65results about How to "Reduce breeding costs" patented technology

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to growth traits of litopenaeus vannamei and application of the SNP molecular marker, and belongs to the technical field of biology. The SNP molecular markers 1-3 are remarkably related to the growth traits of the litopenaeus vannamei, genomic DNA of muscular tissue of the litopenaeus vannamei to be detected is extracted and then serves as template DNA to be subjected to PCR amplification and sequencing, the genotypes of the three molecular markers are determined, and the litopenaeus vannamei with obvious growth advantages can be rapidly screened out. The molecular marker can accelerate the breeding process of the improved variety of the litopenaeus vannamei, and provides a theoretical basis for mining application of related genetic markers in litopenaeus vannamei molecular marker-assisted breeding.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the testis size character of Hu sheep as well as a screening method and application of the SNP molecular marker, and belongs to the fields of genetic breeding and molecular biology. The marker is located in an intron region of a Hu sheep chromosome 3 LRRIQ1 gene and is remarkably related to the Hu sheep testis size character, and the accuracy and reliability of the SNP molecular marker are ensured through a scientific and rigorous screening method; according to the marker, Hu sheep individuals related to testis size characters can be rapidly and accurately screened out, the breeding efficiency and accuracy are improved, the breeding process is accelerated, the breeding cost is reduced, and the economic benefits are improved; the genetic diversity is protected, the accuracy of seminal fluid quality evaluation is improved, a powerful tool is provided for Hu sheep breeding work, and important basic data is also provided for researching a genetic mechanism of testis development and functions; the method can also be used as an auxiliary diagnosis tool to assist in identifying potential genetic defects or disease risks and promote progress and development of the breeding technology.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to growth traits of litopenaeus vannamei and application of the SNP molecular marker, and belongs to the field of molecular marker-assisted breeding of aquatic animals. The genome DNA of the muscular tissue of the litopenaeus vannamei to be detected is extracted as a template, the gene is subjected to PCR amplification and sequencing, the genotype of the molecular marker SNP1-3 is determined, and the molecular marker SNP1-3 is used as the SNP molecular marker for seed selection, so that the aim of quickly screening litopenaeus vannamei individuals with obvious growth advantages in the early stage is fulfilled. According to the molecular marker related to the growth traits of the litopenaeus vannamei, the breeding period can be greatly shortened, the fine breed breeding process of the litopenaeus vannamei is accelerated, the breeding efficiency is improved, and the breeding cost is reduced.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to lactation traits of buffalo and application of the SNP molecular marker, the SNP molecular marker is located at the 51st basic group of Chr1166691407, the SNP molecular marker is G or T, and the nucleotide sequence of the Chr1166691407 is shown as SEQ ID NO.1. The invention further discloses a preparation method of the SNP molecular marker related to the lactation traits of buffalo and application of the SNP molecular marker related to the lactation traits of buffalo. The SNP molecular marker which is most related to the peak milk yield of the buffalo is obtained by means of DNA extraction and sequencing, genome comparison, whole genome association analysis and the like, and the peak milk yield of the GG type buffalo is superior to that of buffalo with other genotypes by researching the remarkable association between the genotype of the SNP molecular marker and the peak milk yield of the buffalo; the method is applied to auxiliary breeding of buffalo strains or varieties with the peak milk yield characteristic, so that the breeding period is shortened, the breeding efficiency is improved, and the buffalo produced by the bred buffalo with the peak milk yield characteristic can be greatly improved.

The invention provides a larimichthys crocea fast-growing character associated SNP marker combination and breeding application, the combination is prepared from a probe for detecting 10 SNP molecular marker combinations of larimichthys crocea fast-growing characters, and information of positions of all SNP molecular markers on chromosomes, alleles with changed sites and reference genes is shown in a table 1. According to the SNP marker combination disclosed by the invention, the optimal large yellow croaker individuals with fast-growing potential and a certain scale can be selected from the breeding offspring in advance for breeding and breed conservation, and the large yellow croaker individuals are bred to the parent without breeding and breed conservation of a large number of offspring seeds in the breeding process, so that the breeding cost can be greatly reduced. The SNP marker combination provided by the invention can solve the problem that non-lethal detection of the growth speed of the large yellow croaker cannot be realized in a traditional breeding mode, and can ensure smooth development of fast-growing character breeding work of the large yellow croaker.

