48 results about "Collagen fiber" patented technology

The invention provides a collagen nano dispersion preparation as well as a preparation method and application thereof. The preparation method of the collagen nano dispersion preparation comprises the following steps: S1, dissolving type I collagen and type III collagen in an acidic aqueous medium to prepare a collagen solution; s2, regulating and controlling the temperature, the pH value and the ionic strength of the collagen solution, so that the collagen solution is self-assembled; and S3, when the average particle size of the collagen fibers reaches a target range, performing dynamic freezing on the self-assembly system. The preparation and stable dispersion of the nano-scale collagen fiber can be realized without chemical crosslinking, the uniformity, biocompatibility and safety of the product are remarkably improved, and the nano-scale collagen fiber has excellent water-light effects and the like, and can be widely applied to the field of medical cosmetology.

The invention discloses a preparation method of a fallen fruit extract and application of the fallen fruit extract in prevention or treatment of hyperplasia of mammary glands. The extract is prepared by extracting immature fruits (fallen fruits) of a traditional Chinese medicinal material Citrus chachiensis through a water decoction method. Experiments prove that the dropped fruit extract can significantly reduce the height and diameter of mammary gland tissues of a mammary gland hyperplasia model rat, significantly reduce the thickness of mammary glands, effectively improve abnormal deposition of collagenous fibers around acelles, and reverse typical proliferative pathological changes of the mammary gland tissues. The invention provides a safe and effective new candidate medicine or functional food raw material for prevention and treatment of hyperplasia of mammary glands, realizes high-valued utilization of citrus chachiensis fallen fruit resources, and has a good application prospect.

The present application belongs to the field of textile spinning technology, and particularly relates to a low-hairiness high-strength spinning process of collagen fiber and combed pure cotton fiber blending. The spinning process comprises the following steps: putting the screened collagen fiber and combed pure cotton fiber into a cotton blender for preliminary mixing, spraying an antistatic agent and a softening and moisturizing agent on the mixed fiber for pretreatment, feeding the pretreated mixed fiber into an opener to obtain a lap, then feeding the lap into a carding machine to output a sliver, doubling the sliver in a drawing frame to obtain a roving, feeding the roving into a compact Sirospun spinning frame to output a spun yarn, and obtaining a cheese yarn through an automatic winder. The yarn prepared by the method has the advantages of high strength, less hairiness, uniform evenness, and skin-friendly comfort.

The present application relates to a kind of methods for preparing skin-based eutectic gel using chrome-free tanning blank and polymerizable deep eutectic solvent.The method prepares polymerizable deep eutectic solvent with betaine and acrylic acid, dissolves initiator in it to prepare dipping solution, then adds chrome-free tanning blank, stirs for 4-48 hours at 5-30 ℃, then heats chrome-free tanning blank at 40-80 ℃ for 2-12 hours to obtain skin-based eutectic gel.The skin-based eutectic gel prepared by the method inherits the original high mechanical strength of chrome-free tanning blank, and the three-dimensional network of polymer formed in situ forms network interpenetrating structure with the original three-dimensional network of collagen fibers in leather blank, further improves the mechanical strength of skin-based eutectic gel, thereby effectively solving the problems of insufficient network structure stability, poor mechanical strength and deep eutectic solvent leakage of traditional eutectic gel.The raw materials of the present application are easy to obtain, low in price, and the preparation process is simple and easy to scale up.

The application discloses a Leuconostoc intestini and Lactococcus lactis composition and application, belongs to the field of microbial pharmacy, and relates to application of a Leuconostoc intestini and Lactococcus lactis composition to preparation of a drug for treating pulmonary fibrosis diseases; wherein the quantity of the Leuconostoc intestini and the Lactococcus lactis in the composition is equal in terms of colony forming units. Experimental results show that the Leuconostoc intestini and the Lactococcus lactis can effectively improve pulmonary fibrosis when being intervened alone or in combination. It is known to those skilled in the art that excessive deposition of collagen fibers is a core feature of pulmonary fibrosis diseases, and the Leuconostoc intestini and the Lactococcus lactis have a synergistic effect of inhibiting the content of lung tissue collagen in pulmonary fibrosis. Therefore, the Leuconostoc intestini and the Lactococcus lactis composition has a prospect of being developed into a drug for treating pulmonary fibrosis diseases (including idiopathic pulmonary fibrosis and secondary pulmonary fibrosis).

