49 results about "Bolting" patented technology

The invention provides a sunlight greenhouse winter seedling raising method for angelica sinensis. The Chinese angelica sunlight greenhouse winter seedling raising method comprises the steps that a sunlight greenhouse is built in an area with the altitude being 2000 m or below, the azimuth angle of the sunlight greenhouse is 5-7 degrees from the south to the west, the base angle of a front roof is 60-65 degrees, the elevation angle of a main lighting surface is 22-25 degrees, and the elevation angle of a back slope is 37-38 degrees; the method comprises the following steps: sowing angelica sinensis seeds in a greenhouse from late November to early and middle December, wherein the seeds comprise early bolting seeds, side ear seeds or main ear seeds; the environment in the greenhouse is controlled, the temperature in the coldest month is not lower than 12 DEG C, the ground temperature is not lower than 10 DEG C, and vernalization of the angelica sinensis seedlings is avoided; managing the seedlings, including water management, sunlight management, temperature regulation and control, weeding and thinning, and disease and pest control. The Chinese angelica sunlight greenhouse winter seedling raising method provided by the invention has the advantages that Chinese angelica seedling raising is carried out in a zone with the altitude of 2000m or below, and the problem that the ecological environment is damaged by Chinese angelica early bolting, Chinese angelica seed reserving, Chinese angelica prepared land seedling raising and Chinese angelica high-altitude seedling raising can be solved.

The invention discloses a rape waterlogging-resistant gene BnA07ERF151 and an application thereof. The nucleotide sequence of the gene BnA07ERF151 is as shown in SEQ ID NO. 1. The invention also discloses a method for preparing the rape waterlogging-resistant gene BnA07ERF151. According to the invention, a brand new stain-resistant gene BnA07ERF151 is cloned from rape for the first time, and is transferred into arabidopsis thaliana to construct a pure line plant and complete stain resistance identification. Experimental results show that the growth state of a transgenic BnA07ERF151 gene strain in a waterlogging environment is remarkably superior to that of a wild type, bolting development is normal, the survival rate is increased to 43.75% from 12.5%, and the key effect of the gene on enhancing the waterlogging resistance of plants is clearly confirmed; successful cloning of the waterlogging-resistant gene provides a core gene resource for cultivation of new crop varieties, a variety of crops can be endowed with stronger waterlogging tolerance through a transgenic assisted breeding means, and the yield stability under extreme climate is effectively guaranteed, which has great strategic significance in improvement of global agricultural disaster resistance and guarantee of grain safety, and has a wide application prospect. And the method has remarkable social and economic benefits and industrial application prospects.

This invention relates to the field of molecular genetics, specifically to a SNP molecular marker closely linked to a major QTL related to bolting in spinach and its application. The SNP molecular marker provided by this invention is KMBL29, with its polymorphic site located at position 47693313 on spinach chromosome 1, exhibiting an A / G polymorphism. A genotype of AA corresponds to bolting resistance in spinach; genotypes of AG or GG correspond to bolting susceptibility. This invention also provides specific primers based on KASP technology for genotyping this SNP molecular marker. This molecular marker and primers can be used to breed high-quality spinach varieties resistant to bolting, shortening the breeding cycle.

The invention discloses a Chinese angelica out-of-season single seedling raising method suitable for mechanical transplanting and application thereof, and belongs to the field of Chinese herbal medicine seedling raising and cultivation. According to the method, a sunlight greenhouse or a gutter-connected greenhouse with the altitude being 2300 m or above and the lowest temperature being larger than or equal to 8 DEG C in winter is selected, field soil without continuous cropping obstacles is matched with fried rapeseeds, trichoderma harzianum and bacillus subtilis to prepare a special matrix, a seedling raising paper book with the single-hole diameter being 19-25 mm and the height being 12 cm is adopted, pelletized seeds are dibbled in a double-grain mode from late November to early December, and the seeds are sown in the seedling raising paper book with the single-hole diameter being 19-25 mm and the height being 12 cm. Through management of precise temperature and humidity regulation and control, foliage dressing, seedling hardening and leaf cutting and the like, standardized single seedlings are cultivated and transplanted through a transplanter. According to the method, the early bolting rate of the angelica sinensis is reduced, the seedling uniformity and the transplanting survival rate are improved, the transplanting efficiency is improved, the labor cost is reduced, the yield of the angelica sinensis can be increased, efficient utilization of out-of-season resources is achieved, and support is provided for industrial large-scale standardized development.

