128 results about "Bolting" patented technology

The present invention relates to a Spinacia oleracea seed designated 51-335 RZ, which exhibits late bolting, savoy mature leaves with an ovate shape, and resistance to downy mildew (Peronospora farinosa f.sp. spinaciae) races Pfs1 to Pfs13 and strain UA4410. The present invention also relates to a Spinacia oleracea plant produced by growing the 51-335 RZ seed. The invention further relates to methods for producing the spinach cultivar, represented by spinach variety 51-335 RZ.

The invention provides a cultivation method capable of preventing Angelica sinensis from premature bolting. The cultivation method comprises the following steps: deeply plowing soil and fertilizing at a planting area; sowing when the temperature of soil layer of 5 cm below the ground surface is 6-8 DEG C, wherein the row spacing is 20-25 cm, opening shallow gullies with the depth of 2-3 cm, after sowing, covering wet soil, and then covering wheatgrass, wherein the seedling density per hectare is 0.45-0.75 million plants, and the plant spacing is 5-10 cm; when the height of seedling is 3-4 cm, gapping and establishing so as to keep the plant spacing and the seedling density; sowing in late October of the present year, digging and sunning when the aerial part is withered so as to obtain the Angelica sinensis. According to the cultivation method, under the precondition of ensuring the yield and quality of Angelica sinensis, the Angelica sinensis is prevented from premature bolting, the seedling and transplantation procedures of Angelica sinensis are omitted, the production period of Angelica sinensis is shortened, 25% of seedling and seedling storage land is saved, and 120-130 days of production period of Angelica sinensis are shortened.

The invention discloses an FLC1 specific SNP molecular marker based on the competitive allele specificity PCR technology and an application, and provides an application of polymorphism or genotype matter for detecting FLC1-13859895G/ASNP locuses in a Chinese cabbage genome in authenticating or assisting in authenticating the bolting state of Chinese cabbages. The experiment proves that a developed SNP marker specific primer can well classify bolting characters, high application value is achieved, and preselection and assistant breeding of bolting resisting genetic materials can be achieved.

The invention discloses a spring Chinese cabbage, summer-autumn eggplant and autumn-winter asparagus lettuce three-season-in-one-year anti-season vegetable efficient cultivation method. In a gap date, first-season spring Chinese cabbages are sown in advance from the first ten-day period of February to the beginning of March, a setting date ranges from the first ten-day period of March to the beginning of April, and the first-season spring Chinese cabbages come into the market from the end of April to the middle ten-day period of May. Second-season summer-autumn eggplants are sown from the last ten-day period of March to the end of March, a setting date ranges from the last ten-day period of May to the end of May, and the second-season summer-autumn eggplants are harvested from the last ten-day period of July to the last ten-day period of September. Third-season autumn-winter asparagus lettuces are sown from the end of August to the first ten-day period of September, a setting date ranges from the end of September to the first ten-day period of October, and third-season autumn-winter asparagus lettuces are harvested from the last ten-day period of December to the beginning of January. Due to the fact that bolting-resistant varieties and the gap date are scientifically selected, a sowing data, the setting data and a seedling cultivation mode are improved, the early bolting problem of the spring Chinese cabbages is solved, three kinds of vegetables can come into the market in a slack season in one year in an anti-season mode, the demands of consumers are met, and the economic benefit is very remarkable.

The present invention relates to a Spinacia oleracea seed designated 51-332 RZ, which exhibits medium bolting, an erect plant habit, smooth mature leaves with a three-sided shape and resistance to downy mildew (Peronospora farinosa f. sp. spinaciae) races Pfs1 to Pfs13 and strain UA4410. The present invention also relates to a Spinacia oleracea plant produced by growing the 51-332 RZ seed. The invention further relates to methods for producing the spinach cultivar, represented by spinach variety 51-332 RZ.

The invention relates to an aquaculture method of deep sea scallops, in particular to a breeding and propagation method for Atlantic deep sea scallops. The method comprises the steps that introduction, artificial breeding and aquaculture of the deep sea scallops are carried out, parent scallops are mature after 45-50 days maturity promoting and lay eggs, and after most larvae grow into D-shaped larvae, a bolting cloth net cage is used for selecting optimal larvae to a new pond for cultivation; the growth rate of the cultured larvae is far higher than that of the larvae cultured in a nursery ground in Quebec of Canada at the same time, eyespots are generated on most larvae 16-17 days after the larvae are fertilized, adhering substances are arranged at the 18th day, after the adhering substances are arranged for 10 days, the adhering substances are taken out and hung in natural sea ears for larva protection; the deep sea scallops meet commercial specification when the cultivated average specification reaches 8 centimeters, and the deep sea scallops achieve sexual maturity. According to the method, the Atlantic deep sea scallops are introduced into China sea areas for the first time, the scallop culture species are expanded, and construction of modern ocean ranches is promoted.

