95 results about "Collagen fiber" patented technology

The invention discloses a three-dimensional biological printing meniscus model for simulating the mechanical heterogeneity of a natural meniscus and a preparation method of the three-dimensional biological printing meniscus model. The model comprises a structural PCL fiber frame (red region fiber circumferential arrangement and white region radial arrangement, and simulates the trend of natural collagen fiber), regional specific hydrogel (the red region contains GelMA, HAMA and SilMA, and the white region contains GelMA and HAMA), and partitioned cell components (the red region contains BMSC, HUVEC and CTGF, and the white region contains BMSC and TGF-beta3), the PCL frame and the hydrogel cooperate to form a mechanical gradient, and the compression modulus from the red region to the white region is simulated to continuously change. The preparation method comprises the following steps: designing a three-dimensional model, printing a PCL framework, preparing hydrogel and a cell suspension, preparing bio-ink, filling and printing by multiple nozzles, curing and culturing in vitro to obtain a target model. Compared with the prior art, the method has the advantages that the key problem of mechanical mismatch of an existing support is effectively solved, the model better fits a natural meniscus in mechanical property and biological function, and a more adaptive in-vitro model support is provided for individualized meniscus repair and transplantation substitution research.

The invention provides an in-situ tissue engineering valve stent material as well as a preparation method and application thereof, and belongs to the technical field of medical materials. According to the invention, the surface of the acellular matrix is loaded with the super-particle coating formed by self-assembly of the polyphenol-mediated polypeptide aggregate and the Prussian blue nano-enzyme, so that the stent material is endowed with double ROS regulation and endothelial cell adhesion promoting functions, excessive deposition of collagenous fibers can be prevented, and fibrosis of valve tissues is avoided; the synergistic effect of the components of the super-particle coating promotes the endothelialization process of the stent and the remodeling of a functional extracellular matrix, so that the accelerated formation of the functional endodermis is finally realized, and a powerful guarantee is provided for the success of clinical transformation of an in-situ heart valve tissue engineering technology.

The invention provides a collagen nano dispersion preparation as well as a preparation method and application thereof. The preparation method of the collagen nano dispersion preparation comprises the following steps: S1, dissolving type I collagen and type III collagen in an acidic aqueous medium to prepare a collagen solution; s2, regulating and controlling the temperature, the pH value and the ionic strength of the collagen solution, so that the collagen solution is self-assembled; and S3, when the average particle size of the collagen fibers reaches a target range, performing dynamic freezing on the self-assembly system. The preparation and stable dispersion of the nano-scale collagen fiber can be realized without chemical crosslinking, the uniformity, biocompatibility and safety of the product are remarkably improved, and the nano-scale collagen fiber has excellent water-light effects and the like, and can be widely applied to the field of medical cosmetology.

The present invention discloses a preparation method of a leather tanning agent based on modified convexobar crystals, specifically: γ-glycidyl ether propyl trimethoxysilane, chitosan, triethylamine, and deionized water are sequentially added to a convexobar crystal solution, stirred to obtain a mixed solution; hydrochloric acid is added to the obtained mixed solution to adjust the pH to 4-6, and then freeze-dried to obtain a solid powder, which is a leather tanning agent modified by convexobar crystals. The present invention stably combines the epoxy groups on the convexobar crystals modified with KH-560 through the amino groups in chitosan, and the free epoxy groups exert a tanning effect and cross-link with collagen fibers, thereby greatly improving the shrinkage temperature of the leather. In addition, the present invention proposes a "gradient feeding in-situ one-step preparation strategy" to modify the convexobar crystals, which is environmentally friendly and simple, and realizes the sustainable development of chrome-free tanning technology.

The invention discloses a preparation method of a fallen fruit extract and application of the fallen fruit extract in prevention or treatment of hyperplasia of mammary glands. The extract is prepared by extracting immature fruits (fallen fruits) of a traditional Chinese medicinal material Citrus chachiensis through a water decoction method. Experiments prove that the dropped fruit extract can significantly reduce the height and diameter of mammary gland tissues of a mammary gland hyperplasia model rat, significantly reduce the thickness of mammary glands, effectively improve abnormal deposition of collagenous fibers around acelles, and reverse typical proliferative pathological changes of the mammary gland tissues. The invention provides a safe and effective new candidate medicine or functional food raw material for prevention and treatment of hyperplasia of mammary glands, realizes high-valued utilization of citrus chachiensis fallen fruit resources, and has a good application prospect.