The invention belongs to the technical field of molecular genetic breeding, and discloses an SNP molecular marker in a Chinese cabbage bolting-resistant gene BrBL1 as well as a KASP primer group and application of the SNP molecular marker. According to the SNP molecular marker, a novel SNP molecular marker of a bolting-resistant gene BrBL1 is positioned through a BSA-seq (bulk nucleic acid-seq) method, the SNP molecular marker is positioned at the 5544261 site on an A02 chromosome of a Chinese cabbage reference genome Brapa-Chiifu-V1.5, and an allelic variation basic group of the SNP site is G / C. The SNP molecular marker has the advantages that the SNP molecular marker can be used for identifying the bolting-resistant gene BrBL1, the bolting-resistant gene BrBL1 can be used for identifying the bolting-resistant gene BrBL1, and the bolting-resistant gene BrBL1 can be used for identifying the bolting-resistant gene BrBL1; the invention further provides a KASP primer group for amplifying the SNP molecular marker, and the primer group consists of nucleotide sequences as shown in SEQ ID NO.3 to SEQ ID NO.5. The invention further provides a KASP primer group for amplifying the SNP molecular marker. The KASP primer group disclosed by the invention can be used for auxiliary selection of the Chinese cabbage bolting-resistant gene BrBL1. The invention further discloses a process for detecting the marker by using a KASP technology, individuals containing the bolting-resistant gene BrBL1 in a Chinese cabbage offspring population can be rapidly screened on the molecular level by using the molecular marker, the accuracy is high, and the breeding efficiency of bolting-resistant varieties is greatly improved.

The application discloses a kind of whole growth period active cold resistance efficient cultivation methods of cabbage seedling, it is related to agricultural planting technical field, the specific steps of the method are as follows: selecting the growth of healthy cabbage seed mother plant, sprays 5-azacytidine and abscisic acid mixed solution, after seed ripening, air-drying is preserved;Seed disinfection is planted in seedling substrate hole tray, conventional management is until 2 leaf 1 heart period;The root group of seedling is placed in low temperature and suitable temperature two independent culture rooms and is carried out partition domestication;After domestication is completed, planting is planted in field, and water and fertilizer management and the prevention and cure of plant disease and insect pest until harvest;The application is improved low-temperature tolerance characteristics and stable genetic offspring by directional cold resistance induction of cabbage bolting period and seed standard treatment from germplasm source head;Root system partition difference temperature domestication is used, avoid root hair root tip damage, maintain normal water and fertilizer absorption function;And supporting link domestication, planting, whole cycle cultivation management system of management, significantly improve planting survival rate.

The application discloses application of a GhELF3 gene in regulating flowering time of plants. The application provides application of any one of the following 1)-3) in regulating flowering time, bolting time and / or rosette leaf number of plants; 1) protein GhELF3; 2) a nucleic acid molecule coding protein GhELF3; and 3) a recombinant carrier, an expression cassette or a recombinant bacterium containing the nucleic acid molecule coding protein GhELF3. A cotton biological clock gene GhELF3 is cloned from Gossypium hirsutum, and the GhELF3 is an orthologous gene of Arabidopsis thaliana AtELF3. The GhELF3 shows an oscillation expression mode within one day, and an expression amount of the GhELF3 in a late-flowering cotton variety is significantly higher than that in an early-flowering cotton variety. An overexpression vector of the gene is constructed, and Arabidopsis thaliana is transformed by a dipping method. It is found that the flowering time of the transgenic Arabidopsis thaliana overexpressing the GhELF3 is obviously later than that of the wild-type Arabidopsis thaliana.