The invention discloses a novel SNP (Single Nucleotide Polymorphism) molecular marker related to a pig daily gain character and an application of the SNP molecular marker in breeding. The SNP molecular marker is Agt located at the 15401211bp position of the No.17 chromosome of a pig genome Ensembl Sscrofa11.1 version; g mutation. Through the application of the SNP molecular marker, the daily weight gain performance of large Duroc commercial pigs and Duroc boars thereof can be improved, so that the growth speed is increased, the on-the-market time is shortened, and the economic benefits are improved; and through the SNP molecular marker and the application thereof, the breeding period can be greatly shortened, the breeding cost is saved, and the market competitiveness is improved.

The invention discloses an InDel marker primer for identifying authenticity of sisal hemp interspecific hybridization F1 generation and application of the InDel marker primer. According to re-sequencing data of parent varieties of sisal hemp, an InDel marker primer for identifying the authenticity of a hybrid F1 generation of sisal hemp '292' and 'Induced 7' is developed, identification is carried out in a seedling stage of the hybrid F1 generation, and a result shows that offspring which can simultaneously amplify male parent and female parent characteristic bands is a true hybrid; the hybrid seeds are regarded as false hybrids when any one stripe is lacked or two stripes are lacked, so that the detection purity of the F1-generation real hybrid seeds is improved, and the identification time is advanced. Meanwhile, compared with a traditional hybrid identification method, the method for identifying the authenticity of the sisal hemp variety interspecific hybridization F1 hybrid is rapid, convenient, accurate, reliable, short in breeding period and low in breeding cost, breeding target offspring can be screened in advance, the identification efficiency of sisal hemp interspecific hybrids is effectively improved, and the method is suitable for popularization and application. And a more effective method is provided for rapidly identifying the authenticity of sisal hemp interspecific filial generations.

The invention discloses a molecular marker influencing the early weight of sheep as well as a primer and application of the molecular marker. According to the molecular marker capable of influencing the early weight of the sheep, two complete linkage sites of NC056056.1: g.26656G > A and NC056056.1: g.26473C > T of NR4A1 genes of the sheep, and three genotypes of GGCC, AGCT and AATT are shared. The weaning weight of the AGCT type sheep at the age of 45 days and the average daily gain from newborn to weaning are obviously higher than those of GGCC type sheep. The invention relates to a method for screening an early-stage rapid growth sheep variety (line), which comprises the following steps: detecting the G / A polymorphism of a NR4A1NC056056.1: g.26656 site and the C / T polymorphism of a NC056056.1: g.26473 site of sheep, and selecting AGCT type individuals for breeding. The polymorphic site can be used for early auxiliary selection of sheep growth traits, the selection accuracy is improved, and the breeding process is accelerated.

The invention discloses a group of molecular markers related to the oil yield of carya illinoensis and application of the molecular markers, and relates to the technical field of biology. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO. 1-2. The molecular marker disclosed by the invention can be used for marker assisted selection (MAS) and genome selection (GS) of the carya illinoensis, so that the genetic improvement progress of the variety of the carya illinoensis can be accelerated.

The invention relates to the technical field of molecular biology, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker of a Brassica napus Bolima cytoplasm sterile gene, a primer group, application and a method. The SNP (Single Nucleotide Polymorphism) molecular marker is BN9000344. The primer group comprises a primer group BN9000344K01X, a primer group BN9000344K01Y and a primer group BN9000344K01C, and the primer group comprises a primer group BN9000344K01X, a primer The application refers to the application of the SNP molecular marker in detecting whether the brassica napus is of the Brassica napus sterile cytoplasm type or not. The method is used for determining whether the brassica napus is of the Brassica napus sterile cytoplasm type or not. The method can rapidly and accurately detect whether the brassica napus contains the Brassica napus Bolima cytoplasmic male sterility gene or not, and is suitable for large-scale and high-throughput Brassica napus Bolima cytoplasmic male sterility gene identification (such as purity identification of sterile line and maintainer line parents) and screening.

