132 results about "Collagen fiber" patented technology

The invention discloses injectable sodium hyaluronate-silk fibroin composite hydrogel and a preparation method and application thereof.The injectable sodium hyaluronate-silk fibroin composite hydrogel is composed of sodium hyaluronate composite gel particles and a sodium hyaluronate solution, and the sodium hyaluronate composite gel particles comprise silk fibroin sponge microspheres and sodium hyaluronate gel particles; the silk fibroin sponge microspheres are loaded on the sodium hyaluronate gel particles, the silk fibroin sponge microspheres are physical crosslinking microspheres, the particle size D50 is 10-30 [mu] m, and the particle size D90 is within 100 [mu] m; the sodium hyaluronate gel particles are chemical cross-linked particles, the particle size D50 is 150-300 microns, and the particle size D90 is within 800 microns. After the sodium hyaluronate gel particles are in contact with tissues, sodium hyaluronate is preferentially degraded, and silk fibroin exposed in the degradation process has better degradation resistance and an effect of stimulating generation of collagen fibers, so that in-situ fixation of the composite gel particles is realized, and an instant and lasting filling effect is achieved; the material is a safe and effective medical beauty filling material.

The invention discloses a preparation method of a polymer filler containing silk fibroin particles, and relates to the technical field of polymer fillers. The silk fibroin gel particles and the concentrated silk fibroin solution are added at the same time, and the mechanical property of the silk fibroin particles is improved through self-enhanced assembly of the concentrated silk fibroin and the silk fibroin gel. The sheep collagen fiber and the sulfhydrylated hyaluronic acid are also added, so that the degradation time can be prolonged, and a scaffold space is provided for tissue regeneration. Meanwhile, hydrogen peroxide is used for crosslinking, and the concentration of the hydrogen peroxide is controlled to be 5-5.5 mmol / L, so that the stability of the material is improved, the biodegradation rate of the material is reduced, and the degradation time is further prolonged.

The limiting expandable aortic valve pipeline comprises an aortic valve preliminary pipeline attached with a collagen fiber coating, and the aortic valve preliminary pipeline comprises a proximal pipeline, a valve connecting pipe and a telecentric pipeline. The invention also discloses a preparation method thereof. The limiting expandable aortic valve pipeline provided by the invention is used for replacing an aortic artificial blood vessel and a Bentall type operation of an aortic valve, and after the artificial bioprosthetic valve declines, the limiting expandable aortic valve pipeline provided by the invention provides a condition for embedding the artificial bioprosthetic valve with the same size as an original valve in an interventional manner, so that the limiting expandable aortic valve pipeline can be used for replacing the Bentall type operation of the aortic valve and the artificial bioprosthetic valve. In this way, after the bioprosthetic valve declines, the valve with the same size as the original valve can be placed in an interventional mode, and the patient does not need to open the chest to replace the bioprosthetic valve with the same size.

The invention discloses a preparation method of a thermo-sensitive collagen-chitosan composite gel and an application of the thermo-sensitive collagen-chitosan composite gel. The preparation method of the thermo-sensitive collagen-chitosan composite gel comprises the following steps: dissolving hydroxybutyl chitosan and / or hydroxypropyl chitosan and recombinant I-type collagen in water at the temperature of 2 to 5 DEG C, so as to obtain the thermo-sensitive collagen-chitosan composite gel, the amino acid sequence of the recombinant I type collagen is an amino acid sequence obtained by repeating a short amino acid sequence as shown in SEQ ID No: 1 (GAPGAPGSQGAPGLQ) for 52 times. In the thermo-sensitive collagen-chitosan composite gel prepared by the preparation method disclosed by the invention, the recombinant I-type collagen is self-assembled to form collagen fibers which are more beneficial to aggregation and solidification of platelets and can act on a wound surface for a longer time, so that the thermo-sensitive collagen-chitosan composite gel has a better hemostatic effect and a better wound surface healing effect.

