549results about How to "Reduce breeding costs" patented technology

The invention discloses an intelligent rapid seedling propagation and breeding device which contains crystal energy. The intelligent rapid seedling propagation and breeding device which contains the crystal energy is composed of following modules of a base seat, a cultivation bed, a light hood, a growth information generator, an LED (light emitting diode) plant growth light, a semiconductor refrigeration and heating device, a heat preservation barrel, a nutrient solution barrel, a cool air pipe, a mist spray pipe, a rotary type mist spray head, a water level adjusting mouth and a seedling breeding execution system. The intelligent rapid seedling propagation and breeding device which contains the crystal energy is mainly characterized in that a twelve face crystal in a trigonal crystal system is arranged above the intelligent rapid seedling propagation and breeding device which contains the crystal energy, plant rooting and sprouting information is generated, growth information is conducted into a plant through the polyethylene light hood in a pyramid shape so as to start a rooting and sprouting growth mechanism of the plant, positions of the sun are figured out through sunlight according to sun traces of the LED plant growth light and the seedling breeding execution system, and manual sunlight suitable for plant varieties is intelligently controlled through a Zigbee protocol. The intelligent rapid seedling propagation and breeding device which contains the crystal energy is short in breeding cycle, achieves a developed root system for the plant, and can be widely used in industrialization seedling development and usage.

The invention discloses a method for determining optimal SNP quantity as well as performing genome selective breeding on production performance of large yellow croakers through selection markers. The method comprises the following steps: performing phenotype determination and genomic sequencing on the production performance of individuals of a reference group to obtain SNP locus; screening out the qualified SNP locus and supplementing the deleted genotype; dividing the reference group into a training set and a validation set to perform hybridization validation; screening the SNP locus most remarkably associated with the character through single marker analysis, and then calculating GEBV of individuals of the validation set only by using the locus and through a GBLUP method; further obtaining breeding value estimation accuracy under each screening SNP quantity; finally determining the optimal quantity of SNP screening; and according to the optimal quantity, calculating the GEBV by the GBLUP method, further obtaining the breeding value estimation accuracy and performing genome selective breeding according to the value size. By the method, the genomic selection cost on the production performance of the large yellow croakers can be reduced remarkably.

The invention relates to detection primers for molecular markers in close linkage with a major QTL (Quantitative Trait Locus) of wheat ear length and an application of the detection primers. The detection primers for the molecular markers include an upstream primer, having a nucleotide sequence as shown in SEQ ID NO.1, of the molecular marker XWMC112 and a downstream primer, having a nucleotide sequence as shown in SEQ ID NO.2, of the molecular marker XWMC112, and an upstream primer, having a nucleotide sequence as shown in SEQ ID NO.3, of the molecular marker XCFD53 and a downstream primer, having a nucleotide sequence as shown in SEQ ID NO.4, of the molecular marker XCFD53. The molecular marker XWMC112 and the molecular marker XCFD53 are studied and found for the first time, and can be used for quickly determining whether a wheat variety or strain has the QTL of the ear length, and thus quickly screening out the wheat variety or strain having the QTL for increasing the ear length, and therefore, the breeding progress of a high-yield wheat variety can be accelerated.

The invention relates to a factory propagation method for detoxified red sweet potato. The method comprises the step of detoxifying test-tube plantlets, breeder seeds, stock seeds and improved variety. The method specifically comprises the following steps: firstly, cleaning potato blocks, using 800-times diluted solution of 50% carbendazim for disinfecting for 20min and then performing 26 DEG C constant temperature sprouting; adopting a high/low temperature alterative treating mode of treating for 16 hours at 26 DEG C and treating for 8 hours at 35-40 DEG C in each day for continuously treating for about 30 days when the buds grow to 0.1-0.5cm; performing stem apex detoxification, rapid tissue culture propagation, rooting and seedling hardening, rapid water culture propagation, potato vine transplanting and breeder seeds propagation; and using the breeder seeds for rowing potato and seedling in the greenhouse with a fly net in the next year; transplanting into field, performing field management, controlling pests, timely harvesting and storing the stock seeds before frosting and propagating the excellent stock seeds. According to the invention, the detoxified seedlings can be subjected to factory propagation within a short period of time, the production cost of the detoxified seedlings can be reduced and the lastly acquired detoxified improved variety is convenient for the peasant to plant, transport and store.

