7 results about "Marker selection" patented technology

The application provides a SNP molecular marker related to a multiple lamb trait of sheep, a detection primer set, a kit and application thereof, and belongs to the technical field of SNP molecular markers. The application provides application of the SNP molecular marker related to the multiple lamb trait of sheep in detection of the multiple lamb trait of sheep or sheep breeding. ABCA1 The application provides a method for detecting single nucleotide polymorphism of ABCA1 gene of sheep and application thereof, and provides basic data for molecular assisted marker selection breeding of sheep and accelerates improvement of Chinese sheep germplasm resources.

The application discloses application of a reagent for detecting a biomarker in preparation of a product for predicting ulcerative colitis disease activity or a product for diagnosing ulcerative colitis disease, and relates to the technical field of serological markers of inflammatory bowel disease. The serum CD105 of a UC patient is significantly higher than that of a healthy control group, and CD105 has a very high effect of diagnosing ulcerative colitis disease, and CD105 can distinguish disease activity from remission. Therefore, the application provides a novel serum marker which is convenient to sample, has good repeatability and can objectively reflect the colon mucosa inflammation load, and is expected to provide more accurate marker selection for UC disease activity prediction and UC diagnosis.

The application discloses a Holstein cow sperm activity miRNA molecular marker selection method, which comprises the following steps: (1) specific primers are designed according to a Holstein cow miRNA precursor region, and PCR amplification is carried out on to-be-detected cow genomic DNA to obtain an amplification product; (2) the PCR product is subjected to enzyme cutting by using a BspE I restriction endonuclease, and electrophoresis typing is carried out, and three genotypes, namely, a TT type, a TC type and a CC type, are obtained; and (3) the sperm activity of the CC type is significantly higher than that of the TT type, and the sperm deformity rate of the CC type is significantly lower than that of the TT type, so the CC type and the TC type are selected for breeding, and the C genotype frequency is improved. The method can improve the accuracy of sperm quality selection of the cow, is used for the selection and breeding of high-quality cows, and provides a selection and breeding tool for strengthening the excellent germplasm of Chinese Holstein cows.

This invention discloses a method for synergistic improvement of wheat quality and disease resistance based on the creation and utilization of the T1DL.1VS translocation line. Addressing the problem of low breeding efficiency caused by the dispersion of disease resistance and quality genes, this invention creates a novel T1DL.1VS translocation line that aggregates Pm67 / Glu-V1 by crossing the T1DL.1V#4S translocation line NAU195 carrying Glu-V1 with the T1DL.1V#5S translocation line NAU196 carrying Pm67. Recombinant single plants are screened in the F2 generation. Furthermore, utilizing the co-segregation characteristic of this translocation line with the wax repressor gene IW-V1, a dual-marker selection system combining morphological markers and the NAU1VS-1 molecular marker is established and introduced into a high-yielding variety background. This translocation line has no significant negative effect on yield traits, and the newly bred lines exhibit high resistance to powdery mildew and significantly improved bread processing quality, achieving synergistic and efficient improvement of wheat disease resistance and processing quality.

The invention provides a model hypothesis-independent survival analysis marker discovery method and device, and the method comprises the steps: collecting the follow-up survival data of a plurality of patients, and building a survival data set, samples of the data set comprise multi-dimensional features of biomarkers reflecting prognosis conditions of patients, actual observation time and labels indicating whether interested events are observed or not; constructing a model-free survival analysis marker selection optimization model, wherein the optimization model comprises a nucleation survival outcome dependency module for quantifying a dependency relationship between each feature and a survival outcome and a nucleation minimum redundancy module for eliminating redundant features; and solving the optimization model by utilizing the survival data set to obtain a screening result of the biomarker for predicting the prognosis condition of the patient. According to the method, the biomarker combination with high predictability and low redundancy can be effectively screened out under the condition that a preset model form is not needed, so that the discrimination capability and interpretability of the biomarker combination are improved.

The invention relates to the technical field of molecular genetic breeding, and discloses a soybean plant type gene cloning and multiple PCR (Polymerase Chain Reaction) rapid identification method and application, comprising a PCR detection method of a stem apical clustered pod material and a stem apical normal pod material, molecular markers for gene positioning, cloning and plant type identification, and a PCR specific reaction system and conditions. The detection method can be applied to the fields of variety resource identification, transgenic breeding, gene editing breeding, molecular marker selective breeding, gene function research and the like. The method has the advantages of economy, rapidness, simplicity in operation and the like.

The invention belongs to the technical field of development of vegetable disease-resistant molecular markers and molecular marker-assisted breeding, and particularly relates to development and application of a watermelon zucchini yellow mosaic virus-resistant molecular marker. The invention provides a molecular marker which is developed based on BSA (Bovine Serum Albumin) mixed pool positioning disease-resistant gene positioning and is used for identifying the resistance of the small-zucchini yellow mosaic virus in watermelons, and also provides application of the molecular marker, and the molecular marker is used for identifying the resistance germplasm of the small-zucchini yellow mosaic virus in the watermelons or assisting in selective breeding of offspring of the germplasm. The method is applicable to marker selection of most watermelons for resisting the zucchini yellow mosaic virus disease.