The invention relates to the technical field of neutrophile granulocyte separation, in particular to a mouse neutrophile granulocyte separation method which comprises the following steps: (1) anesthetizing and disinfecting a mouse; (2) tissue sampling; (3) tissue digestion; (4) preparing a cell suspension; (5) purifying cells; (6) cell culture; according to the method disclosed by the invention, isoflurane is adopted in the anesthesia disinfection step, so that central nervous activity of mice can be rapidly inhibited, tissue damage caused by stress reaction is avoided, and chemical stimulation can be reduced while body surface microorganisms are cleared through combination of ethanol disinfection and sterile normal saline flushing, and aortic tissues with intact activity are provided for subsequent material taking. In the compound enzyme digestive juice, collagenase I can specifically degrade aortic wall collagenous fibers, trypsin dissociates adhesion connection between cells, neutral protease assists in decomposing elastic fibers, and DNA enzyme I is supplemented to inhibit cell aggregation, so that the contradiction between incomplete digestion and large cell damage of the traditional enzyme juice is effectively solved.

This invention discloses a composite adsorbent material, its preparation method, and its application in uranium adsorption, belonging to the field of materials technology. The method includes the following steps: S1: A raw material containing collagen fibers and a metal salt is subjected to reaction I to obtain a metal ion / collagen fiber composite; the metal is a transition metal; S2: A mixture containing the metal ion / collagen fiber composite from step S1, a sulfide, and a surfactant is subjected to reaction II to obtain a collagen fiber-based nano-metal sulfide composite adsorbent material. This invention combines collagen fibers with nano-metal sulfide materials, solving the problems of difficult recovery and low reusability of nano-metal sulfide materials when removing radioactive ions from water alone, while also overcoming the low adsorption capacity of collagen fibers alone. This composite material is a highly efficient remover of UO2 from aqueous solutions. 2+ A novel adsorbent material with a maximum adsorption capacity of 300–400 mg / g.

The application belongs to the technical field of polymer composites, and provides a high-strength anti-fatigue continuous aramid fiber / unsaturated polyester resin composite material and a preparation method thereof. The high-strength anti-fatigue continuous aramid fiber / unsaturated polyester resin composite material is prepared by taking unsaturated polyester resin as a matrix, taking polyethylene imine-polydopamine modified continuous aramid fiber as a reinforcing body, and using Michael addition reaction and Schiff base reaction of polydopamine and polyethylene imine to graft polydopamine and polyethylene imine onto the continuous aramid fiber, and at the same time, the modified continuous aramid fiber is arranged in the collagen fiber arrangement structure of the dense bone, so that the high-strength anti-fatigue continuous aramid fiber / unsaturated polyester resin composite material is prepared. The interfacial bonding strength reaches 40.60 MPa, the tensile strength reaches 959.07 MPa, the fatigue life under 600 MPa reaches 760,000 times, the fatigue life under 500 MPa reaches 10,470,000 times, and the fatigue life under 400 MPa reaches 87,100,000 times.

The present application belongs to the field of textile spinning technology, and particularly relates to a low-hairiness high-strength spinning process of collagen fiber and combed pure cotton fiber blending. The spinning process comprises the following steps: putting the screened collagen fiber and combed pure cotton fiber into a cotton blender for preliminary mixing, spraying an antistatic agent and a softening and moisturizing agent on the mixed fiber for pretreatment, feeding the pretreated mixed fiber into an opener to obtain a lap, then feeding the lap into a carding machine to output a sliver, doubling the sliver in a drawing frame to obtain a roving, feeding the roving into a compact Sirospun spinning frame to output a spun yarn, and obtaining a cheese yarn through an automatic winder. The yarn prepared by the method has the advantages of high strength, less hairiness, uniform evenness, and skin-friendly comfort.

