45 results about "Spinacia" patented technology

The invention provides seeds and plants of spinach hybrid SVVB2741. The invention thus relates to the plants, seeds, plant parts, and tissue cultures of spinach hybrid SVVB2741 and to methods for producing a spinach plant produced by crossing such plants with themselves or with another plant, such as a spinach plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to plants, seeds, plant parts, and tissue cultures of spinach hybrid SVVB2741 comprising introduced beneficial or desirable traits.

The present invention relates to a plant composition with whitening and freckle-removing effects and its application, belonging to the technical field of compositions. The plant composition comprises the following raw materials: sea fennel extract, osmanthus extract, barley extract, smooth fruit elm bark extract, black sesame extract, thyme extract, spinach extract and a solvent. The present invention combines sea fennel extract, osmanthus extract and barley extract, starting from a triple whitening mechanism of inhibiting tyrosinase activity, inhibiting melanin formation caused by α-MSH, and inhibiting melanocyte activity, to synergistically improve the whitening effect of the composition. In addition, three freckle-removing plant extracts are added to the formula of the present invention. The smooth fruit elm bark extract is used to reduce freckles, the black sesame extract is used to enhance the cell melanin metabolism ability, and the thyme extract is used to stimulate the growth of new cells. The three make the composition prepared by the present invention achieve a synergistic effect of freckle-removing from three perspectives.

The invention provides an oral thylakoid product for resisting organ failure and aging, which is prepared by the following steps: extracting thylakoid from plants including spinach, arabidopsis thaliana, lettuce, cabbage, green algae or wheat seedlings, redissolving the thylakoid into a buffer salt solution A, and carrying out ultrasonic treatment for 10-20 minutes under the ultrasonic power of 200-500W to prepare a suspension; adjusting the concentration of the suspension to 0.5-2 mg / ml, and obtaining a nano capsule-like body with the particle size of 200-500 nm by using a microfluidic technology; the buffer salt solution A is prepared from 10 to 100 mmol / L of 4-hydroxyethyl piperazine ethanesulfonic acid (HEPES), 1 to 2 mmol / L of ethylenediamine tetraacetic acid (EDTA) disodium, 0.5 to 4 mmol / L of magnesium chloride (MgCl2), 1 to 10 mmol / L of ascorbic acid and 100 to 500 mmol / L of sapoeda sodium (SNAC), and the pH value of the buffer salt solution A is adjusted to 7.5 to 8.0; and the concentration of the capsule in the buffer salt solution A is 100-400 mg / mL. According to the product, self QPRT enzyme of the thylakoid is used for efficiently catalyzing de novo synthesis of NAD + of whole body tissues and organs, so that multiple physiological processes such as energy synthesis enhancement, oxidative stress reduction and inflammatory reaction relief are finally realized, organ failure is efficiently reversed, and organ aging is relieved.

The current invention relates to spinach plants comprising a gene or locus which leads to a broad spectrum resistance to Peronospora effusa (Pe). The invention also relates to progeny of said 5 spinach plants, to propagation material of said spinach plants, to a cell of said spinach plants, to seed of said spinach plants, and to harvested leaves of said spinach plants. The invention also relates to use of said spinach plants in breeding to confer resistance against Peronospora effusa. The invention further relates to a method for generating a spinach plant having a broad spectrum resistance to Peronospora effusa.

1. The name of the design product: sofa (spinach FB-691). 2. The use of the design product: furniture. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: perspective view 1. 5. The bottom view of the design product is not easily visible or not visible when in use, and the bottom view is omitted.

The invention relates to the technical field of agricultural biological agents, and particularly provides trichoderma harzianum as well as a compound preparation and application thereof. The trichoderma harzianum has a remarkable inhibition effect on six common plant pathogenic bacteria such as sclerotinia sclerotiorum, botrytis cinerea and fusarium oxysporum. The fermentation liquor has a remarkable growth promoting effect on various plants such as pakchoi, spinach and flowering Chinese cabbage when being diluted by 10-100 times, and the fresh weight, the dry weight and the chlorophyll content of the plants can be increased. The compound liquid is formed by compounding trichoderma harzianum fermentation liquor and a hypecoum erectum alkaloid extract with the concentration of 50 ppm, the effective dilution interval of the trichoderma harzianum fermentation liquor in the compound liquid is 10-100 times, and the compound liquid has an inhibition effect on cowpea fusarium wilt germs, promotes growth of cowpeas and nicotiana benthamiana at the same time, achieves integration of disease prevention and growth promotion and has a good application prospect. Wide agricultural application prospects are realized.

