22results about How to "Reduce breeding costs" patented technology

The invention discloses a group of molecular markers related to the oil yield of carya illinoensis and application of the molecular markers, and relates to the technical field of biology. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO. 1-2. The molecular marker disclosed by the invention can be used for marker assisted selection (MAS) and genome selection (GS) of the carya illinoensis, so that the genetic improvement progress of the variety of the carya illinoensis can be accelerated.

The invention relates to the technical field of molecular breeding, and discloses a molecular marker related to the LDL-C level of porcine serum, a detection reagent and a breeding method. The invention also discloses a primer group for identifying the molecular marker, and the primer group is used for amplifying the following SNP sites: SNP01, which are obtained on the basis of a pig reference genome Sscrofa11.1 and are positioned on 112bp, 008bp and 261bp of a third chromosome, and the genotype is A / G; sNP02 is located at 112bp, 008bp and 354 bp of a third chromosome, and the genotype of SNP02 is A / G; sNP03 is located at 112bp, 008bp and 583bp of the third chromosome, and the genotype of SNP03 is A / G. The primer group for identifying the molecular marker can quickly and effectively identify the LDL-C level of pig serum, and is used for assisting in breeding pig strains with low cholesterol or specific lipid metabolism characteristics.

This invention provides an Indel molecular marker closely related to the soluble sugar content of pepper fruit and its application, belonging to the technical field of nucleic acid-based assay methods. The nucleotide sequence of the Indel molecular marker is shown in SEQ ID NO.1 of the sequence listing; the Indel molecular marker is located at positions 5274716-5274727 of the full-length sequence of chromosome 3 of the pepper reference genome. Primers for amplifying the Indel molecular marker include a forward primer and a reverse primer. The nucleotide sequence of the forward primer is shown in SEQ ID NO.2 of the sequence listing, and the nucleotide sequence of the reverse primer is shown in SEQ ID NO.3 of the sequence listing. This molecular marker can be directly applied in early generations of breeding, avoiding the destructive sampling of chemical detection, significantly reducing breeding costs, effectively shortening the breeding cycle, and greatly improving the selection efficiency of molecular marker-assisted breeding in peppers.

The utility model discloses a kind of water culture devices for yunnan rhizoma polygonati breeding, it is related to yunnan rhizoma polygonati cultivation technical field, including culture rack, the culture rack is slidably connected with multiple water storage channels, and the water storage channel upper end is provided with culture plate;Multiple planting holes are evenly provided in the culture plate, and the culture plate upper end is connected with multiple fixed components, and fixed component is located planting hole outer end.The utility model design structure is reasonable, it can simultaneously carry different batches, different genotypes yunnan rhizoma polygonati breeding material, while independent water supply head, gate plate can individually adjust the water supply of each water storage channel, water supply frequency, realize the accurate management of "one tank one strategy", satisfy the individualized demand of different breeding material, to improve the success rate of breeding, can avoid the problem of "excessive supply" or "insufficient supply" caused by unified water supply, accurately match the demand of each growth stage of yunnan rhizoma polygonati, save water resource, nutrient solution and other consumables, reduce breeding cost.

The present application relates to the technical field of plant breeding, and particularly relates to a molecular marker and application thereof in breeding soybean varieties with high oil content and high protein content. The molecular marker is constructed based on a SNP site; based on the genome version number Wm82.a2.v1, the SNP site is located at the 8th chromosome of soybean at the 9020004th position, and the polymorphism is T / A. The application comprises: (1) predicting or detecting the oil content or protein content of soybean; (2) identifying or breeding soybean varieties with high oil content or protein content; (3) molecular marker assisted breeding of soybean; (4) improvement of soybean varieties related to oil content or protein content; (5) improvement of soybean germplasm resources. The molecular marker provided by the present application has the characteristics of double trait synergistic correlation, strong specificity, efficient detection process and the like, and can be widely applied to the scenes of molecular marker assisted breeding of soybean, rapid evaluation of germplasm resources and the like, and provides key technical support for breeding of soybean varieties with double optimal traits.

