The invention belongs to the technical field of molecular
traceability of
plant pathogenic bacteria, and discloses a molecular
traceability method of potato stripe
bacteria, which comprises eight specific SNP (
Single Nucleotide Polymorphism) marker combinations located in the 80226-81462 interval of a CaLso
genome, a nested PCR (
Polymerase Chain Reaction) primer group for detecting the marker combinations, and a molecular
traceability kit containing the primer group. The invention relates to a potato stripe
pathogen molecule traceability method. The method comprises the following steps: sample
DNA extraction, qPCR positive screening, nested PCR amplification, SNP site sequencing comparison and
genotype analysis, so as to realize accurate determination of CaLso geographical source and
haplotype. The method can be applied to CaLso
quarantine and intrusion
risk prevention and control of commercial
crop seeds of
Solanaceae or Umbelliferae imported from ports, provides
technical support for monitoring
pathogen propagation paths and formulating targeted prevention and control strategies, and is high in practicability.