The invention discloses a method for preparing a collagen fiber hemostatic material capable of rapidly absorbing water, expanding and rebounding by a directed self-assembly method, which comprises the following specific steps: S1, preparing a collagen solution: taking a collagen raw material, stirring and dissolving by using an acetic acid solution, and adding sodium chloride to prepare the collagen solution; s2, electrochemical assembly: carrying out electrochemical self-assembly after carrying out centrifugal treatment on the collagen solution in the step S1 to prepare a collagen membrane material; s3, cross-linking: carrying out cross-linking on the collagen membrane material in the step S2 under an ultraviolet irradiation condition; and S4, freeze-drying: carrying out gradient low-temperature freeze-drying on the collagen membrane material subjected to cross-linking in the step S3 to obtain a finished product. Sodium chloride in the prepared material has a charge shielding effect, reduces the electrification property of collagen and a stacked compact structure of assembled fibers, so that a porous state is formed on the surface of the collagen membrane subjected to electrochemical assembly; and the porous capillary effect is utilized to quickly absorb water.

The invention discloses a leather synchronous tanning, printing and dyeing method based on environment-friendly bio-based dye, relates to the technical field of leather processing, is particularly suitable for production of clothing leather, and comprises the steps of leather blank pretreatment, preparation of environment-friendly bio-based dye tanning treatment liquid, synchronous tanning, printing and dyeing and post-treatment. According to the method, two independent procedures of traditional tanning and dyeing are combined into a whole, so that the production period is greatly shortened, equipment occupation, water consumption, energy consumption and labor cost are reduced, and the production efficiency is improved; in the whole process chain, the use of a chrome tanning agent is avoided, the pollution risk of toxic and harmful substances such as heavy metal chromium is eliminated from the source, and the load of wastewater treatment and the impact on the environment are greatly reduced. The method is not only suitable for common cowhide, sheepskin and the like, but also suitable for high-grade clothing leather, soft leather and other leather types with high hand feeling requirements due to mild treatment conditions and effective protection on collagenous fibers.

The present application relates to a kind of methods for preparing skin-based eutectic gel using chrome-free tanning blank and polymerizable deep eutectic solvent.The method prepares polymerizable deep eutectic solvent with betaine and acrylic acid, dissolves initiator in it to prepare dipping solution, then adds chrome-free tanning blank, stirs for 4-48 hours at 5-30 ℃, then heats chrome-free tanning blank at 40-80 ℃ for 2-12 hours to obtain skin-based eutectic gel.The skin-based eutectic gel prepared by the method inherits the original high mechanical strength of chrome-free tanning blank, and the three-dimensional network of polymer formed in situ forms network interpenetrating structure with the original three-dimensional network of collagen fibers in leather blank, further improves the mechanical strength of skin-based eutectic gel, thereby effectively solving the problems of insufficient network structure stability, poor mechanical strength and deep eutectic solvent leakage of traditional eutectic gel.The raw materials of the present application are easy to obtain, low in price, and the preparation process is simple and easy to scale up.

The invention discloses a foaming agent pharmaceutical composition for treating varicose veins of lower limbs, which is composed of the following components: 6 ml of concentrated sodium chloride, 10 mg of sodium aescinate, one vitamin C (2 ml: 0.5 g) and 4 ml of air, according to the foaming agent pharmaceutical composition for treating varicose veins of lower limbs and a preparation and use method thereof, a foaming agent is formed through mixing and pushing of air and a solution, and the foaming agent is used for treating varicose veins of lower limbs. The contact area of the medicine and the blood vessel wall is obviously increased, the acting time is prolonged, the closing function of a vein valve can be repaired, a broken and reduced collagen fiber layer on the outer layer of the blood vessel can be repaired, the strength of the blood vessel wall is enhanced, and the effect of treating varicosity is improved.