The invention relates to the technical field of electronic skin, in particular to skin-friendly leather for electronic skin and a preparation method thereof.The preparation method of the skin-friendly leather for electronic skin comprises the steps that (1) natural leather is sequentially subjected to deliming liquid soaking, softening enzyme liquid softening treatment and ionic liquid tanning liquid tanning, then washed to be neutral, freeze-dried and then ground to remove an epidermal layer, and the skin-friendly leather for electronic skin is obtained; a collagenous fiber net-shaped substrate is obtained; (2) adding a skin-friendly modifier into deionized water, carrying out ultrasonic dispersion, then adding a conductive component, and continuously carrying out ultrasonic dispersion to obtain a modified solution; and (3) immersing the collagenous fiber net-shaped substrate into the modification liquid, carrying out immersion treatment, draining water, carrying out hot-pressing shaping, and cooling to obtain the skin-friendly leather for the electronic skin. The skin-friendly leather for the electronic skin is excellent in comprehensive performance and has excellent skin-friendly softness, ventilation comfort, stable conductivity and long-acting antibacterial property, and reliable material support is provided for application of the electronic skin in the fields of medical monitoring, intelligent wearing and the like.

The application discloses a red dye solution, a reagent combination and a method for Masson trichrome staining, and belongs to the field of histochemistry technology. The red dye solution comprises 0.4-1.0% of bibracine, 0.4-1.0% of temptation red and 1-2% of acetic acid, and water is used as a solvent, and the red dye solution further comprises 0.05-0.15% of orange G sodium salt; the reagent combination comprises the red dye solution and further comprises a blue dye solution, the blue dye solution comprises 2% of aniline blue, 1-2% of acetic acid, and water is used as a solvent, and the blue dye solution further comprises 0.005-0.03% of fast green, 0.0025-0.015% of brilliant green or 0.005-0.03% of bromocresol green sodium salt. The reagent combination further comprises hematoxylin dye solution, red differentiation agent and blue differentiation agent. The improved red dye solution for Masson trichrome staining provided by the application increases the affinity for muscle fiber dyeing, makes the muscle fiber dyeing more firm, and can still maintain bright red after phosphomolybdic acid differentiation. Meanwhile, the improved blue dye solution makes the collagen fiber dyeing more bright, and the color contrast between the muscle fiber and the collagen fiber is more obvious and easier to distinguish.

Disclosed in the present invention is a limiting expandable aortic valvular conduit. The limiting expandable aortic valvular conduit comprises a preliminary aortic valvular conduit attached with a collagen fiber coating, wherein the preliminary aortic valvular conduit comprises a proximal pipeline, a valve connecting conduit, and a distal conduit. Further disclosed in the present invention is a preparation method therefor. The limiting expandable aortic valvular conduit provided in the present invention is used for Bentall-type surgeries. Moreover, after the bioprosthetic valve deterioration, the limiting expandable aortic valvular conduit provided in the present invention provides the conditions for implanting a bioprosthetic valve of the same size as the original valve via an interventional approach; that is, after the biological valve deterioration, a valve of the same size as the original valve can be implanted via an interventional approach, without opening the chest of a patient to replace the biological valve of the same size.

The application belongs to the technical field of adsorbing materials, and particularly relates to a high-selectivity strontium adsorbing material, a preparation method and application. The preparation method of the high-selectivity strontium adsorbing material comprises the following steps: loading rare earth ions on collagen fibers to obtain an intermediate material; and reacting an oxalate salt and a dimethylamine cation mixed solution with the intermediate material to generate a MOF crystal material with cavities in situ. The application takes dimethylamine cations as exchange ions, and the crystal structure formed by the coordination of rare earth ions and oxalate ions has a cavity size matching the hydrated ion radius of strontium, and has high selectivity to strontium ions, so that the strontium ions can be removed with high selectivity in the presence of various ions.

