling so as to grow normal Gentiana dahurica Fisch plants. According to the method, the problems of overlong seedling period and low reproduction coefficient of Gentiana dahurica Fisch are overcome, and the method is easy to operate and can be used for rapid factory seedling culture.

The invention relates to the technical field of plant gene engineering, in particular to a method for increasing the grain number per ear and reducing plant height by use of rice SNB genes. The nucleotide sequence of SNB genes is shown as SEQ ID NO:1. Artificially mutant rSNB genes are obtained by changing the binding site of microRNA172 in the SNB genes, the nucleotide sequence of the rSNB genes is shown as SEQ ID NO:2, and the sequence of protein encoded by the rSNB genes is shown as SEQ ID NO:3. The grain number per ear of rice is remarkably increased and the plant height of the rice is notably reduced through overexpression of rSNB, and further studies prove that SNB encoded protein has the activity of a transcription suppressor. The functions of the SNB genes on increasing the grain number per ear of the rice and reducing the plant height of the rice are disclosed, and a novel rice genetic improvement method is also provided.

The invention discloses a method for quickly propagating plukenetia volubilis linneo. The method includes steps of disinfecting the surfaces of explants which are current-year non-lignified branches of the plukenetia volubilis linneo; cultivating and inducing callus tissues of the explants; inducing differentiation and elongation of buds by the aid of the callus tissues; strengthening and rooting seedlings; exercising and transplanting the seedlings and the like to obtain regenerated plants. The method has the advantages that integral procedures can be carried out under controllable conditions without seasonal constraints, the propagation cycle can be shortened to a great extent, the propagation multiples can be increased, the method has important practical significance on meeting requirements on high-quality seedlings of the plukenetia volubilis linneo and accelerating popularization of improved varieties of the plukenetia volubilis linneo, and an efficient and feasible technical means can be provided for future research on genetic modification and the like on the plukenetia volubilis linneo.

The invention relates to a sequence related to red and yellow color properties of citrus peel. The sequence is shown in SEQ ID NO:1, and is positioned at the upstream of a gene for encoding carotenoid splitting dioxygenase. The invention further provides application of the sequence in citrus breeding, and a method for predicting the color of citrus peel or the color of peel of a filial generation of citrus in the breeding process. The method comprises a step of detecting whether the sequence as shown in SEQ ID NO:1 at the upstream of the gene for encoding the carotenoid splitting dioxygenase exists or not, and / or detecting polymorphism of an SNP site in the sequence. The invention further provides a breeding method for changing the color of citrus peel. The sequence has great instruction significances for genetic improvement of appearance colors of citrus, breeding efficiency improvement and breeding cost reduction.

The invention discloses a tissue culture and rapid propagation method of didymostigma obtusum. According to the tissue culture and rapid propagation method of the didymostigma obtusum, leaves of the didymostigma obtusum are utilized as explants to achieve rapid propagation of seedlings of the didymostigma obtusum which belongs to rare ornamental plants through screening and optimization of the formula and culture conditions of culture medium primary generation, transgenerational proliferation, rooting and transplanting and through the steps of primary generation, transgenerational proliferation and rooting culture of adventitious buds and transplanting of test-tube seedlings. Generally only 110 days are required to obtain the seedlings of the didymostigma obtusum and the survival rate of the seedlings of the didymostigma obtusum can reach more than 95%. Accordingly, the tissue culture and rapid propagation method of the didymostigma obtusum has the advantages of being simple, rapid and effective and directly applicable to breeding and protection of the didymostigma obtusum and accordingly laying a foundation for genetic improvement and protection, development and utilization of germplasm resources of the species.

The invention discloses a rice blast resistance gene Pikg, encoding protein and application thereof, and belongs to the field of rice disease resistance breeding. The rice blast resistance gene Pikg comprises Pikg<-1> and Pikg<-2>, and the nucleotide sequence of a coding region and the amino acid sequence of coding protein of the rice blast resistance gene Pikg are respectively shown in a sequencetable. The gene and the protein can be used for improving the resistance of rice plants to magnaporthe griseat.

