49 results about "Marker selection" patented technology

The invention belongs to the field of molecular genetics, and discloses a molecular marker method for anti-rice blast gene Pi-hk1 (t) of paddy. The method comprises the following steps of: (1) selecting a paddy sample, and extracting genome deoxyribonucleic acid (DNA) of the paddy sample; and (2) performing polymerase chain reaction (PCR) amplification on the genome DNA of the paddy sample by utilizing any one, two or three pairs of molecular marker primers in s01, in5 and in3, performing electrophoretic detection on a PCR amplification product, and if corresponding DNA fragments of molecularmarkers are amplified, indicating that the Pi-hk1 (t) gene exists. In the method, the selection efficiency of a single marker is over 99.92 percent, and the selection efficiency of any double markersis 100 percent. The resistance level of the gene on rice blast can be forecasted by detecting whether the paddy contains the gene or not by the molecular marker primers provided by the invention, so the selection efficiency of the rice blast resistance of the paddy is improved greatly, and the process for breeding for disease resistance is accelerated.

The invention relates to a multi-gene high rice blast resistance material breeding method adopting marker assisted selection, and belongs to the field of rice breeding. According to the method, specific molecular marker assisted selection of rice blast resistance genes pita and pi5 is adopted, field resistance identification is combined, and accordingly, a new disease-resistant variety can be bred effectively. The method comprises implementation modes as follows: 1, parents P1 and P2 containing the rice blast resistance genes pita and pi5 respectively are hybridized with a susceptible parent Liaoxing 1 to establish a resistant and susceptible offspring group; 2, extraction of DNA of single resistant and susceptible offspring, PCR (polymerase chain reaction) amplification and electrophoretic analysis of different markers are performed; 3, the parents P1 and P2, the susceptible variety Liaoxing 1 and the single plant of the hybridized offspring group are planted in a rice blast high incidence area Donggang, Dandong, and leaf blast and spike and stem blast resistance identification is performed at a seedling stage and a maturation stage respectively. In combination of the resistance gene marker selection, the new multi-gene rice blast resistance variety containing pita and pi5 is directionally improved and cultivated.

There is provided a marker selection program for selecting a marker for use in genetic diagnosis. In the program, analysis is carried out by using at least two specimen databases which respectively store data of specimens belonging different populations. By carrying out analysis without integrating all specimen data into single population, information on a minority population can be surely reflected to a gene search. Since the characteristics of each population can be reflected, high-accuracy diagnosing functions can be obtained, providing a practical diagnosing system.

The invention relates to a marker primer interlocked with a wheat powdery mildew resistance gene PmHNK54 and application thereof in molecular marker-assisted selection. The sequence of the marker primer on a genetic map is Xbarc5, PnHNK54 and Xgwm312, and the genetic distances between Xbarc5 and PnHNK54 and between Xgwm312 and PnHNK54 are respectively 5.0cM and 6.0cM. 10 mu l of PCR (Polymerase Chain Reaction) reaction system during SSR (Simple Sequence Repeat) marker selection comprises 1 mu l of 10*buffer, 0.2 mu L of 10nM / mu l dNTP, 0.2 mu l of 20u M / mu L primer, 0.1 mu l of 5U / mu lTaq enzyme, 8.1 mu l of deionized water and 0.4 mu l of 20 ng / mu l genome DNA. An amplification procedure comprises the following steps of: pre-denaturing at 94 DEG C for 3 min, denaturing at 94 DEG C for 1 min, renaturing at 55 or 60 DEG C for 1 min, extending at 72 DEG C for 2 min, and repeating for 40 cycles; extending at 72 DEG C for 10 min; and performing 6% denaturing polyacrylamide gel electrophoresis or 8% nondenaturing polyacrylamide gel electrophoresis on amplification products, observing and taking pictures after silver nitrate dyeing. The invention finds the position of the powdery mildew resistance gene PmHNK54 in wheat parent material Zheng 9754, has the advantages of strong marker specificity, high stability, cost saving and high selection efficiency and is applicable to large scale, high throughput and automation.

The present invention belongs to the molecular genetics field and relates to the molecular marker method of the major gene bph2 for brown planthopper resistance in the rice variety ASD7. Via genetic linkage analysis of the individual F2 plant genotype and the brown planthopper resistance level of each F2:3 family produced by hybridizing the insect-resistant variety ASD7 with insect-susceptible variety C418, the molecular markers RM463 and RM7102 of the major gene loci bph2 in the insect-resistant variety ASD7 are obtained. In the ASD7 / C418BC1 population the correct rate of selection by either marker is 91.2%. In the BC2 population, the correct rate of selection by RM463 is 91.2%, the correct rate of selection by RM7102 is 89.9%. Detection of this major gene loci in ASD7 and its derivatives via these molecular markers will increase the selection efficiency of brown planthopper resistant rices.