The invention discloses a tissue-culture rapid propagation method for pyrus betulaefolia bunge. The tissue-culture rapid propagation method for the pyrus betulaefolia bunge comprises disinfecting on explants, inducing buds, propagating test tube seedlings, inducing calluses, inducing rooting and transplanting exercising seedlings. During the process of inducing rooting, the calluses are firstly induced and then the rooting is induced; obtained rooting test tube seedlings are performed seedling exercising in a triangular flask, then transferred to perlite to domesticate the exercising seedling and finally transplanted. Compared with the traditional method, the tissue-culture rapid propagation method for the pyrus betulaefolia bunge has the advantages of being high in rooting rate and survival rate of transplanting, high in propagation rate, low in cost, convenient to popularize, good in genetic stability and suitable for the pyrus betulaefolia bunge.

The invention provides an ancient tea rapid cultivation method. The method comprises the steps of 1, site selection of nursery land, wherein dry land, paddy soil land, sandy loam soil land or yellow soil land which is flat, capable of achieving drainage and irrigation, fertile, deep, good in structure and high in air permeability is selected; 2, establishment of a seedbed; 3, building of a sun shelter; 4, cuttage, wherein cutting wood is 3-4 cm long with a leaf reserved, a 100 mg/L solution is prepared with rooting powder, and the root of the cutting wood is soaked for 2 hours; 5, application of a film; 6, management of the nursery land; 7, outplanting. According to the method, female parent cultivation is not needed during cutting wood picking, and practice proves that cultivation cost is low, the basic characters of cultivated plants can be guaranteed, survival rate is high, operation is easy, cultivation time is short and is shortened by one third compared with a traditional cuttage method, and transplanting survival rate is increased especially so that requirements for storage, utilization and research of ancient tea germplasm resources can be met.

The invention relates to a new Ulmus pumila cv.jinye seedling raising technique, in particular to an Ulmus pumila cv.jinye root-reserved and branch-buried seedling raising method which has new breakthrough in breeding speed of the Ulmus pumila cv.jinye. The method is characterized in that 1. selecting all lateral branches at one side with large lateral branch amount of a plant while treating a maternal plant, and removing all lateral branches at another side; 2. performing primary branch burying, selecting branches with the length more than 30cm for burying, and burying branches with the length less than 30cm into soil with principal branches; and cutting 2-3 cuts at the lowest part of a bent part of the branches buried in the soil; and 3. selecting the branches with the length more than 30cm for secondary branch burying by new lateral branches growing in the primary branch burying and new branches growing on unburied branches in June; performing tertiary branch burying on the branches with the length more than 30cm, wherein, the branches grow into new plants in autumn; and the treatment method in the secondary branch burying and the tertiary branch burying are the same. The method has the advantages of high propagation coefficient, rapid propagation speed, less expense and low cost.

The invention discloses a method for rapidly obtaining a large number of wheat double-haploid homozygous populations. According to the method, based on a wheat and corn double-haploid breeding technology, through the key links of wheat and corn sowing at different stages, haploid induction, transplantation and vernalization, reduplication and transplantation and winter sowing treatment, after wheat young embryos differentiate into haploid seedlings, transplantation, vernalization and haploid plant reduplication are completed in the same year without over-summer treatment, wheat double-haploid seeds can be harvested in the same year, the requirement for sowing once more can be met after the harvest on the perspective of time, a large number of stable new strains of the wheat homozygous populations can be obtained within one year, the requirement for identifying the new strains at the next stage is met, the natural environment is utilized to the maximum degree, and the time of wheat double-haploid breeding is shortened to one year from two to three years. In this way, the wheat breeding speed is increased, the breeding efficiency is improved, the breeding cycle is shortened, and the breeding cost is lowered.

