24 results about "Spinacia" patented technology

The invention provides an oral thylakoid product for resisting organ failure and aging, which is prepared by the following steps: extracting thylakoid from plants including spinach, arabidopsis thaliana, lettuce, cabbage, green algae or wheat seedlings, redissolving the thylakoid into a buffer salt solution A, and carrying out ultrasonic treatment for 10-20 minutes under the ultrasonic power of 200-500W to prepare a suspension; adjusting the concentration of the suspension to 0.5-2 mg / ml, and obtaining a nano capsule-like body with the particle size of 200-500 nm by using a microfluidic technology; the buffer salt solution A is prepared from 10 to 100 mmol / L of 4-hydroxyethyl piperazine ethanesulfonic acid (HEPES), 1 to 2 mmol / L of ethylenediamine tetraacetic acid (EDTA) disodium, 0.5 to 4 mmol / L of magnesium chloride (MgCl2), 1 to 10 mmol / L of ascorbic acid and 100 to 500 mmol / L of sapoeda sodium (SNAC), and the pH value of the buffer salt solution A is adjusted to 7.5 to 8.0; and the concentration of the capsule in the buffer salt solution A is 100-400 mg / mL. According to the product, self QPRT enzyme of the thylakoid is used for efficiently catalyzing de novo synthesis of NAD + of whole body tissues and organs, so that multiple physiological processes such as energy synthesis enhancement, oxidative stress reduction and inflammatory reaction relief are finally realized, organ failure is efficiently reversed, and organ aging is relieved.

The current invention relates to spinach plants comprising a gene or locus which leads to a broad spectrum resistance to Peronospora effusa (Pe). The invention also relates to progeny of said 5 spinach plants, to propagation material of said spinach plants, to a cell of said spinach plants, to seed of said spinach plants, and to harvested leaves of said spinach plants. The invention also relates to use of said spinach plants in breeding to confer resistance against Peronospora effusa. The invention further relates to a method for generating a spinach plant having a broad spectrum resistance to Peronospora effusa.

The invention relates to the technical field of agricultural biological agents, and particularly provides trichoderma harzianum as well as a compound preparation and application thereof. The trichoderma harzianum has a remarkable inhibition effect on six common plant pathogenic bacteria such as sclerotinia sclerotiorum, botrytis cinerea and fusarium oxysporum. The fermentation liquor has a remarkable growth promoting effect on various plants such as pakchoi, spinach and flowering Chinese cabbage when being diluted by 10-100 times, and the fresh weight, the dry weight and the chlorophyll content of the plants can be increased. The compound liquid is formed by compounding trichoderma harzianum fermentation liquor and a hypecoum erectum alkaloid extract with the concentration of 50 ppm, the effective dilution interval of the trichoderma harzianum fermentation liquor in the compound liquid is 10-100 times, and the compound liquid has an inhibition effect on cowpea fusarium wilt germs, promotes growth of cowpeas and nicotiana benthamiana at the same time, achieves integration of disease prevention and growth promotion and has a good application prospect. Wide agricultural application prospects are realized.

The present invention relates to an allele designated alpha-WOLF 24 which confers resistance to at least one Peronospora farinosa f. sp. spinacea race, wherein the protein encoded by said allele is a CC-NBS-LRR protein that comprises in its amino acid sequence: a) the motif “MAEIGYSVC” (SEQ ID NO: 1) at its N-terminus; and b) the motif “KWMCLR” (SEQ ID NO: 2); and wherein the LRR domain of the protein has in order of increased preference at least 95%, 96%, 97%, 98%, 98.2%, 98.5%, 98.8%, 99%, 100% sequence similarity to SEQ ID NO: 10. The allele when present in a spinach plant confers complete resistance to at least Peronospora farinosa f. sp. spinacea race Pfs: 1, Pfs: 2, Pfs: 5, Pfs: 6, Pfs: 7, Pfs: 9, Pfs: 11, Pfs: 13, Pfs: 15 and Pfs: 17, and does not confer resistance to downy mildew race Pfs: 16.

The invention provides a composition for inhibiting protein carbonylation and application of the composition. The composition of the present application comprises a first component and a second component, the first component comprising an umbelliferae carrots extract, a cruciferae brassica extract, an azaleaceae vaccinium extract, and an amaranaceae spinach extract as active ingredients; the second component comprises tocopheryl acetate as an active ingredient, and the mass ratio of the active ingredient of the first component to the active ingredient of the second component is 1: 20000 to 3.2: 1. By utilizing the composition provided by the invention, cell protein carbonylation caused by oxidative stress can be delayed, so that the aging state of a human body is improved. The main components of the composition are plant extracts, so that the composition is low in skin irritation, safe, effective and environment-friendly, and a new direction is developed for the development of pure natural whitening and anti-aging cosmetics. The invention further provides a preparation method of the composition and application of the composition in preparation of products for inhibiting protein carbonylation.

