15results about How to "Accelerate cultivation" patented technology

The invention relates to the technical field of gene engineering, and discloses a KASP marker related to thousand seed weight of wheat and application of the KASP marker. The SNP locus is located at the 33397938bp position of the wheat 1A chromosome, and the polymorphism is C / T. Through phenotypic difference and exon capture analysis, the SNP site closely related to the thousand seed weight is determined, and a specific KASP primer combination is designed, so that rapid and accurate identification of the wheat thousand seed weight is realized. The application of the technology has significant significance in accelerating high-yield breeding of wheat. According to the method, the selection efficiency is improved, the breeding period is shortened, and wheat varieties with ideal thousand seed weight can be quickly identified and cultivated. By using the KASP primer combination, an effective tool is provided for molecular marker-assisted selection of wheat yield-related traits, and the overall yield and quality of crops can be improved.

The invention discloses a biological fermentation tank for medical strain cultivation, and relates to the technical field of liquid strain cultivation, the biological fermentation tank comprises a fermentation tank main body and a controller, the fermentation tank main body comprises a tank body and a storage bin, the tank body is installed above the storage bin, a stirring tank is installed in the tank body, a fermentation bin is arranged at the bottom of the tank body, and the controller is connected with the stirring tank. A collection assembly is installed in the storage area and matched with the fermentation bin, the stirring tank can accelerate strain mixing, the fermentation bin can accelerate strain cultivation, and the collection assembly can collect strains, so that when the device is used, nutrient liquid and the strains can be fully stirred, and the nutrient liquid and the strains can be uniformly mixed. Compared with an existing shaking machine, the shaking machine has the advantages that when the strains are shaken, the strains can fully absorb nutrients in nutrient liquid in the shaking process, so that the strains can be quickly cultivated in the subsequent cultivation process, and meanwhile, the survival rate of the cultivated strains is improved to a certain degree.

The present invention relates to the technical field of molecular markers, and in particular to SNP molecular markers linked to carotenoid content in tetraploid roses and applications thereof. A molecular marker closely linked to the formation and accumulation of rose carotenoids is obtained, and the nucleotide sequence of the SNP marker is shown in SEQ ID NO.1; the polymorphic site is located at the 101st position of the sequence shown in SEQ ID NO.1, and the polymorphism is A / G. According to the molecular marker sequence information, the target trait can be screened in the seedling stage, effectively shortening the traditional hybrid breeding years, and achieving accurate and efficient molecular breeding of rose color. Through molecular marker-assisted breeding, the carotenoid content in rose petals can be effectively increased, the cultivation of new yellow / orange rose varieties can be promoted, and natural derivative products such as plant essential oils and new food raw materials that meet public demand and have market potential can be developed, and the efficient transformation of multiple tissues of flowers and the innovative application of diversified products can be promoted.

The present invention relates to the field of molecular marker technology, and in particular to a tetraploid rose anthocyanin content-linked SNP molecular marker and its application. The present invention relates to a SNP marker related to the anthocyanin content of rose petals, wherein the SNP marker contains a nucleotide sequence as shown in SEQ ID NO.5, and the polymorphism is T / C. The target trait can be screened at the seedling stage, effectively shortening the traditional hybrid breeding period, and achieving accurate and efficient rose flower color molecular breeding. Through molecular marker-assisted breeding, the anthocyanin content in rose petals can be effectively increased, the cultivation of pink / red innovative rose varieties can be promoted, and natural derivative products such as plant essential oils and new food raw materials that meet public demand and have market potential can be developed, promoting the efficient transformation of multiple tissues of flowers and the innovative application of diversified products.

The invention discloses a soapberry SmSEP3 gene and application thereof, the gene is expressed in a plant to regulate and control the flowering time of the plant and promote pistil development, sepal curling and pod development, and the gene is a nucleotide sequence as shown in a sequence table Seq ID NO.1 or a nucleotide sequence of an amino acid sequence as shown in a coding sequence table Seq ID NO.2. The gene is used for cultivating new soapberry varieties with different flowering times, different flower types and larger fruits. Through the analysis of transgenic arabidopsis thaliana, the bolting and flowering time of the plant can be advanced through the overexpression of the arabidopsis thaliana, the pistil is obviously lengthened, sepals are curled, and the fruit volume is larger. The flowering time, bolting days and rosette leaf number of the transgenic arabidopsis thaliana plant are obviously different from those of a wild plant, pistil development is longer, fruit pods are increased, the breeding cycle of the sapindus tree species is accelerated, ornamental varieties of different flower types are cultivated, and cultivation of high-yield new varieties is accelerated.