The invention relates to the technical field of molecular breeding of crops, in particular to a KASP molecular marker related to the size of soybean seeds and application of the KASP molecular marker. The molecular marker group comprises three SNP (Single Nucleotide Polymorphism) sites located in an intron region of a gene GmW82.04G097200 of a soybean T2T genome, wherein an SNP1 site is located at 8152017bp, and T / C nucleotide polymorphism exists; the SNP2 site is located at 8152443bp, and the A / G nucleotide polymorphism exists; the SNP3 site is located at 8153901bp, and C / G nucleotide polymorphism exists in the SNP3 site; alleles of the three SNP sites are combined to form three haplotypes corresponding to soybean seeds in size. The method has the advantages that three groups of specific KASP primer groups are provided, and genotypes of different soybeans are rapidly distinguished by virtue of PCR amplification and fluorescence signal detection, so that accurate screening of large-grain phenotype individuals is realized, the breeding period is effectively shortened, and the breeding input cost is reduced.

The present application relates to a kind of KASP molecular markers related to common pakchoi leaf purple and its application, by QTL-seq technology and genetic map location, the main effect QTL site related to leaf purple located on the 3rd chromosome of common pakchoi is found, and further developed the molecular marker closely linked to site, by the seed or seedling leaf extraction DNA of color unknown common pakchoi material, combined with the KASP primer of the present application carries out KASP molecular marker genotyping test, the color of common pakchoi can be predicted by the genotyping result of marker, without using artificial identification method, can obviously improve breeding efficiency, save breeding cost.

The application discloses application of a molecular marker related to a pig fattening period average daily feeding time trait, relates to the technical field of biology, and is characterized in that the application is any one of A1) to A4) below: A1) detecting or assisting in detecting a pig fattening period average daily feeding time; A2) identifying, assisting in identifying a pig fattening period average daily feeding time; A3) preparing a product for detecting or assisting in detecting a pig fattening period average daily feeding time; and A4) preparing a product for identifying, assisting in identifying a pig fattening period average daily feeding time. The application of the molecular marker related to the pig fattening period average daily feeding time trait discovers that a site of A / C at 270462812 of a chromosome 1 in a pig reference genome Sus_scrofa.Sscrofa11.1 has a significant influence on the pig fattening period average daily feeding time.

This invention relates to the field of molecular marker technology, specifically to molecular markers for identifying early-forming medicinal agarwood. Using these molecular markers, it is possible to identify agarwood with early-forming characteristics, which is of great significance for the identification of agarwood germplasm resources and molecular marker breeding.

The invention discloses an SNP molecular marker related to grass carp feed utilization rate and application thereof, and belongs to the technical field of aquatic animal molecular markers and aquatic genetic breeding. According to the invention, an SNP (Single Nucleotide Polymorphism) site obviously related to the feed utilization rate of the grass carp is screened out, the SNP molecular marker is positioned at the 51653399 site on the chromosome 2 of the grass carp, the polymorphic site of the SNP molecular marker is G / T, and when the genotype of the site is GG, the feed utilization rate of the grass carp is relatively high. The invention also discloses application of the SNP molecular marker, the amplification primer or the kit in breeding of grass carp with high feed utilization rate. The SNP molecular marker provided by the invention can be used for early breeding of grass carp with high feed utilization rate, greatly reduces the breeding workload, can significantly shorten the breeding time, accelerates the breeding process, improves the breeding efficiency, and reduces the breeding cost.

The application provides a SNP molecular marker related to the egg production of chickens and an application thereof, and belongs to the technical field of molecular genetics and animal breeding. The SNP molecular marker is located at the 1141th site of a chicken genome ERalpha gene cDNA sequence, corresponds to the 136th bp in the sequence shown in SEQ ID NO. 1, and the base at the site is C or T. The SNP molecular marker can be used for early breeding selection of the egg production of chickens, effectively improves the average egg production of chicken groups, reduces breeding costs, and provides a convenient technical means for establishing a molecular marker assisted breeding of the egg production selection of chickens.

