A Molecular Marking Method for Sheep Wool Curl

A technology of molecular marking and curling degree, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as hindering hair follicle re-growth, and achieve the effect of reducing breeding costs, low detection condition requirements, and high efficiency

Inactive Publication Date: 2011-12-21
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
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Problems solved by technology

Specifically knocking out the DLX3 gene in hair follicle stem cells will lead to the disappearance of the BMP sig

Method used

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  • A Molecular Marking Method for Sheep Wool Curl
  • A Molecular Marking Method for Sheep Wool Curl
  • A Molecular Marking Method for Sheep Wool Curl

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0016] Specific embodiment 1: The molecular marking method of sheep wool curl degree is carried out according to the following steps:

[0017] 1. Using the phenol / chloroform method to extract sheep genomic DNA from the ear tissue of Chinese Merino sheep, design primers DLX3F1 and DLX3R1 based on the 228 position in the 3′UTR region of sheep DLX3 gene, and then perform PCR amplification on sheep genomic DNA to obtain The PCR amplified product is then digested with the restriction enzyme Hpy188I to obtain the digested product;

[0018] 2. Use an agarose gel with a concentration of 2% to 3% to separate the digested products by electrophoresis, and then determine the genotype according to the results of the electrophoresis separation. The criteria for determination: ①The electrophoresis shows two bands with sizes of 294bp and 214bp. Then there is no mutation at position 228 in the 3'UTR region of the sheep DLX3 gene, and the PCR amplified product can be completely cut by the restrictio...

Example Embodiment

[0041] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the PCR amplification reaction system in step 1 is a 10 μL reaction system, which consists of the following components:

[0042]

[0043] PCR amplification conditions were: pre-denaturation at 94°C for 5 minutes, denaturation at 94°C for 30s, annealing at 55°C for 30s, extension at 72°C for 30s, a total of 33 cycles, followed by extension at 72°C for 7 minutes and incubation at 4°C. Others are the same as the first embodiment.

Example Embodiment

[0044] Specific embodiment three: This embodiment is different from specific embodiment one in that the digestion system in step one is as follows:

[0045]

[0046] Enzyme digestion conditions: 37°C digestion overnight. Others are the same as the first embodiment.

[0047]

[0048]

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Abstract

The invention provides a molecular marking method for sheep wool curl, which relates to a molecular marking method, which comprises the following steps of: 1, extracting the sheep genome deoxyribonucleic acid (DNA) for carrying out polymerase chain reaction (PCR) amplification and then carrying out enzyme digestion to obtain enzyme digestion products; 2, carrying out electrophoresis separation onthe enzyme digestion products and carrying out genotype judgment according to the electrophoresis separation results; 3, carrying out association analysis and evaluating least square mean value of the property to obtain a result that the wool curl of individuals with the TT genotype type and TC genotype in the three genotypes is obviously higher than the wool curl of individuals with the CC genotype; and 4, dividing experiment groups into three types according to the result in the third step to complete the operation of the molecular marking method. The molecular marking method can be used for wool curl and fineness molecular marking auxiliary culture, the early period selection of the breeding sheep can be realized, and the sheep can be selected after the birth, so the breeding cost can be reduced, the breeding process of high-quality fine wool sheep is accelerated, the operation is simple, the accuracy is high, the efficiency is high, and the automatic detection can be realized.

Description

technical field [0001] The invention relates to a molecular marking method. Background technique [0002] Fine-wool sheep occupy an important position in my country's animal husbandry industry, and the main product of fine-wool sheep is wool. As an important textile raw material, fine wool has high economic value. The production of fine-wool sheep is not only related to the economic development and social stability of the producing areas, but also to the development of my country's wool spinning industry and the balance of import and export trade. With the increasing demand for wool at home and abroad, the cultivation of high-quality fine-wool sheep has become an urgent problem to be solved in the field of wool production and sheep breeding. At present, one of the main problems facing the production of fine-wool sheep in my country is the aging of domestic fine-wool sheep breeds and the lack of new breeds that meet the changing needs of the current market. Therefore it is...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 王宁李辉荣恩光
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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