The present invention relates to a plant material for use as medicament, wherein the plant material is derived from a plant of the Brassica, Spinacia, or Lactuca genus, genetically modified to express a biologically active recombinant protein and comprises the biologically active recombinant protein as an active ingredient. The invention also relates to a method for producing such a plant material, and to a pharmaceutical composition comprising such a plant material and an orally acceptable support.

The invention discloses a multi-source vegetable outer vesicle composition as well as a preparation method and application thereof. The multi-source vegetable outer vesicle composition is prepared from outer vesicles extracted from spinach leaves, ginger tubers, broccoli buds, bitter gourd fruits and celery stems. The spinach leaves, the ginger tubers, the broccoli flower buds, the bitter gourd fruits and the celery stems are mixed according to the fresh weight ratio of (3-5): (1-2): (2-3): (1-1.5): (2-4), and low-temperature homogenization, centrifugation and ultrafiltration purification are performed to obtain the spinach-ginger-containing beverage. The particle size of the multisource vegetable outer vesicle composition is 100-200 nm, and the multisource vegetable outer vesicle composition comprises transmembrane protein TET8 and membrane anchoring protein ANNEXIN, and further comprises active ingredients of miR159a, miR166a, quercetin and kaempferol. The outer vesicle can target a PPAR gamma / FAS pathway and regulate and control lipolysis and metabolism reprogramming of fat cells; meanwhile, an Nrf2 pathway is activated, free radicals are removed, oxidative stress injury is inhibited, and metabolic diseases such as obesity, type II diabetes mellitus and non-alcoholic fatty liver disease can be effectively improved.

The present invention relates to genomic DNA of Spinacia tetrandra, comprising a first or second genomic DNA fragment encoding a protein conferring resistance to the plant pathogen Peronospora farinosa. The present invention further relates to spinach plants having resistance to the plant pathogen Peronospora farinosa, comprising the first or second genomic DNA fragment. The present invention further relates to methods for providing and identifying spinach plants having resistance to the plant pathogen Peronospora farinosa. The present invention also relates to the use of one or more nucleic acid sequences or amino acid sequences for providing or identifying plants having resistance to the plant pathogen Peronospora farinosa.

The present invention relates to the technical field of the biopharmaceutical industry, and specifically to a Platycladus orientalis leaf extract and a preparation method thereof, comprising the following raw materials: a modified Platycladus orientalis leaf extract, a modified total saponin of Panax notoginseng, Panax notoginseng, fibrin, chitosan, iron oxide, glycerol, and a modified spinach solution. In the present invention, the modified Platycladus orientalis leaf extract, the modified total saponin of Panax notoginseng, and the modified spinach solution work together through a synergistic effect. The modified Platycladus orientalis leaf extract contains a relatively high amount of volatile oil, which can stimulate platelet aggregation and promote the release of coagulation factors. The modified total saponin of Panax notoginseng can inhibit excessive platelet activation and aggregation, preventing thrombosis. The modified Platycladus orientalis leaf extract and the modified total saponin of Panax notoginseng can jointly promote platelet aggregation and the release of coagulation factors to accelerate blood coagulation. The modified spinach solution promotes the synthesis and release of coagulation factors by providing vitamin K, thereby accelerating blood coagulation. The three form a dynamic equilibrium system, which promotes blood coagulation through a synergistic effect, achieving a hemostatic effect.

The invention discloses a preparation method and application of a leaf vegetable biomass derived nano material, and belongs to the field of nano material preparation. Common leaf vegetable biomass (such as spinach, edible amaranth and leaf mustard) and ferric chloride hexahydrate are used as raw materials, and the low-cost leaf vegetable biomass derived nano material is prepared by a method of firstly dipping and then pyrolyzing. The nano material has peroxidase-like activity and glutathione-like peroxidase activity, shows application prospects in the anti-tumor field, and also provides experimental basis and theoretical support for high-value utilization of leaf vegetable biomass and development of green catalytic materials.

The present invention relates to an allele designated alpha-WOLF 24 which confers resistance to at least one Peronospora farinosa f. sp. spinacea race, wherein the protein encoded by said allele is a CC-NBS-LRR protein that comprises in its amino acid sequence: a) the motif “MAEIGYSVC” (SEQ ID NO: 1) at its N-terminus; and b) the motif “KWMCLR” (SEQ ID NO: 2); and wherein the LRR domain of the protein has in order of increased preference at least 95%, 96%, 97%, 98%, 98.2%, 98.5%, 98.8%, 99%, 100% sequence similarity to SEQ ID NO: 10. The allele when present in a spinach plant confers complete resistance to at least Peronospora farinosa f. sp. spinacea race Pfs: 1, Pfs: 2, Pfs: 5, Pfs: 6, Pfs: 7, Pfs: 9, Pfs: 11, Pfs: 13, Pfs: 15 and Pfs: 17, and does not confer resistance to downy mildew race Pfs: 16.

