Method for breeding rehmannia toxin-free seedling

A technology for Rehmannia glutinosa and seedlings, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as serious virus infection, quality degradation, and yield decline.

Inactive Publication Date: 2012-01-04
HENAN NORMAL UNIV
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Problems solved by technology

[0002] Rehmannia glutinosa is one of the famous "four Huai medicines" in my country and a traditional Chinese medicinal material. It occupies an extremely important position in Chinese herbal medicine. decline, quality degradation

Method used

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Embodiment Construction

[0004] The present invention is described in detail in conjunction with the following examples:

[0005] (1) For the production of first-class anti-virus seedlings of Rehmannia glutinosa, cut the anti-virus test tube seedlings of Rehmannia glutinosa with a length of about 1cm on the ultra-clean workbench and inoculate them with MS+NAA 0.01~0.05mg / L +6-BA0.05~0.5mg / L medium, cultured under the conditions of light intensity 2000lux, pH6.0 and temperature 25-30℃, after 30~40 days, the reproduction coefficient can reach about 5, and the grown offspring Toxic test-tube seedlings; on the ultra-clean workbench, cut the above-mentioned anti-virus test-tube seedlings of Rehmannia glutinosa into about 1cm long stems with axillary buds and inoculate them in 1 / 2MS+PPP 333 0.02~0.2mg / L medium, cultured under the conditions of the culture room, roots began to form in 7 days, and a large number of roots could be formed in 15~20 days, and rooted virus-free test-tube plantlets were cultivated...

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Abstract

The invention discloses a method for breeding a rehmannia toxin-free seedling. The method disclosed by the invention comprises the following steps: production of an original rehmannia seedling: cutting a rehmannia toxin-free test-tube plantlet into stem segments provided with axillary buds with the length of about 1cm on a clean bench, inoculating the stem segments onto a culture medium with the concentration of MS+NAA of being 0.01-0.05mg/L and the concentration of 6-BA of being 0.05-0.5mg/L, and culturing under the culture conditions of light intensity of 2000lux, pH of 6.0 and temperature at 25-30 DEG C to obtain a toxin-free test tube plantle, wherein, reproduction coefficient can reach about 5 after 30-40 days; cutting the rehmannia toxin-free test tube plantlet into the stem segments provided with the axillary buds with the length of about 1cm on the clean bench, inoculating the stem segments onto a culture medium with the concentration of 1/2MS+PPP<333> of being 0.02-0.2mg/L, and culturing in a culturing room to obtain a rooted toxin-free test tube plantlet, wherein, roots are formed within 7 days and a large number of roots are formed within 15-20 days; and transplanting the test tube plantlet into a greenhouse to carry out acclimation so as to finally obtain the toxin-free seedling, wherein, transplanting survival rate can reach up to more than 95%. The method disclosed by the invention has the advantages that cost for breeding the toxin-free seedling is low and yield is high.

Description

Technical field: [0001] The invention relates to a seedling breeding method, in particular to a breeding method of rehmannia glutinosa virus-free seedlings. Background technique: [0002] Rehmannia glutinosa is one of the famous "four Huai medicines" in my country and a traditional Chinese medicinal material. It occupies an extremely important position in Chinese herbal medicine. decline, quality degradation. Therefore, how to remove the virus that infects Rehmannia glutinosa, increase its yield, improve its quality, and find a breeding technology system that can quickly promote the planting of virus-free seedlings has become an important issue that needs to be solved urgently in production. Invention content: [0003] The purpose of the present invention is to provide a high-quality, high-yield and low-cost method for breeding virus-free seedlings of Rehmannia glutinosa. The technical scheme for realizing the object of the present invention is a breeding method for rehma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00
Inventor 李明军陈明霞张晓丽赵喜亭周彩云徐守真李敬敬
Owner HENAN NORMAL UNIV
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