The invention discloses a method for selecting goose carcass trait microsatellite molecular marker and belongs to the technical field of poultry breeding. The method comprises the processes of: (1) designing specific primers of goose RY 36-site, wherein an upstream primer is 5'-TCCCAGCTTCACTCCTTTTC-3', and a downstream primer is 5'-GTGGTGTTCTTGCGGTGTAG-3'; (2) measuring goose carcass trait; (3) carrying out a PCR (polymerase chain reaction) amplification reaction; (4) carrying out polymorphism analysis of a PCR amplification product, and (5) selecting a superior goose parent individual body. According to the invention, goose carcass trait selection is carried out according to the genotype, the accuracy is high and the operation is simple. The carcass trait selection is begun at an earlier period of goose life; a generation interval is shortened; the breeding process of the goose is accelerated; the selection time can be shortened by about three months in each generation, and the method can be generalized and applied in the field of poultry breeding.

The present invention discloses a chicken dominant white feather gene identification. The present invention provides primers for identifying the chicken dominant white feather gene, specifically the primer pairs comprising two single-stranded DNA represented by a sequence 3 and a sequence 4 in the sequence table. Experiment results show that: with the application of the method of the present invention to identify the genotype (genotype II or genotype Ii or genotype ii) of the chicken dominant white feather gene, the advantages of simple operation, rapid detection, accurate result, low toxicity, broad application range and the like are provided. According to the present invention, the important significance is provided for the white feather chicken breeding, and the white feather character can be easily and effectively selected during the breeding practice so as to guide the molecular genetic marker selection of the chicken dominant white feather character.

The present inventors presumed that the sd1 gene was the C20 oxidase gene, and isolated and identified the rice counterpart of Arbidopsis C20 oxidase gene. As a result, it was revealed that the rice sd1 gene encodes a novel C20 oxidase. Further studies showed that mutation in this gene led to plant semidwarfing. Utilization of the plant sd1 gene is expected to increase the yield of plants, particularly useful agricultural crops and ornamental plants, to add aesthetic value to ornamental plants through dwarfing, and also to increase the yield and efficient breeding of dwarfed plants by marker selection.

The invention belongs to the technical field of preparation of a pig genetic marker, and particularly relates to a preparation method of a pig muscle pH value related molecular marker and application of the pig muscle pH value related molecular marker on auxiliary marker selection. The molecular marker is obtained by cloning a GYS1 gene promoter sequence, and the nucleotide sequence of the molecular marker is as shown in the sequence table SEQ ID NO: 1; and a C267-T267 basic group on the 267th basic group of a sequence as shown in the sequence table SEQ ID NO: 2 is mutated to cause MvaIPCR-RFLP polymorphism. The invention also discloses application of the preparation method for obtaining the molecular marker and a polymorphism detection method. The preparation method disclosed by the invention provides a new genetic marker for carrying out the auxiliary marker selection of a high-muscle quality pig.

The invention discloses a breeding method for high-resistance and grain-saving high-quality laying hens. A local breed and a dwarf breed of our country are taken as materials, and DW (Dwarf Gene) is introduced into the local breed of our country. A new multiline cross system for high-quality laying hens is established by using the conventional breeding methods combined with molecular assisted marker selection and through strain selection, cross matching and the like, and finally, a new high-resistance and grain-saving high-quality laying hens breed is bred. The technical problem that the cultivation method in the prior art can not realize grain saving and high yield simultaneously is solved.

The present invention recognizes that current clinical laboratory testing methods for multiparametric single cell analysis are limited to analysis of intact live cells, and are insufficient for identification of signaling activation profile defining certain cell types, including but not limited to neoplastic and immunologically activated cell subsets. One aspect of the present invention generally relates to marker selection in panels to include proteins routinely assessed in standard FCM, while preferably also incorporating markers for surface receptor proteins within activated signaling cascades. A further aspect of the present invention generally relates to panel design for the following indications in neoplastic and non-neoplastic clinical applications as examples of the technology: (a) identification of CML progenitor cell subsets in the setting of disease recurrence after treatment discontinuation or relapse due to treatment resistance, and (b) characterization of activated basophils to predict the severity of an allergic response. Another aspect of the present invention generally relates to methods to measure levels of surface and IC biomarkers in separate or combined assays for robust characterization of each or select cell compartment, and data analysis based on results from each or all method(s) used for optimal detection of the markers. A further aspect of the present invention generally relates to the identification and profiling of cell subpopulations based on analysis of surface markers including those associated with lineage and maturation of cell types and receptor proteins, and the corresponding IC phosphoproteins including those in activated signaling cascades to predict certain disease states or response to treatment.