Disclosed is use of a Histatin-1 polypeptide in promotion of cartilage regeneration or repair. By preparing a combined reagent containing a Histatin-1 polypeptide, a biological scaffold material and the like, mainly a linear Hst1 is loaded on gelatin methacrylate so as to be used for repairing cartilage damage or promoting cartilage growth; meanwhile, disclosed is use of a Histatin-1 polypeptide in promotion of the expression of one or more of bone differentiation indexes, including a collagen fiber, glycosaminoglycan, type II collagen and aggrecan.

The invention discloses a terylene biological patch capable of being used for aorta root enlargement. The terylene biological patch is terylene cloth attached with a collagen fiber coating; the collagen fiber coating is formed by performing first chemical crosslinking and second chemical crosslinking on collagen fibers, and the first chemical crosslinking refers to performing chemical crosslinking on the collagen fibers to obtain the collagen fibers subjected to the first chemical crosslinking; and the second crosslinking is that the collagen fibers subjected to the first chemical crosslinking are attached to internal gaps of the polyester fabric and the surface of the polyester fabric, and a collagen fiber coating is formed through crosslinking. The invention also discloses a preparation method thereof. The polyester biological patch provided by the invention has the advantages that the coating is stable and is not easy to fall off; the permeability is low; degradation is not prone to occurring, and biocompatibility is good; the compliance is good; and the sewing strength is good.

The invention discloses multifunctional mineralized collagen with a uniform layered structure as well as a preparation method and application of the multifunctional mineralized collagen, and particularly relates to the technical field of biomedical materials. The preparation method comprises the following steps: adding soluble calcium salt into a collagen stock solution, adjusting the pH value of a reaction system to 7-9, and reacting; after the reaction is finished, adding low-valence phosphate and deionized water, and reacting; after the reaction is finished, adding a silver ion solution, adjusting the pH value to 7.4-8.0, and carrying out mineralization reaction; after the mineralization is finished, cleaning, centrifuging, freezing and forming, and freezing and drying to obtain the multifunctional mineralized collagen with a uniform layered structure. According to the preparation method, the mineralized collagen which is provided with a mineralized structure which is assembled by apatite and nano-silver particles in a layered manner and is uniformly distributed along collagenous fibers and has good antibacterial performance and osteogenic performance is prepared on the micron scale and the nano-scale by utilizing the reaction of collagen groups and phosphate and introducing silver ions with oxidation and antibacterial performance.

The invention belongs to the technical field of cosmetics, and particularly relates to whitening and freckle-removing essence oil and a preparation method thereof. According to the invention, adamantane formyl is introduced into an alpha-arbutin benzene ring to obtain the adamantane formyl modified alpha-arbutin derivative, so that the compatibility of alpha-arbutin and base oil is enhanced; the alpha-arbutin is prevented from being converted into hydroquinone by utilizing the large steric effect of adamantane formyl, so that the safety of the whitening and freckle-removing essence oil is improved; the tranexamic acid is wrapped by the ceramide nano-emulsion, so that the skin allergy risk caused by ingestion of the tranexamic acid is reduced; the ceramide nano-emulsion and tranexamic acid act together to repair skin collagenous fibers, block melanin transfer and inhibit melanin synthesis; trilinolein and glabridin are catalyzed by lipase to form a compound, so that the molecular compatibility and photo-thermal stability of glabridin are enhanced; glycerin promotes transdermal absorption of glabridin and enhances the efficacy of the whitening and freckle-removing essence oil.

The invention discloses a three-dimensional biological printing meniscus model for simulating the mechanical heterogeneity of a natural meniscus and a preparation method of the three-dimensional biological printing meniscus model. The model comprises a structural PCL fiber frame (red region fiber circumferential arrangement and white region radial arrangement, and simulates the trend of natural collagen fiber), regional specific hydrogel (the red region contains GelMA, HAMA and SilMA, and the white region contains GelMA and HAMA), and partitioned cell components (the red region contains BMSC, HUVEC and CTGF, and the white region contains BMSC and TGF-beta3), the PCL frame and the hydrogel cooperate to form a mechanical gradient, and the compression modulus from the red region to the white region is simulated to continuously change. The preparation method comprises the following steps: designing a three-dimensional model, printing a PCL framework, preparing hydrogel and a cell suspension, preparing bio-ink, filling and printing by multiple nozzles, curing and culturing in vitro to obtain a target model. Compared with the prior art, the method has the advantages that the key problem of mechanical mismatch of an existing support is effectively solved, the model better fits a natural meniscus in mechanical property and biological function, and a more adaptive in-vitro model support is provided for individualized meniscus repair and transplantation substitution research.