The invention provides a composition for inhibiting protein carbonylation and application of the composition. The composition of the present application comprises a first component and a second component, the first component comprising an umbelliferae carrots extract, a cruciferae brassica extract, an azaleaceae vaccinium extract, and an amaranaceae spinach extract as active ingredients; the second component comprises tocopheryl acetate as an active ingredient, and the mass ratio of the active ingredient of the first component to the active ingredient of the second component is 1: 20000 to 3.2: 1. By utilizing the composition provided by the invention, cell protein carbonylation caused by oxidative stress can be delayed, so that the aging state of a human body is improved. The main components of the composition are plant extracts, so that the composition is low in skin irritation, safe, effective and environment-friendly, and a new direction is developed for the development of pure natural whitening and anti-aging cosmetics. The invention further provides a preparation method of the composition and application of the composition in preparation of products for inhibiting protein carbonylation.

The present invention relates to an allele designated alpha-WOLF 29 which confers resistance to at least one Peronospora effusa race, wherein the protein encoded by said allele is a CC-NBS-LRR protein that comprises in its amino acid sequence: a) the motif "MAEIGYSVC" at its N-terminus; and b) the motif "KWMCLR"; and wherein the protein has an amino acid sequence according to SEQ ID NO: 11. The allele when present homozygously in a spinach plant confers complete resistance to at least Peronospora effusa race Pe:1, Pe:2, Pe:6, Pe:7, Pe:8, Pe:9, Pe:10, Pe:11, Pe:12, Pe:13, Pe:14, Pe:15 Pe:16, Pe:17, Pe:18, Pe:19 and Pe:20 and does not confer resistance to races Pe:3, Pe:4, and Pe:5.

The present invention relates to downy mildew, and especially downy mildew caused by the plant pathogen Peronospora farinosa, resistant spinach plants (Spinacia oleracea). The present spinach plants include a downy mildew resistance providing genomic fragment from Spinacia tetrandra. Specifically, the present invention relates to spinach plants being resistant to downy mildew, wherein the spinach plant includes a downy mildew resistance providing genomic fragment from Spinacia tetrandra such as spinach plants including in their genomes one or more nucleic acid sequences selected from the group consisting of SEQ ID No. 1, SEQ ID No. 3, SEQ ID No. 5, SEQ ID No. 7, SEQ ID No. 9, SEQ ID No. 11, SEQ ID No. 13, SEQ ID No. 15, SEQ ID No. 17, SEQ ID No. 19, SEQ ID No. 21, SEQ ID No. 23, SEQ ID No. 25, SEQ ID No. 27, SEQ ID No. 29, SEQ ID No. 31, SEQ ID No. 33 and SEQ ID No. 35.

The application discloses a direct organogenesis genetic transformation method based on spinach stem tips and axillary buds, and relates to the technical field of plant genetic transformation. pCAMBIA1305-GUS As a plant expression vector, the Agrobacterium GV3101 (pSoup-p19) is used as a receptor Agrobacterium to establish a stable genetic transformation system of spinach. The application utilizes the regeneration capacity of the spinach stem tip meristem and axillary bud meristem, can improve the regeneration efficiency and transformation efficiency, and significantly shortens the genetic transformation cycle. The system can be used for the function research of key regulation genes of agronomic traits of spinach and the cultivation of excellent spinach varieties.

The invention provides seeds and plants of spinach hybrid SVVB2646. The invention thus relates to the plants, seeds, plant parts, and tissue cultures of spinach hybrid SVVB2646 and to methods for producing a spinach plant produced by crossing such plants with themselves or with another plant, such as a spinach plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to plants, seeds, plant parts, and tissue cultures of spinach hybrid SVVB2646 comprising introduced beneficial or desirable traits.

This invention relates to the field of molecular genetics, disclosing SNP molecular markers associated with the mottled leaf phenotype of spinach and their applications. The SNP molecular markers contain a nucleotide sequence with a G / A polymorphism at position 158522852 bp on spinach chromosome 3; spinach plants with the G base have normal leaves, while those with the A base exhibit mottled chlorotic leaves. This invention utilizes segregating population analysis (BSA) to discover SNPs associated with the mottled leaf phenotype of spinach and develops a KASP primer combination for detecting the molecular markers for assisted breeding. The three KASP primers provided by this invention can detect the mottled leaf phenotype of spinach at the seedling stage, with high throughput and high accuracy. The co-dominant KASP markers provided by this invention facilitate the screening of spinach varieties containing the mottled leaf gene, significantly accelerating the breeding process of spinach varieties containing the potential mottled leaf gene.