The present invention relates to a Spinacia oleracea seed designated 51-707 RZ, which exhibits early bolting, erect plant habit, semi-savoy leaves, and resistance against downy mildew (Peronospora farinosa f. sp. spinaciae) races Pfs1 to Pfs13 and strain UA4410. The present invention also relates to a Spinacia oleracea plant produced by growing the 51-707 RZ seed. The invention further relates to methods for producing the spinach cultivar, represented by spinach variety 51-707 RZ.

The present invention relates to a Spinacia oleracea seed designated 51-333 RZ, which exhibits medium bolting, an erect plant habit, ovate first foliage leaves, semi-savoy mature leaves and resistance to downy mildew (Peronospora farinosa f. sp. spinaciae) races Pfs1 to Pfs13 and strain UA4410. The present invention also relates to a Spinacia oleracea plant produced by growing the 51-333 RZ seed. The invention further relates to methods for producing the spinach cultivar, represented by spinach variety 51-333 RZ.

The invention discloses a paddy rice NRT 1.1A gene and an application of encoded protein of the paddy rice NRT 1.1A gene to improve the plant yield in breeding. Concretely, protein or an encoded genethereof is applied to regulate the growth and development of a plant. The growth and development is reflected as the single plant yield and/or the strain height and/or grain number and/or flowering time and/or bolting time and/or size of a rosette leaf. The protein is any one of (1) protein having the amino acid sequence shown as in the sequence 1 in a sequence list; and (2) protein which is derived from the sequence 1 by replacing and/or deleting and/or adding one or more amino acid residues in and/or from and/or to the amino acid sequence in the sequence 1 in a sequence list, and is relatedto control of the growth and development of a plant. The paddy rice NRT 1.1A gene and encoded protein are of great importance in breeding of new high-yield paddy rice variety.

The invention discloses an application of soybean E3 ubiquitin ligase gene GmPUB2 capable of regulating flowering of plants, namely, the application of the oybean E3 ubiquitin ligase gene GmPUB2 in regulating flowering of plants. A late-flowering plant is obtained after the GmPUB2 gene is transplanted into a target plant through a plant expression vector; an early-flowering plant is obtained after the GmPUB2 gene is transplanted into a target plant through a gene silencing carrier; after the target gene GmPUB2 is transplanted into arabidopsis thaliana, compared with the wild arabidopsis thaliana, the results show that the bolting time and the flowering time of the transgenic plant are obviously later than those of the wild plant, namely, the GmPUB2 gene has the function of delaying the flowering of the plants. With the established GmPUB2 gene silencing carrier to silence the transgenic arabidopsis thaliana with over-expressed target gene GmPUB2, the results show that the flowering time of the plant after silencing is obviously earlier than that of a control plant.

The invention discloses a high-yield planting method of mustards in established Italian poplar forests. The method is characterized by comprising the following steps: (1) selecting Sichuan and Zhejiang mosaic mustard varieties which have the characteristics of good lump shape, obliquely upward leaf, late bolting, strong cold resistance, harmful bacterium resistance, high yield and high quality; (2) clearing up forest lands; (3) carrying out tillage-free planting; (4) carrying out drought prevention and fertilization; and (5) timely harvesting. In the method disclosed by the invention, the resources such as precious lands and sunshine and the like in established forests are fully used so as to vigorously develop green mustards, which both ensures that the quality of forest trees and the wood production rate are high, and ensures that the quality of mustards, the yield of mustards and the forest farm benefit are high, thereby enriching the social wealth, increasing the earnings of farmers, consolidating the forestation effect, and speeding up the forest and ecological development speed.

The invention belongs to the field of molecular biology, and particularly relates to a molecular marker, a primer pair and a molecular marking method used for identifying the flowering time of brassica oleracea and application of the molecular marker, the primer pair and the molecular marking method. The molecular marker and the primer pair can be used for quick identification of a brassica oleracea breeding material and variety which are resistant to bolting and flower late and for the marker assisted selection of transfer breeding of the material which is resistant to bolting and flowers late, and are of great significance in brassica-oleracea-like vegetable breeding practice and theoretical research on bolting resistance.