The invention discloses a capsicum annuum cell cycle gene CaTAM and application thereof. The DNA sequence of the capsicum annuum cell cycle gene CaTAM is as shown in SEQ ID No.1. The gene is transformed into Columbia type arabidopsis thaliana through an agrobacterium flower immersion transformation method, a CaTAM heterologous expression arabidopsis thaliana strain is obtained, and it can be known that the heterologous expression of CaTAM can cause early bolting time of arabidopsis thaliana, increase of the number of leaves, decrease of the length and width of the leaves and increase of the root length. The expression of CaTAM of capsicum is silenced through a VIGS technology, a plant with reduced CaTAM expression is obtained, and it can be known that due to silencing of CaTAM, part of pollen cells exit meiosis in advance, secondary meiosis is not carried out, and large pollen grains are formed. It shows that CaTAM plays an important regulation and control role in meiosis, bolting time regulation and control, leaf growth, primary root development and the like, and the gene can be applied to breeding of solanaceae vegetables and other horticultural plants and has a good application prospect.

The invention provides a breeding method of a triploid flowering Chinese cabbage variety, which comprises the following steps of: (1) hybridizing cabbage type rape serving as a female parent and flowering Chinese cabbage serving as a male parent to obtain a triploid hybrid F1 generation; (2) performing phenotype identification on the triploid hybrid F1 generation, and screening combinations which are early in bolting, synchronous in main stem and lateral branch development, obvious in subgenome heterosis and resistant to clubroot; (3) performing a multi-point planting test on the excellent triploid hybrid F1 to verify the yield and disease resistance of the flowering Chinese cabbage; and (4) breeding a special triploid flowering Chinese cabbage variety for seed production. The triploid flowering Chinese cabbage cultivated by the method has the following outstanding advantages of high yield, high economic value, strong disease resistance, multi-round picking and long market supply period. The method provided by the invention solves the problems of lack of special varieties of traditional brassica campestris and low yield of brassica campestris of a dual-purpose variety of brassica campestris, provides a new technical route for germplasm innovation of brassicaceous vegetables, and has important popularization and application values.

The invention provides a mango flowering regulation gene MiABI5-like7 and application thereof, and belongs to the technical field of gene engineering. The invention discovers a new application of the MiABI5-like7 gene in regulating and controlling the flowering of plants, and discovers that the bolting and the flowering period of arabidopsis thaliana are obviously advanced and the quantity of rosette leaves is obviously reduced by heterologous overexpression of the MiABI5-like7 gene in arabidopsis thaliana, which indicates that the gene has the function of promoting the flowering of arabidopsis thaliana. Through silent expression of the MiABI5-like7 gene in mango buds, it is found that the conversion time of the mango flower buds is obviously delayed, the green tip rate (initial flower bud rate) is obviously reduced, it is indicated that silencing of the gene has the function of delaying formation of the mango flower buds, and therefore the MiABI5-like7 gene can also be used for regulating and controlling the flowering time of mangoes.

This invention provides Angelica dahurica AdSVP This invention relates to genes and their applications, belonging to the field of plant molecular biology technology. The Angelica dahurica provided by this invention... AdSVP The CDS sequence of the gene is shown in SEQ ID NO.16, and the amino acid sequence is shown in SEQ ID NO.17. The gene was obtained by overexpression in Arabidopsis thaliana. AdSVP Gene, results showed overexpression AdSVP Arabidopsis plants not only showed significantly greater rates of bolting and rosette leaf number compared to wild-type plants, but also exhibited significantly greater pod length and plant height. AdSVP Gene overexpression not only significantly inhibits flowering in plants and increases the number of rosette leaves in transgenic plants, but also... AdSVP It can also cause transgenic plants to have longer pods and taller plants.