The invention provides an in-situ tissue engineering valve stent material as well as a preparation method and application thereof, and belongs to the technical field of medical materials. According to the invention, the surface of the acellular matrix is loaded with the super-particle coating formed by self-assembly of the polyphenol-mediated polypeptide aggregate and the Prussian blue nano-enzyme, so that the stent material is endowed with double ROS regulation and endothelial cell adhesion promoting functions, excessive deposition of collagenous fibers can be prevented, and fibrosis of valve tissues is avoided; the synergistic effect of the components of the super-particle coating promotes the endothelialization process of the stent and the remodeling of a functional extracellular matrix, so that the accelerated formation of the functional endodermis is finally realized, and a powerful guarantee is provided for the success of clinical transformation of an in-situ heart valve tissue engineering technology.

The invention provides a collagen nano dispersion preparation as well as a preparation method and application thereof. The preparation method of the collagen nano dispersion preparation comprises the following steps: S1, dissolving type I collagen and type III collagen in an acidic aqueous medium to prepare a collagen solution; s2, regulating and controlling the temperature, the pH value and the ionic strength of the collagen solution, so that the collagen solution is self-assembled; and S3, when the average particle size of the collagen fibers reaches a target range, performing dynamic freezing on the self-assembly system. The preparation and stable dispersion of the nano-scale collagen fiber can be realized without chemical crosslinking, the uniformity, biocompatibility and safety of the product are remarkably improved, and the nano-scale collagen fiber has excellent water-light effects and the like, and can be widely applied to the field of medical cosmetology.

The present invention discloses a preparation method of a leather tanning agent based on modified convexobar crystals, specifically: γ-glycidyl ether propyl trimethoxysilane, chitosan, triethylamine, and deionized water are sequentially added to a convexobar crystal solution, stirred to obtain a mixed solution; hydrochloric acid is added to the obtained mixed solution to adjust the pH to 4-6, and then freeze-dried to obtain a solid powder, which is a leather tanning agent modified by convexobar crystals. The present invention stably combines the epoxy groups on the convexobar crystals modified with KH-560 through the amino groups in chitosan, and the free epoxy groups exert a tanning effect and cross-link with collagen fibers, thereby greatly improving the shrinkage temperature of the leather. In addition, the present invention proposes a "gradient feeding in-situ one-step preparation strategy" to modify the convexobar crystals, which is environmentally friendly and simple, and realizes the sustainable development of chrome-free tanning technology.

The invention discloses a preparation method of a fallen fruit extract and application of the fallen fruit extract in prevention or treatment of hyperplasia of mammary glands. The extract is prepared by extracting immature fruits (fallen fruits) of a traditional Chinese medicinal material Citrus chachiensis through a water decoction method. Experiments prove that the dropped fruit extract can significantly reduce the height and diameter of mammary gland tissues of a mammary gland hyperplasia model rat, significantly reduce the thickness of mammary glands, effectively improve abnormal deposition of collagenous fibers around acelles, and reverse typical proliferative pathological changes of the mammary gland tissues. The invention provides a safe and effective new candidate medicine or functional food raw material for prevention and treatment of hyperplasia of mammary glands, realizes high-valued utilization of citrus chachiensis fallen fruit resources, and has a good application prospect.

