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Sequence related to red and yellow color properties of citrus peel and application of sequence

A technology of citrus peel and sequence, which is applied to the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., to achieve the effect of improving breeding efficiency and saving breeding costs

Active Publication Date: 2017-12-19
HUAZHONG AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports on the specific genetic locus, specific molecular genetic mechanism and screening of SNP molecular markers related to citrus peel traits.

Method used

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  • Sequence related to red and yellow color properties of citrus peel and application of sequence
  • Sequence related to red and yellow color properties of citrus peel and application of sequence
  • Sequence related to red and yellow color properties of citrus peel and application of sequence

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Embodiment Construction

[0024] The principles and features of the present invention are described below in conjunction with examples, which are only used to explain the present invention and are not intended to limit the scope of the present invention. The experimental methods in the following examples, unless otherwise specified, are conventional methods, such as molecular cloning experiment manuals such as sambrook (Sambrook J&Russell DW, Molecular cloning: laboratory manual, 2001), or suggested according to the instructions of the biochemical reagent manufacturer condition. The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0025] The DNA and peel samples of the hybrid population of tangerine and Citrus aurantium (planted in 2004) were obtained from the Citrus Breeding Engineering Center of Huazhong Agricultural University in Wuhan City, Hubei Province; the DNA and peel samples of more than 100 natural populations were ob...

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PUM

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Abstract

The invention relates to a sequence related to red and yellow color properties of citrus peel. The sequence is shown in SEQ ID NO:1, and is positioned at the upstream of a gene for encoding carotenoid splitting dioxygenase. The invention further provides application of the sequence in citrus breeding, and a method for predicting the color of citrus peel or the color of peel of a filial generation of citrus in the breeding process. The method comprises a step of detecting whether the sequence as shown in SEQ ID NO:1 at the upstream of the gene for encoding the carotenoid splitting dioxygenase exists or not, and / or detecting polymorphism of an SNP site in the sequence. The invention further provides a breeding method for changing the color of citrus peel. The sequence has great instruction significances for genetic improvement of appearance colors of citrus, breeding efficiency improvement and breeding cost reduction.

Description

technical field [0001] The invention relates to the field of citrus breeding, more particularly, to a sequence related to the red-yellow color trait of citrus peel and its application. Background technique [0002] Citrus is one of the most important economic crops in the world, the largest category of fruit in the world, and the third largest traded agricultural product in the world. Its peel color is one of the main characters of citrus peel appearance, and it is also an important symbol to measure the commerciality of a variety. As far as consumers prefer, red peel is the most popular appearance color of citrus. None of them are red, and some varieties with red peel have other disadvantages, and cannot be developed in large quantities. Therefore, the red peel trait is an important goal in the process of citrus breeding. Molecular assisted breeding using molecular markers co-segregated for target traits has the advantages of high efficiency, rapidity, and no environmental...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 邓秀新郑雄杰孙权谢宗周徐强柴利军
Owner HUAZHONG AGRICULTURAL UNIVERSITY
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