This invention discloses a method for industrialized seedling cultivation of Angelica sinensis and a three-dimensional circulating seedling cultivation device, belonging to the field of traditional Chinese medicinal herb seedling technology. It solves the problems of high reliance on manual labor, poor seedling quality, numerous soil-borne diseases, and low seedling efficiency in traditional Angelica sinensis seedling cultivation. The method includes: non-destructive mechanical dewing, cleaning, grading, pelleting, and quality testing of Angelica sinensis seeds; using peat moss, vermiculite, and perlite as base materials, compounded in a 9:3:1 mass ratio to form a seedling substrate; filling the seedling substrate into Angelica sinensis-specific seedling trays; sowing the pelleted seeds; covering with soil and watering; placing the seedling trays in a three-dimensional circulating Angelica sinensis seedling cultivation device for precise environmental control to cultivate seedlings; transferring the seedlings to a cold storage for low-temperature dormancy storage; and transplanting after vernalization. This invention achieves mechanization, intelligentization, and intensification of Angelica sinensis seedling cultivation, reducing the total cost of seedling cultivation and the seedling bolting rate, improving the transplant survival rate, saving land, and is suitable for large-scale industrial application.

The invention discloses a rape flower sea landscape modeling method based on unmanned aerial vehicle suspender cutting and topping and a matched device, and the method comprises the steps: carrying out the selective cutting and topping operation of a preset pattern region of a rape field in a bolting period through an unmanned aerial vehicle suspender cutting and topping device by utilizing the characteristic that rape crops can recover to grow within a period of time after bolting is removed; the flowering time difference of the oilseed rapes in the cutting area and the non-cutting area is realized, so that the landscape visual effect of twice flowering periods is formed. The growth process of the rape in the flowering period in the pattern area is reversely regulated and controlled through topping, the flower sea landscape modeling effect under the single-variety planting condition of the rape crops is rapidly achieved within a short period, and the viewing duration is prolonged by about two weeks. Compared with a traditional multi-variety mixed planting crop landscape modeling method, selective treatment only needs to be conducted in the bud and bolting period of conventional rape planting, operation is flexible, the device is simple, landscape modeling is high in timeliness and reliability, and special treatment is not needed during later grain harvesting.

The invention provides a brassica napus BnaABI5 gene site-directed mutagenesis method and application, and belongs to the technical field of plant gene editing and plant breeding. According to the invention, the application of the brassica napus BnaABI5 gene in negative regulation of low-temperature growth of plants is found, and the brassica napus BnaABI5 gene is subjected to site-directed mutagenesis by using a CIRSPR / Cas9 system for breeding; according to the invention, a BnaABI5 function deletion mutant is constructed by utilizing a CRISPR-Cas9 gene editing technology, so that a new rape germplasm adapting to a low-temperature growth condition and early bolting is created, and the practicability is very good.

The invention relates to an application of a GhSVP gene and a protein thereof in regulation and control of cotton flowering, and discloses a method for changing the cotton flowering time by regulating and controlling the expression of the GhSVP gene and an application of the method in early-maturing cotton breeding. After heterologous expression of the GhSVP gene in arabidopsis thaliana, bolting and flowering of a transgenic line are delayed, the number of rosette leaves is increased during flowering, and vegetative growth of arabidopsis thaliana is prolonged; after the GhSVP gene is silenced in the cotton, the cotton budding and flowering time is advanced, which indicates that the gene can regulate and control the flowering time of the cotton. As a potential cotton flowering regulation gene, the GhSVP has important significance for improving the early maturing character of cotton to meet the requirements of growth cycles of cotton in different ecological regions.