The invention relates to the technical field of neutrophile granulocyte separation, in particular to a mouse neutrophile granulocyte separation method which comprises the following steps: (1) anesthetizing and disinfecting a mouse; (2) tissue sampling; (3) tissue digestion; (4) preparing a cell suspension; (5) purifying cells; (6) cell culture; according to the method disclosed by the invention, isoflurane is adopted in the anesthesia disinfection step, so that central nervous activity of mice can be rapidly inhibited, tissue damage caused by stress reaction is avoided, and chemical stimulation can be reduced while body surface microorganisms are cleared through combination of ethanol disinfection and sterile normal saline flushing, and aortic tissues with intact activity are provided for subsequent material taking. In the compound enzyme digestive juice, collagenase I can specifically degrade aortic wall collagenous fibers, trypsin dissociates adhesion connection between cells, neutral protease assists in decomposing elastic fibers, and DNA enzyme I is supplemented to inhibit cell aggregation, so that the contradiction between incomplete digestion and large cell damage of the traditional enzyme juice is effectively solved.

This invention discloses a composite adsorbent material, its preparation method, and its application in uranium adsorption, belonging to the field of materials technology. The method includes the following steps: S1: A raw material containing collagen fibers and a metal salt is subjected to reaction I to obtain a metal ion / collagen fiber composite; the metal is a transition metal; S2: A mixture containing the metal ion / collagen fiber composite from step S1, a sulfide, and a surfactant is subjected to reaction II to obtain a collagen fiber-based nano-metal sulfide composite adsorbent material. This invention combines collagen fibers with nano-metal sulfide materials, solving the problems of difficult recovery and low reusability of nano-metal sulfide materials when removing radioactive ions from water alone, while also overcoming the low adsorption capacity of collagen fibers alone. This composite material is a highly efficient remover of UO2 from aqueous solutions. 2+ A novel adsorbent material with a maximum adsorption capacity of 300–400 mg / g.

The application belongs to the technical field of polymer composites, and provides a high-strength anti-fatigue continuous aramid fiber / unsaturated polyester resin composite material and a preparation method thereof. The high-strength anti-fatigue continuous aramid fiber / unsaturated polyester resin composite material is prepared by taking unsaturated polyester resin as a matrix, taking polyethylene imine-polydopamine modified continuous aramid fiber as a reinforcing body, and using Michael addition reaction and Schiff base reaction of polydopamine and polyethylene imine to graft polydopamine and polyethylene imine onto the continuous aramid fiber, and at the same time, the modified continuous aramid fiber is arranged in the collagen fiber arrangement structure of the dense bone, so that the high-strength anti-fatigue continuous aramid fiber / unsaturated polyester resin composite material is prepared. The interfacial bonding strength reaches 40.60 MPa, the tensile strength reaches 959.07 MPa, the fatigue life under 600 MPa reaches 760,000 times, the fatigue life under 500 MPa reaches 10,470,000 times, and the fatigue life under 400 MPa reaches 87,100,000 times.

The invention discloses a sodium lignin sulfonate-TGIC composite functional chrome-free tanning agent as well as a preparation method and application thereof. The sodium lignin sulfonate-TGIC composite functional chrome-free tanning agent comprises the following components: triglycidyl isocyanurate, an organic solvent, a sodium lignin sulfonate LS solution and ethanol, the preparation method comprises the following steps: S1, dissolving triglycidyl isocyanurate in an organic solvent or water, and heating to obtain a uniform triglycidyl isocyanurate TGIC solution; s2, adding a sodium lignin sulfonate LS solution into the triglycidyl isocyanurate TGIC solution in the step S1, heating and stirring for reaction to obtain a reaction mixed solution; s3, cooling the reaction mixed solution prepared in the step S2 to room temperature, adding ethanol, precipitating a product, filtering, washing, precipitating, and performing vacuum drying to obtain a solid powdery LST chrome-free tanning agent for leather tanning; the combining capacity of the tanning agent and leather collagen fibers is remarkably improved; the prepared LST tanning agent is environmentally friendly, low in cost and capable of replacing a traditional chrome tanning agent.

