12 results about "CTGF" patented technology

The invention discloses a preparation method of a growth factor gradient bionic scaffold for tendon-bone interface regeneration and repair, and belongs to the technical field of biomedical materials. The composite scaffold is constructed by adopting a coaxial electrospinning and self-assembly technology, and is compounded by a core-shell oriented nanofiber layer and a non-oriented nanofiber layer through a gelatin adhesive to respectively simulate tendon phase and bone phase structures of a tendon-bone interface; a shell layer of the tendon-phase-simulated core-shell oriented nanofiber is a PCL layer containing CTGF, a core layer of the tendon-phase-simulated core-shell oriented nanofiber is a PVA layer containing TGF-beta1, and a bone-phase-simulated non-oriented nanofiber layer is a PCL / Gel co-spun layer containing BMP-2 polylactic acid microspheres and nano-hydroxyapatite. The scaffold can realize secondary controlled release of early-stage release of CTGF, slow release of TGF-beta1 in the middle and later periods and continuous release of BMP-2, and cooperates with a fiber orientation structure to induce graded differentiation of mesenchymal stem cells and promote integrated repair of a tendon-bone interface gradient structure, so that the problems of poor healing of a tendon-bone interface and high repeated fracture rate in a traditional repair method are solved, and the scaffold has good application prospects. The important application value is realized in the field of tendon-bone injury treatment in sports medicine.

The invention discloses a preparation method and application of exosome-loaded Cas13d-RNP, and belongs to the technical field of biliary atresia research. The method comprises the following steps: extracting exosomes: culturing human bile duct cells H69 in a DMEM / F12 culture medium until the cell density reaches 90%, collecting supernatant of the culture medium, and extracting the exosomes by an ultracentrifugation method; cas13d-RNP loading: the Cas13d protein and crRNA are mixed for 15 minutes at the room temperature, Cas13d-RNP is obtained, the exosome and the Cas13d-RNP are mixed, and the Cas13d-RNP is loaded into the exosome in a circulating freezing and thawing mode. According to the invention, Cas13d-RNP is accurately delivered through the exosome vector, specific targeting is carried out on bile duct cell CTGF mRNA, and expression of fibrosis-related factors in bile duct cells is significantly reduced. Compared with traditional medicine treatment, bile duct reaction and fibrosis processes can be more effectively inhibited. As a natural vector, the exosome has the advantages of low immunogenicity and high delivery efficiency, and immunoreactions possibly caused by a traditional virus vector are reduced.

The invention discloses application of neensinine in prevention and treatment of endometriosis, the neensinine is used as a traditional Chinese medicine monomer, the neensinine is used for preventing and treating the endometriosis, and the neensinine can resist fibrosis of the endometriosis so as to play a role in prevention and treatment. A mouse endometriosis model is constructed through an allotransplantation method, after four weeks of successful modeling, a modeling mouse model group, a dienogest control group and a neferine group are respectively taken for four weeks, and then laparotomy is performed to take out ectopic lesions. HE staining and masson staining methods are used for mutual evidence of fibrosis degree, immunohistochemical detection of fibrosis index a-SMA expression conditions is carried out, and Western-blot detection of fibrosis-related protein expression levels: FN, CTGF, alpha-SMA and COL-I. Therefore, the neferine can be potentially developed into drugs for treating or preventing fibrosis of endometriosis in the future.

The invention discloses an SS / CTGF composite biological material based on carboxyl chitosan as well as a preparation method and application of the SS / CTGF composite biological material. A substance containing active sericin (SS) and CTGF at the same time is extracted from genetic engineering silkworm cocoons over-expressing human connective tissue growth factors (CTGF) by utilizing an alkali extraction-acid protection method. Furthermore, the active substance, carboxyl chitosan and beta-glycerophosphate are used as raw materials, and the CH-SS / CTGF composite hydrogel biological material with the core-shell structure is prepared through thermal induction gelation. The material has the characteristics that the water content is adjustable, the mechanical property is controllable, and active protein can be quickly released. Compared with pure sericin, the active SS / CTGF substance and the composite hydrogel thereof can more remarkably promote cell proliferation and migration, and show good biocompatibility. The biological material prepared by the invention has wide application prospects in the fields of wound healing, drug delivery and regenerative medicine.