This invention provides a specific primer set for identifying the sex of Eucommia ulmoides and its identification method, relating to the field of plant molecular identification technology. The specific primer set includes primers INV53, INV54, INV55, and INV56. The upstream primer of primer INV53 is shown in SEQ ID NO.1, and the downstream primer is shown in SEQ ID NO.2; the upstream primer of primer INV54 is shown in SEQ ID NO.3, and the downstream primer is shown in SEQ ID NO.4; the upstream primer of primer INV55 is shown in SEQ ID NO.5, and the downstream primer is shown in SEQ ID NO.6; the upstream primer of primer INV56 is shown in SEQ ID NO.7, and the downstream primer is shown in SEQ ID NO.8. The specific primer set for identifying the sex of Eucommia ulmoides provided by this invention targets the Chr10a inverted region, achieving 100% identification accuracy. It features high specificity and accuracy, is adaptable to samples with different genetic backgrounds and cultivation environments, and solves the problems of low accuracy and poor stability in existing methods.

The application discloses a dCAPS molecular marker for amplifying a SNP site of a QTL of wheat scab and application thereof in the technical field of molecular biology, is located on the long arm of chromosome 5B of a wheat Chinese spring reference sequence, and a primer pair comprises a forward primer and a reverse primer, and the nucleotide sequences of the forward primer and the reverse primer are shown as SEQ ID NO. 1-2. The molecular marker is closely linked to scab resistance in the application, has high diagnostic accuracy, and can be widely used in assisted breeding of wheat resistant to scab.

This invention discloses a specific DNA fragment, primers, identification method, and application for sex identification in yellowfin tuna. The specific DNA fragment has the following gene sequence: male as shown in SEQ ID NO.1 and female as shown in SEQ ID NO.2. Primers were designed based on the specific DNA fragment sequence for PCR amplification and Sanger sequencing analysis. The genotype at position 219 of the male yellowfin tuna DNA fragment is T / G heterozygous, and at position 292 it is A / G heterozygous. The genotype at position 219 of the female yellowfin tuna DNA fragment is T / T homozygous, and at position 292 it is A / A homozygous. This invention successfully achieves non-lethal sex identification during the fry or juvenile stage, which is beneficial for the implementation of asexual reproduction and seedling selection in yellowfin tuna, and has significant practical value and application potential.

The application discloses a SirB gene SNP molecular marker related to Dalbergia odorifera tree height and application thereof, and belongs to the technical field of genetic engineering. The SNP molecular marker disclosed by the application is located at the 290th base of the sequence shown in SEQ ID NO. 5, the polymorphism of the molecular marker is C or T, and the genotypes include CC, CT and TT. It is verified through correlation analysis that the tree height of the Dalbergia odorifera with the CC genotype is significantly higher than that of the individuals with the CT genotype and the TT genotype. The application further provides a kit for detecting the SNP molecular marker and application of the kit in the aspect of Dalbergia odorifera tree height trait auxiliary screening. With the aid of the molecular marker, the superior genotype individuals can be quickly and accurately identified at the Dalbergia odorifera seedling stage, the breeding efficiency of the Dalbergia odorifera can be significantly improved, and the application has important value for cultivating the Dalbergia odorifera with rapid growth.

This invention discloses a structural variation molecular marker on chromosome 5 of pigs that affects the weight of subcutaneous abdominal fat and its application. The structural variation molecular marker is a DNA fragment inserted after the 66120412 bp site on chromosome 5 corresponding to the International Pig Reference Genome Version 11.1; the nucleotide sequence of the DNA fragment is shown in SEQ ID NO:1. The structural variation molecular marker provided by this invention is significantly correlated with the trait of subcutaneous abdominal fat weight in pigs. By identifying this structural variation molecular marker, the trait of subcutaneous abdominal fat weight in pigs can be identified. Furthermore, by selecting individuals carrying the structural variation molecular marker during the breeding process, the subcutaneous abdominal fat weight of offspring pigs can be reduced, and the lean meat percentage of offspring pigs can be increased.