The invention belongs to the technical field of medicines, and particularly relates to a glutamine metabolism regulation nano drug delivery system for enhancing pancreatic cancer targeting and penetration as well as a preparation method and application of the glutamine metabolism regulation nano drug delivery system. The metabolism regulation medicine is a glutamine transporter and a metabolic enzyme inhibitor; the nano drug delivery system is formed by self-assembly of a multifunctional block polymer and a drug; the polymer has collagen targeting peptide CBP modification, and can target high-abundance collagenous fibers to realize tumor enrichment; the MMP-7 response peptide can respond to high-concentration MMP-7 and trigger size reduction and deep penetration of the drug delivery system; the responsive drug release element can trigger drug release in a tumor intracellular reduction environment. The constructed nano drug delivery system is high in compatibility and suitable for selective delivery of various metabolic regulation drugs; pancreatic cancer drug accumulation and deep penetration can be remarkably enhanced, non-target tissue leakage is reduced, and accurate drug delivery and metabolism regulation strategy synergism and toxicity reduction are achieved.

The application provides a wool collagen antibacterial fabric and a preparation method, and belongs to the field of functional textile materials and manufacturing process textile technology. The shrink-resistant wool yarn and collagen yarn are used as raw materials, and the collagen yarn is composed of marine fish collagen viscose and cotton fibers. The wool yarn is prepared through the following processes: wool yarn: strip dyeing, rewashing, wool splicing, secondary combing, fore-spinning, spinning, self-winding, doubling, doubling, steaming. The collagen yarn is prepared through the following processes: loose bobbin, dyeing, drying, and reversing the bobbin. Weaving: warping, harnessing, weaving, inspection, and repair. Post-finishing: singeing and dust collection, continuous cooking, drying, tank steaming, finishing, washing, separation, lining cooking, drying, intermediate inspection, finishing, brushing, calendering, tank steaming, and finished product. The advantages of wool and collagen fibers are successfully combined, and through process innovation, a multifunctional fabric with antibacterial, moisturizing, comfortable and high-end texture is created, solving the problems of single function and difficult processing in the prior art.

This invention discloses a pH-responsive MOF nanocomposite chromium-free tanning agent, its preparation method, and its application, belonging to the technical field of chromium-free tanning agents for leather. The method disclosed in this invention involves forming a ligand with a borate ester module through the dehydration condensation of 4-carboxyphenylboronic acid and tartaric acid, and then binding zirconium to the ligand via a solvothermal method to form a MOF tanning agent. Subsequently, zirconium tartrate is loaded onto the MOF, enabling controlled release of zirconium tartrate through a pH-responsive switch in the ligand. This improves the problem of uneven tanning caused by the rapid and strong binding of zirconium ions to collagen fibers, resulting in tanned leather with a shrinkage temperature exceeding 80 °C, thereby effectively improving the quality of zirconium-tanned leather products.

The application provides a preparation method of a collagen in-situ mineralized scaffold simulating a bone matrix, S1: extraction of type I collagen fibers; S2: preparation of a biomimetic mineralization solution: carboxymethyl chitosan (CMC) is placed in deionized water, after stirring and dissolving, K2HPO4 is added, CaCl2.2H2O is added drop by drop, and is fully dissolved and mixed to form a carboxymethyl chitosan / amorphous calcium phosphate biomimetic mineralization solution (CMC / ACP); S3: preparation of a biomimetic collagen scaffold composed of a simulated mineralized bone matrix: the acid-recombined collagen extracted in step S1 is fully mixed with hyaluronic acid (HA) and is placed in a dialysis bag, an external PBS buffer is used to gradually increase the pH value, after self-assembly for a period of time, the CMC / ACP biomimetic mineralization solution prepared in step S2 is added in the dialysis bag and is fully mixed, after mineralization for a period of time, the content of the dialysis bag is centrifuged to remove supernatant, and is repeatedly washed with deionized water, finally, after centrifugation, the precipitate is frozen overnight, and the biomimetic scaffold is finally obtained through freeze-drying. The application provides the preparation method of the collagen in-situ mineralized scaffold simulating the bone matrix.