The invention discloses a 5-HT1B gene molecular marker related to growth of penaeus vannamei boone and application, and belongs to the technical field of molecular marker-assisted breeding of aquatic animals. The sequence of the molecular marker is shown as SEQ ID NO.3, the molecular marker comprises five sites of E3-157G / A, E3-454C / G, E3-469C / T, E3-478G / C and E3-514G / T, and the dominant genotypes are AA or AG, GG or CG, CT, CG and TT or GT in sequence. The invention further provides a haplotype molecular marker composed of the E3-157G / A and the E3-514G / T, and the haplotype molecular marker comprises the E3-157G / A and the E3-514G The penaeus vannamei feed can be used for culture and improvement of penaeus vannamei; the method is not limited by the development stage, and can provide technical means and theoretical support for early breeding of penaeus vannamei individuals / families / varieties; the method can further shorten the breeding time and reduce the breeding cost.

The invention provides a method for creating a pop corn inbred line, and belongs to the technical field of new variety cultivation. The invention provides a method for creating a pop corn inbred line, which comprises the following steps: selecting pop corn germplasm to hybridize with other types of corns to obtain F1-generation seeds; burst corn germplasm is used as a female parent for backcrossing, then BC1F2 or BC2F2 seeds are obtained through planting and selfing, and bursting type grain seeds are selected; hybridizing the burst type grain seeds with a maize haploid induction line containing Ga1-s; hybridized clusters are taken after pollination, embryos are separated and cultured, and haploid embryos with haploid characteristics are selected; inducing the haploid embryo into a seedling, then inducing chromosome doubling, and selfing to obtain the pop corn selfing line. By utilizing the method, the pop corn inbred line can be efficiently, accurately and quickly created, the generation probability of haploid false positive is greatly reduced, the identification efficiency is remarkably improved, and the breeding cost is reduced.

The invention discloses a molecular marker Site1773 closely linked with a melon leaf color yellowing character and application of the molecular marker Site1773, and belongs to the field of plant molecular genetic breeding research. The molecular marker is used for detecting leaf yellowing characters in a plurality of natural muskmelon populations, DNA (deoxyribonucleic acid) of a material to be selected is used as a template, a primer pair of the molecular marker Site1773 is used for PCR (polymerase chain reaction) amplification, an amplification product is subjected to polyacrylamide gel electrophoresis, field character analysis is combined, and the identification accuracy rate of the molecular marker in the seedling stage is 100%. The molecular marker has very important value in muskmelon production practice and breeding. The operation method is simple, convenient, high in stability, rapid and accurate, and a novel auxiliary selection method is provided for molecular breeding of muskmelons.

The invention relates to the field of wheat breeding, in particular to a rapid breeding method of waxy wheat. The invention provides a rapid breeding method of waxy wheat, which combines a plurality of experimental techniques such as tissue culture, KASP molecular marker and iodine staining identification, and can rapidly create a new line of waxy wheat. According to the method, the breeding time can be effectively and remarkably shortened, and a new method is provided for wheat variety improvement.

The invention provides a larimichthys crocea anti-flow character associated SNP marker combination and breeding application, the combination is prepared from a probe of 13 SNP molecular marker combinations for detecting larimichthys crocea muscle elasticity characters, and information of positions, alleles with changed sites and reference genes of all SNP molecular markers on chromosomes is shown in a table 1. According to the SNP marker combination disclosed by the invention, the optimal larimichthys crocea individuals with the anti-flow potential and a certain scale can be selected from the breeding offspring in advance for breeding and breed conservation, and the larimichthys crocea individuals are bred to the parent without breeding and breed conservation of a large number of offspring seeds in the breeding process, so that the breeding cost can be greatly reduced. By utilizing the SNP marker combination, the problem that non-lethal detection of the muscle elasticity of the large yellow croaker cannot be realized in a traditional breeding mode can be solved, and smooth development of anti-flow character breeding work of the large yellow croaker can be ensured.

