8results about How to "Reduce breeding costs" patented technology

The utility model discloses a kind of water culture devices for yunnan rhizoma polygonati breeding, it is related to yunnan rhizoma polygonati cultivation technical field, including culture rack, the culture rack is slidably connected with multiple water storage channels, and the water storage channel upper end is provided with culture plate;Multiple planting holes are evenly provided in the culture plate, and the culture plate upper end is connected with multiple fixed components, and fixed component is located planting hole outer end.The utility model design structure is reasonable, it can simultaneously carry different batches, different genotypes yunnan rhizoma polygonati breeding material, while independent water supply head, gate plate can individually adjust the water supply of each water storage channel, water supply frequency, realize the accurate management of "one tank one strategy", satisfy the individualized demand of different breeding material, to improve the success rate of breeding, can avoid the problem of "excessive supply" or "insufficient supply" caused by unified water supply, accurately match the demand of each growth stage of yunnan rhizoma polygonati, save water resource, nutrient solution and other consumables, reduce breeding cost.

The present application relates to the technical field of plant breeding, and particularly relates to a molecular marker and application thereof in breeding soybean varieties with high oil content and high protein content. The molecular marker is constructed based on a SNP site; based on the genome version number Wm82.a2.v1, the SNP site is located at the 8th chromosome of soybean at the 9020004th position, and the polymorphism is T / A. The application comprises: (1) predicting or detecting the oil content or protein content of soybean; (2) identifying or breeding soybean varieties with high oil content or protein content; (3) molecular marker assisted breeding of soybean; (4) improvement of soybean varieties related to oil content or protein content; (5) improvement of soybean germplasm resources. The molecular marker provided by the present application has the characteristics of double trait synergistic correlation, strong specificity, efficient detection process and the like, and can be widely applied to the scenes of molecular marker assisted breeding of soybean, rapid evaluation of germplasm resources and the like, and provides key technical support for breeding of soybean varieties with double optimal traits.

This invention relates to the field of molecular marker technology, specifically to molecular markers for identifying early-forming medicinal agarwood. Using these molecular markers, it is possible to identify agarwood with early-forming characteristics, which is of great significance for the identification of agarwood germplasm resources and molecular marker breeding.

This invention provides a specific primer set for identifying the sex of Eucommia ulmoides and its identification method, relating to the field of plant molecular identification technology. The specific primer set includes primers INV53, INV54, INV55, and INV56. The upstream primer of primer INV53 is shown in SEQ ID NO.1, and the downstream primer is shown in SEQ ID NO.2; the upstream primer of primer INV54 is shown in SEQ ID NO.3, and the downstream primer is shown in SEQ ID NO.4; the upstream primer of primer INV55 is shown in SEQ ID NO.5, and the downstream primer is shown in SEQ ID NO.6; the upstream primer of primer INV56 is shown in SEQ ID NO.7, and the downstream primer is shown in SEQ ID NO.8. The specific primer set for identifying the sex of Eucommia ulmoides provided by this invention targets the Chr10a inverted region, achieving 100% identification accuracy. It features high specificity and accuracy, is adaptable to samples with different genetic backgrounds and cultivation environments, and solves the problems of low accuracy and poor stability in existing methods.

The application discloses a dCAPS molecular marker for amplifying a SNP site of a QTL of wheat scab and application thereof in the technical field of molecular biology, is located on the long arm of chromosome 5B of a wheat Chinese spring reference sequence, and a primer pair comprises a forward primer and a reverse primer, and the nucleotide sequences of the forward primer and the reverse primer are shown as SEQ ID NO. 1-2. The molecular marker is closely linked to scab resistance in the application, has high diagnostic accuracy, and can be widely used in assisted breeding of wheat resistant to scab.

The application discloses a kind of based on whole genome efficient prediction cassava tuber amylopectin content method, belong to plant molecular breeding technical field.The method includes the following steps: obtaining the whole genome SNP data of the measured cassava sample;The whole genome SNP data is substituted into prediction model, and the tuber amylopectin content of the measured cassava is predicted.The application establishes high-precision prediction model by integrating whole genome SNP marker data and machine learning algorithm, can accurately predict the tuber amylopectin content in cassava seedling stage, breaks through the technical bottleneck that must wait for cassava to determine in traditional breeding maturity, significantly shorten breeding cycle, reduce breeding cost, improve screening efficiency, provides efficient technical platform for cassava high amylopectin variety breeding.

This invention discloses a SNP molecular marker combination for breeding high-expansion-multiplicity popcorn and its application, belonging to the field of molecular breeding technology. The SNP molecular marker combination of this invention consists of 30 high-effect SNP loci located on the maize B73 reference genome v4 version. This combination was selected by performing genome-wide association analysis on 399 natural popcorn accessions and ranking the top 30 loci according to their SNP effect values. This invention allows for accurate prediction of the expansion-multiplicity trait by detecting the genotypes of these 30 SNP markers in the early stages of breeding, with a prediction accuracy of over 0.72. It has significant advantages such as high selection efficiency, short cycle, low cost, and insensitivity to environmental influences, and is suitable for molecular marker-assisted breeding and multi-trait aggregation breeding of popcorn.

The present application relates to SNP markers related to tobacco leaf number traits and application thereof. The present application mines single nucleotide polymorphism sites located in different chromosomes and gene regions, and the nucleotide variation forms include common SNP types such as A / T, A / G, C / T, C / G or G / T. These SNPs can be typed by conventional molecular detection methods. These sites all show significant and stable characteristic contribution values in the XGBoost model of the present application, can effectively reflect the genetic influence of leaf number, and can be used for rapid prediction of tobacco material leaf number, material screening and molecular marker assisted selection, so as to achieve early identification of target leaf number material, reduce breeding cost and accelerate new strain breeding.