The invention discloses an SNP molecular marker related to grass carp feed utilization rate and application thereof, and belongs to the technical field of aquatic animal molecular markers and aquatic genetic breeding. According to the invention, an SNP (Single Nucleotide Polymorphism) site obviously related to the feed utilization rate of the grass carp is screened out, the SNP molecular marker is positioned at the 51653399 site on the chromosome 2 of the grass carp, the polymorphic site of the SNP molecular marker is G / T, and when the genotype of the site is GG, the feed utilization rate of the grass carp is relatively high. The invention also discloses application of the SNP molecular marker, the amplification primer or the kit in breeding of grass carp with high feed utilization rate. The SNP molecular marker provided by the invention can be used for early breeding of grass carp with high feed utilization rate, greatly reduces the breeding workload, can significantly shorten the breeding time, accelerates the breeding process, improves the breeding efficiency, and reduces the breeding cost.

The application discloses a kind of based on whole genome efficient prediction cassava tuber amylopectin content method, belong to plant molecular breeding technical field.The method includes the following steps: obtaining the whole genome SNP data of the measured cassava sample;The whole genome SNP data is substituted into prediction model, and the tuber amylopectin content of the measured cassava is predicted.The application establishes high-precision prediction model by integrating whole genome SNP marker data and machine learning algorithm, can accurately predict the tuber amylopectin content in cassava seedling stage, breaks through the technical bottleneck that must wait for cassava to determine in traditional breeding maturity, significantly shorten breeding cycle, reduce breeding cost, improve screening efficiency, provides efficient technical platform for cassava high amylopectin variety breeding.

This invention discloses a PtoSAUR74 gene related to the adventitious root formation ability of poplar and its applications. The nucleotide sequence of the coding region of the PtoSAUR74 gene disclosed in this invention is shown in SEQ ID NO.1, and the protein sequence is shown in SEQ ID NO.2. Overexpression of the PtoSAUR74 gene in poplar significantly inhibits the length, number, and growth rate of adventitious roots, indicating that the PtoSAUR74 gene is a key regulatory gene affecting the development of adventitious roots in poplar, providing a key target for precise molecular regulation of poplar root growth. This invention also discloses a method for regulating the adventitious root formation ability of poplar. By regulating the expression of the PtoSAUR74 gene, adventitious root formation can be directly inhibited, providing new technical means and theoretical basis for the genetic improvement of poplar.

This invention discloses a SNP molecular marker combination for breeding high-expansion-multiplicity popcorn and its application, belonging to the field of molecular breeding technology. The SNP molecular marker combination of this invention consists of 30 high-effect SNP loci located on the maize B73 reference genome v4 version. This combination was selected by performing genome-wide association analysis on 399 natural popcorn accessions and ranking the top 30 loci according to their SNP effect values. This invention allows for accurate prediction of the expansion-multiplicity trait by detecting the genotypes of these 30 SNP markers in the early stages of breeding, with a prediction accuracy of over 0.72. It has significant advantages such as high selection efficiency, short cycle, low cost, and insensitivity to environmental influences, and is suitable for molecular marker-assisted breeding and multi-trait aggregation breeding of popcorn.

The invention discloses a molecular marker closely linked with a rape pod length character and application of the molecular marker, and relates to the field of plant molecular biology. The molecular marker provided by the invention is C4-ID28, and comprises a forward primer and a reverse primer of which the nucleotide sequences are respectively shown as SEQ ID NO.1 and SEQ ID NO.2. Through identification, the primer of the molecular marker can be used for genotype identification in the early stage of breeding, and when the amplification result of the primer is a single electrophoretic band, the single plant is judged to be in a short-silique phenotype; and if the amplification result is two electrophoretic bands, determining that the single plant is in a long-silique phenotype. The primer can accurately screen the silique length phenotype, significantly improve the selection efficiency and reduce the field workload, thereby accelerating the breeding process.

The present application relates to SNP markers related to tobacco leaf number traits and application thereof. The present application mines single nucleotide polymorphism sites located in different chromosomes and gene regions, and the nucleotide variation forms include common SNP types such as A / T, A / G, C / T, C / G or G / T. These SNPs can be typed by conventional molecular detection methods. These sites all show significant and stable characteristic contribution values in the XGBoost model of the present application, can effectively reflect the genetic influence of leaf number, and can be used for rapid prediction of tobacco material leaf number, material screening and molecular marker assisted selection, so as to achieve early identification of target leaf number material, reduce breeding cost and accelerate new strain breeding.