The application discloses a direct organogenesis genetic transformation method based on spinach stem tips and axillary buds, and relates to the technical field of plant genetic transformation. pCAMBIA1305-GUS As a plant expression vector, the Agrobacterium GV3101 (pSoup-p19) is used as a receptor Agrobacterium to establish a stable genetic transformation system of spinach. The application utilizes the regeneration capacity of the spinach stem tip meristem and axillary bud meristem, can improve the regeneration efficiency and transformation efficiency, and significantly shortens the genetic transformation cycle. The system can be used for the function research of key regulation genes of agronomic traits of spinach and the cultivation of excellent spinach varieties.

The invention provides seeds and plants of spinach hybrid SVVB2646. The invention thus relates to the plants, seeds, plant parts, and tissue cultures of spinach hybrid SVVB2646 and to methods for producing a spinach plant produced by crossing such plants with themselves or with another plant, such as a spinach plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to plants, seeds, plant parts, and tissue cultures of spinach hybrid SVVB2646 comprising introduced beneficial or desirable traits.

This invention relates to the field of molecular genetics, disclosing SNP molecular markers associated with the mottled leaf phenotype of spinach and their applications. The SNP molecular markers contain a nucleotide sequence with a G / A polymorphism at position 158522852 bp on spinach chromosome 3; spinach plants with the G base have normal leaves, while those with the A base exhibit mottled chlorotic leaves. This invention utilizes segregating population analysis (BSA) to discover SNPs associated with the mottled leaf phenotype of spinach and develops a KASP primer combination for detecting the molecular markers for assisted breeding. The three KASP primers provided by this invention can detect the mottled leaf phenotype of spinach at the seedling stage, with high throughput and high accuracy. The co-dominant KASP markers provided by this invention facilitate the screening of spinach varieties containing the mottled leaf gene, significantly accelerating the breeding process of spinach varieties containing the potential mottled leaf gene.

A spinacia plant with a low oxalic acid content, in particular a low oxalic acid content that is stable and unaffected by environmental conditions. A method for producing the same, a screening method, and a screening kit. A spinacia plant with a low oxalic acid content having at least one low oxalic acid locus located in a chromosome 4 region; a method for producing the spinacia plant having the low oxalic acid content through cross breeding the spinacia plant having the low oxalic acid content with another spinacia plant. A method for screening spinacia plants with low oxalic acid contents by selecting the spinacia plant having at least one low oxalic acid locus located in the chromosome 4 region. A kit for screening spinacia plants with low oxalic acid contents having a primer or a probe that is specifically bound to the low oxalic acid locus.

The present invention aims to provide spinach plants that are resistant to downy mildew. [Solution] The present invention provides a spinach plant including a spinach plant or its progeny line identified by accession number FERM BP-22502 or FERM BP-22503. The spinach plant of the present invention exhibits resistance to downy mildew, preferably to one or more selected from downy mildew races Pfs2, Pfs4, Pfs5, Pfs6, Pfs7, Pfs10, Pfs11, Pfs12, Pfs13, Pfs14, Pfs15, Pfs16, Pfs17, Pfs18, and Pfs19.

Provided herein are spinach plants resistant to Peronospora farinosa and Stemphylium vesicarium, and spinach plants additionally resistant to Cucumber Mosaic Virus (CMV). Also provided herein are genomic fragments providing the present resistances and use thereof for identifying spinach resistant to Peronospora farinosa and Stemphylium vesicarium, and spinach plants additionally resistant to Cucumber Mosaic Virus (CMV). Provided herein are a spinach plant resistant to Peronospora farinosa and Stemphylium vesicarium: including a genomic fragment of Spinacia tetrandra located on chromosome 4 between positions 8255074 and 8620598 of the spinach reference genome, the genomic fragment of Spinacia tetrandra lacking a lethal factor and providing Peronospora farinosa resistance and including a genomic fragment located on chromosome 3 and between positions 1177586 and 1271037 of the spinach reference genome providing Stemphylium vesicarium resistance.

The present invention relates to downy mildew, and especially downy mildew caused by the plant pathogen Peronospora farinosa, resistant spinach plants (Spinacia oleracea). The present spinach plants include a downy mildew resistance providing genomic fragment from Spinacia tetrandra. Specifically, the present invention relates to spinach plants being resistant to downy mildew, wherein the spinach plant includes a downy mildew resistance providing genomic fragment from Spinacia tetrandra such as spinach plants including in their genomes one or more nucleic acid sequences selected from the groups consisting of SEQ ID No. 1, SEQ ID No. 3, SEQ ID No. 5, SEQ ID No. 7, SEQ ID No. 9, SEQ ID No. 11, SEQ ID No. 13, SEQ ID No. 15, SEQ ID No. 17, SEQ ID No. 19, SEQ ID No. 21, SEQ ID No. 23, SEQ ID No. 25, SEQ ID No. 27, SEQ ID No. 29, SEQ ID No. 31, SEQ ID No. 33 and SEQ ID No. 35.