The present invention relates to an allele designated alpha-WOLF27 which confers resistance to at least one race of Peronospora farinosa, wherein the protein encoded by said allele is a CC-NBS-LRR protein which comprises in its amino acid sequence: a) the motif "MAEIGYSVC" at its N-terminus; and b) the motif "KWMCLR"; and wherein the LRR domain of said protein has, in order of increasing preference, at least 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5%, 99%, 99.5%, 100% sequence similarity with SEQ ID NO: 10. When homozygously present in a spinach plant, said allele confers full resistance to at least the races Pe:7, Pe:8, Pe:9, Pe:11, Pe:12, Pe:13, Pe:14, Pe:15, Pe:17 of Peronospora farinosa.

A spinacia plant with a low oxalic acid content, in particular a low oxalic acid content that is stable and unaffected by environmental conditions. A method for producing the same, a screening method, and a screening kit. A spinacia plant with a low oxalic acid content having at least one low oxalic acid locus located in a chromosome 4 region; a method for producing the spinacia plant having the low oxalic acid content through cross breeding the spinacia plant having the low oxalic acid content with another spinacia plant. A method for screening spinacia plants with low oxalic acid contents by selecting the spinacia plant having at least one low oxalic acid locus located in the chromosome 4 region. A kit for screening spinacia plants with low oxalic acid contents having a primer or a probe that is specifically bound to the low oxalic acid locus.

The present invention relates to Spinacia tetrandra genomic DNA including a first or a second genomic DNA fragment, wherein the first or the second genomic DNA fragments include genes encoding proteins providing resistance against the plant pathogen Peronospora farinosa. The present invention further relates to spinach plants being resistant to the plant pathogen Peronospora farinosa, including the first or the second genomic DNA fragments. The present invention further relates to methods for providing and to methods for identifying spinach plants being resistant to the plant pathogen Peronospora farinosa. The present invention also relates to the use of one or more nucleic acid sequences or amino acid sequences for providing, or identifying, plants resistant to the plant pathogen Peronospora farinosa.

The present invention relates to genes, coding sequences and / or proteins providing resistance against Peronospora farinosa and especially to genes, coding sequences and / or proteins providing resistance against Peronospora farinosa in a spinach plant. The present invention further relates spinach plants comprising the present resistance genes, coding sequences and / or proteins and to methods for providing and / or identifying Peronospora farinosa resistant spinach plants using the present genes, coding sequences and / or proteins. Specifically, the present invention relates to a resistance gene capable of providing Peronospora farinosa resistance in spinach, wherein the resistance is comprised in the genomic sequence of SEQ ID No. 1 and spinach plants comprising the present resistance gene.

The present invention aims to provide spinach plants that are resistant to downy mildew. [Solution] The present invention provides a spinach plant including a spinach plant or its progeny line identified by accession number FERM BP-22502 or FERM BP-22503. The spinach plant of the present invention exhibits resistance to downy mildew, preferably to one or more selected from downy mildew races Pfs2, Pfs4, Pfs5, Pfs6, Pfs7, Pfs10, Pfs11, Pfs12, Pfs13, Pfs14, Pfs15, Pfs16, Pfs17, Pfs18, and Pfs19.

Provided herein are spinach plants resistant to Peronospora farinosa and Stemphylium vesicarium, and spinach plants additionally resistant to Cucumber Mosaic Virus (CMV). Also provided herein are genomic fragments providing the present resistances and use thereof for identifying spinach resistant to Peronospora farinosa and Stemphylium vesicarium, and spinach plants additionally resistant to Cucumber Mosaic Virus (CMV). Provided herein are a spinach plant resistant to Peronospora farinosa and Stemphylium vesicarium: including a genomic fragment of Spinacia tetrandra located on chromosome 4 between positions 8255074 and 8620598 of the spinach reference genome, the genomic fragment of Spinacia tetrandra lacking a lethal factor and providing Peronospora farinosa resistance and including a genomic fragment located on chromosome 3 and between positions 1177586 and 1271037 of the spinach reference genome providing Stemphylium vesicarium resistance.