The application discloses a Leuconostoc intestini and Lactococcus lactis composition and application, belongs to the field of microbial pharmacy, and relates to application of a Leuconostoc intestini and Lactococcus lactis composition to preparation of a drug for treating pulmonary fibrosis diseases; wherein the quantity of the Leuconostoc intestini and the Lactococcus lactis in the composition is equal in terms of colony forming units. Experimental results show that the Leuconostoc intestini and the Lactococcus lactis can effectively improve pulmonary fibrosis when being intervened alone or in combination. It is known to those skilled in the art that excessive deposition of collagen fibers is a core feature of pulmonary fibrosis diseases, and the Leuconostoc intestini and the Lactococcus lactis have a synergistic effect of inhibiting the content of lung tissue collagen in pulmonary fibrosis. Therefore, the Leuconostoc intestini and the Lactococcus lactis composition has a prospect of being developed into a drug for treating pulmonary fibrosis diseases (including idiopathic pulmonary fibrosis and secondary pulmonary fibrosis).

The invention relates to the technical field of electronic skin, in particular to skin-friendly leather for electronic skin and a preparation method thereof.The preparation method of the skin-friendly leather for electronic skin comprises the steps that (1) natural leather is sequentially subjected to deliming liquid soaking, softening enzyme liquid softening treatment and ionic liquid tanning liquid tanning, then washed to be neutral, freeze-dried and then ground to remove an epidermal layer, and the skin-friendly leather for electronic skin is obtained; a collagenous fiber net-shaped substrate is obtained; (2) adding a skin-friendly modifier into deionized water, carrying out ultrasonic dispersion, then adding a conductive component, and continuously carrying out ultrasonic dispersion to obtain a modified solution; and (3) immersing the collagenous fiber net-shaped substrate into the modification liquid, carrying out immersion treatment, draining water, carrying out hot-pressing shaping, and cooling to obtain the skin-friendly leather for the electronic skin. The skin-friendly leather for the electronic skin is excellent in comprehensive performance and has excellent skin-friendly softness, ventilation comfort, stable conductivity and long-acting antibacterial property, and reliable material support is provided for application of the electronic skin in the fields of medical monitoring, intelligent wearing and the like.

The application discloses a red dye solution, a reagent combination and a method for Masson trichrome staining, and belongs to the field of histochemistry technology. The red dye solution comprises 0.4-1.0% of bibracine, 0.4-1.0% of temptation red and 1-2% of acetic acid, and water is used as a solvent, and the red dye solution further comprises 0.05-0.15% of orange G sodium salt; the reagent combination comprises the red dye solution and further comprises a blue dye solution, the blue dye solution comprises 2% of aniline blue, 1-2% of acetic acid, and water is used as a solvent, and the blue dye solution further comprises 0.005-0.03% of fast green, 0.0025-0.015% of brilliant green or 0.005-0.03% of bromocresol green sodium salt. The reagent combination further comprises hematoxylin dye solution, red differentiation agent and blue differentiation agent. The improved red dye solution for Masson trichrome staining provided by the application increases the affinity for muscle fiber dyeing, makes the muscle fiber dyeing more firm, and can still maintain bright red after phosphomolybdic acid differentiation. Meanwhile, the improved blue dye solution makes the collagen fiber dyeing more bright, and the color contrast between the muscle fiber and the collagen fiber is more obvious and easier to distinguish.

The invention provides a bis (2, 3-dihydroxybenzamide) compound as well as a preparation method and application thereof, and belongs to the technical field of medicines. A series of bis (2, 3-dihydroxybenzamide) compounds which are novel in structure and have a good inhibiting effect on cholestatic liver diseases and hepatic fibrosis are obtained by conducting structural modification on cephalochelatin, and the compounds can remarkably inhibit expression of hepatic stellate cell COL1A1 mRNA in vitro and have a very strong anti-hepatic fibrosis effect; in vivo, the traditional Chinese medicine composition can obviously relieve related biochemical indexes of cholestatic liver disease mouse model liver injury induced by 3, 5-diethoxycarbonyl-1, 4-dihydro-2, 4, 6-trimethylpyridine (DDC), inhibit expression of related genes of liver fibrosis, reduce the content of liver collagenous fibers, inhibit liver inflammation and bile duct reaction, and can be used for preventing and treating cholestatic liver diseases. The traditional Chinese medicine composition can be used for treating cholestatic liver diseases and hepatic fibrosis.