This invention provides a gene related to loquat flowering. SVP1 This invention relates to the field of molecular biology, specifically to a tightly linked SNP marker and its applications. The nucleotide sequence of this SNP marker is shown in SEQ ID NO.1. A genotype of C at the locus corresponds to a spring-flowering phenotype in loquat, while a genotype of G corresponds to an autumn-flowering phenotype. The invention also provides specific primers (SEQ ID NO.2 and SEQ ID NO.3) for detecting this SNP marker, a kit containing these primers, and a screening method for detecting loquat flowering time using this marker. By extracting loquat DNA and performing PCR amplification and sequencing analysis, the flowering period of loquat can be quickly and accurately determined. The SNP marker of this invention is highly linked to loquat flowering time, providing an effective tool for marker-assisted selection breeding of loquat at different flowering periods. This significantly improves breeding efficiency, shortens the breeding cycle, and is of great significance for extending the loquat fruit supply period and optimizing the industrial structure.

The application discloses a SNP molecular marker related to the content of oleic acid in sesame seeds and application, and belongs to the technical field of molecular marker breeding. The SNP molecular marker related to the content of oleic acid in sesame seeds is located at 2754308 bp of the No. 3 chromosome, the genotype GG corresponds to the content of oleic acid which is significantly higher than that of AA (the difference reaches the extremely significant level of 0.01), and the accurate differentiation of high / low oleic acid germplasm can be realized. Early genotype identification can be carried out at the seedling stage of sesame by using the marker, and the breeding cycle is greatly shortened and the selection efficiency is improved without waiting for seed maturation. The marker is stable and reliable under different genetic backgrounds, is less affected by the environment, and is simple, high-flux and low-cost in detection, is suitable for large-scale automatic screening, can be directly used for molecular marker assisted selection of high-oleic-acid sesame varieties, accelerates the cultivation of high-quality new varieties, and has important application value for improving the sesame oil quality and market competitiveness.

The invention discloses a molecular genetic marker YZU-GOOSE-FC-2 for a deep and shallow feather color character of a goose and an application of the molecular genetic marker YZU-GOOSE-FC-2. The molecular genetic marker comprises an insertion molecular marker or a deletion molecular marker; the insertion molecular marker is an insertion base sequence AACT located between the G.126358 site and the G.126359 site of a gene where the molecular genetic marker YZU-GOOSE-FC-2 is located; the deletion molecular marker is a base sequence located between the G.126611 site and the G.126621 site of a gene where the molecular genetic marker YZU-GOOSE-FC-2 is located. According to the method, the accuracy of individual selection of the geese can be improved, the breeding population scale is reduced, the breeding cost is greatly saved, the breeding time is shortened, the breeding cost is reduced, and the breeding of the deep and shallow yellow feather auto-sexing commercial line is accelerated; the cost of gosling sex determination is reduced, and the disease transmission risk caused by sex determination through manual anal turning is reduced.

The invention discloses a special fertilizer for promoting blossom, protecting fruits, increasing yield and improving quality of anise and a preparation method thereof, and belongs to the field of organic fertilizers. The formula comprises the following components in parts by mass: 500 parts of compound fertilizer, 20 parts of borax, 200 parts of medium trace element fertilizer, 40 parts of peanut bran, 360 parts of dried pig manure, 40 parts of fishbone dust, 230 parts of mushroom residues and 4 parts of microbial flora. The microorganism bacterium is a bacillus stearothermophilus fermentation bacterium agent; the nutrient content of the biological organic fertilizer is not less than 15%, and the organic matter content is not less than 40%. The borax and the medium trace elements in the formula have a synergistic effect and are matched with the microbial flora to promote nutrient absorption, so that anise bud cultivation can be effectively promoted, the flowering amount and the fruit setting rate are increased, leaves and growing branches of anise after fertilization are increased, the leaf color is dark green, and flower and fruit falling phenomena are obviously reduced. The high organic matter content can improve the soil hardening condition and improve the soil breathability and the water and fertilizer retention capacity, the microbial flora adjusts the soil micro-ecology, the soil fertility can be continuously improved after long-term application, and healthy growth of anise trees is promoted.

