The invention relates to a multi-gene high rice blast resistance material breeding method adopting marker assisted selection, and belongs to the field of rice breeding. According to the method, specific molecular marker assisted selection of rice blast resistance genes pita and pi5 is adopted, field resistance identification is combined, and accordingly, a new disease-resistant variety can be bred effectively. The method comprises implementation modes as follows: 1, parents P1 and P2 containing the rice blast resistance genes pita and pi5 respectively are hybridized with a susceptible parent Liaoxing 1 to establish a resistant and susceptible offspring group; 2, extraction of DNA of single resistant and susceptible offspring, PCR (polymerase chain reaction) amplification and electrophoretic analysis of different markers are performed; 3, the parents P1 and P2, the susceptible variety Liaoxing 1 and the single plant of the hybridized offspring group are planted in a rice blast high incidence area Donggang, Dandong, and leaf blast and spike and stem blast resistance identification is performed at a seedling stage and a maturation stage respectively. In combination of the resistance gene marker selection, the new multi-gene rice blast resistance variety containing pita and pi5 is directionally improved and cultivated.