The invention relates to a wild harvesting method of Zostera marina L. seeds. The method comprises the following steps: at a low-tide stage when peels on first spathe inflorescences of most flower lateral branches are initially cracked, harvesting flowering branches, putting the harvested flowering branches into 20-mesh bolting silk string bags till each string bag is filled by a half, tightening the mouths of the string bags, connecting the plurality of string bags together, and locally putting the connected string bags back into sea water; 15 days later, taking the string bags out and cleaning substances attached to the surfaces of the string bags; about 30 days later, taking the string bags out, locally pouring the flowering branches in the string bags into a plastic water bucket, adding sea water, stirring vigorously, and picking out residual flowering branches on an upper layer after seeds fall to the bottom of the bucket; adding sea water repeatedly, stirring, and pouring out immature seeds floating on the upper layer; and filtering large shellfishes out by using a 10-mesh mesh sieve in sea water, filtering small shellfishes and residues out by using a 30-mesh mesh sieve, and selecting plump Zostera marina L. seeds. By using the wild harvesting method, a high seed harvesting rate is ensured under the condition of low cost, and damages to populations caused by harvesting of the flowering branches are reduced to the maximum extent. The wild harvesting method can be applied to any Zostera marina L. growing sea area.

The invention discloses an asparagus lettuce cultivation method, and relates to the technical field of vegetable planting. The invention especially relates to an asparagus lettuce planting method. With the method provided by the invention, soil nutrition is enhanced, and an antibacterial effect is provided. The method is suitable for asparagus lettuce growth. The method is characterized in comprising the following steps: 1, an improved variety is selected, wherein a late-maturing spring asparagus lettuce variety that can hardly bolt is selected; 2, a sowing time is determined, wherein greenhouse seedling raising of spring asparagus lettuce is carried out from December to March, and open field seedling raising is carried out from mid-February to late March; 3, spring asparagus lettuce field planting is carried out, wherein field planting is usually carried out in mid-December and preferably on sunny days while the soil is moist, such that seedling root damage can be reduced; 4, fertilizer and water management is reinforced, wherein an overall principle for fertilization is that a base fertilizer should be heavily applied and top dressing should be lightly and frequently applied; and 5, an asparagus lettuce harvesting standard is that an appropriate harvesting time is that when heart leaves are aligned with outer leaves or before squaring. If harvesting is carried out too early, yield is influenced. If harvesting is carried out too late, stem nutrients are consumed, and bolting, flowering, hollowing and stem bark thickening phenomena appear, such that quality is influenced.

The invention provides an astragalus sinicus LHY gene and application thereof. The LHY gene of astragalus sinicus and a sequence of a protein encoded by the LHY gene are shown in SEQ ID NO: 1 and 2. The LHY gene is cloned from the astragalus sinicus for the first time, and the LHY gene is verified to have a function of regulating and controlling plant flowering, in particular, delaying plant flowering, in arabidopsis thaliana through an agrobacterium-mediated genetic transformation method. The LHY transgenic arabidopsis thaliana shows early bolting and early flowering, bolting days are 18.36 days later than wild type, and flowering days are 20.72 days later than wild type. This indicates that LHY gene of astragalus sinicus is closely related to flowering, and has a function of regulating flowering time. The gene is applied to plant property improvement, and has good application prospect. The LHY gene and the encoded protein thereof provide valuable resources for cultivating new plant varieties.

The invention relates to the technical field of oilseed rape planting, in particular to a selenium-rich oilseed rape planting method. The selenium-rich oilseed rape planting method comprises the steps that soil preparation is carried out and a base fertilizer is applied; rape seeds are soaked; the treated rape seeds are sown, and after sowing, the rape seeds are covered with nutrient soil which is prepared by fermenting astragalus sinicus and humic acid and compounding the fermented rape seeds with plant ash; topdressing is carried out, specifically, urea and monopotassium phosphate are topdressed in a 3-4 leaf stage of rape seedlings; a selenium-rich foliar fertilizer is uniformly sprayed once in the early stage of the bud and bolting stage of the oilseed rape, and the selenium-rich foliar fertilizer is prepared from the following raw material components in parts by weight: 2-4 parts of sodium selenite, 8-12 parts of chitosan, 6-8 parts of citric acid, 2-4 parts of r-aminobutyric acid, 3-5 parts of L-calcium aspartate, 12-18 parts of potassium chloride, 3-5 parts of erythritol and 200-300 parts of water; selenium-enriched foliar fertilizer is sprayed once in the flowering stage and the initial stage of seed setting correspondingly; and harvesting is carried out. According to the selenium-rich oilseed rape planting method, oilseed rape growth can be promoted, absorption of oilseed rape to selenium can be improved, and therefore, the selenium content of rapeseeds is increased; and meanwhile, the lodging resistance of the oilseed rape can be enhanced, and the yield of the rapeseeds is increased.