The invention belongs to the technical field of cellulose fibers, and particularly relates to a preparation method of antibacterial, mildew-proof and deodorant collagen cellulose fibers, which comprises the steps of preparation of a composite collagen assistant, preparation of a cellulose spinning stock solution, spinning and post-treatment. The preparation method comprises the following steps: firstly, carrying out selective oxidation on microcrystalline cellulose to obtain dialdehyde cellulose, and carrying out Schiff base reaction on amino on collagen and aldehyde on dialdehyde cellulose molecules to generate imine bonds; the rare earth ions interact with imine groups in the grafted protein, so that the prepared antibacterial, mildew-proof and deodorizing collagen cellulose fiber has high machining performance, also has the characteristics of antibacterial property, deodorization, mildew resistance, high wet modulus and the like, and is natural, degradable, safe and environment-friendly. Moreover, the collagen content in the collagen fiber prepared by the invention is as high as 30-40wt%, which is far beyond the protein content in the conventional collagen fiber.

The utility model discloses a winding machine for sewing thread preparation, relates to the technical field of sewing thread preparation, and aims to solve the problems that after sewing threads on the winding machine are used up, workers still need to manually replace the sewing threads on a feeding roller one by one, and time and labor are wasted in the replacement process. According to the technical scheme, the winding machine is characterized by comprising a winding machine body and a plurality of feeding rollers located at the top of the winding machine body, a fixed frame is fixedly connected to the winding machine body, the feeding rollers are fixedly connected with the inner wall of the fixed frame, a movable frame is slidably connected to the inner wall of the fixed frame, and a rotating block is rotationally connected into the movable frame. The sewing thread on the connecting roller is driven by the lifting assembly to be conveyed to the feeding roller, so that the effect of automatically replacing the sewing thread on the winding machine is achieved, the operation steps of workers are reduced, the working efficiency is improved, meanwhile, the sewing thread can be made of collagenous fibers, the pulling resistance of the sewing thread can be improved, and the production cost is reduced. And the phenomenon that the sewing thread is pulled apart when being wound is prevented.

The invention provides bionic composite hydrogel and a preparation method and application thereof, and belongs to the technical field of medical materials.The bionic composite hydrogel is obtained by mixing CS-MA-GA dispersion liquid and SIS / nHAP dispersion liquid and then conducting a photo-crosslinking reaction, and CS-MA-GA is prepared through a reaction of methacrylic acid MA, chitosan CS and gallic acid GA; the SIS / nHAP is prepared from SIS collagen and hydroxyapatite. Gallic acid in CS-MA-GA and SIS collagen in the bionic composite hydrogel prepared by the invention construct a dual bonding mechanism, so that the material can be firmly and permanently combined with teeth in a humid oral environment, and micro-leakage is effectively prevented. The nano-hydroxyapatite plays a role in reinforcing the filler in a photo-crosslinking network, so that the compression modulus and the wear resistance of the hydrogel are remarkably improved, and the hydrogel can bear the occlusal force. Meanwhile, the SIS collagenous fibers are interwoven with the nHAP and CS-MA-GA network to form a bionic organic-inorganic composite structure, and the composition of natural dentin is highly simulated.

The invention relates to the technical field of materials, in particular to a hydrothermal reaction system of nanofibers as well as a preparation method and application of the hydrothermal reaction system. The reaction system comprises: a calcium source containing calcium ions; a phosphorus source containing phosphorus ions; a doping source containing lanthanum ions; the invention discloses a structure-directing agent capable of being selectively adsorbed on a hydroxyapatite crystal face under an alkaline condition and inducing one-dimensional directional growth. And an alkaline regulator. According to the preparation method disclosed by the invention, crystallization growth is directly carried out under a hydrothermal condition, and a high-temperature calcination step is not needed, so that the problems of fiber breakage, fusion and morphology collapse caused by macromolecular template decomposition and rapid crystal sintering are fundamentally avoided. The prepared nanofiber has ideal morphology with good continuity, uniform diameter and high length-diameter ratio, and can simulate the microstructure of natural collagen fiber more truly, thereby providing a more excellent biological microenvironment for cells.