The invention belongs to the field of molecular marker screening, and relates to a molecular marker for controlling the flowering phase of cabbage type rape and applications of the molecular marker. The molecular marker is cloned from a BnaA 10.CO gene, is located on the A10 chromosome of the cabbage type rape, and is a key gene of a photoperiod route in a flowering phase regulation network. BnaA10. CO gene segments are cloned from winter rape and spring rape, and the sequences of the segments are shown as SEQ ID NO: 1 and SEQ ID NO: 3. Primers of the molecular marker related to the floweringphase are shown as SEQ ID NO: 5 and SEQ ID NO : 6. The transformation proves that the gene is derived from an allele of winter rape, and the effect of promoting flowering of the gene is stronger thanthat of the allele of the spring rape. The allele derived from the winter rape can shorten the growth period of half-winter rape. The molecular marker developed according to the InDel differences ofthe 25th-27th sites of the BnA 10.CO gene region can be used for early-maturing breeding of rape.

The invention relates to the technical field of animal molecular markers, in particular to a molecular marker related with a diameter character of sheep wool fibers as well as a specific primer and application thereof in selection of diameter character of Chinese Merino sheep wool fibers. The molecular marker related with the diameter character of the sheep wool fibers is located at the No.30 exonof the No.21 chromosome of FAT3 gene. The molecular marker has the advantages that the function of using rs425144268 SNP site as the molecular marker of the diameter of the sheep wool fibers is disclosed for the first time, and the function of applying the rs425144268 SNP site to the selection of the diameter character of the Chinese Merino sheep wool fibers is disclosed for the first time; whenthe excellent diameter character of the wool fibers is identified by the molecular marker related with the diameter character of the sheep wool fibers, the operation is simple, and the sheep with smaller fiber diameter can be screened in an assisting way; because the detection level of the molecular marker is started, the accuracy of variety selection is improved, and the detection efficiency is improved.

The invention relates to molecular markers of paddy endosperm low amylose content gene Wx-mq, which belong to the field of molecular genetics. Two pairs of PRC molecular marker primers for specifically amplifying paddy endosperm low amylose content control gene Wx-mq and an allelic gene Wx-b thereof are used, wherein a forward general primer 1Wx-b-F of the Wx-b and the Wx-mq, a reverse primer 11Wx-b-R of the Wx-b and a reverse primer 11Wx-mq-R of the Wx-mq perform common amplification on the DNA of japonica rice varieties or materials, and can distinguish genotypes of the Wx-mq gene and the allelic gene thereof accurately. Developed peculiar PCR markers of the allelic gene are utilized to perform the paddy endosperm low amylose seed breeding and the improvement of rice quality.

The invention discloses a method for breeding a rehmannia toxin-free seedling. The method disclosed by the invention comprises the following steps: production of an original rehmannia seedling: cutting a rehmannia toxin-free test-tube plantlet into stem segments provided with axillary buds with the length of about 1cm on a clean bench, inoculating the stem segments onto a culture medium with the concentration of MS+NAA of being 0.01-0.05mg/L and the concentration of 6-BA of being 0.05-0.5mg/L, and culturing under the culture conditions of light intensity of 2000lux, pH of 6.0 and temperature at 25-30 DEG C to obtain a toxin-free test tube plantle, wherein, reproduction coefficient can reach about 5 after 30-40 days; cutting the rehmannia toxin-free test tube plantlet into the stem segments provided with the axillary buds with the length of about 1cm on the clean bench, inoculating the stem segments onto a culture medium with the concentration of 1/2MS+PPP<333> of being 0.02-0.2mg/L, and culturing in a culturing room to obtain a rooted toxin-free test tube plantlet, wherein, roots are formed within 7 days and a large number of roots are formed within 15-20 days; and transplanting the test tube plantlet into a greenhouse to carry out acclimation so as to finally obtain the toxin-free seedling, wherein, transplanting survival rate can reach up to more than 95%. The method disclosed by the invention has the advantages that cost for breeding the toxin-free seedling is low and yield is high.