The invention relates to a hydrogel material containing laminin, the hydrogel material comprises a hydrogel body and an effective amount of laminin subunit arranged in the hydrogel body, and the laminin subunit is cross-linked with the hydrogel body. The laminin subunit can be added into the hydrogel, so that the polarity of the dental papilla cell of the miniature pig and the differentiation level of the dentin-forming cell are remarkably improved, the differentiation of the dentin-forming cell is directionally regulated and controlled, the differentiation level of the dentin-forming cell is effectively improved, the operation is simple, and the effect is remarkable. In addition, laminin subunits are added into the collagen-based hydrogel, so that the rigidity of the collagen hydrogel is improved, and the arrangement and mechanical strength of collagen fibers in the hydrogel are improved.

The present application belongs to the field of textile spinning technology, and particularly relates to a low-hairiness high-strength spinning process of collagen fiber and combed pure cotton fiber blending. The spinning process comprises the following steps: putting the screened collagen fiber and combed pure cotton fiber into a cotton blender for preliminary mixing, spraying an antistatic agent and a softening and moisturizing agent on the mixed fiber for pretreatment, feeding the pretreated mixed fiber into an opener to obtain a lap, then feeding the lap into a carding machine to output a sliver, doubling the sliver in a drawing frame to obtain a roving, feeding the roving into a compact Sirospun spinning frame to output a spun yarn, and obtaining a cheese yarn through an automatic winder. The yarn prepared by the method has the advantages of high strength, less hairiness, uniform evenness, and skin-friendly comfort.

The invention discloses a method for preparing a collagen fiber hemostatic material capable of rapidly absorbing water, expanding and rebounding by a directed self-assembly method, which comprises the following specific steps: S1, preparing a collagen solution: taking a collagen raw material, stirring and dissolving by using an acetic acid solution, and adding sodium chloride to prepare the collagen solution; s2, electrochemical assembly: carrying out electrochemical self-assembly after carrying out centrifugal treatment on the collagen solution in the step S1 to prepare a collagen membrane material; s3, cross-linking: carrying out cross-linking on the collagen membrane material in the step S2 under an ultraviolet irradiation condition; and S4, freeze-drying: carrying out gradient low-temperature freeze-drying on the collagen membrane material subjected to cross-linking in the step S3 to obtain a finished product. Sodium chloride in the prepared material has a charge shielding effect, reduces the electrification property of collagen and a stacked compact structure of assembled fibers, so that a porous state is formed on the surface of the collagen membrane subjected to electrochemical assembly; and the porous capillary effect is utilized to quickly absorb water.

The invention discloses a leather synchronous tanning, printing and dyeing method based on environment-friendly bio-based dye, relates to the technical field of leather processing, is particularly suitable for production of clothing leather, and comprises the steps of leather blank pretreatment, preparation of environment-friendly bio-based dye tanning treatment liquid, synchronous tanning, printing and dyeing and post-treatment. According to the method, two independent procedures of traditional tanning and dyeing are combined into a whole, so that the production period is greatly shortened, equipment occupation, water consumption, energy consumption and labor cost are reduced, and the production efficiency is improved; in the whole process chain, the use of a chrome tanning agent is avoided, the pollution risk of toxic and harmful substances such as heavy metal chromium is eliminated from the source, and the load of wastewater treatment and the impact on the environment are greatly reduced. The method is not only suitable for common cowhide, sheepskin and the like, but also suitable for high-grade clothing leather, soft leather and other leather types with high hand feeling requirements due to mild treatment conditions and effective protection on collagenous fibers.

The invention discloses a preparation method of a porous carbon adsorbent for treating antibiotics in water, and belongs to the technical field of environmental protection. Comprising the following steps: (1) adding a leather collagen fiber in the tanning field as a carbon source into deionized water to form a mixed solution, adding titanium sulfate under an acidic condition for tanning, obtaining a precipitate through a high-temperature water bath, washing with deionized water, and freeze-drying to obtain a porous carbon adsorbent precursor; (2) performing high-temperature carbonization on the precursor obtained in the step (1) in an inert atmosphere to obtain a compound of the titanium oxide and the carbon material; and (3) etching the compound in the step (2) with hydrofluoric acid, washing with deionized water, and drying to obtain the porous carbon adsorbent. The raw materials are wide in source, the preparation method is simple, and antibiotics in water can be efficiently adsorbed.