The invention relates to the technical field of molecular breeding, and discloses a molecular marker related to the LDL-C level of porcine serum, a detection reagent and a breeding method. The invention also discloses a primer group for identifying the molecular marker, and the primer group is used for amplifying the following SNP sites: SNP01, which are obtained on the basis of a pig reference genome Sscrofa11.1 and are positioned on 112bp, 008bp and 261bp of a third chromosome, and the genotype is A / G; sNP02 is located at 112bp, 008bp and 354 bp of a third chromosome, and the genotype of SNP02 is A / G; sNP03 is located at 112bp, 008bp and 583bp of the third chromosome, and the genotype of SNP03 is A / G. The primer group for identifying the molecular marker can quickly and effectively identify the LDL-C level of pig serum, and is used for assisting in breeding pig strains with low cholesterol or specific lipid metabolism characteristics.

This invention provides an Indel molecular marker closely related to the soluble sugar content of pepper fruit and its application, belonging to the technical field of nucleic acid-based assay methods. The nucleotide sequence of the Indel molecular marker is shown in SEQ ID NO.1 of the sequence listing; the Indel molecular marker is located at positions 5274716-5274727 of the full-length sequence of chromosome 3 of the pepper reference genome. Primers for amplifying the Indel molecular marker include a forward primer and a reverse primer. The nucleotide sequence of the forward primer is shown in SEQ ID NO.2 of the sequence listing, and the nucleotide sequence of the reverse primer is shown in SEQ ID NO.3 of the sequence listing. This molecular marker can be directly applied in early generations of breeding, avoiding the destructive sampling of chemical detection, significantly reducing breeding costs, effectively shortening the breeding cycle, and greatly improving the selection efficiency of molecular marker-assisted breeding in peppers.

The utility model discloses a kind of water culture devices for yunnan rhizoma polygonati breeding, it is related to yunnan rhizoma polygonati cultivation technical field, including culture rack, the culture rack is slidably connected with multiple water storage channels, and the water storage channel upper end is provided with culture plate;Multiple planting holes are evenly provided in the culture plate, and the culture plate upper end is connected with multiple fixed components, and fixed component is located planting hole outer end.The utility model design structure is reasonable, it can simultaneously carry different batches, different genotypes yunnan rhizoma polygonati breeding material, while independent water supply head, gate plate can individually adjust the water supply of each water storage channel, water supply frequency, realize the accurate management of "one tank one strategy", satisfy the individualized demand of different breeding material, to improve the success rate of breeding, can avoid the problem of "excessive supply" or "insufficient supply" caused by unified water supply, accurately match the demand of each growth stage of yunnan rhizoma polygonati, save water resource, nutrient solution and other consumables, reduce breeding cost.

The present invention belongs to the technical field of molecular genetics, and specifically relates to a SNP site related to the fine wool rate of fine wool sheep and its application. The SNP molecular marker described in the present invention is located at the A / T mutation of the 236304249th nucleotide site on chromosome 1 of the international sheep reference genome Oar_v4.0 version. The present invention also provides the use of a reagent for detecting the above-mentioned SNP site in detecting the fine wool rate trait of fine wool sheep or in molecular marker-assisted breeding of fine wool sheep, which has great application value in fine wool sheep variety screening, variety identification or molecular marker-assisted breeding; it is used for early breeding selection, shortening the generation interval, accelerating the breeding process, saving a lot of breeding costs, and has broad market prospects.

The present invention provides a molecular marker related to the water-power trait of pork and its application. The molecular marker is a g.152858710C>T polymorphic site on chromosome 6 of the pig reference genome Sscrofa11.1, specifically the 160th site of the nucleotide sequence shown in SEQ ID NO.1, and the Y of the site is C or T, with C / T polymorphism. The present invention also provides a method for screening pigs with excellent water-power, which can screen pigs at an early stage, effectively solving the problem that muscle drip loss in actual production cannot be screened by live measurement, reducing breeding costs, and the method has high accuracy, low detection costs, and can realize automated detection, and has high practical application value in pig breeding.