The present invention is a downy mildew resistant spinach plant having the downy mildew resistance RTM-1 gene present on chromosome 4 of the tetra spinach line CGN25466:MGK 01; the above recited downy mildew resistant spinach plant wherein the RTM-1 gene is a gene present on chromosome 4 of the tetra spinach line CGN25466:MGK 01 in the range of chr4_7962907 to chr4_8617232; the downy mildew resistant spinach plant of any one recited having resistance to at least race Pfs1, Pfs2, Pfs3, Pfs4, Pfs5, Pfs6, Pfs7, Pfs8, Pfs9, Pfs10, Pfs11, Pfs12, Pfs13, Pfs14, Pfs15, Pfs16, Pfs17, Pfs18, Pfs19, and UA1014 race exhibited by the Pseudoperonospora farinosa f. sp. esculenta strains.

The present application relates to the technical field of agricultural biological agents, and specifically provides a Trichoderma harzianum, a compound preparation thereof and application.The Trichoderma harzianum has a significant inhibitory effect on six common plant pathogenic fungi such as Sclerotinia sclerotiorum, Botrytis cinerea and Fusarium oxysporum.The fermentation liquor thereof has a significant growth-promoting effect on a variety of plants such as Chinese cabbage, spinach and flowering Chinese kale in a dilution range of 10-100 times, and can improve the fresh weight, dry weight and chlorophyll content of the plants.The compound liquid is compounded by the Trichoderma harzianum fermentation liquor and 50 ppm concentration of the extract of angelica polymorpha alkaloids, wherein the effective dilution range of the Trichoderma harzianum fermentation liquor in the compound liquid is 10-100 times, the compound liquid has an inhibitory effect on Fusarium oxysporum, and simultaneously promotes the growth of cowpea and Nicotiana benthamiana, realizes the integration of disease prevention and growth promotion, and has a broad agricultural application prospect.

The invention provides seeds and plants of spinach hybrid SVVB2772. The invention thus relates to the plants, seeds, plant parts, and tissue cultures of spinach hybrid SVVB2772 and to methods for producing a spinach plant produced by crossing such plants with themselves or with another plant, such as a spinach plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to plants, seeds, plant parts, and tissue cultures of spinach hybrid SVVB2772 comprising introduced beneficial or desirable traits.

The present invention provides spinach plants that exhibit resistance to downy mildew caused by downy mildew, as well as methods of producing, identifying, or selecting a plant or germplasm having a downy mildew resistance phenotype. Such plants include spinach plants comprising a genomic introgression region conferring pest resistance. Further provided are compositions comprising novel polymorphic markers for the detection of plants comprising introgression loci.

The present invention relates to a Spinacia oleracea plant comprising a nucleic acid molecule which when homozygously present provides at least intermediate resistance to Peronospora effusa races Pe: 1, Pe: 2, Pe: 3, Pe: 4, Pe: 5, Pe: 6, Pe: 7, Pe: 8, Pe: 9, Pe: 10, Pe: 11, Pe: 12, Pe: 13, Pe: 14, Pe: 15, Pe: 16, Pe: 17, Pe: 18, Pe: 19 and Pe: 20, wherein said nucleic acid molecule comprises a gene which comprises a coding sequence according to SEQ ID NO: 1 and a gene which comprises a coding sequence according to SEQ ID NO: 2, or a gene which comprises a coding sequence having at least 95.0% sequence identity to SEQ ID NO: 7 and comprises an insertion of sequence motif GAAATA between nucleotides 1235 and 1236 of SEQ ID NO: 7 and a gene which comprises a coding sequence having at least 95.0% sequence identity to SEQ ID NO: 8 and lacks a sequence according to SEQ ID NO: 14, wherein the 95.0% sequence identity is over the part of SEQ ID NO: 8 without the lacking sequence, or genes comprising the corresponding genomic sequences or genes encoding a protein having an amino acid sequence according to SEQ ID NO: 3, and a gene encoding a protein having an amino acid sequence according to SEQ ID NO: 4, or genes encoding proteins showing 95% sequence identity thereto. The invention further relates to a plant carrying the nucleic acid molecule and to methods of selecting, producing and growing such plants.