The invention belongs to the technical field of agricultural breeding equipment, and particularly relates to a comparison experiment device for rape seed selection and cultivation and a comparison method thereof.The comparison experiment device comprises a base, a motor is fixed to the upper surface of the base, a vertical rod is fixedly connected to the driving end of the motor in a vertically upward mode, and a cross plate is fixed to the upper end of the vertical rod; a sliding seat sleeves the end part of the cross plate in a sliding manner; the comparison method comprises the following steps of planting, spacing adjustment, soil pressure adjustment and transpiration rate calculation. The vertical rod is driven by the motor to drive the cross plate to rotate, the sliding seat is pulled to move in the radial direction in cooperation with the rotating disc controlled by the motor and the connecting rod mechanism, and dynamic distance adjustment of the rape breeding tank in the seedling stage, the bolting stage and the full-bloom stage is achieved; the mechanical resistance of soil from loosening to compacting is simulated by pressurizing the annular air bag layer in stages, and the transpiration rate is calculated by monitoring the weight change of plants in real time by combining a weighing sensor.

This invention relates to a cultivation method for increasing the alliin content of garlic and its application, belonging to the field of vegetable cultivation technology. The invention provides a cultivation method for increasing the alliin content of garlic, comprising the following steps: (1) applying potassium sulfate aqueous solution during the garlic seedling stage; (2) applying potassium magnesium sulfate aqueous solution and cysteine ​​aqueous solution during the garlic growth stage; (3) manually removing all flower stalks during the garlic bolting stage, and simultaneously spraying paclobutrazol aqueous solution on the leaves; (4) applying potassium magnesium sulfate aqueous solution and valine aqueous solution during the garlic bulb enlargement stage; (5) spraying cold water onto the leaves before harvesting the garlic bulbs until water droplets remain on the leaves. The cultivation method of this invention is simple to operate, easy to learn and master, and has low input costs. Using this cultivation method to grow garlic can significantly increase the alliin content in garlic, resulting in garlic with an alliin content of 1.24% to 1.34%.

The application discloses an LsBOR5b gene and application thereof in regulating and marking a plant bolting time, relates to the field of biotechnology, and prolongs the bolting and flowering time of the lettuce and improves the yield by overexpressing the expression of the LsBOR5b homologous gene in the plant, has important theoretical research value and wide practical application prospect for cultivating the lettuce plant with long bolting and flowering time. Meanwhile, the biological sensor can be developed based on the expression specificity of the specific gene in the lettuce in the specific tissue and development stage disclosed by the present application; for example, the biological sensor for detecting the bolting signal is constructed based on the gene LsBOR5b with obvious expression change in the initial bolting stage, the sensor sends a signal when the expression amount of the gene reaches a certain threshold value, the lettuce is about to bolt, and the harvesting, adjustment of the environmental or nutrient supply conditions, collection of test data or other corresponding measures are carried out as required.

The application provides a breeding method of a bolting-resistant carrot inbred line, which comprises the following steps: sowing carrot seed materials in a southern region where carrots cannot complete vernalization in winter in summer and autumn, selecting non-bolting seed roots from the carrot breeding materials in mid-March, transplanting the carrot breeding materials to a northern region, selfing when the carrot breeding materials blossom, and repeating the above steps for three times to obtain the bolting-resistant carrot inbred line. The method can be applied to carrot breeding, directly selects the bolting-resistant carrot inbred line, provides a parent for breeding a new bolting-resistant carrot variety, and provides a reference for breeding other plants in the selection of a bolting-resistant inbred line. The method is simple and easy to operate, and can accelerate the breeding of the bolting-resistant carrot inbred line. The bolting-resistant carrot inbred line used for carrot breeding has the advantages of low cost, good effect, high repeatability and simple operation.

The invention discloses a seedling raising method for preventing early bolting of angelica sinensis in a greenhouse environment. The invention belongs to the technical field of plant cultivation. Through an integrated environment precise control system, a biological regulation and control strategy and a post-processing technology, the inherent defects that an existing angelica sinensis seedling raising technology passively depends on a specific high-altitude natural environment, the bolting rate is high, the growth cycle is long, and labor input is huge are overcome fundamentally; according to the method, active, accurate and controllable management is carried out on key environmental factors (temperature and illumination) for starting reproduction and growth of angelica sinensis, and physiological vernalization blocking measures are introduced in a specific stage, so that the early bolting phenomenon of angelica sinensis is effectively inhibited, the seedling raising period is remarkably shortened, the suitable planting area is enlarged, and the yield of seedlings is increased. And the labor investment in the production process is greatly reduced.