The present application relates to the technical field of plant breeding, and particularly relates to a molecular marker and application thereof in breeding soybean varieties with high oil content and high protein content. The molecular marker is constructed based on a SNP site; based on the genome version number Wm82.a2.v1, the SNP site is located at the 8th chromosome of soybean at the 9020004th position, and the polymorphism is T / A. The application comprises: (1) predicting or detecting the oil content or protein content of soybean; (2) identifying or breeding soybean varieties with high oil content or protein content; (3) molecular marker assisted breeding of soybean; (4) improvement of soybean varieties related to oil content or protein content; (5) improvement of soybean germplasm resources. The molecular marker provided by the present application has the characteristics of double trait synergistic correlation, strong specificity, efficient detection process and the like, and can be widely applied to the scenes of molecular marker assisted breeding of soybean, rapid evaluation of germplasm resources and the like, and provides key technical support for breeding of soybean varieties with double optimal traits.

The invention relates to the technical field of molecular breeding of crops, in particular to a KASP molecular marker related to the size of soybean seeds and application of the KASP molecular marker. The molecular marker group comprises three SNP (Single Nucleotide Polymorphism) sites located in an intron region of a gene GmW82.04G097200 of a soybean T2T genome, wherein an SNP1 site is located at 8152017bp, and T / C nucleotide polymorphism exists; the SNP2 site is located at 8152443bp, and the A / G nucleotide polymorphism exists; the SNP3 site is located at 8153901bp, and C / G nucleotide polymorphism exists in the SNP3 site; alleles of the three SNP sites are combined to form three haplotypes corresponding to soybean seeds in size. The method has the advantages that three groups of specific KASP primer groups are provided, and genotypes of different soybeans are rapidly distinguished by virtue of PCR amplification and fluorescence signal detection, so that accurate screening of large-grain phenotype individuals is realized, the breeding period is effectively shortened, and the breeding input cost is reduced.

The invention relates to a CD99 gene molecular marker primer related to duck egg yellow weight and application of the CD99 gene molecular marker primer, and belongs to the technical field of biology. The molecular marker is located at the 135221179th basic group of a No.1 chromosome of a duck reference genome GCF015476345.1 ZJU1.0 version, has A / T polymorphism, has three genotypes of A / A, A / T and T / T, and is located in an intron region of a CD99 gene. The egg yolk weight of the A / A genotype individual of the CD99 gene is higher than that of the A / T genotype individual and that of the T / T genotype individual; the molecular marker is used for screening the yellow weight index of the high-quality duck eggs and improving the rate of the high-quality duck eggs, can be applied to early selection of the high-quality ducks, is beneficial to accelerating the breeding progress, improving the breeding accuracy and reducing the breeding cost and the production cost, and has great economic application value and breeding value for the high-quality ducks.

The invention discloses a molecular marker for high-throughput identification of a wheat scab site Qfhb7EL and application of the molecular marker. The nucleotide sequences of primers of the molecular marker are as shown in SEQ ID No. 1 to SEQ ID No. 4. Specific primers are designed according to the homologous sequence of the Fhb7 gene in the triticum elongatum, the specific primers and the Actin gene of wheat form a primer pair, population typing is achieved under recognition of different fluorescence, and the reliability of the marker is verified through molecular experiments. Results show that the identification of the wheat scab-resistant site Qfhb7EL in the wheat background can be quickly and accurately realized in a high-throughput manner, and the method has a very high application value for auxiliary selection of new wheat scab-resistant varieties.