A marker selection method includes: a marker generation step for generating a marker defining a position reference when recording data according to the modulation rule of the hologram recording / reproduction; a calculation step for calculating a correlation value indicating the correlation between a template image indicating a maker and each of a first verification image containing the marker shifted by a predetermined pixel and a second verification image containing data; and a selection step for selecting such a marker that the peak of the correlation value in the first verification image becomes greater than the peak of the correlation value in the second verification image. The selected marker is used as marker data in a marker generator in a recording processing circuit during recoding and as template data of the template matching processing circuit in the reproduction processing circuit during reproduction.

The invention belongs to the technical field of goat molecular marker preparation, and in particular relates to three goat MC1R deficient mutants and application thereof. Three deficient mutants are found in a goat MC1R and application thereof, two mutation sites F250V and G255D casing receptor defects both are located in a sixth transmembrane domain, and another mutation site C267W casing the receptor defects is located on a third external loop. The alpha-MSH ligand affinity of the three MC1R deficient mutants is lost, inducible and constitutive cAMP levels are significantly reduced, and inducible and constitutive p-ERK are significantly reduced. The goat MC1R deficient mutants can lead to the fact that a melanin production signal is blocked and the formation of black coat is affected. The molecular marker can be applied to assisted marker selection of goat wool color.

The invention discloses a genetic molecular marking combination for anti-disease stichopus japonicas breeding and application of the genetic molecular marking combination and belongs to the field of aquatic product genetic breeding. The genetic molecular marking combination consists of three SNP (Single Nucleotide Polymorphism) markers SNP88, SNP112 and SNP126 related to anti-vibrio splendidus infection of stichopus japonicas, and the three marked dominant genotypes are respectively SNP88 (CC), SNP112 (AA) and SNP126 (TT). A breeding method with the genetic markers comprises the following operation steps: (1) extracting parent DNA (Deoxyribonucleic Acid) and typing; (2) selecting parents; (3) performing filial generation seedling breeding. By adopting the genetic molecular marking combination, effective molecular markers for improving the economic property of anti-vibrio splendidus infection of stichopus japonicas are provided, the genetic markers are adopted for auxiliary marker selection of stichopus japonicas, anti-disease breeding can be carried out, the anti-vibrio splendidus infection of the stichopus japonicas can be improved, the morbidity of skin ulcer syndromes of the stichopus japonicas can be substantially reduced, and the combination has great significances in increasing the breeding survival rate and the breeding benefits.

The invention relates to a molecular marking method related to soybean hundred-grain weight, comprising the steps of 1) acquiring distribution of soybean RIL (recombinant inbred line) populations, tobe specific, subjecting advanced RIL populations of glossy-skin soybean and Qi Huang 26 to continuous two-year planting, and counting their hundred-grain weight; 2) performing QTL (quantitative traitloci) positioning on soybean hundred-grain weight, to be specific, using a high-density genetic map constructed via SLAF-seq (specific-locus amplified fragment sequencing) technique to perform QTL positioning on the hundred-grain weight of the soybean RIL populations so as to obtain 10 QTL related to the hundred-grain weight. Low-generation marker selection of breeding materials by the marker combination helps gather heavy-grain excellent genes in the breeding materials at low generation, advanced breeding selection work is reduced, breeding scale is narrowed, and breeding time is shortened.

The invention discloses a fast spectrum clustering method based on improved kd tree mark point selection. The invention firstly uses the improved kd tree method to select the marker points in the dataset, then establishes the similarity matrix between the data according to the marker points, and finally uses a depth self-coding instead of the traditional dimension reduction method for eigendecomposing the Laplace matrix. In the experiment, we prove the superiority of our method. In the large-scale data set, the algorithm improves the speed of clustering greatly with the accuracy basically consistent with the classical spectral clustering algorithm.