The invention discloses an angelica tissue culturing and breeding method, which comprises the following steps: adopting angelica root as external growing bulk, sterilizing, grafting to induce callus, breeding, proceeding bud and root induction, forming entire plant sprout, planting the entire sprout in the substrate, adopting MS culture medium as base and 6-furfuryl adenine, 6-benzyl adenine, 2, 4-dichlorophenoxyacetic acid, fruitone, sucrose and active carbon as auxiliary compositions.

The invention discloses a gene OsHsp70cp-2. The gene OsHsp70cp-2 is a DNA (deoxyribonucleic acid) molecule shown in (1) or (2) or (3) or (4), namely (1) a DNA molecule shown in SEQ ID NO.1; (2) a DNAmolecule shown in SEQ ID NO.2; (3) a DNA molecule which is hybridized with a DNA sequence limited in (1) or (2) under the strict condition and is used for encoding protein; (4) a DNA molecule which has 90% of homology or more with the DNA sequence limited in (1) or (2) or (3) and is used for encoding the protein related with synthesis of plant starch. The protein related with plant endosperm flouraffects the development process of plant amyloplast. The abnormal development of the amyloplast in the plant seeds is caused by inhibiting the expression of the protein encoding gene, so as to culture a transgenic plant with endosperm variation and a transgenic plant with abnormal plant amyloplast.

The invention discloses a genetic transformation method of a Chinese fir. The genetic transformation method comprises the steps of: establishment of a transformation receptor, preparation of agrobacterium liquid, infection, bacterium removal culture and screening culture. According to the genetic transformation method of the Chinese fir disclosed by the invention, a Chinese fir suspension cell system is taken as the receptor and a b-glucuronidase (GUS) gene is used as a report gene to implement a genetic transformation through an agrobacterium mediated method, and the plant regeneration is realized through a somatic embryogenesis way, and a stable and efficient Chinese fir genetic transformation system is established. Due to the utilization of the technical system, genetic improvement of the Chinese fir with comparatively low cost can be achieved; and anti-disease, stress-resistant, wood formation and other related genes obtained from a basic and theoretical research are subjected to the genetic transformation so as to obtain a high-quality Chinese fir variety having characteristics of rapid growth, strong anti-disease and stress-resistant performances, and the like.

The invention discloses an Ornithogalum arabicum Linn. tissue culture rapid breeding method. The method comprises selecting leaves of Ornithogalum arabicum Linn. as explants for tissue culture, carrying out leaf surface bulbil induced culture to obtain a seedling, or leaf callus induced culture, adventitious bud induced culture and rooting culture to obtain a seedling, carrying out tissue culture seedling acclimatizating and transplanting after the seedling produces adventitious root, and then carrying out normal culture. The method solves the problem that the underground bulb breeding method has regeneration difficulty and a low seedball propagation coefficient, has the advantages of operation simpleness and high propagation coefficient, is not limited by regions, seasons and climate and realizes industrial large-scale production of high-quality seedlings.

The invention belongs to the field of genetic engineering and relates to plant glutelin transportation and storage related protein OsNHX5 as well as an encoding gene and an application of the protein. The protein is protein shown in (a) or (b), wherein (a) is protein comprising an amino acid sequence shown in SEQ ID NO.1; (b) is plant glutelin transportation and storage related protein derived from SEQ ID NO.1 through substitution and / or deletion and / or addition of one or several amino acid residues of an amino acid sequence in SEQ ID NO.1. The plant glutelin transportation and storage related protein affects transportation and storage of glutelin precursors in plant endosperm. The encoding gene of the protein is introduced to plants which are abnormal in glutelin transportation and storage, and transgenic plants which are normal in glutelin transportation and storage can be cultured. The protein and the encoding gene thereof can be applied to genetic improvement of plants.

The invention discloses a root development-regulating apple polypeptide hormone gene MdCEP7 and the application thereof, and belongs to the technical field of molecular biology. MdCEP7 gene is ectopically expressed in Arabidopsis thaliana by a transgenic technology using a 35S strong promoter driving principle to obtain transgenic Arabidopsis thaliana; overexpression of MdCEP7 in the Arabidopsis thaliana affects synthesis and transportation process of plant root auxin; further phenotypic observation shows that the overexpression of MdCEP7 in the Arabidopsis thaliana significantly inhibits elongation of main roots and the number of lateral roots; the results show that the apple MdCEP7 gene plays an important role in affecting the synthesis and transportation of auxin and further regulating plant root development.

