The invention relates to a traditional Chinese medicinal composition for treating chicken coccocidiosis and a preparation method of the traditional Chinese medicinal composition. The traditional Chinese medicinal composition is prepared from following crude drugs in parts by weight: 12 to 18 parts of radix dichroae, 12 to 18 parts of agrimonia pilosa, 15 to 21 parts of radix pulsatillae chinensis,12 to 18 parts of herba portulacae, 12 to 21 parts of humifuse euphorbia herb, 6 to 12 parts of cortex phellodendri, 12 to 21 parts of herba artemisiae apiaceae, 12 to 21 parts of radix sophorae flavescentis, 6 to 9 parts of radix et rhizoma rhei, 12 to 18 parts of radix sanguisorbae and 9 to 18 parts of rhizoma imperatae. The preparation method specifically comprises the following steps: firstly, respectively crushing all the crude drugs into 60 to 80 meshes; and then weighing the crude drugs according to formula ratio, uniformly mixing, metering and subpackaging to obtain a finished product. The traditional Chinese medicinal composition prepared by the preparation method, disclosed by the invention, has the advantages of scientific and reasonable formula, obvious therapeutic effects oncoccidia, no residues, no toxic or side effects, low cost, safety and effectiveness.