The invention discloses application of hyperoside to treatment of endometriosis, and aims to evaluate whether hyperoside has an anti-fibrosis effect when applied to a rat endometriosis model by taking fibrosis as a treatment target. The method comprises the following steps: constructing a rat endometriosis model through an autotransplantation method, dividing molded rats into a low-dose group, a medium-dose group, a high-dose group, a model group and a western medicine control group 4 weeks after successful modeling, and laparotomizing and taking out ectopic lesions after respectively taking medicines for 4 weeks. HE staining and masson staining methods are used for mutually proving the fibrosis degree, the expression conditions of fibrosis indexes a-SMA, Co-1, CTGF and Fn are detected in an immunohistochemical mode, and the anti-fibrosis effect of hyperoside is evaluated. As a result, hyperoside can prevent the development of fibrosis in endometriosis, and thus hyperoside can be used as a drug for treating or preventing fibrosis in endometriosis.

The present disclosure generally relates to anti-CTGF (connective tissue growth factor) antibodies or antigen-binding fragments thereof. Exemplary anti-CTGF antibodies disclosed herein address the need for treatments of conditions modulated by CTGF signaling. In some aspects, the anti-CTGF antibodies or antigen-binding fragments thereof are for diagnostic and / or therapeutic use, for example in a subject in need thereof, such as a human.

The invention provides an inhibitor of CTGF RNA, MITF RNA and SRD5A2RNA and application of the inhibitor. The inhibitor is a gene editing system. The gene editing system can knock down CTGF RNA, MITF RNA or SRD5A2RNA, and can be used for repairing scars, whitening skin, reducing or eliminating chloasma, preventing or treating melanoma, and / or preventing or treating androgenetic alopecia. The invention further provides a gene editing system, the gene editing system targets the AR RNA coded by the androgen receptor gene, and the gene editing system can be used for treating androgenetic alopecia and other diseases.

The present invention relates to a double-stranded oligonucleotide capable of inhibiting CTGF expression with extremely high specificity and high efficiency, a double-stranded oligonucleotide structure and a nanoparticle comprising the same, and use thereof in preventing or treating fibrosis or respiratory diseases.

This disclosure relates to treating a connective tissue injury. The treatment involves injecting, or otherwise administering, a therapeutic composition into an animal, such as a human being, in need thereof. The therapeutic composition may contain transforming growth factor beta 1 (TGF-β1) and / or transforming growth factor β 2 (TGF-β2), connective tissue growth factor (CTGF), and a pharmaceutically acceptable excipient or a secondary agent.

The preparation method of the HLC-CTGF fusion protein disclosed in the application comprises the following steps: designing a target gene and synthesizing and enzyme cutting sites, then reactivating a bacterial strain, and then sequentially performing expression vector construction of the fusion protein HLC-CTGF, expression of the fusion protein HLC-CTGF, purification of the fusion protein HLC-CTGF, and Western blotting of the fusion protein HLC-CTGF. The rhCol-CTGF fusion protein prepared in the application is non-toxic and has good cell compatibility. The fusion protein can be cross-linked to prepare a hydrogel scaffold, collagen provides necessary nutrition and structural support, and creates a good environment for the growth of osteoblasts; the degradation of the hydrogel is controlled to release CTGF, the time-dependent disadvantage of the expression of CTGF in the body is overcome, and the bone tissue repair is accelerated.

The invention discloses a meniscus organ construction method based on voxelization variable wire diameter printing and an obtained product. The method comprises the following steps: acquiring target meniscus medical image data and establishing a voxelization model; printing parameters are mapped according to mechanical distribution; the PCL stent is constructed by adopting a variable-wire-diameter melt extrusion technology, fibers are laid in a 0-degree / 60-degree / 120-degree three-axis staggered manner, the wire diameter is continuously adjustable in a range of 150-350 microns, and the porosity is from 80% to 10% in an inner region to 10% in an outer region; tGF-beta functional NorCol ink is injected into the inner area through a multi-nozzle system, and CTGF functional NorCol ink is injected into the outer area; packaging the hUC-MSCs at the density of 2 * 10 < 6 > cells / mL, performing cartilage differentiation culture for 28 days after 405nm light in-situ crosslinking, and applying periodic mechanical loading during the period. The invention also relates to the prepared meniscus organ and application of the meniscus organ in preparation of a graft. According to the scheme, continuous mechanical gradient reconstruction and regional biochemical microenvironment construction are realized, and the problems of insufficient mechanical bionics and limited regional induction in the prior art are solved.

This disclosure discloses inhibitors of CTGF RNA, MITF RNA, and SRD5A2 RNA and their applications, wherein the inhibitors are gene editing systems. The gene editing systems disclosed herein can knock down CTGF RNA, MITF RNA, or SRD5A2 RNA, and can be used to repair scars, whiten skin, reduce or eliminate melasma, prevent or treat melanoma, and / or prevent or treat androgenetic alopecia. This disclosure also discloses a gene editing system that targets AR RNA encoded by the androgen receptor gene, which can be used to treat androgenetic alopecia and other diseases.