The invention discloses a photo-thermal oil absorption material with a graded capillary network and a preparation method of the photo-thermal oil absorption material, and relates to the technical field of adsorption materials. The bi-component ethylene-propylene fiber and the collagenous fiber are compounded by adopting an in-situ thermal bonding process to obtain the photo-thermal oil absorption material, so that the collagenous fiber rich in fine capillaries in the photo-thermal oil absorption material is wound on the surface of the bi-component ethylene-propylene fiber with relatively large pores to form a graded capillary network; boron nitride nanosheets are directionally stacked and continuously anchored on the surface of the bi-component ethylene-propylene fiber to form a large-area continuous heat conduction channel, and the photothermal conversion efficiency and the heat conductivity of the photothermal oil absorption material are synergistically enhanced by adding the carbon nanoparticles; the heat on the upper surface of the photo-thermal oil absorption material is quickly transferred to an oil-material interface to reduce the viscosity of oil, so that the adsorption performance of the photo-thermal oil absorption material on the oil is further improved.

The invention discloses a winding device and method based on blending of collagen fibers and Moire fibers, and relates to the technical field of textile. The winding device comprises a lower shell, an upper shell is fixedly connected to the top of the lower shell, a heat loss prevention mechanism is arranged in the lower shell, a receding mechanism is arranged in the upper shell, and the lower shell is fixedly connected with the upper shell. A cleaning mechanism is arranged on the upper shell, the heat loss prevention mechanism comprises a plurality of conveying rollers rotationally connected to the inner wall of the lower shell, parts contained in the conveying rollers are the same, and a plurality of first telescopic rods are fixedly connected to the inner walls of the conveying rollers. The yarn generates pressure on the conveying roller, if the pressure is too large, the surface integrity of collagen fibers can be affected, at the moment, the tension change of the yarn can act on the floating plate, the floating plate moves downwards and extrudes the first telescopic rod and the first spring, and therefore the contact pressure and friction force between the yarn and the conveying roller are dynamically reduced.

A method for treating regenerated collagen fibers, which comprises the following step (i). Step (i): a step for impregnating regenerated collagen fibers having a fiber swelling ratio in water of 200% or more as calculated by formula (1) in a fiber treatment agent containing component (A): (A) a copolymer containing a structural unit derived from an unsaturated monomer having a carboxyl group or a salt thereof and a structural unit derived from an aromatic vinyl compound; the copolymer has an acid value of 100 mgKOH / g or more and a weight average molecular weight of 1500-15000. In the formula, w1 represents the mass (g) of the fiber measured by putting 1.0 g of the fiber into a container filled with 100 g of ion-exchanged water, sealing the container, immersing the container in a water bath at 40 DEG C for 30 minutes, taking out the fiber from the container, and centrifugally dewatering for 1 minute at a centrifugal force of 220 [* g], and w2 represents the mass (g) of the fiber measured by putting the fiber with the mass (w1) measured on a filter paper, and measuring the mass of the fiber after drying at 105 DEG C for 3 hours).

The invention provides a method for evaluating mutual adsorption performance of enzyme and animal skin collagen fibers. The method is characterized by comprising the following steps: degreasing, drying and crushing an animal skin reticular layer to obtain fibrous animal skin collagen powder, filtering the fibrous animal skin collagen powder with a 10-200-mesh sieve, weighing 0.01-1.00 g of the fibrous animal skin collagen powder, dispersing the fibrous animal skin collagen powder in 1-20 mL of ultrapure water at the temperature of 0-10 DEG C, carrying out constant-temperature oscillation / stirring at the temperature of 0-10 DEG C for 2-12 h, then adjusting the pH value to 1.0-14.0 with an acid or alkali solution, continuing to carry out constant-temperature oscillation or stirring for 30-360 min until the pH value is stable, and thus obtaining the collagen powder. And adding a proper amount of enzyme solution, oscillating at constant temperature for 0.5-12 hours, freezing and centrifuging for 1-30 minutes under the condition of 1000-6000 rpm, taking supernate, measuring the total protein concentration, comparing with a blank sample, and checking the affinity of the enzyme and the collagen fiber. The method has important guiding significance on animal skin processing biotechnology development.

The application provides a kind of polyphosphate or polyphosphoric acid containing calcium phosphate nanoparticles and its preparation method and application in hard tissue repair products, the diameter of the calcium phosphate nanoparticles is 1nm-10μm, the mass fraction of polyphosphate or polyphosphoric acid in calcium phosphate nanoparticles is 10-80%; it is obtained by mixing calcium-containing solution and phosphorus-containing solution, the phosphorus-containing solution includes polyphosphate or polyphosphoric acid and phosphate or phosphoric acid, the concentration of polyphosphate or polyphosphoric acid in the phosphorus-containing solution is 0.1-500mg / mL.The polyphosphate or polyphosphoric acid containing calcium phosphate nanoparticles obtained by the application has the advantages of small size, controllable particle size and high biological safety, the preparation method is simple to operate, low in cost, and can realize large-scale preparation of calcium phosphate nanoparticles;And can effectively mineralize collagen fibers, and repair hard tissues such as bone and enamel repair, has a wide application prospect.