The present application relates to a kind of methods for preparing skin-based eutectic gel using chrome-free tanning blank and polymerizable deep eutectic solvent.The method prepares polymerizable deep eutectic solvent with betaine and acrylic acid, dissolves initiator in it to prepare dipping solution, then adds chrome-free tanning blank, stirs for 4-48 hours at 5-30 ℃, then heats chrome-free tanning blank at 40-80 ℃ for 2-12 hours to obtain skin-based eutectic gel.The skin-based eutectic gel prepared by the method inherits the original high mechanical strength of chrome-free tanning blank, and the three-dimensional network of polymer formed in situ forms network interpenetrating structure with the original three-dimensional network of collagen fibers in leather blank, further improves the mechanical strength of skin-based eutectic gel, thereby effectively solving the problems of insufficient network structure stability, poor mechanical strength and deep eutectic solvent leakage of traditional eutectic gel.The raw materials of the present application are easy to obtain, low in price, and the preparation process is simple and easy to scale up.

The invention discloses a research method of 18beta-glycyrrhetinic acid on high-fat daily ration induced mitochondrial function injury mediated liver injury, which comprises the following steps: S1, test design, S2, test sample collection, S3, index determination, S4, test data statistical analysis, and S5, results, and the research method for 18beta-glycyrrhetinic acid (18beta-glyrhetinic acid, 18beta-glyrhetinic acid, 18beta-glyrhetinic acid, 18beta-glyrhetinic acid, 18beta-glyrhetinic acid, 18beta-glyrhetinic acid, 18beta-glyrhetinic acid, 18beta-glyrhetinic acid, 18beta- The liver injury of the micropterus salmoides caused by high fat ration (HF) can be improved by lowering TGF beta1-Smad2 / 3 signals to reduce aggregation of liver collagenous fibers, and the GA can activate Ampk-Pgc1alpha-Sirt3 signal pathways to reduce expression of calcium ion transporters in endoplasmic reticulum and mitochondria, relieve liver mitochondrial injury and reduce the content of ROS (reactive oxygen species), so that the liver injury of the micropterus salmoides caused by high fat ration (HF) can be relieved, and the liver injury of the micropterus salmoides caused by high fat ration (HF) can be relieved. And the HF can increase the activity of liver function related enzymes of the micropterus salmoides and increase liver lipid droplet deposition and collagen aggregation, and after the GA is added, the adverse effect caused by the HF can be obviously improved, so that the HF-induced liver injury of the micropterus salmoides can be relieved.

The invention provides an improved masson three-color dyeing method. The method comprises the following steps: immersing a tissue slice into a Bouin solution for pretreatment mordanting; dropwise adding an iron hematoxylin staining solution on the tissue slice for staining; immersing the tissue slice into a Bobhlet red staining solution for staining; immersing the tissue slices into a phosphomolybdic acid aqueous solution for fiber differentiation; immersing the tissue slice into aniline blue staining fluid for staining; and sealing the slice to obtain the improved tissue slice. According to the method disclosed by the invention, a Bobillet scarlet staining solution is used for replacing a traditional richlet acid staining solution, and a Bouin solution is combined for pre-treating the section, so that the staining effect is improved, and the staining contrast of collagenous fibers and other tissues is more obvious.

The invention discloses a foaming agent pharmaceutical composition for treating varicose veins of lower limbs, which is composed of the following components: 6 ml of concentrated sodium chloride, 10 mg of sodium aescinate, one vitamin C (2 ml: 0.5 g) and 4 ml of air, according to the foaming agent pharmaceutical composition for treating varicose veins of lower limbs and a preparation and use method thereof, a foaming agent is formed through mixing and pushing of air and a solution, and the foaming agent is used for treating varicose veins of lower limbs. The contact area of the medicine and the blood vessel wall is obviously increased, the acting time is prolonged, the closing function of a vein valve can be repaired, a broken and reduced collagen fiber layer on the outer layer of the blood vessel can be repaired, the strength of the blood vessel wall is enhanced, and the effect of treating varicosity is improved.