The application discloses a sapindus mume SmSOC2 gene and application thereof, the gene is expressed in plants to regulate flowering time, equal height of pistil and stamen, and stamen loss and variation, the gene is a nucleotide sequence shown in a sequence table Seq ID NO.1 or a nucleotide sequence encoding an amino acid sequence shown in a sequence table Seq ID NO.2.The gene is used for cultivating sapindus mume new varieties with different flowering times.Through whole plant observation and flowering time difference analysis of transgenic arabidopsis, overexpression of the gene can make the plant early in the bolting and flowering time, equal height of pistil and stamen, and stamen loss and variation.The flowering time, bolting days and rosette leaf number of the transgenic arabidopsis plant are significantly different from those of the wild type plant, which is helpful to accurately improve the flower structure of the plant, cultivate new varieties with higher ornamental value, speed up the sapindus mume breeding cycle and cultivate high-yield varieties.

The invention discloses a peucedanum praeruptorum intercropping and crop rotation intensive planting method, and relates to the technical field of peucedanum praeruptorum planting, and the method comprises the following steps: S1) preparation before planting: land selection and soil preparation: selecting a gentle slope land with a deep soil layer and loose soil texture, deeply plowing in winter, airing for about one month, raking the land before sowing, and digging compartments; seed soaking and dressing: common hogfenneI roots are sown in February to March, hogfenneI roots seeds are soaked in water at normal temperature one day before sowing, the seeds are fished out, the surfaces of the seeds are aired, and the seeds and plant ash are uniformly mixed for standby application; according to the peucedanum praeruptorum dunn growth characteristics and the planting period, proper crops are screened for intercropping and crop rotation with peucedanum praeruptorum dunn, on one hand, bolting of peucedanum praeruptorum dunn is reduced, the yield and quality of medicinal materials are improved, on the other hand, crop planting is increased, the grain yield and income are improved, and excessive non-grain cultivation of cultivated land is prevented through the intercropping and crop rotation method; the seed germination rate is improved and the planting cost is reduced.

The invention belongs to the technical field of genetic breeding of rape, and particularly discloses an InDel molecular marker related to anthocyanin accumulation of a flowering stalk in a brassica napus bolting period and application of the InDel molecular marker. The InDel molecular marker provided by the invention is closely linked with the enrichment of anthocyanin in brassica napus flower stems, and the marker is used for detecting early seedlings of brassica napus, so that whether the flower stems of brassica napus varieties have anthocyanin accumulation in a bolting period or not can be accurately and efficiently identified in an early stage; the variety of the purple-bolted brassica napus with the flower stems rich in anthocyanin under the natural condition can be identified under the condition that illumination is weak and anthocyanin accumulation is not facilitated, and rapid breeding of the purple-bolted brassica napus with the flower stems having anthocyanin accumulation can be carried out in a greenhouse. Compared with an identification technology of other marker types such as SNP, a reagent developed based on the InDel molecular marker can complete detection by utilizing common PCR and agarose gel electrophoresis, sequencing is not needed, the operation is simple, convenient and rapid, and the cost is low.

The application discloses a sapindus mume SmSOC3 gene and application thereof, the gene is expressed in plants to regulate flowering time, pistil and stamen length and calyx shape, the gene is a nucleotide sequence shown in a sequence table Seq ID NO.1 or a nucleotide sequence encoding an amino acid sequence shown in a sequence table Seq ID NO.2.The gene is used for cultivating sapindus mume new varieties with different flowering time and different flower types.Through whole plant observation and flowering time difference analysis of transgenic arabidopsis, overexpression of the gene can make the plant bolting and flowering time in advance, the development height of pistil and stamen is equal but both slightly short, and the calyx shape is changed into conical shape.The flowering time, bolting days and rosette leaf number of the transgenic arabidopsis plant are significantly different from those of the wild type plant, which is beneficial to accelerating sapindus mume breeding period, cultivating ornamental varieties with different flower types and accelerating cultivation of new varieties with high yield.