The present application relates to a kind of Csi-Bantam inhibitor and its application in the preparation of the drug for treating liver fibrosis caused by Clonorchis sinensis infection, belong to biological manufacturing technical field, the present application has found that Csi-Bantam analog can be combined with the 3'-UTR region of Trim35 gene, by inhibiting the function of Csi-Bantam further blocks its PI3K / Akt signal pathway, can significantly inhibit the activation of hepatic stellate cell (activated hepatic stellate cell is the core driving cell of liver fibrosis, is also the main source of extracellular matrix) and the excessive secretion of type I, type III collagen fiber and other extracellular matrix, to effectively improve the process of liver fibrosis.The present application first discloses the new use of Csi-Bantam inhibitor in the preparation of liver fibrosis treatment drug.This discovery provides a new idea for the targeted treatment of liver fibrosis, and has important clinical transformation value for the treatment of liver fibrosis caused by parasitic infection and other factors.

The present application provides a collagen fiber interpenetrating hyaluronic acid or salt composite gel, a preparation method and application thereof, in which the collagen fibers are uniformly dispersed in the inside and outside of the cross-linked hyaluronic acid or salt gel, forming a collagen fiber and hyaluronic acid or salt gel interpenetrating double cross-linked network structure. The collagen fiber interpenetrating hyaluronic acid or salt composite gel has more excellent mechanical strength, supporting performance and anti-degradation performance, can fully exert the respective advantages of hyaluronic acid and collagen, solve the technical problems such as easy precipitation of the mixture of the two in the prior art, swelling of the product after injection, and the like, and the fibrous collagen is closer to the structure of collagen in the human body, has a better cell adhesion and proliferation effect, and promotes cell ingrowth and tissue regeneration.

This invention relates to a method for preparing iodine adsorbent materials using zeolite tanned leather scraps. The method uses zeolite tanned leather scraps as raw material, which are activated with alkali at 20-60°C for 2-10 hours. Then, a nitrogen-containing modifier and a dialdehyde crosslinking agent are added to the activated zeolite tanned leather scrap dispersion, and the mixture is stirred at 20-60°C for 4-12 hours. After filtration and drying, the iodine adsorbent material is obtained. This method increases the adsorption active sites of collagen fibers in the zeolite tanned leather scraps through graft modification. Relying on the synergistic effect of "collagen fiber-zeolite," it combines the advantages of physical, chemical, and ion exchange adsorption, exhibiting large adsorption capacity, fast adsorption rate, and strong selectivity. It can be used for the adsorption of gaseous / liquid iodine. This method is both a green treatment technology for zeolite tanned leather scraps and provides a highly efficient adsorbent material for the remediation of radioactive iodine pollution, aligning with the circular economy and green development concept of "treating waste with waste." This invention has a simple process, widely available raw materials, low cost, and is suitable for large-scale production.

The utility model provides a non-woven fabric facial mask made of collagen fiber, which relates to the technical field of cosmetics and comprises a facial mask main body, the facial mask main body comprises an upper court area, a middle court area and a lower court area, and eye three-dimensional films, nose three-dimensional films and lip three-dimensional films are arranged on the surface edges of eye grooves, nose grooves and lip grooves. A C-shaped cutting line is arranged at the connecting edge of the atrium area and the upper court area, a C-shaped cutting line is arranged at the connecting edge of the atrium area and the lower court area, and lifting belts are arranged at the edges of the two ends of the atrium area, the upper court area and the lower court area. The contact area and the compactness between the mask and the skin are effectively improved, the lifting belt can be easily pulled up, the tightness of the mask is adjusted according to personal requirements, the upward lifting feeling of the skin is effectively promoted, a large amount of water can be locked through the three-spiral structure of collagen, the water can be highly locked, the water content of the skin can be remarkably improved through the mask, and the skin can be effectively protected. The loss of skin moisture is reduced, and efficient moisturizing is realized.