The present invention discloses a SNP molecular marker associated with growth traits of Pelteobagrus vachelli, which is located at 41cM position of linkage group 14 of Pelteobagrus vachelli. The nucleotide sequence of the SNP molecular marker is shown as SEQ ID NO: 1, and a SNP site exists at the 26th position of the gene sequence: the base is G or C. The SNP molecular marker is located at 41cM position of linkage group 14 of Pelteobagrus vachelli. The invention also discloses a specific primer, a kit and a detection method for detecting the molecular marker. It is found that, The genotype ofthe SNP site at 26 bp of the gene at 41 cM in linkage group 14 of Pelteobagrus vachelli is GG, GC or CC is closely related to growth traits of Pelteobagrus vachelli, such as body weight, body width and total length. Therefore, the SNP molecular marker of the invention can be used for detecting or assisting in detecting growth traits of Pelteobagrus vachelli, and can be used for assisting selectionand breeding of Pelteobagrus vachelli. The invention has important guiding significance for breeding new strain of excellent growth characteristics of Pelteobagrus vachelli.

The invention discloses protein GPA4 related to plant glutelin transportation and storage and an encoding gene and application thereof. The protein is protein formed by an amino acid sequence shown in the SEQ ID NO.1 or protein which is formed after the amino acid sequence shown in the SEQ ID NO.1 is subjected to substitution and / or deletion and / or addition of one or more amino acid residues, related to plant glutelin transportation and storage and derived from the SEQ ID NO.1. Plants with the normal mature glutelin content can be cultivated by transferring the encoding gene of the protein into plants with the low mature glutelin content. The protein and the encoding gene thereof can be applied to genetic improvement of the plants.

The invention discloses a promoter Os023g37190 for regulating specific expression of exogenous gene in rice root. The promoter is a nucleotide sequence shown as SEQ ID NO:1. The promoter Os023g37190 disclosed by the invention can be used for constructing transgenic plant vectors specially expressed in root. The invention also provides a transgenic plant cell which contains the promoter sequence shown in the invention.

The invention belongs to the technical field of biology and particularly relates to a method for obtaining a large number of regenerated bambusa ventricosa mcclure by tissue culture, comprising the following steps: sterilizing young bambusa ventricosa mcclure branch buds as explants, forming calluses on an improved inducing culturing medium, inducing to generate embryogenic calluses, inoculating the embryogenic calluses on an improved differentiating culturing medium, inducing the embryogenic calluses to differentiate and germinate, inducing the embryogenic calluses to differentiate and root on a rooting culturing medium to form complete plants, and transplanting the plants into flower pots to finish the whole regeneration process of the bambusa ventricosa mcclure. The method can be used for obtaining the bambusa ventricosa mcclure at high frequency and is suitable for the genetic improvement of the bambusa ventricosa mcclure.

The invention belongs to the field of genetic engineering, and relates to plant starch synthesis-associated protein OsFLO10 as well as an encoding gene and an application thereof. The protein is shownin following (a) or (b): (a) protein consisting of an amino acid sequence shown in sequence 1 in a sequence table; (b) protein which is derived from the sequence 1, obtained by the amino acid sequence shown in the sequence 1 by substitution and / or deletion and / or addition of one or more amino acid residues and associated with plant gluten sorting. The plant starch synthesis-associated protein OsFLO10 affects synthesis of starch in plant endosperm. After the encoding gene of the protein is introduced into plants with abnormal starch synthesis, transgenic plants with normal starch synthesis canbe cultivated. The protein and the encoding gene thereof can be applied to plant genetic improvement.

The invention relates to a method for planting chunlan, which comprises that: 1, selecting seed, and marking number; 2, cultivating and recording the condition, picturing and storing; 3, artificial pollination, marking them as father and mother, and storing the cross record; 4, cultivating; 5, taking off the fruit and disinfecting; 6, without insects, cutting off the fruit, planting seed into cultivate medium, adjusting condition; 7, selecting green point seed, hurting and cultivating at medium into root stem; 8, transplanting into separating medium to grow into seed; 9, transplanting from the bottle to be cultivated via general method; 10, flowering.