The application provides visible light catalytic cross-linked virus-like ruthenium oxide nanoparticles for efficient transmembrane treatment of keratoconus, which are mainly prepared from cetyltrimethylammonium bromide, sodium hydroxide, tetraethyl orthosilicate, cyclohexane, ruthenium chloride, phenylalanine and pharmaceutically acceptable components or adjuvants, and have virus-like nanoscale and rough morphology. The virus-like ruthenium oxide nanoparticles can efficiently cross the corneal epithelium, enter the corneal stroma, and react with ammonium persulfate to generate free radicals, oxidize the tyrosine groups in the collagen fibers of the cornea to form a di-tyrosine bond, and cross-link the collagen fibers of the cornea. The application does not require ultraviolet light irradiation, is convenient to use, is non-invasive and reduces the discomfort of patients during treatment, and has important significance for improving the compliance and quality of life of keratoconus patients.

The application belongs to the technical field of medicine, and relates to application of X201415 acting on GPR75 protein in preparation of a medicine for treating non-alcoholic fatty hepatitis. Experiments show that the lipid-lowering effect of X201415 in liver cells is exerted through GPR75 protein. After X201415 treatment, the liver morphology of mice is improved, the body weight and liver index of the mice are reduced, and the TC and TG contents of the liver are significantly reduced in CDAHFD-induced NASH mice; in addition, the epididymal fat volume is also significantly reduced. Liver histology shows that X201415 can effectively improve lipid metabolism disorder and the degree of fibrosis, make the liver lobule arrangement more orderly, reduce fat accumulation and collagen fibers; down-regulate the expression level of lipid metabolism related genes, and inhibit the synthesis of fatty acid and triglyceride. At the same time, X201415 significantly inhibits the expression of inflammation factor genes and fibrosis related genes, which reflects the potential efficacy of the medicine in regulating the process of inflammation and fibrosis. The application provides experimental basis and ideas for synthesis of X201415, new targets and application of X201415 in NASH.

The application belongs to the technical field of functional knitted fabric, and discloses a functional knitted fabric containing collagen and a preparation method and application thereof; the functional knitted fabric is knitted from blended yarn into rib color weaving interstrip organization, and a biological antibacterial finishing layer is loaded on the surface of the fabric; the blended yarn includes 10% to 25% collagen fiber, 20% to 40% modal fiber, 20% to 30% cotton fiber, 10% to 20% diacetate fiber and 10% to 15% polyurethane elastic fiber in percentage by weight. The functional knitted fabric has excellent moisture retention, antibacterial property and elasticity, has no risk of chemical antibacterial agent residue, has stable antibacterial performance and color fastness after washing, is suitable for applications in multiple scenes such as smooth skin-friendly close-fitting clothing, sports fabric and the like, and has better comprehensive performance than traditional similar functional knitted fabric.

The invention belongs to the technical field of biological medicines, and particularly relates to a recombinant collagen repair liquid as well as a preparation method and application thereof. Comprising the following steps: preparing a protein activity enhancer; preparing a recombinant humanized collagen fiber sol; and carrying out photopolymerization to construct the recombinant humanized collagen hydrogel. After turnip pollen polysaccharide is subjected to sulfating modification and compounded with recombinant humanized collagen, the turnip pollen polysaccharide and positively charged collagen amino acid residues can form a stable compound through electrostatic interaction, natural triple helix conformation of the turnip pollen polysaccharide is maintained, collagen aggregation denaturation is reduced, and the activity of the turnip pollen polysaccharide is enhanced; in addition, the recombinant humanized collagen can be assisted in activating cell surface receptors and enhancing adhesion and proliferation of cells, so that the epidermal regeneration promoting capacity of the recombinant humanized collagen is enhanced, and the bioavailability of the recombinant humanized collagen at wounds and the repair treatment effect of the recombinant humanized collagen on the wounded skin can be effectively improved.