The invention relates to a fishy taste susceptible gene of a quail egg and application of the fishy taste susceptible gene as a molecular marker. A nucleotide sequence of the fishy taste susceptible gene FMO3 (Flavin Containing Monooxygenase3) is represented by SEQ ID NO: 1. A Q319X mutational site is served as an SNP (Single Nucleotide Polymorphism) molecular marker for detecting the fishy taste susceptible gene of the quail egg. The fishy taste susceptible gene of the quail egg, provided by the invention, is an auxiliary molecular marker of the quail egg with a fishy taste character, so that a molecular genetic marker selecting method with high efficiency is provided for accelerating the improvement of the flavor of the quail egg. The method greatly reduces the molecular breeding expenditure, effectively gets rid of the problem that the quail egg has fish taste in the production, enlarges the range of the selective feed raw materials of the quail, reduces the feeding cost, and improves the economic benefit of the quail breeding. The method is simple to operate, cheap in cost, short in detection time, high in accuracy, and capable of realizing automatic detection, thereby playing a great role the in quail breeding.

The invention discloses a primer for screening wheat leaf rust resistance QTL and an application of the primer, and relates to the technical field of determination or detection method of nucleic acid. The primer is a primer pair consisting of upstream and downstream nucleotide sequences of primers wmc692 and wmca657. The invention provides novel SSR markers wmc692 and wmc657 of the wheat leaf rust resistance QTL-(i) QLr.zh-4B. A molecular marker which is tightly linked to the QTL can be utilized to perform the assistant marker selection for the QTL, so that the accumulation of a plurality of active disease-resistant genes can be realized, and the disease resistance of a species can be prolonged. The novel leaf rust resistance QTL and the special primer can play an important role in the long-term disease resistance breeding of wheat.

The invention discloses a new method for lung adenocarcinoma biomarker screening, prognosis model construction and biological verification. The method comprises the following steps: 1, carrying out data preprocessing on an original gene expression matrix; 2, carrying out biomarker screening on the data obtained after preprocessing; 3, constructing a prognosis model by using the lung adenocarcinoma prognosis marker subjected to the multi-step feature selection; 4, carrying out signal analysis on the selected marker after modeling. The invention has the beneficial effects that 45 gene markers are verified by using a linear support vector machine model, AUC and ACC which are poorly verified by five folds are used as model evaluation indexes, and the final result is that AUC is equal to 0.98, and ACC is equal to 0.92. The result is obviously superior to that of most traditional gene marker selection methods, the marker related to the lung cancer can be found out, the lifetime of a lung cancer patient can be accurately predicted, meanwhile, a channel related to lung adenocarcinoma occurrence can be explored, and the occurrence mechanism of lung adenocarcinoma can be explored.

A marker selection method includes: a marker generation step for generating a marker defining a position reference when recording data according to the modulation rule of the hologram recording / reproduction; a calculation step for calculating a correlation value indicating the correlation between a template image indicating a maker and each of a first verification image containing the marker shifted by a predetermined pixel and a second verification image containing data; and a selection step for selecting such a marker that the peak of the correlation value in the first verification image becomes greater than the peak of the correlation value in the second verification image. The selected marker is used as marker data in a marker generator in a recording processing circuit during recoding and as template data of the template matching processing circuit in the reproduction processing circuit during reproduction.

The invention provides a cucurbita moschata transcriptome simple sequence repeat (SSR) molecular marker primer and application thereof. The cucurbita moschata transcriptome SSR molecular marker primercomprises 18 pairs of primer bodies, the sequences of the primer bodies are correspondingly shown in SEQ ID NO.1-36. A total of 6489 pairs of SSR primer bodies are designed by using Primer 3.0. Thirty pairs of primer bodies are randomly selected from 92 pairs of effective amplification primer bodies, and 30 cucurbita moschata germplasms are analyzed for polymorphism verification, wherein 18 pairs(account for 60%) of primer bodies show stable and repeatable polymorphism. UPGMA is used for mapping, and 30 selected materials are divided into 2 types. Cucurbita moschata transcriptome data are utilized to develop SSR markers to obtain high frequency SSR locus with rich types, and more abundant and reliable marker selection for genetic diversity analysis and genetic map construction in cucurbita moschata are provided.

The invention discloses a marker selection method for oil field logging. The method comprises the following steps of (a) selecting elements capable of participating in neutron activation treatment, and calculating element factors S of the selected elements according to the following formula (1): S=[theta]*[sigma]*[beta]*[gamma] / M(1); and (b) sorting the elements in a sequence from large S factors to small S factors according to the determination result in the step a, and primarily selecting the elements which are sorted in the front; then performing gamma ray interference elimination in the primarily selected elements, i.e., only remaining the elements with the large S factors in the elements with the characteristic gamma ray signal mutual interference; and regarding the finally left elements as marked elements. The invention provides a brand-new marker selection method; and through cooperation with the trace analysis, the flow rate of produced liquid in each target position under a well and the proportion of water to crude oil in the produced liquid can be accurately reduced. Meanwhile, the normal operation state of a horizontal well cannot be damaged, system errors can be effectively reduced, and the accuracy and reliability of the measurement result are improved.