The application discloses a m6A demethylase-regulated radish bolting and flowering gene RsSUF4 and application thereof, relates to the field of bioengineering technology, and has the technical points that the gene is RsSUF4. The functions of the demethylase RsALKBH10B and the inhibited flowering gene RsSUF4 gene in the flowering process are analyzed, and the specific correlation mechanism between m6A modification and radish flowering regulation is determined.

The invention belongs to the technical field of cultivation and breeding, and particularly relates to a method for regulating vernalization of celery and inducing bolting and flowering, which comprises the following steps: selecting celery seedlings with 4-6 true leaves, and performing vernalization treatment to obtain celery plants; the vernalization treatment is light-dark alternate culture; the duration and temperature conditions are limited; after vernalization treatment, performing bolting and flowering induction on the celery plants; the bolting and flowering induction is light-dark alternate culture; and the duration and temperature conditions are limited. According to the method, the temperature and sunshine duration parameters are accurately regulated and controlled, so that the artificial controllable induction of bolting and flowering processes of different celery varieties is realized, the variety adaptation limitation in the prior art is thoroughly solved, the breeding period of the celery varieties is effectively shortened, and technical support is provided for rapid breeding of good celery varieties.

The present invention relates to a Lactuca sativa plant having delayed bolting and / or shade tolerance, wherein said plant comprises in its genome at least one copy of a mutant allele of the Phytochrome B (LsPhyB) gene. The present invention provides a seed from which the Lactuca sativa plant according to the present invention can be grown.

The application discloses a Chinese cabbage bolting and flowering resistance related gene BrAHL24, BrGLK2 and application thereof, and belongs to the technical field of biotechnology.The Chinese cabbage bolting and flowering resistance related gene is a Chinese cabbage BrAHL24a gene, a Chinese cabbage BrAHL24b gene, a Chinese cabbage BrGLK2a gene or a Chinese cabbage BrGLK2b gene.The application clones the Chinese cabbage BrAHL24 gene or the Chinese cabbage BrGLK2 gene, and identifies the function of the gene by using a transgenic mode, finds the effect of the gene on controlling bolting and flowering time of cruciferous plants, and finds that overexpression of the Chinese cabbage BrAHL24a gene, the Chinese cabbage BrAHL24b gene, the Chinese cabbage BrGLK2a gene or the Chinese cabbage BrGLK2b gene can significantly delay bolting and flowering of cruciferous plants such as Arabidopsis thaliana and Chinese cabbage, and has a good application prospect in genetic engineering breeding.

The application discloses a method for identifying the petal color of Chinese cabbage in the seedling stage. The method uses the DNA of Chinese cabbage varieties as a template, adopts a primer group wF35 to amplify the fragment through polymerase chain reaction (PCR), and according to the size of the target band after electrophoresis, the petal color of Chinese cabbage varieties can be preliminarily inferred before bolting and flowering, and corresponding petal color varieties are screened. The method provided by the application can quickly detect the petal color of Chinese cabbage varieties, realize early and accurate prediction of the petal color, greatly save time and resources, greatly improve the selection efficiency, thereby accelerate the breeding process, and has an important role in production practice.

The application relates to the technical field of plant genetic engineering, and particularly discloses LsMAPK6 Protein and its coding gene and application thereof in regulating high-temperature bolting performance of leaf lettuce LsMAPK6 The application finds that the high-temperature bolting performance of leaf lettuce can be regulated by controlling the expression of the protein. LsMAPK6 The application discloses the protein or the coding gene thereof or biological material containing the coding gene and application thereof in regulating the high-temperature bolting performance of leaf lettuce; the high-temperature bolting performance of the leaf lettuce refers to the bolting performance of the leaf lettuce after being treated at 33±2 DEG C. The application has great significance for exploring the high-temperature bolting mechanism, formulating scientific management measures and creating excellent germplasm resistant to bolting.