The present invention relates to downy mildew, and especially downy mildew caused by the plant pathogen Peronospora farinosa, resistant spinach plants (Spinacia oleracea). The present spinach plants include a downy mildew resistance providing genomic fragment from Spinacia tetrandra. Specifically, the present invention relates to spinach plants being resistant to downy mildew, wherein the spinach plant includes a downy mildew resistance providing genomic fragment from Spinacia tetrandra such as spinach plants including in their genomes one or more nucleic acid sequences selected from the groups consisting of SEQ ID No. 1, SEQ ID No. 3, SEQ ID No. 5, SEQ ID No. 7, SEQ ID No. 9, SEQ ID No. 11, SEQ ID No. 13, SEQ ID No. 15, SEQ ID No. 17, SEQ ID No. 19, SEQ ID No. 21, SEQ ID No. 23, SEQ ID No. 25, SEQ ID No. 27, SEQ ID No. 29, SEQ ID No. 31, SEQ ID No. 33 and SEQ ID No. 35.

The present invention is a downy mildew resistant spinach plant having the downy mildew resistance RTM-1 gene present on chromosome 4 of the tetra spinach line CGN25466:MGK 01; the above recited downy mildew resistant spinach plant wherein the RTM-1 gene is a gene present on chromosome 4 of the tetra spinach line CGN25466:MGK 01 in the range of chr4_7962907 to chr4_8617232; the downy mildew resistant spinach plant of any one recited having resistance to at least race Pfs1, Pfs2, Pfs3, Pfs4, Pfs5, Pfs6, Pfs7, Pfs8, Pfs9, Pfs10, Pfs11, Pfs12, Pfs13, Pfs14, Pfs15, Pfs16, Pfs17, Pfs18, Pfs19, and UA1014 race exhibited by the Pseudoperonospora farinosa f. sp. esculenta strains.

The present application relates to the technical field of agricultural biological agents, and specifically provides a Trichoderma harzianum, a compound preparation thereof and application.The Trichoderma harzianum has a significant inhibitory effect on six common plant pathogenic fungi such as Sclerotinia sclerotiorum, Botrytis cinerea and Fusarium oxysporum.The fermentation liquor thereof has a significant growth-promoting effect on a variety of plants such as Chinese cabbage, spinach and flowering Chinese kale in a dilution range of 10-100 times, and can improve the fresh weight, dry weight and chlorophyll content of the plants.The compound liquid is compounded by the Trichoderma harzianum fermentation liquor and 50 ppm concentration of the extract of angelica polymorpha alkaloids, wherein the effective dilution range of the Trichoderma harzianum fermentation liquor in the compound liquid is 10-100 times, the compound liquid has an inhibitory effect on Fusarium oxysporum, and simultaneously promotes the growth of cowpea and Nicotiana benthamiana, realizes the integration of disease prevention and growth promotion, and has a broad agricultural application prospect.

The invention discloses a spinach non-tissue culture genetic transformation method which comprises the following steps: constructing a plant expression vector containing a target gene, and transferring the plant expression vector into agrobacterium tumefaciens to obtain positive agrobacterium tumefaciens into which recombinant plasmids are transferred; preparing an infection solution by using the positive agrobacterium with the transferred recombinant plasmid, and inoculating the positive agrobacterium to female flowers of spinach in a flower dipping manner; culturing the infected spinach in a dark place for 18-30 hours, and continuously culturing at the temperature of 16-20 DEG C under the conditions of 16-hour illumination / 8-hour darkness to obtain spinach T0 seeds; t0 positive plants are identified by utilizing PCR (Polymerase Chain Reaction) and RT-qPCR, and the target gene is successfully transferred into a spinach genome and is stably inherited to offspring. According to the invention, a simple, efficient and stable spinach non-tissue culture genetic transformation technical system is established in a flower dipping manner.

The present invention relates to a spinach plant comprising an R15 resistance conferring allele, wherein said allele confers resistance to at least Peronospora farinosa f. sp. spinaciae races Pfs:1, Pfs:2, Pfs:3, Pfs:4, Pfs:5, Pfs:6, Pfs:9, Pfs:11, Pfs:12, Pfs:13, Pfs:14, Pfs:15 and isolate UA1014 and does not confer resistance to Peronospora farinosa f. sp. spinaciae race Pfs:7, and wherein said allele is as found in a plant grown from a seed of which a representative sample was deposited with the NCIMB under NCIMB accession number 42466. When the spinach plant comprises the R15 resistance conferring allele heterozygously said allele further confers at least intermediate resistance to Peronospora farinosa f. sp. spinaciae races Pfs:8 and Pfs:10.