4results about How to "Accelerate cultivation" patented technology

This invention provides a gene related to loquat flowering. SVP1 This invention relates to the field of molecular biology, specifically to a tightly linked SNP marker and its applications. The nucleotide sequence of this SNP marker is shown in SEQ ID NO.1. A genotype of C at the locus corresponds to a spring-flowering phenotype in loquat, while a genotype of G corresponds to an autumn-flowering phenotype. The invention also provides specific primers (SEQ ID NO.2 and SEQ ID NO.3) for detecting this SNP marker, a kit containing these primers, and a screening method for detecting loquat flowering time using this marker. By extracting loquat DNA and performing PCR amplification and sequencing analysis, the flowering period of loquat can be quickly and accurately determined. The SNP marker of this invention is highly linked to loquat flowering time, providing an effective tool for marker-assisted selection breeding of loquat at different flowering periods. This significantly improves breeding efficiency, shortens the breeding cycle, and is of great significance for extending the loquat fruit supply period and optimizing the industrial structure.

The application discloses a SNP molecular marker related to the content of oleic acid in sesame seeds and application, and belongs to the technical field of molecular marker breeding. The SNP molecular marker related to the content of oleic acid in sesame seeds is located at 2754308 bp of the No. 3 chromosome, the genotype GG corresponds to the content of oleic acid which is significantly higher than that of AA (the difference reaches the extremely significant level of 0.01), and the accurate differentiation of high / low oleic acid germplasm can be realized. Early genotype identification can be carried out at the seedling stage of sesame by using the marker, and the breeding cycle is greatly shortened and the selection efficiency is improved without waiting for seed maturation. The marker is stable and reliable under different genetic backgrounds, is less affected by the environment, and is simple, high-flux and low-cost in detection, is suitable for large-scale automatic screening, can be directly used for molecular marker assisted selection of high-oleic-acid sesame varieties, accelerates the cultivation of high-quality new varieties, and has important application value for improving the sesame oil quality and market competitiveness.

The invention discloses a special fertilizer for promoting blossom, protecting fruits, increasing yield and improving quality of anise and a preparation method thereof, and belongs to the field of organic fertilizers. The formula comprises the following components in parts by mass: 500 parts of compound fertilizer, 20 parts of borax, 200 parts of medium trace element fertilizer, 40 parts of peanut bran, 360 parts of dried pig manure, 40 parts of fishbone dust, 230 parts of mushroom residues and 4 parts of microbial flora. The microorganism bacterium is a bacillus stearothermophilus fermentation bacterium agent; the nutrient content of the biological organic fertilizer is not less than 15%, and the organic matter content is not less than 40%. The borax and the medium trace elements in the formula have a synergistic effect and are matched with the microbial flora to promote nutrient absorption, so that anise bud cultivation can be effectively promoted, the flowering amount and the fruit setting rate are increased, leaves and growing branches of anise after fertilization are increased, the leaf color is dark green, and flower and fruit falling phenomena are obviously reduced. The high organic matter content can improve the soil hardening condition and improve the soil breathability and the water and fertilizer retention capacity, the microbial flora adjusts the soil micro-ecology, the soil fertility can be continuously improved after long-term application, and healthy growth of anise trees is promoted.

The present application relates to SNP markers related to tobacco leaf number traits and application thereof. The present application mines single nucleotide polymorphism sites located in different chromosomes and gene regions, and the nucleotide variation forms include common SNP types such as A / T, A / G, C / T, C / G or G / T. These SNPs can be typed by conventional molecular detection methods. These sites all show significant and stable characteristic contribution values in the XGBoost model of the present application, can effectively reflect the genetic influence of leaf number, and can be used for rapid prediction of tobacco material leaf number, material screening and molecular marker assisted selection, so as to achieve early identification of target leaf number material, reduce breeding cost and accelerate new strain breeding.