Molecular marker affecting early-stage growth of goats, detection method and application thereof

A molecular marker, goat technology, applied in the field of molecular biology, can solve the problems of speeding up the breeding process of new goat breeds, low accuracy, slow genetic progress, etc.

Active Publication Date: 2017-03-15
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the defects of low accuracy of growth trait phenotype selection and slow genetic progress in goat breeding, and provide a method for selection of MC4R gene molecular markers for goat growth traits, which can be used for goat molecular breeding and can improve the selection of goat growth traits Accelerate the process of breeding new breeds of goats

Method used

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  • Molecular marker affecting early-stage growth of goats, detection method and application thereof
  • Molecular marker affecting early-stage growth of goats, detection method and application thereof
  • Molecular marker affecting early-stage growth of goats, detection method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, MC4R gene 502A / G molecular marker selection method of goat early growth

[0031] Materials and Methods:

[0032] (1) Flock

[0033] The 163 Boer goat samples were fed and managed by the Liuhe Animal Experiment Base of Jiangsu Academy of Agricultural Sciences, and the nutrition and management levels were consistent. The body weight data were provided by the Liuhe Animal Experiment Base of Jiangsu Academy of Agricultural Sciences.

[0034] (2) Method

[0035] 1. Sample collection

[0036] Ear tissue samples were collected from each individual, brought back to the laboratory in an ice bucket, and stored at -20°C.

[0037] 2. Extraction of DNA

[0038] DNA was extracted by conventional phenol and chloroform methods, and OD was determined by electrophoresis 260 / 280 Check DNA quality.

[0039] 3. Amplification of the sequence containing the 502 site of the MC4R gene

[0040] According to the sequence NM_001285591.1, use Primer Primer 5 to design a pai...

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Abstract

The invention discloses a molecular marker affecting early-stage growth of goats, a detection method and application thereof. The molecular marker affecting early-stage growth of goats is located at goat MC4R gene 502 locus and has A / G polymorphism, and the early-stage weight of goats of AA type is larger than that of goats of GG type. A method for screening rapid-growing goat strain in the early stage is characterized by including the steps of detecting polymorphism of the goat MC4R gene 502 locus and selecting AA type individuals to reserve seed stock. The method is capable of improving accuracy of goat growth trait selection and used for ultra early seed selection to accelerate new goat species culture.

Description

technical field [0001] The invention belongs to the field of molecular biology, and relates to a molecular marker that affects the early growth of goats, a detection method and an application thereof. Background technique [0002] Melanocortin receptor-4 (MC4R) is a kind of peptide substance secreted by the ventromedial nucleus of the hypothalamus of animals. It is one of the five subtypes of the melanocortin receptor family. It belongs to the G protein Coupled receptor superfamily, transmembrane neuroreceptors. In mammals, MC4R has the function of mediating Leptin protein, and is an important signaling molecule that regulates energy balance and energy homeostasis. MC4R plays a key role in the control of appetite and body weight homeostasis by binding to its endogenous ligands melanocortin hormone or agouti and agouti-related proteins. In 1993, Gantz et al. first cloned the human MC4R gene, and located it on human chromosome 18 by fluorescent labeling in situ hybridization...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 张俊曹少先钱勇李隐侠孟春花王慧利
Owner JIANGSU ACAD OF AGRI SCI
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