The present invention relates to an allele designated alpha-WOLF 31 which confers resistance to at least one Peronospora effusa race, wherein the protein encoded by said allele is a CC-NBS-LRR protein that comprises in its amino acid sequence: a) the motif "MAEIGYSVC" at its N-terminus; and b) the motif "KWMCLR"; and wherein the protein has an amino acid sequence that in order of increased preference has at least 97% sequence similarity to SEQ ID NO: 11. The allele when present in a spinach plant confers complete resistance to at least Peronospora effusa race Pe:1, Pe:2, Pe:4, Pe:5, Pe:6, Pe:7, Pe:10, Pe:12, Pe:13, Pe:14, Pe:15, Pe:16, Pe:18, Pe:19, and Pe:20.

The present invention relates to a spinach plant comprising a gene or a locus that results in a broad spectrum of resistance to Peronospora diffusa (Pe). The invention also relates to progeny of said spinach plant, propagation material of said spinach plant, cells of said spinach plant, seeds of said spinach plant and harvested leaves of said spinach plant. The invention also relates to the use of said spinach plant to confer resistance against downy mildew in breeding. The present invention also relates to a method of producing a spinach plant having a broad spectrum resistance against Peronospora dispersa (Pe).

The invention discloses a method for extracting ribulose-1, 5-diphosphate carboxylase / oxygenase (RuBisCO) from plant leaves, and belongs to the field of plant protein extraction. Comprising the following steps: (1) mixing plant leaves with water, adding sodium pyrosulfite and cross-linked polyvinylpyrrolidone, pulping, crushing, stirring and centrifuging to obtain a protein crude extract; (2) adjusting the pH value of the protein crude extract, heating, centrifuging, and carrying out suction filtration to obtain a permeate; (3) carrying out ultrafiltration on the permeate to obtain a protein concentrated solution; and (4) drying the protein concentrated solution to obtain RuBisCO protein powder. The plant leaves are at least one of green leaves such as medicago sativa and spinach. The mass ratio of the plant leaves to the water is 1: (1-49); the addition amount of the sodium pyrosulfite is 0.2%-2% of the total mass of the plant leaves and the water; the addition amount of the cross-linked polyvinylpyrrolidone is 0.5%-3% of the total mass of the plant leaves and the water. The extraction method provided by the invention can achieve the effects of high protein content and recovery rate, simple operation process and cost saving.

This invention relates to an autism spectrum disorder modifier, comprising the following raw materials in parts by weight: 20-40 parts of fructooligosaccharides, 20-40 parts of galactooligosaccharides, 10-30 parts of spinach powder, 1-20 parts of Poria cocos powder, and 1-20 parts of blueberry powder. The autism spectrum disorder modifier of this invention is safe and effective. The various components work synergistically through different pathways to reduce the secretion of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α) and the inflammatory mediator NO, inhibit the release of inflammatory mediators and pro-inflammatory factors, and balance inhibitory and excitatory circuits; it also acts on the release and synthesis of neurotransmitters; regulates the intestinal flora, increases the relative abundance of lactic acid and acetate-producing Bifidobacteriaceae, Enterococciceae, and Lactobacilliceae, as well as lactic acid and acetate-utilizing Prevotellaceae and Lachnospiraceae genera, increases the content of short-chain fatty acids, and improves the clinical symptoms of autism spectrum disorder.

The invention provides a red light driven-spinach chlorophyll mediated novel coumarin and tetrahydroisoquinoline coupling method and application, a tetrahydroisoquinoline compound, a coumarin compound, a chlorophyll photosensitizer and a solvent are mixed according to a ratio, and an illumination reaction is performed in an air atmosphere; coupling the skeleton of the tetrahydroisoquinoline compound with the skeleton of the coumarin compound, and purifying to obtain the compound with both the coumarin skeleton and the tetrahydroisoquinoline skeleton. According to the red light driven-spinach chlorophyll mediated novel coumarin and tetrahydroisoquinoline coupling method and application, operation is easy and convenient, environment friendliness is achieved, and efficient coupling is achieved.

The present invention relates to an allele designated beta-WOLF 28 which confers resistance to at least one race of Peronospora farinosa (P. farinosa) Peronospora effusa ​ ) wherein the protein encoded by said allele is a CC-NBS-LRR protein comprising in its amino acid sequence: a) at its N-terminus the motif "MAEIGYSVC"; and b) the motif "HVGCVVDR"; and wherein said protein has an amino acid sequence having in order of increasing preference at least 95.5% sequence similarity with SEQ ID NO: 11. When present in homozygous form in a spinach plant, said allele confers full resistance to at least P. farinosa races Pe:1, Pe:2, Pe:3, Pe:4, Pe:5, Pe:6, Pe:7, Pe:8, Pe:9, Pe:10, Pe:11, Pe:12 and Pe:13 and does not confer resistance to races Pe:14, Pe:15, Pe:16, Pe:17, Pe:18 and Pe:19.