The invention relates to a method for plant leaf bud shoot cutting propagation all year around and relates to the technical field of vegetative propagation. The method aims to overcome the defects that in the prior art, nursery stock seedling propagation is large in variation and part of nursery stock is low in maturing rate, and tissue culture propagation is high in cost and difficult in technology management, and minimum stems of existing fine seed nursery stock leaf buds are adopted as female parent cuttage materials for cuttage. The method includes the steps of solar greenhouse or movable greenhouse facility and planting bed preparation, leaf bud selection, cuttage time and method determination and seedling period management. The propagation coefficient of seedlings cultivated in the method can be progressively increased in a multiplied mode, the survival rate of the seedlings can reach 90%, roots are developed and grow vigorously, and the transplantation survival rate can exceed 95%. The method has the greatest advantages that mass production and industrialized production can be achieved, propagation does not need professionals, and the method is easy to operate, low in individual cost, high in propagated seedling survival rate and easy to popularize.

The invention discloses a high-efficiency polymerization method for apple cross breeding. The cross breeding is the most important and most effective way of the breeding of the apples, but the evaluation and the screening of crossbreed seedlings and cross breeding fruits take a lot of work and labors, steps of the cross breeding method are complicated, and the improper technology of each step can cause the failure of the breeding. The breeding period of the apples is long and needs at least 20 years from the cross breeding to the new species selecting and breeding. The method optimizes the key steps of the cross breeding of the apples and overcomes the defects of the conventional breeding method. The method adopts the technical schemes of anther collection; artificial cross breeding; cross breeding management; cross breeding seed collection and pregermination; cross breeding young seedling management; early selection of cross breeding seedlings; primary selection and reselection; and excellent series area demonstration. Compared with the conventional method, the high-efficiency polymerization method specifically and effectively selects indexes, can shorten the breeding period of the apples by 3-4 years and save the breeding cost of the apples by 15-20 percent, and the method system achieves the international advanced level.

The invention provides a molecular marking method for sheep wool curl, which relates to a molecular marking method, which comprises the following steps of: 1, extracting the sheep genome deoxyribonucleic acid (DNA) for carrying out polymerase chain reaction (PCR) amplification and then carrying out enzyme digestion to obtain enzyme digestion products; 2, carrying out electrophoresis separation onthe enzyme digestion products and carrying out genotype judgment according to the electrophoresis separation results; 3, carrying out association analysis and evaluating least square mean value of the property to obtain a result that the wool curl of individuals with the TT genotype type and TC genotype in the three genotypes is obviously higher than the wool curl of individuals with the CC genotype; and 4, dividing experiment groups into three types according to the result in the third step to complete the operation of the molecular marking method. The molecular marking method can be used for wool curl and fineness molecular marking auxiliary culture, the early period selection of the breeding sheep can be realized, and the sheep can be selected after the birth, so the breeding cost can be reduced, the breeding process of high-quality fine wool sheep is accelerated, the operation is simple, the accuracy is high, the efficiency is high, and the automatic detection can be realized.

The method relates to a method for creating winter dwarf male-sterile wheat with blue seeds. The method is characterized in that winter (or semi-winter) wheat is used as a male parent under a natural ecological condition in a winter (or semi-winter) ecological area; the dwarf male-sterile wheat with blue seeds is continuously subjected to back crossing with the male parent; the seeds of dwarf male-sterile wheat with blue seeds are selected from progeny individuals of the dwarf male-sterile wheat with blue seeds subjected to back crossing for sowing in autumn in order to naturally eliminate non-anti-freezing spring (or partial spring) dwarf male-sterile wheat plant individuals with blue seeds, the progeny individuals showing high anti-freezing performance in the wintering period, of winter dwarf male-sterile wheat plants with blue seeds, are selected and subjected to back crossing again; the date of sowing in autumn is gradually advanced each year, so as to gradually improve the selection of the winter dwarf male-sterile wheat with blue seeds; after performing back crossing to reach 8th to 10th generations, the winter dwarf male-sterile wheat with blue seeds can be created and has winter or semi-winter genetic background as well as blue seeds, dwarf stalks and male sterile linkage markers. With the adoption of the method, the genetic background of winter dwarf male-sterile wheat with blue seeds can be transformed from spring or semi spring property to winter or semi winter property, which creates conditions for applying wheat in Huang-Huai River Valley and north winter wheat regions based on the heterosis.