The present application relates to a kind of KASP molecular markers related to common pakchoi leaf purple and its application, by QTL-seq technology and genetic map location, the main effect QTL site related to leaf purple located on the 3rd chromosome of common pakchoi is found, and further developed the molecular marker closely linked to site, by the seed or seedling leaf extraction DNA of color unknown common pakchoi material, combined with the KASP primer of the present application carries out KASP molecular marker genotyping test, the color of common pakchoi can be predicted by the genotyping result of marker, without using artificial identification method, can obviously improve breeding efficiency, save breeding cost.

The application discloses application of a molecular marker related to a pig fattening period average daily feeding time trait, relates to the technical field of biology, and is characterized in that the application is any one of A1) to A4) below: A1) detecting or assisting in detecting a pig fattening period average daily feeding time; A2) identifying, assisting in identifying a pig fattening period average daily feeding time; A3) preparing a product for detecting or assisting in detecting a pig fattening period average daily feeding time; and A4) preparing a product for identifying, assisting in identifying a pig fattening period average daily feeding time. The application of the molecular marker related to the pig fattening period average daily feeding time trait discovers that a site of A / C at 270462812 of a chromosome 1 in a pig reference genome Sus_scrofa.Sscrofa11.1 has a significant influence on the pig fattening period average daily feeding time.

The present invention discloses an Indel molecular marker tightly linked to a major effect QTL locus of corn resistance to southern rust and its application. The Indel molecular marker is present in the confidence interval of the QTL locus of a corn plant showing resistance to southern rust, and is missing in the confidence interval of the QTL locus of a corn plant showing susceptibility to southern rust. The present invention provides an effective and reliable molecular marker for the identification or screening of corn resistance to southern rust, and is applied to the genetic improvement of corn resistance to southern rust and its breeding, which can effectively shorten the breeding period, speed up the breeding process, improve the breeding efficiency, speed up the process of screening excellent germplasm resources, and provide a new direction for reducing breeding costs. For example, it can be used for corn seed production by pollinating and preserving seeds for corn plants containing Indel molecular markers. The present invention provides a new direction for the screening, identification and breeding of corn resistance to southern rust by molecular assisted selection.

The invention discloses a method for selectively breeding an excellent DH line of a crop by using a whole genome, and belongs to the technical field of biological breeding. The whole genome selection method comprises the following steps: (1) establishing a statistical genetic model; (2) calculating a marker effect value # imgabs0 # and a population mean value # imgabs1 #; (3) calculating a breeding value based on the marker effect value # imgabs2 # and the population mean value # imgabs3 #; (4) calculating an average breeding value; and (5) selecting an excellent DH line based on the average breeding value. By adopting the whole genome selection method disclosed by the invention, not only can the excellent DH line be efficiently and accurately bred, but also the breeding cost can be greatly reduced and the breeding efficiency can be greatly improved.

This invention relates to the field of molecular marker technology, specifically to molecular markers for identifying early-forming medicinal agarwood. Using these molecular markers, it is possible to identify agarwood with early-forming characteristics, which is of great significance for the identification of agarwood germplasm resources and molecular marker breeding.

The invention discloses a tetranychus cinnabarinus resistant cassava variety breeding method, and belongs to the field of crop breeding, and the method comprises the following steps: S1, seedling transplanting: carrying out warm water seed soaking treatment on harvested hybrid seeds, placing the hybrid seeds in a nutrition bag, obtaining seedlings, and transplanting the seedlings into a cultivation pot after the seedlings grow for a period of time. According to the breeding method of the cassava variety resistant to tetranychus cinnabarinus, through mite resistance identification and excellent strain screening, single plants which may become excellent varieties in the future are selected in advance, single plants without target characters and poor characters are eliminated, the cassava variety resistant to tetranychus cinnabarinus is bred, the breeding period is shortened, the breeding cost is greatly saved, and the breeding method is suitable for large-scale popularization and application of the cassava variety resistant to tetranychus cinnabarinus. According to the method, the breeding efficiency is improved, the yield of cassava is increased, sustainable development of the cassava industry is promoted, meanwhile, the breeding process is simple, the breeding period is short, the breeding cost is low, the breeding efficiency is greatly improved, and sustainable development of the cassava industry is promoted.