The application discloses a Leuconostoc intestini and Lactococcus lactis composition and application, belongs to the field of microbial pharmacy, and relates to application of a Leuconostoc intestini and Lactococcus lactis composition to preparation of a drug for treating pulmonary fibrosis diseases; wherein the quantity of the Leuconostoc intestini and the Lactococcus lactis in the composition is equal in terms of colony forming units. Experimental results show that the Leuconostoc intestini and the Lactococcus lactis can effectively improve pulmonary fibrosis when being intervened alone or in combination. It is known to those skilled in the art that excessive deposition of collagen fibers is a core feature of pulmonary fibrosis diseases, and the Leuconostoc intestini and the Lactococcus lactis have a synergistic effect of inhibiting the content of lung tissue collagen in pulmonary fibrosis. Therefore, the Leuconostoc intestini and the Lactococcus lactis composition has a prospect of being developed into a drug for treating pulmonary fibrosis diseases (including idiopathic pulmonary fibrosis and secondary pulmonary fibrosis).

The invention relates to the technical field of electronic skin, in particular to skin-friendly leather for electronic skin and a preparation method thereof.The preparation method of the skin-friendly leather for electronic skin comprises the steps that (1) natural leather is sequentially subjected to deliming liquid soaking, softening enzyme liquid softening treatment and ionic liquid tanning liquid tanning, then washed to be neutral, freeze-dried and then ground to remove an epidermal layer, and the skin-friendly leather for electronic skin is obtained; a collagenous fiber net-shaped substrate is obtained; (2) adding a skin-friendly modifier into deionized water, carrying out ultrasonic dispersion, then adding a conductive component, and continuously carrying out ultrasonic dispersion to obtain a modified solution; and (3) immersing the collagenous fiber net-shaped substrate into the modification liquid, carrying out immersion treatment, draining water, carrying out hot-pressing shaping, and cooling to obtain the skin-friendly leather for the electronic skin. The skin-friendly leather for the electronic skin is excellent in comprehensive performance and has excellent skin-friendly softness, ventilation comfort, stable conductivity and long-acting antibacterial property, and reliable material support is provided for application of the electronic skin in the fields of medical monitoring, intelligent wearing and the like.

The application discloses a red dye solution, a reagent combination and a method for Masson trichrome staining, and belongs to the field of histochemistry technology. The red dye solution comprises 0.4-1.0% of bibracine, 0.4-1.0% of temptation red and 1-2% of acetic acid, and water is used as a solvent, and the red dye solution further comprises 0.05-0.15% of orange G sodium salt; the reagent combination comprises the red dye solution and further comprises a blue dye solution, the blue dye solution comprises 2% of aniline blue, 1-2% of acetic acid, and water is used as a solvent, and the blue dye solution further comprises 0.005-0.03% of fast green, 0.0025-0.015% of brilliant green or 0.005-0.03% of bromocresol green sodium salt. The reagent combination further comprises hematoxylin dye solution, red differentiation agent and blue differentiation agent. The improved red dye solution for Masson trichrome staining provided by the application increases the affinity for muscle fiber dyeing, makes the muscle fiber dyeing more firm, and can still maintain bright red after phosphomolybdic acid differentiation. Meanwhile, the improved blue dye solution makes the collagen fiber dyeing more bright, and the color contrast between the muscle fiber and the collagen fiber is more obvious and easier to distinguish.

The present application provides a preparation method and application of nitrogen-doped biochar material, which belongs to the field of catalytic material manufacturing. The preparation method of nitrogen-doped biochar material comprises the following steps: immersing collagen fibers in a solvent to obtain a first reaction precursor, wherein the pH of the solvent is ≤7; mixing the first reaction precursor with a second reaction precursor containing a nitrogen source and heating the mixture to obtain a reaction intermediate; performing solid-liquid separation on the reaction intermediate to obtain a solid-phase reaction intermediate; heating and carbonizing the solid-phase reaction intermediate to obtain a nitrogen-doped biochar material. The preparation method of the nitrogen-doped biochar material can improve the problems of complex preparation process and high preparation cost in the preparation method of the nitrogen-doped biochar material to a certain extent.