The invention discloses a genetic molecular marking combination for anti-disease stichopus japonicas breeding and application of the genetic molecular marking combination and belongs to the field of aquatic product genetic breeding. The genetic molecular marking combination consists of three SNP (Single Nucleotide Polymorphism) markers SNP88, SNP112 and SNP126 related to anti-vibrio splendidus infection of stichopus japonicas, and the three marked dominant genotypes are respectively SNP88 (CC), SNP112 (AA) and SNP126 (TT). A breeding method with the genetic markers comprises the following operation steps: (1) extracting parent DNA (Deoxyribonucleic Acid) and typing; (2) selecting parents; (3) performing filial generation seedling breeding. By adopting the genetic molecular marking combination, effective molecular markers for improving the economic property of anti-vibrio splendidus infection of stichopus japonicas are provided, the genetic markers are adopted for auxiliary marker selection of stichopus japonicas, anti-disease breeding can be carried out, the anti-vibrio splendidus infection of the stichopus japonicas can be improved, the morbidity of skin ulcer syndromes of the stichopus japonicas can be substantially reduced, and the combination has great significances in increasing the breeding survival rate and the breeding benefits.

The invention relates to a molecular marking method for soybean bemisia tabaci resistance breeding. The molecular marking method comprises the following steps of 1, distribution of resistances to bemisia tabaci by soybean RIL groups, wherein high generation RIL groups such as huapidou and qihuang 26 are planted for 2 years continuously, the resistances to bemisia tabaci by the soybean RIL groups are identified, and the operation is repeated twice; the quadratic mean is analyzed; 2, QTL locating of the resistances to bemisia tabaci by soybeans, wherein a high-density genetic map built through an SLAF-seq technology is utilized to conduct QTL locating on the resistances to bemisia tabaci by the soybean RIL groups to obtain two QTL loci related to the resistances to bemisia tabaci by the soybeans. Through the marking combination, a breeding material is subjected to marking and selecting of the low generations, superior genes of the resistances to bemisia tabaci in the breeding material are easily aggregated in the low generations, the workload of breeding selection of high generations is lowered, the breeding scale is reduced, and the breeding age limit is shortened.

The present invention provides Chinese pumpkin transcriptome SSR molecular marker primers and applications thereof. The molecular marker primers comprise 18 pairs of primers, the sequences of which are respectively shown in SEQ ID NO.1-36. A total of 6489 pairs of SSR primers were designed using Primer 3.0. 30 pairs of primers were randomly selected from 92 pairs of effective amplification primers, and polymorphism analysis was carried out on 30 Chinese squash germplasms, among which 18 pairs (accounting for 60%) showed stable and reproducible polymorphisms. Using UPGMA to plot, the 30 test materials were divided into 2 categories. Using Chinese pumpkin transcriptome data for SSR marker development can obtain a higher frequency of SSR loci, and the types are rich, providing more abundant and reliable marker selection for the analysis of genetic diversity and genetic map construction of Chinese squash.

The invention provides a syzygium samarangense EST-SSR molecular marker, and belongs to the field of a molecular marker. By utilizing syzygium samarangense transcriptome data to carry out SSR marker development, a high-frequency SSR site can be obtained; 22 pairs of primers are determined; types are rich; richer and more reliable marker selection is provided for syzygium samarangense genetic diversity analysis and genetic map construction.

The invention belongs to the field of molecular marker assisted breeding of aquatic animals, and in particular relates to a group of SNP markers related to the growth of prawns and their application in breeding. The growth-related markers are shown in the sequences SNP3085_190487, SNP1678_860109, SNP3406_465799, and SNP2570_399791, and the dominant genotypes of the markers are CC, GG, GG, and CC, respectively. By genotyping one or more SNP markers and Marker selection can significantly increase the growth rate of prawns. The invention also discloses the application of a group of growth-related markers in genetic breeding. Compared with traditional breeding methods, the use of trait-related molecular marker-assisted breeding has the characteristics of accuracy and efficiency, and multiple markers provided by this patent can be used in combination to achieve rapid improvement of growth traits through marker aggregation, and in the cultivation of high-yielding varieties has broad application prospects.

The invention provides a syzygium samarangense EST-SSR molecular marker, and belongs to the field of a molecular marker. By utilizing syzygium samarangense transcriptome data to carry out SSR marker development, a high-frequency SSR site can be obtained; 22 pairs of primers are determined; types are rich; richer and more reliable marker selection is provided for syzygium samarangense genetic diversity analysis and genetic map construction.