The invention discloses a traditional Chinese medicine preparation for treating rheumatoid arthritis combined with pulmonary interstitial lesion. The traditional Chinese medicine preparation is prepared from the following raw material medicines in parts by weight: 10-20 parts of honeysuckle stem, 10-20 parts of duchesnea indica, 8-12 parts of zaocys dhumnade, 8-12 parts of semen brassicae, 8-12 parts of frankincense, 10-20 parts of ramulus mori, 10-20 parts of rhizoma dioscoreae nipponicae and 8-12 parts of myrrh. According to the traditional Chinese medicine preparation for treating rheumatoid arthritis combined with pulmonary interstitial lesion, in a collagen-induced arthritis (CIA) model, the gene transcriptional level of Chi3l1 is remarkably reduced, remarkable indexes in CTD-ILD such as Chi3l1 and KL-6 in serum are remarkably reduced, collagen fiber deposition can be remarkably reduced, and the rheumatoid arthritis combined with pulmonary interstitial lesion can be effectively treated. The traditional Chinese medicine composition has a remarkable treatment effect on rheumatoid arthritis combined with pulmonary interstitial lesion (RA-ILD).

The invention provides a novel application of melia diol. The melia diol has a remarkable senescence cell removing capability in a bleomycin induced cell senescence model, an etoposide induced cell senescence model and a replicative cell senescence model; the activity of anti-apoptotic protein Bcl-2 can be obviously inhibited; the neem diol has no obvious liver and kidney toxicity while selectively removing senescent cells; the neem diol has the effects of remarkably lightening wrinkles on the back skin of a photoaged mouse, improving the transdermal moisture loss condition of the back skin of the photoaged mouse, increasing the moisture content of the back skin of the photoaged mouse, reducing the thickness of an epidermal layer and improving the fracture and content of collagenous fibers in a corium layer; the method has a wide application prospect in preparation of anti-aging products.

Disclosed in the present invention is a limiting expandable aortic valvular conduit. The limiting expandable aortic valvular conduit comprises a preliminary aortic valvular conduit attached with a collagen fiber coating, wherein the preliminary aortic valvular conduit comprises a proximal pipeline, a valve connecting conduit, and a distal conduit. Further disclosed in the present invention is a preparation method therefor. The limiting expandable aortic valvular conduit provided in the present invention is used for Bentall-type surgeries. Moreover, after the bioprosthetic valve deterioration, the limiting expandable aortic valvular conduit provided in the present invention provides the conditions for implanting a bioprosthetic valve of the same size as the original valve via an interventional approach; that is, after the biological valve deterioration, a valve of the same size as the original valve can be implanted via an interventional approach, without opening the chest of a patient to replace the biological valve of the same size.

The application belongs to the technical field of adsorbing materials, and particularly relates to a high-selectivity strontium adsorbing material, a preparation method and application. The preparation method of the high-selectivity strontium adsorbing material comprises the following steps: loading rare earth ions on collagen fibers to obtain an intermediate material; and reacting an oxalate salt and a dimethylamine cation mixed solution with the intermediate material to generate a MOF crystal material with cavities in situ. The application takes dimethylamine cations as exchange ions, and the crystal structure formed by the coordination of rare earth ions and oxalate ions has a cavity size matching the hydrated ion radius of strontium, and has high selectivity to strontium ions, so that the strontium ions can be removed with high selectivity in the presence of various ions.

The invention belongs to the technical field of medicines, and particularly relates to a glutamine metabolism regulation nano drug delivery system for enhancing pancreatic cancer targeting and penetration as well as a preparation method and application of the glutamine metabolism regulation nano drug delivery system. The metabolism regulation medicine is a glutamine transporter and a metabolic enzyme inhibitor; the nano drug delivery system is formed by self-assembly of a multifunctional block polymer and a drug; the polymer has collagen targeting peptide CBP modification, and can target high-abundance collagenous fibers to realize tumor enrichment; the MMP-7 response peptide can respond to high-concentration MMP-7 and trigger size reduction and deep penetration of the drug delivery system; the responsive drug release element can trigger drug release in a tumor intracellular reduction environment. The constructed nano drug delivery system is high in compatibility and suitable for selective delivery of various metabolic regulation drugs; pancreatic cancer drug accumulation and deep penetration can be remarkably enhanced, non-target tissue leakage is reduced, and accurate drug delivery and metabolism regulation strategy synergism and toxicity reduction are achieved.