The utility model discloses a kind of fish leather product production are used to remove fishy smell device, comprising: soaking tank, central position is equipped with rotary seat, the rotary seat has fixed part and rotary part, the central position of the rotary part is equipped with water guide channel;Water guide column is equipped with water guide cavity, the water guide cavity is communicated with the water guide channel, the water guide cavity is equipped with several water guide ports;Water guide pipe is inserted in the water guide port, pipe wall is equipped with several through holes;Circulating water tank is equipped with first pipeline and second pipeline, first pipeline and second pipeline communicate the soaking tank and the circulating water tank constitute circulating water path, the circulating water tank is also equipped with third pipeline, the third pipeline passes through the fixed part and is communicated with the water guide channel.The soaking liquid of the utility model can fully contact the inner wall of tubular fish leather, and the fishy smell substance between collagen fiber gaps is released by water flow impact, which greatly improves the removal rate of fishy smell substance and solves the problem that the inner wall of fish leather is difficult to reach in traditional soaking.

The invention relates to the technical field of biology, in particular to an economical collagenous fiber dyeing kit and a preparation method thereof, and the economical collagenous fiber dyeing kit comprises the following reagents: modified iron hematoxylin, a Cole hematoxylin solution, a sirius red dyeing solution, a modified eosin dyeing solution, a modified fuchsin solution, a differentiation concentrated solution, an acidic differentiation solution, a bluing solution and a Scott bluing promoting solution. According to the invention, the modified iron-hematoxylin a liquid can enhance the solubility of the dye, delay oxidative browning and prevent precipitation, so that the nuclear dyeing is clearer and more uniform, and the modified iron-hematoxylin b liquid can stabilize the release of iron ions, reduce the excessive corrosion of hydrochloric acid and enhance the dyeing contrast; the mixing of the two ensures that cell nucleus staining has excellent selectivity, distinct contrast ratio and good reproducibility; the modified eosin dye liquor can maintain the pH stability of the dye liquor and enhance the permeability and affinity to cytoplasm; the modified fuchsin solution can rapidly form a uniform and stable working solution, and the dyeing effects of high specificity and distinct contrast on collagenous fibers are achieved.

The application belongs to the technical field of biological medicine, and particularly relates to a recombinant collagen repair solution and a preparation method and application thereof. The application comprises the following steps: preparation of a protein activity enhancer; preparation of a recombinant humanized collagen fiber sol; and photo-polymerization construction of a recombinant humanized collagen hydrogel. The application is modified by sulfate esterification of pollen of apiaceae polysaccharide, and is compounded with the recombinant humanized collagen. The application can not only form a stable complex with the positively charged collagen amino acid residues through electrostatic interaction, maintain the natural triple helix conformation, reduce the aggregation and denaturation of the collagen, and enhance the activity of the collagen, but also can assist the recombinant humanized collagen in activating the cell surface receptors, enhancing the adhesion and proliferation of the cells, thereby enhancing the epidermal regeneration capacity of the recombinant humanized collagen, effectively improving the bioavailability of the recombinant humanized collagen at the wound site, and improving the repair and treatment effect on the wounded skin.

This invention belongs to the field of biomedical materials technology, specifically relating to a highly biocompatible and highly stable collagen fiber, its preparation method, and its application. The method includes the following steps: (1) constructing collagen fibers; (2) cross-linking collagen fibers with a cross-linking agent; (3) dialysis, homogenization, centrifugation, and collection of the precipitate. The prepared cross-linked collagen fibers are tightly packed and highly cross-linked, exhibiting good thermal stability and resistance to enzymatic degradation. The cross-linked collagen fibers have high biocompatibility, promoting fibroblast proliferation; they have high biocompatibility, and the inflammatory response caused after implantation is significantly mild and quickly eliminated. No calcification nodes appear after implantation of the cross-linked collagen fibers, demonstrating excellent anti-calcification effects. The cross-linked collagen fiber implant provided by this invention significantly promotes the production of new collagen and can be applied in fields such as implants, artificial skin, hemostatic sponges, scaffold materials, and medical devices, with broad application prospects.