Method for obtaining a large number of regenerated bambusa ventricosa mcclure by tissue culture

A technology for tissue culture and plant regeneration, applied in the biological field, can solve problems such as difficulty in regeneration, and achieve high-frequency effects

Inactive Publication Date: 2012-07-18
NANJING FORESTRY UNIV
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Problems solved by technology

[0005] The present invention aims at the deficiencies and defects of the background technology, overcomes the problem of difficult regeneration in the tissue culture process of the small Buddha belly bamboo, designs a medium suitable for the regeneration of the small Buddha belly bamboo through the combination of different growth regulators, and completes the small Buddha belly bamboo plants The fastest regeneration time is only about 15 weeks

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  • Method for obtaining a large number of regenerated bambusa ventricosa mcclure by tissue culture
  • Method for obtaining a large number of regenerated bambusa ventricosa mcclure by tissue culture
  • Method for obtaining a large number of regenerated bambusa ventricosa mcclure by tissue culture

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Embodiment Construction

[0024] The operation steps of the method for obtaining a large amount of regenerated plants by the method of small Buddha belly bamboo tissue culture are as follows:

[0025] (1) selection of explants: choosing material in the experiment of the present invention is little Buddha belly bamboo ( Bambosa vantricosa ) regenerated shoots of sterile seedlings. ( figure 1 )

[0026] (2) Induction and maintenance of embryogenic callus: select regenerated shoots that have just germinated on sterile seedlings, and cut stems about 1.5 cm away from the shoot tip ( figure 2 ). After receiving the callus induction medium (MS+TDZ0.001mg / L+2,4-D6mg / L+NAA0.5mg / L), the callus formed after four weeks, and the formed callus was transferred to the callus proliferation medium (MS+5mg / L2,4-D+0.5mg / L6-BA+1mg / L naphthaleneacetic acid+100mg / LVc) for proliferation culture. ( image 3 )

[0027] (3) Differentiation of embryogenic callus: After 30 days of embryogenic callus culture, the callus wa...

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Abstract

The invention belongs to the technical field of biology and particularly relates to a method for obtaining a large number of regenerated bambusa ventricosa mcclure by tissue culture, comprising the following steps: sterilizing young bambusa ventricosa mcclure branch buds as explants, forming calluses on an improved inducing culturing medium, inducing to generate embryogenic calluses, inoculating the embryogenic calluses on an improved differentiating culturing medium, inducing the embryogenic calluses to differentiate and germinate, inducing the embryogenic calluses to differentiate and root on a rooting culturing medium to form complete plants, and transplanting the plants into flower pots to finish the whole regeneration process of the bambusa ventricosa mcclure. The method can be used for obtaining the bambusa ventricosa mcclure at high frequency and is suitable for the genetic improvement of the bambusa ventricosa mcclure.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for obtaining a large amount of regenerated seedlings of the small Buddha belly bamboo. Background technique [0002] Small Buddha belly bamboo belongs to the genus Bougainvillea, also known as gourd bamboo, Buddha bamboo, and dense knot bamboo. It is one of the important ornamental bamboos with special-shaped stalks as ornamental traits. There are generally two kinds of stalk shapes: the lower part of the normal stalk shape is slightly zigzag; the deformed stalk shape is shortened and swollen like a vase. The latter stalk shape is often used to make bonsai and garden cultivation for ornamental purposes. The bamboo is 25-60cm high, 0.5-2cm in diameter, and the internodes are relatively short, generally only 2-5cm, or shortened and swollen into a vase shape, which is a famous ornamental bamboo. Bamboo species have a broad market prospect (Chen Shuanglin, Yang Xi....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 丁雨龙钱维杰魏强林树燕
Owner NANJING FORESTRY UNIV
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