The invention discloses DNA (deoxyribonucleic acid) molecular markers for identifying early bolting of angelica sinensis and application of the DNA molecular markers. According to the invention, the difference of early bolting angelica sinensis and normal angelica sinensis in genome level is studied through an amplified fragment length polymorphic analysis technology, specific molecular marker fragments of the early bolting angelica sinensis are obtained, the sequences of differential fragments are analyzed through a PCR (Polymerase Chain Reaction) technology, and three DNA molecular markers are obtained. According to the invention, AFLP (Amplified Fragment Length Polymorphism) is adopted to screen the molecular markers of the angelica sinensis related to the early bolting, establishment and optimization are performed through a large number of experiments, an AFLP reaction and silver staining reaction system applicable to angelica sinensis seeds is obtained, a clear DNA fingerprint is obtained, and molecular linkage markers of the early bolting character of the angelica sinensis are obtained by screening. Through the invention, the obtained DNA molecular markers by optimization are good in stability and repeatability, can be applied to germplasm identification of the early bolting angelica sinensis, and has significance for breeding an angelica sinensis variety which can resist early bolting.

The invention discloses a rapid allium fistulosum bolting evaluation and classification method which comprises the following steps: 1, preserving allium fistulosum material seeds to be identified in arefrigerator; 2, selecting mature and full seeds, and performing seeding; 3, performing transplanting, namely after seeding, transplanting allium fistulosum seedlings with 3 true leaves into a cultivation tank; 4, putting the cultivation tank into a manual climatic box, and performing low-temperature vernalization; 5, performing bolting and flowering management; and 6, investigating bolting indexes, and performing bolting evaluation. The method has the advantages that a conventional allium fistulosum bolting identification and evaluation cycle is shortened, and the allium fistulosum bolting breeding progress can be accelerated. Limits of natural environments are avoided, the operation feasibility is high, bolting identification and evaluating grading is clear, and the accuracy rate is high.

The invention provides a compound preparation for preventing and treating diseases and insect pests of angelica sinensis and reducing bolting rate of angelica sinensis as well as a preparation methodand application of the compound preparation, and belongs to the technical field of agricultural plant protection, the compound preparation comprises is Isabion, a first component and a second component, wherein the first component is prepared from thiabendazole, fludioxonil and metalaxyl-M; the second component is prepared from cyantraniliprole and thiamethoxam. The compound preparation can prevent and treat diseases and insect pests of angelica sinensis; in addition, the photosynthetic rate and photosynthesis intensity can be improved, the life vitality of angelica sinensis is improved, the resistance of angelica sinensis to high-temperature and drought environments is improved, the growth and development of angelica sinensis roots are promoted, better nutrient absorption is facilitated,the nutrient absorption capacity is enhanced, angelica sinensis roots are luxuriant in leaf reproduction, the bolting rate is reduced, and finally the purposes of fertilizer and pesticide reduction, yield and income increase and the like are achieved. Moreover, the compound preparation is low in cost, simple to operate, capable of achieving reduction and synergy of chemical fertilizers and pesticides and increasing both production and income, and suitable for large-area popularization.

The invention provides a method for breeding bolting green-stem vegetables, and relates to the technical field of breeding new varieties. According to the method described in the invention, an early-bolting high-quality green-stem vegetable material is screened out from a large number of materials, and is hybridized with an early-maturing cabbage heart material with good disease resistance to breed an early-maturing high-quality bolting green-stem vegetable variety required by the market. The characteristics of flowering without vernalization of the cabbage heart material is used to perform hybridizing with the green-stem vegetable easy-bolting parent material, the easy-bolting characteristic of the early-maturing cabbage heart material is transferred to the green-stem vegetable, and an early-maturing, heat-resistant, high-quality and disease-resistant bolting green-stem vegetable new variety which can perform bolting in summer and autumn is bred. The method of the invention can enrichtypes of green-stem vegetables, and breeds early-maturing high-quality bolting green-stem vegetable new varieties to meet market demands.