9results about How to "Genetic improvement" patented technology

The invention discloses an Indel site simultaneously related to the pod number and the single plant yield of soybean, a molecular marker and application of the Indel site and the molecular marker, and belongs to the field of molecular marker-assisted breeding. The Indel site is located at 19bp, 855bp and 388bp of a soybean No.6 chromosome, and the polymorphism is an amplified fragment difference of 89bp. The detection method comprises the following steps: extracting a soybean genome DNA to be identified and carrying out PCR amplification by taking the soybean genome DNA as a template; detecting the size of a PCR amplification product fragment by 1% agarose gel electrophoresis; when the fragment of the amplification product is a small fragment, the soybean to be identified is judged to be a soybean line with multiple pods and high yield, and when the fragment of the amplification product is a large fragment, the soybean to be identified is judged to be a soybean line with small pods and low yield. According to the method, the detection and breeding cost is reduced, the cultivation effect of the soybean high-per-unit-yield variety is improved, the progress of soybean yield improvement is accelerated, and the soybean breeding time is shortened.

This invention relates to the fields of molecular markers and animal genetic breeding technology, specifically to a molecular marker located on chromosome 4 of pigs that is associated with body size and carcass traits, and its application. Using the F7 generation of a chimeric family as the research object, this invention employs whole-genome resequencing and GWAS analysis to study and identify the molecular markers located on chromosome 4 of pigs associated with body size and carcass traits. By optimizing the dominant alleles of these molecular markers, the frequency of dominant alleles can be increased generation by generation, enabling rapid and accurate selection of body size and / or carcass traits, accelerating the progress of pig genetic improvement, and thus effectively improving the economic benefits of pig breeding.

The present application discloses a plant pollen exine development related protein OsHIT3, a coding gene and application thereof. The plant pollen exine development related protein OsHIT3 of the present application influences the male gametophyte development process of a plant. Inhibition of the expression of the protein coding gene can lead to abnormal development of the male gametophyte of the plant and affect the formation of grains, so that a transgenic plant with abnormal pollen exine development and a transgenic plant with male sterility can be cultivated. The coding gene of the protein is introduced into a male sterile plant, so that a plant with normal development of male gametophyte can be cultivated. The protein and the coding gene thereof can be applied to genetic improvement of a plant.

The application discloses a rice endosperm development related gene OsTML, a coding protein and application thereof. The gene OsTML is a DNA molecule as described in the following 1), 2), 3) or 4): 1) a DNA molecule shown in SEQ ID NO. 1; 2) a DNA molecule shown in SEQ ID NO. 2; 3) a DNA molecule hybridized with the DNA sequence defined in 1) or 2) under stringent conditions and coding the protein; and 4) a DNA molecule having more than 90% homology with the DNA sequence defined in 1), 2) or 3) and coding a plant endosperm development related protein. The application further provides a protein coded by the gene, the protein affects the development of a plant endosperm, and the coding gene of the protein is introduced into a plant with abnormal endosperm development, so that a transgenic plant with normal endosperm development can be cultivated. The protein and the coding gene thereof can be applied to plant genetic improvement.

The present invention belongs to the technical field of molecular biology and genetic breeding, and discloses a molecular marker closely linked to the major effect QTL qON.C2 of the number of ovules per ovary of rapeseed and its application. The major effect QTL locus of the number of ovules per ovary located by using 201 strains of rapeseed DH population is qON.C2 The peak SNP marker closely linked to it is Bn‑scaff_15714_1‑p1900599, located at base 2,353,885 of chromosome C02 of the Darmor V4.1 reference genome of rapeseed, which can explain 15.4% of the phenotypic variance on average, with an average additive effect of ‑1.13. The PARMS marker designed using this SNP was used to test the remaining DH lines, and it was found that it was easy to operate and had clear typing, and had a good selection effect on the number of ovules per ovary of rapeseed.

The present invention belongs to the technical field of molecular biology and genetic breeding, and discloses a molecular marker BnMes-5A1 closely linked to the QTL of rapeseed methylselenocysteine ​​content and its application. The present invention obtains a SNP marker significantly associated with the methylselenocysteine ​​content of rapeseed, which is located at the 36898348th base position on the chromosome A05 of the Darmor-bzh v10 genome and can explain 5.1% of the phenotypic variation. Through auxiliary selection by the molecular marker, the screening efficiency of single strains of rapeseed containing higher methylselenocysteine ​​can be improved.

This invention discloses a PtoSAUR74 gene related to the adventitious root formation ability of poplar and its applications. The nucleotide sequence of the coding region of the PtoSAUR74 gene disclosed in this invention is shown in SEQ ID NO.1, and the protein sequence is shown in SEQ ID NO.2. Overexpression of the PtoSAUR74 gene in poplar significantly inhibits the length, number, and growth rate of adventitious roots, indicating that the PtoSAUR74 gene is a key regulatory gene affecting the development of adventitious roots in poplar, providing a key target for precise molecular regulation of poplar root growth. This invention also discloses a method for regulating the adventitious root formation ability of poplar. By regulating the expression of the PtoSAUR74 gene, adventitious root formation can be directly inhibited, providing new technical means and theoretical basis for the genetic improvement of poplar.

The invention discloses application of an OsMYB1 gene in regulation and control of nutritional indexes and taste quality of rice, and belongs to the technical field of biotechnology and plant genetic engineering. The invention provides an application of an OsMYB1 gene, an OsMYB1 protein coded by the OsMYB1 gene or a biological material with the OsMYB1 gene knocked out in regulation and control of nutritional indexes and taste quality of rice grains. The nutritional indexes comprise the amylose content; the taste quality comprises hardness, chewiness and / or adhesiveness; the nucleotide sequence of the OsMYB1 gene is as shown in SEQ ID No. 1; the amino acid sequence of the OsMYB1 protein is as shown in SEQ ID No. 2. Gene resources are provided for rice breeding, and the OsMYB1 gene is applied to rice breeding and has important application value and practical significance.

The invention relates to a molecular marker and application thereof, in particular to application of a ZmGAD gene and an InDel molecular marker in regulating and controlling the GABA content of a corn root system. The molecular marker ZmGAD-INDEL10842 provided by the invention has the characteristics of stable amplification, high detection efficiency and the like, provides an effective marker resource for molecular marker-assisted breeding of the GABA content of the corn root system, can promote genetic improvement of the stress resistance of the corn, and assists in cultivation of new high-yield varieties of the corn.