The invention discloses a marker for efficiently identifying alfalfa chromosomes and application thereof, belongs to the field of bioinformatics and molecular cytogenetics, and solves the problems that alfalfa is high in ploidy, large in number of chromosomes, small in chromosomes, lack of obvious symbolic characteristics and high in identification difficulty. The marker for efficiently identifying the alfalfa chromosome is used for identifying chromosomes 1-8 of alfalfa, the marker of the alfalfa chromosome is located on the chromosomes 1-8 of the alfalfa, and the marker of the alfalfa chromosome is composed of six pairs of alfalfa specific sequence amplification primers; the marker for efficiently identifying the alfalfa chromosomes can efficiently and accurately identify the 1-8 chromosomes of the alfalfa, can identify the 1-8 chromosomes in the alfalfa at one time, can quickly distinguish different chromosomes, greatly shortens the identification time, improves the working efficiency, is beneficial to understanding the genetic diversity of the alfalfa, and has good application prospects. Individuals with excellent characters can be conveniently selected for hybridization.

The invention discloses an InDel molecular marker closely linked with the character of the oil content of peanuts, a primer pair for detecting the molecular marker and application, and belongs to the technical field of molecular marker-assisted breeding. According to the invention, the InDel is marked as Arahy36753720; the nucleotide sequence of the Arahy36753720 molecular marker is as shown in SEQ ID NO: 1 (Sequence Identity Number 1). The method for predicting the oil content of the peanuts by detecting the molecular marker has the advantages of rapidness, simplicity, convenience and high selection efficiency, can be directly realized through PCR amplification and electrophoresis, and has important application value in predicting the oil content of the peanuts and breeding high-oil-content peanuts.

The present application relates to SNP markers related to tobacco leaf number traits and application thereof. The present application mines single nucleotide polymorphism sites located in different chromosomes and gene regions, and the nucleotide variation forms include common SNP types such as A / T, A / G, C / T, C / G or G / T. These SNPs can be typed by conventional molecular detection methods. These sites all show significant and stable characteristic contribution values in the XGBoost model of the present application, can effectively reflect the genetic influence of leaf number, and can be used for rapid prediction of tobacco material leaf number, material screening and molecular marker assisted selection, so as to achieve early identification of target leaf number material, reduce breeding cost and accelerate new strain breeding.

The utility model discloses a soilless seedling raising box for agricultural technology, which comprises a main body, a heating device is fixedly connected in the main body, a working groove is arranged in the main body, and the working groove is positioned above the heating device and is suitable for keeping the best state of a nutrient solution; four pieces of heat preservation cotton are arranged, and the heat preservation cotton is suitable for assisting heat preservation work on the nutrient solution and the seedlings; the detection port is located outside the main body and is suitable for providing convenience for workers to inspect the nutrient solution; the main body, the heating device, the working groove, the heat preservation cotton, the water suction pump, the conveying water pipe, the water mist spraying device and the illumination lamp are matched for use, so that the device can keep a nutrient solution at a constant temperature, the nutrient solution can be kept in an optimal temperature state in cold weather, and the nutrient solution is prevented from being damaged. Therefore, the use effect of the nutrient solution is improved, the cultivation of seedlings is enhanced, and the practicability of the device is improved.

The invention provides a molecular marker for identifying tartary buckwheat grain width, a primer group for identifying the molecular marker and application of the molecular marker. On the basis of the molecular marker, a KASP technology is utilized, and through simple experimental steps, a sample can be identified, tartary buckwheat germplasm resources with different grain widths can be rapidly and efficiently screened, the breeding efficiency of wide-grain tartary buckwheat germplasm is improved, and cultivation of large-grain high-yield tartary buckwheat varieties is accelerated.

The invention discloses an SNP (Single Nucleotide Polymorphism) site for identifying a wheat stem rot resistant gene and application thereof. The invention aims to solve the problem of how to quickly and accurately identify or assist in identifying the basal stem rot resistance character of wheat. According to the application, a substance for detecting the polymorphism or genotype of an SNP site in a wheat genome is applied to identification or auxiliary identification of grain traits, the SNP site is located in a wheat 3AL chromosome, a KASP marker closely linked with the SNP site is Kasp3ALFCR, and the nucleotide type is A or G and is the 51st nucleotide of a sequence 1 in a sequence table. The gene type of the marker Kasp3ALFCR closely linked with the QTL in an amplification product can be identified, the marker can be used for predicting the basal stem rot resistance character of wheat, early screening of wheat can be carried out, and the marker can also be used for wheat molecular marker-assisted breeding.