The application provides a wool collagen antibacterial fabric and a preparation method, and belongs to the field of functional textile materials and manufacturing process textile technology. The shrink-resistant wool yarn and collagen yarn are used as raw materials, and the collagen yarn is composed of marine fish collagen viscose and cotton fibers. The wool yarn is prepared through the following processes: wool yarn: strip dyeing, rewashing, wool splicing, secondary combing, fore-spinning, spinning, self-winding, doubling, doubling, steaming. The collagen yarn is prepared through the following processes: loose bobbin, dyeing, drying, and reversing the bobbin. Weaving: warping, harnessing, weaving, inspection, and repair. Post-finishing: singeing and dust collection, continuous cooking, drying, tank steaming, finishing, washing, separation, lining cooking, drying, intermediate inspection, finishing, brushing, calendering, tank steaming, and finished product. The advantages of wool and collagen fibers are successfully combined, and through process innovation, a multifunctional fabric with antibacterial, moisturizing, comfortable and high-end texture is created, solving the problems of single function and difficult processing in the prior art.

This invention discloses a pH-responsive MOF nanocomposite chromium-free tanning agent, its preparation method, and its application, belonging to the technical field of chromium-free tanning agents for leather. The method disclosed in this invention involves forming a ligand with a borate ester module through the dehydration condensation of 4-carboxyphenylboronic acid and tartaric acid, and then binding zirconium to the ligand via a solvothermal method to form a MOF tanning agent. Subsequently, zirconium tartrate is loaded onto the MOF, enabling controlled release of zirconium tartrate through a pH-responsive switch in the ligand. This improves the problem of uneven tanning caused by the rapid and strong binding of zirconium ions to collagen fibers, resulting in tanned leather with a shrinkage temperature exceeding 80 °C, thereby effectively improving the quality of zirconium-tanned leather products.

The application provides a preparation method of a collagen in-situ mineralized scaffold simulating a bone matrix, S1: extraction of type I collagen fibers; S2: preparation of a biomimetic mineralization solution: carboxymethyl chitosan (CMC) is placed in deionized water, after stirring and dissolving, K2HPO4 is added, CaCl2.2H2O is added drop by drop, and is fully dissolved and mixed to form a carboxymethyl chitosan / amorphous calcium phosphate biomimetic mineralization solution (CMC / ACP); S3: preparation of a biomimetic collagen scaffold composed of a simulated mineralized bone matrix: the acid-recombined collagen extracted in step S1 is fully mixed with hyaluronic acid (HA) and is placed in a dialysis bag, an external PBS buffer is used to gradually increase the pH value, after self-assembly for a period of time, the CMC / ACP biomimetic mineralization solution prepared in step S2 is added in the dialysis bag and is fully mixed, after mineralization for a period of time, the content of the dialysis bag is centrifuged to remove supernatant, and is repeatedly washed with deionized water, finally, after centrifugation, the precipitate is frozen overnight, and the biomimetic scaffold is finally obtained through freeze-drying. The application provides the preparation method of the collagen in-situ mineralized scaffold simulating the bone matrix.

The invention belongs to the technical field of cellulose fibers, and particularly relates to a preparation method of antibacterial, mildew-proof and deodorant collagen cellulose fibers, which comprises the steps of preparation of a composite collagen assistant, preparation of a cellulose spinning stock solution, spinning and post-treatment. The preparation method comprises the following steps: firstly, carrying out selective oxidation on microcrystalline cellulose to obtain dialdehyde cellulose, and carrying out Schiff base reaction on amino on collagen and aldehyde on dialdehyde cellulose molecules to generate imine bonds; the rare earth ions interact with imine groups in the grafted protein, so that the prepared antibacterial, mildew-proof and deodorizing collagen cellulose fiber has high machining performance, also has the characteristics of antibacterial property, deodorization, mildew resistance, high wet modulus and the like, and is natural, degradable, safe and environment-friendly. Moreover, the collagen content in the collagen fiber prepared by the invention is as high as 30-40wt%, which is far beyond the protein content in the conventional collagen fiber.